##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920881_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4384034
Sequences flagged as poor quality	0
Sequence length	98
%GC	49
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.22857304482584	31.0	28.0	33.0	16.0	34.0
2	28.251072642228596	31.0	28.0	33.0	16.0	34.0
3	28.322945260004825	31.0	28.0	34.0	16.0	34.0
4	31.75471563404846	35.0	32.0	37.0	19.0	37.0
5	31.699383262082364	35.0	32.0	37.0	19.0	37.0
6	31.815154718234393	35.0	32.0	37.0	19.0	37.0
7	31.64124023673174	35.0	32.0	37.0	17.0	37.0
8	31.571677591916487	35.0	32.0	37.0	17.0	37.0
9	32.813549803673965	37.0	32.0	39.0	17.0	39.0
10-11	32.60181467570735	37.0	32.0	39.0	17.0	39.0
12-13	32.4178459838587	37.0	32.0	39.0	16.0	39.0
14-15	33.15349059792876	38.0	32.0	40.0	13.0	41.0
16-17	33.02820587157855	38.0	32.0	40.0	10.5	41.0
18-19	32.8891197011702	38.0	32.0	40.0	10.0	41.0
20-21	32.784046496902164	38.0	32.0	40.0	10.0	41.0
22-23	32.549494597897734	38.0	31.0	40.0	10.0	41.0
24-25	32.351065936076225	37.5	31.0	40.0	8.5	41.0
26-27	32.39389863308541	38.0	31.0	40.0	8.0	41.0
28-29	32.231315496184564	38.0	31.0	40.0	8.0	41.0
30-31	31.981002086206452	37.0	30.0	40.0	2.0	41.0
32-33	31.741515348649212	37.0	30.0	40.0	2.0	41.0
34-35	31.408704061145514	37.0	30.0	40.0	2.0	41.0
36-37	31.10208349205321	37.0	30.0	40.0	2.0	41.0
38-39	30.902863549872105	36.5	29.0	40.0	2.0	41.0
40-41	30.69955308284562	36.0	29.0	40.0	2.0	41.0
42-43	30.4640627787102	36.0	28.0	39.0	2.0	41.0
44-45	30.18738723285449	36.0	28.0	39.0	2.0	40.0
46-47	29.935220735058166	35.5	27.0	39.0	2.0	40.0
48-49	29.631364629015195	35.0	26.5	39.0	2.0	40.0
50-51	28.507748343192596	34.0	25.0	38.0	2.0	39.5
52-53	28.959650290120926	34.5	26.5	38.5	2.0	39.5
54-55	29.58806740549914	35.0	27.0	39.0	2.0	40.0
56-57	29.531023710126334	35.0	27.0	39.0	2.0	40.0
58-59	29.27945859908933	35.0	26.5	39.0	2.0	40.0
60-61	28.94293509128807	35.0	26.0	39.0	2.0	40.0
62-63	28.54201461485016	34.0	26.0	38.0	2.0	40.0
64-65	28.10850611560038	34.0	25.0	38.0	2.0	40.0
66-67	27.719661959738453	34.0	23.5	37.0	2.0	40.0
68-69	27.274463085824607	33.5	22.5	37.0	2.0	39.0
70-71	26.94492994351777	33.0	22.0	36.0	2.0	39.0
72-73	26.48381593299687	33.0	21.5	36.0	2.0	39.0
74-75	26.041840688279336	32.5	20.0	35.0	2.0	38.0
76-77	25.631388693609587	32.0	18.5	35.0	2.0	37.0
78-79	25.17424340230938	32.0	17.0	35.0	2.0	37.0
80-81	24.73984074941025	32.0	11.5	35.0	2.0	36.0
82-83	24.280935093112873	31.0	7.0	35.0	2.0	36.0
84-85	23.89587706208483	31.0	2.0	34.0	2.0	35.0
86-87	23.445730804095042	31.0	2.0	34.0	2.0	35.0
88-89	22.928459268335963	30.5	2.0	34.0	2.0	35.0
90-91	22.444672760293372	30.0	2.0	34.0	2.0	35.0
92-93	21.914782253057346	30.0	2.0	34.0	2.0	35.0
94-95	21.29258315514889	29.0	2.0	34.0	2.0	35.0
96-97	20.655884055643728	29.0	2.0	34.0	2.0	35.0
98	20.25991837654544	29.0	2.0	34.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	271019.0
3	43757.0
4	31628.0
5	25496.0
6	23720.0
7	23847.0
8	27525.0
9	31117.0
10	33644.0
11	35840.0
12	36256.0
13	35594.0
14	33748.0
15	34914.0
16	37429.0
17	39753.0
18	41098.0
19	41445.0
20	42439.0
21	45717.0
22	49046.0
23	54805.0
24	62171.0
25	71505.0
26	81908.0
27	94897.0
28	109393.0
29	126244.0
30	146859.0
31	171572.0
32	203167.0
33	246240.0
34	305009.0
35	385349.0
36	486931.0
37	525422.0
38	299351.0
39	28179.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.7565007922769	17.249287866714294	12.722043616446385	25.272167724562415
2	16.352129080685696	18.872643021495573	38.9369825883899	25.838245309428824
3	19.71007681549907	22.574083495504457	28.076874816060304	29.638964872936167
4	13.53108494336744	15.113757898079639	35.284134669786354	36.071022488766566
5	15.329043026799319	35.93889564343076	33.45782021186573	15.274241117904186
6	35.17426699173325	33.664126456559764	16.73762098167006	14.42398557003693
7	31.050177475537943	30.2842003996422	19.858051508171357	18.8075706166485
8	27.3946225116313	34.06329664012483	19.306399126754105	19.235681721489765
9	27.289044747371943	15.522507352817064	18.67754675260274	38.51090114720825
10-11	26.463590243773368	24.728634446243653	26.907428346195545	21.900346963787438
12-13	26.894086846452314	21.90659256495714	26.914137968830428	24.285182619760118
14-15	23.898706688846296	23.611588077061477	24.22655385241802	28.263151381674206
16-17	24.558733592727595	26.57140387011004	24.784165711826013	24.08569682533636
18-19	24.412214658406974	25.7891492470545	26.146651611293446	23.651984483245077
20-21	25.09364988707227	25.21317476427165	25.83634314140414	23.856832207251937
22-23	24.848934501771826	24.908115211965463	25.653696265736002	24.58925402052671
24-25	24.694689400987293	25.050236506442413	25.78139336576833	24.473680726801962
26-27	24.45515393996253	25.36195336632923	25.695051014672387	24.48784167903585
28-29	24.467646860130948	25.417645764636713	25.82526091020964	24.289446465022696
30-31	24.55102167266742	25.23533941856669	25.640970927649864	24.57266798111602
32-33	24.55553317197382	25.239706486602287	25.5456948653407	24.659065476083196
34-35	24.85180658131095	25.2694912152793	25.37666172946088	24.50204047394887
36-37	24.946880625340057	24.973099252694603	25.461107354674635	24.61891276729071
38-39	24.63897105030479	25.121901414551438	25.553701480647263	24.68542605449651
40-41	24.76125612438163	24.999131902961537	25.67468902930478	24.564922943352055
42-43	24.81294727198879	24.893000639373152	25.47422084558073	24.819831243057326
44-45	24.542285951885244	25.067273925085836	25.587612642814307	24.802827480214614
46-47	24.629971959253144	25.065590063221364	25.445451889798846	24.858986087726645
48-49	24.677961312076217	25.01279544305896	25.48193878843242	24.827304456432405
50-51	24.49778215000866	25.12529040547594	25.585095974780593	24.791831469734802
52-53	24.596885894414612	25.046289984613924	25.53919440189628	24.817629719075185
54-55	24.28806862999503	24.855723376576506	25.777205708396988	25.07900228503147
56-57	24.225393828622842	25.01179120755022	25.924495955942227	24.838319007884703
58-59	24.32910431045184	24.76700446019379	25.88086193462566	25.023029294728712
60-61	24.33159719736688	24.805112772482197	26.033770182938525	24.8295198472124
62-63	24.197499196992485	24.86355170366418	25.891813560263355	25.047135539079985
64-65	24.101921896249888	24.693451722548648	25.90815403352816	25.29647234767331
66-67	24.072676520501577	24.8274127438297	25.8408894806741	25.259021254994618
68-69	24.000937733918114	24.596046033710778	26.074614667862615	25.32840156450849
70-71	23.940615925579188	24.513022062168606	26.099141814358713	25.447220197893493
72-73	24.037857532681116	24.534861840105197	26.00899165619723	25.418288971016455
74-75	23.96452375560031	24.64315873569731	25.97615576515293	25.41616174354945
76-77	24.075781970339925	24.48212056401495	26.0304918165236	25.411605649121526
78-79	24.01034894502146	24.458434735798182	26.098757169887627	25.432459149292736
80-81	23.88197118045187	24.618558239708584	26.168338328039514	25.331132251800035
82-83	23.969625898674558	24.512126841756366	26.081396658377315	25.436850601191765
84-85	23.93553250928805	24.357859627850836	26.169906205610587	25.536701657250525
86-87	23.97791423427349	24.213973621725245	26.321285431938442	25.486826712062822
88-89	23.968502455014303	24.16464944264169	26.32353708898774	25.543311013356266
90-91	23.846609307842538	24.237317604124307	26.448565172856515	25.46750791517664
92-93	23.988345928210745	23.98709843849767	26.424521664947843	25.600033968343748
94-95	23.857323159342556	23.96521016161418	26.54399107969297	25.633475599350298
96-97	24.00959651794872	23.86852848240896	26.48438196577077	25.63749303387155
98	24.02710308484032	23.81882382994854	26.36759514486613	25.786477940345016
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	56.0
1	48.5
2	80.5
3	137.5
4	144.5
5	163.5
6	263.5
7	520.5
8	823.5
9	958.0
10	994.0
11	1113.5
12	1299.0
13	1543.0
14	1796.0
15	1999.5
16	2172.0
17	2222.5
18	2206.0
19	2204.5
20	2174.0
21	2188.5
22	2256.5
23	2451.0
24	2793.0
25	3401.5
26	4429.5
27	5715.5
28	7558.5
29	10480.5
30	14919.0
31	21084.0
32	28274.0
33	36408.5
34	45426.5
35	55688.0
36	66971.5
37	78305.5
38	88863.5
39	99957.0
40	113396.5
41	126670.5
42	139675.5
43	150189.0
44	159388.5
45	169270.5
46	178205.5
47	188614.5
48	197455.5
49	201331.0
50	202144.0
51	203512.5
52	206013.5
53	205352.0
54	200433.5
55	193050.0
56	180082.0
57	165273.5
58	150586.0
59	131478.5
60	110657.0
61	91690.5
62	75973.5
63	60659.5
64	45781.0
65	34175.0
66	26368.0
67	20622.0
68	14992.0
69	10982.5
70	8388.5
71	6736.5
72	5399.5
73	3853.5
74	2923.5
75	2018.5
76	1146.5
77	637.0
78	350.5
79	207.5
80	131.5
81	83.0
82	48.0
83	28.0
84	26.5
85	19.5
86	14.0
87	15.0
88	13.0
89	9.0
90	6.5
91	6.5
92	6.5
93	8.0
94	6.5
95	3.5
96	2.5
97	0.5
98	0.5
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.38489664998036055
2	0.023699633716344353
3	0.018339273828624506
4	0.015738929032028493
5	0.022239791023518522
6	0.021258959214276167
7	0.013982555792222415
8	0.008873106367332005
9	0.0
10-11	6.843012622621083E-5
12-13	0.005463005077059165
14-15	0.002999520532915575
16-17	0.005269119719418234
18-19	0.035252920027536286
20-21	0.07655050120505452
22-23	0.09645226291584418
24-25	0.06291009604396317
26-27	0.07268419907327361
28-29	0.05588460308473885
30-31	0.050740938596735334
32-33	0.01963944622692251
34-35	0.3382387089151225
36-37	0.5162140622084591
38-39	0.4715291897827435
40-41	0.47998031037168054
42-43	0.42930780190117135
44-45	0.404729981564924
46-47	0.4039544401343602
48-49	0.3933591755903353
50-51	0.389584113626856
52-53	0.44933501884337573
54-55	0.4507606464730885
56-57	0.42182610810043897
58-59	0.4072961112984069
60-61	0.42190594324770286
62-63	0.4022893070628558
64-65	0.44873055272837753
66-67	0.469635956290485
68-69	0.4149146653515917
70-71	0.3725107971334164
72-73	0.3595318831925117
74-75	0.3414549248477544
76-77	0.3397441716920991
78-79	0.339709956628986
80-81	0.355277810345449
82-83	0.3507728270355568
84-85	0.35087547222489607
86-87	0.33746316748455873
88-89	0.3796959603871685
90-91	0.3577869149737433
92-93	0.34809264709169685
94-95	0.35525500030337354
96-97	0.35525500030337354
98	0.35081844711970755
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
98	4384034.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.5014219029761
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.77480158493537	37.762338025521466
2	11.52091550983164	11.63645229741097
3	4.972362489363087	7.533341277895731
4	2.579245917805962	5.210223451465907
5	1.586467949296647	4.005944362148963
6	1.027568502676409	3.1136202292722444
7	0.6736558135835479	2.3814403521422753
8	0.5067097585541249	2.047165063927761
9	0.3577255043957182	1.6259081960648785
>10	1.8556691705189317	16.36920864604674
>50	0.100092897701741	3.434932756116402
>100	0.0424161444446392	3.7999885204794754
>500	0.001776567806070302	0.5899612224531352
>1k	5.921890860467913E-4	0.4894755990541415
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	1.1405021037701807E-5
16-17	0.0	0.0	0.0	0.0	3.421506311310542E-5
18-19	0.0	0.0	0.0	2.2810042075403613E-5	4.5620084150807227E-5
20-21	0.0	0.0	0.0	5.702510518850903E-5	4.5620084150807227E-5
22-23	0.0	0.0	0.0	1.3686025245242167E-4	4.5620084150807227E-5
24-25	2.2810042075403613E-5	0.0	0.0	4.219857783949668E-4	4.5620084150807227E-5
26-27	2.2810042075403613E-5	0.0	0.0	9.694267882046536E-4	4.5620084150807227E-5
28-29	2.2810042075403613E-5	0.0	0.0	0.004983994193475689	4.5620084150807227E-5
30-31	2.2810042075403613E-5	0.0	0.0	0.021452844571917096	4.5620084150807227E-5
32-33	2.2810042075403613E-5	0.0	0.0	0.055359972117004566	6.843012622621083E-5
34-35	2.2810042075403613E-5	0.0	0.0	0.10279345461280638	6.843012622621083E-5
36-37	2.2810042075403613E-5	0.0	0.0	0.18025635750087704	6.843012622621083E-5
38-39	2.2810042075403613E-5	0.0	0.0	0.3139117990417045	6.843012622621083E-5
40-41	2.2810042075403613E-5	1.1405021037701807E-5	0.0	0.4677199127561511	6.843012622621083E-5
42-43	3.421506311310542E-5	2.2810042075403613E-5	0.0	0.6285421144087842	6.843012622621083E-5
44-45	4.5620084150807227E-5	2.2810042075403613E-5	0.0	0.8205912636626449	6.843012622621083E-5
46-47	4.5620084150807227E-5	2.2810042075403613E-5	0.0	1.0511095488766737	6.843012622621083E-5
48-49	4.5620084150807227E-5	2.2810042075403613E-5	0.0	1.3007996744550794	6.843012622621083E-5
50-51	4.5620084150807227E-5	2.2810042075403613E-5	0.0	1.5786145819124577	6.843012622621083E-5
52-53	4.5620084150807227E-5	2.2810042075403613E-5	0.0	1.8646753195800945	6.843012622621083E-5
54-55	4.5620084150807227E-5	2.2810042075403613E-5	0.0	2.1787239788742516	7.983514726391265E-5
56-57	4.5620084150807227E-5	2.2810042075403613E-5	0.0	2.5419282788409028	9.124016830161445E-5
58-59	4.5620084150807227E-5	2.2810042075403613E-5	0.0	2.935412909662653	9.124016830161445E-5
60-61	4.5620084150807227E-5	2.2810042075403613E-5	0.0	3.3618922663464748	9.124016830161445E-5
62-63	4.5620084150807227E-5	2.2810042075403613E-5	0.0	3.7892954297343495	9.124016830161445E-5
64-65	4.5620084150807227E-5	2.2810042075403613E-5	0.0	4.245530486305535	9.124016830161445E-5
66-67	6.843012622621083E-5	2.2810042075403613E-5	0.0	4.767960284979542	9.124016830161445E-5
68-69	6.843012622621083E-5	4.5620084150807227E-5	0.0	5.339956305083401	9.124016830161445E-5
70-71	6.843012622621083E-5	4.5620084150807227E-5	0.0	5.953865777500813	9.124016830161445E-5
72-73	6.843012622621083E-5	4.5620084150807227E-5	0.0	6.570455429862086	9.124016830161445E-5
74-75	6.843012622621083E-5	4.5620084150807227E-5	0.0	7.2123186088429065	9.124016830161445E-5
76-77	6.843012622621083E-5	4.5620084150807227E-5	0.0	7.907066870375549	9.124016830161445E-5
78-79	6.843012622621083E-5	4.5620084150807227E-5	0.0	8.662375793618388	1.0264518933931626E-4
80-81	6.843012622621083E-5	4.5620084150807227E-5	0.0	9.45559500679055	1.1405021037701806E-4
82-83	6.843012622621083E-5	4.5620084150807227E-5	0.0	10.25100398400195	1.1405021037701806E-4
84-85	6.843012622621083E-5	4.5620084150807227E-5	0.0	11.05861633372369	1.1405021037701806E-4
86	6.843012622621083E-5	4.5620084150807227E-5	0.0	11.679174933406083	1.1405021037701806E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	3460	0.0	42.432365	1
GTATCAA	5765	0.0	39.59725	1
TCAACGC	7500	0.0	30.271397	4
CAACGCA	7625	0.0	29.65484	5
ATCAACG	7605	0.0	29.551897	3
AACGCAG	8035	0.0	28.137156	6
GTACATG	13055	0.0	27.921001	1
TATCAAC	8080	0.0	27.871712	2
TACATGG	13725	0.0	25.832155	2
ACATGGG	14350	0.0	24.131458	3
ACGCAGA	9615	0.0	23.22439	7
CGCAGAG	10080	0.0	21.924334	8
ATACCGT	395	0.0	20.89751	6
CATGGGG	10365	0.0	20.886284	4
GAGTACA	9790	0.0	18.522354	1
AATACCG	365	1.9281288E-10	17.592344	5
GCAGAGT	11900	0.0	17.377579	9
AGTACAT	9710	0.0	16.721588	2
AGAGTAC	9810	0.0	16.546036	10-11
GTATTAG	885	0.0	16.121338	1
>>END_MODULE