FastQCFastQC Report
Mon 15 Aug 2016
SRR2920881_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920881_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4384034
Sequences flagged as poor quality0
Sequence length101
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA57400.045.816361
GGTATCA30200.045.1941571
TCAACGC74300.033.991114
ATCAACG75200.033.58433
CAACGCA76700.032.800345
TATCAAC80500.031.9603232
AACGCAG80450.031.567526
GTACATG133500.026.8238451
TACATGG136800.025.1223492
ACGCAGA100500.024.7017977
CGCAGAG103400.023.7305778
ACATGGG143450.023.0330373
GCAGAGT122650.019.1547269
GTGTACG4350.018.585191
CATGGGG102300.018.5156844
GAGTACA102800.018.0417561
AGAGTAC101300.017.91948710-11
AGTACAT101000.017.5305392
GTATAGA10950.016.9378321
GCACCGT10450.016.807316