FastQCFastQC Report
Mon 15 Aug 2016
SRR2920881_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920881_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4384034
Sequences flagged as poor quality0
Sequence length98
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA34600.042.4323651
GTATCAA57650.039.597251
TCAACGC75000.030.2713974
CAACGCA76250.029.654845
ATCAACG76050.029.5518973
AACGCAG80350.028.1371566
GTACATG130550.027.9210011
TATCAAC80800.027.8717122
TACATGG137250.025.8321552
ACATGGG143500.024.1314583
ACGCAGA96150.023.224397
CGCAGAG100800.021.9243348
ATACCGT3950.020.897516
CATGGGG103650.020.8862844
GAGTACA97900.018.5223541
AATACCG3651.9281288E-1017.5923445
GCAGAGT119000.017.3775799
AGTACAT97100.016.7215882
AGAGTAC98100.016.54603610-11
GTATTAG8850.016.1213381
Produced by FastQC (version 0.11.5)