##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920881_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4384034
Sequences flagged as poor quality	0
Sequence length	101
%GC	49
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.157932853622942	31.0	30.0	34.0	26.0	34.0
2	30.167296832095737	31.0	30.0	34.0	26.0	34.0
3	30.30699214467771	31.0	30.0	34.0	26.0	34.0
4	33.9364861221423	35.0	35.0	37.0	30.0	37.0
5	33.44253876680701	35.0	35.0	37.0	28.0	37.0
6	33.37943592590751	35.0	35.0	37.0	28.0	37.0
7	33.184655274115116	35.0	33.0	37.0	27.0	37.0
8	33.11521032911698	35.0	33.0	37.0	26.0	37.0
9	34.44111199867519	38.0	34.0	39.0	27.0	39.0
10-11	34.24016887186551	37.5	34.0	39.0	26.5	39.0
12-13	34.05291416535547	37.0	34.0	39.0	25.5	39.0
14-15	34.94917979650705	38.0	34.0	40.0	25.0	41.0
16-17	34.818889520473604	38.0	33.5	40.0	25.0	41.0
18-19	34.71127653207069	38.0	33.5	40.0	25.0	41.0
20-21	34.58529199362961	38.0	34.0	40.0	24.0	41.0
22-23	34.430937693457665	38.0	33.0	40.0	23.5	41.0
24-25	34.25004333907994	38.0	33.0	40.0	22.5	41.0
26-27	34.06510955435108	38.0	33.0	40.0	21.5	41.0
28-29	34.006183004055174	38.0	33.0	40.0	21.0	41.0
30-31	34.00637517865965	38.0	33.0	40.0	21.0	41.0
32-33	33.86731067779128	38.0	33.0	40.0	19.0	41.0
34-35	33.69001198439611	38.0	33.0	40.0	18.5	41.0
36-37	33.5055942768692	38.0	32.0	40.0	18.0	41.0
38-39	33.32320997054311	38.0	32.0	40.0	16.0	41.0
40-41	33.09749069008133	38.0	32.0	40.0	15.5	41.0
42-43	33.132294252279976	38.0	32.0	40.0	15.0	41.0
44-45	32.98931144238389	38.0	32.0	40.0	14.5	41.0
46-47	32.75122090750209	37.5	31.0	40.0	10.5	41.0
48-49	32.486873048886025	37.0	31.0	40.0	9.0	41.0
50-51	32.22079071467055	37.0	31.0	40.0	8.0	41.0
52-53	31.94751831304228	37.0	30.5	40.0	7.0	41.0
54-55	31.652951254483884	36.0	30.0	39.5	4.0	41.0
56-57	31.31316956027257	36.0	30.0	39.0	2.0	41.0
58-59	30.967488162728664	35.0	29.0	39.0	2.0	40.0
60-61	30.618375336505146	35.0	29.0	39.0	2.0	40.0
62-63	30.277254008522746	35.0	28.0	38.5	2.0	40.0
64-65	29.851739744719133	34.5	28.0	38.0	2.0	40.0
66-67	29.40742715499013	34.0	28.0	37.5	2.0	40.0
68-69	28.95427031359702	34.0	27.0	37.0	2.0	39.0
70-71	28.518862079992992	34.0	26.0	36.0	2.0	39.0
72-73	28.06799422632215	33.5	26.0	36.0	2.0	39.0
74-75	27.620568065849852	33.0	26.0	35.0	2.0	38.0
76-77	26.20097323606523	31.5	24.0	34.0	2.0	36.0
78-79	27.02631834059681	32.5	25.0	35.0	2.0	37.0
80-81	27.04795035804923	33.0	25.5	35.0	2.0	36.0
82-83	26.717990097704533	33.0	25.0	35.0	2.0	36.0
84-85	26.40329158031165	33.0	24.5	35.0	2.0	35.5
86-87	25.864962383959615	32.0	23.0	35.0	2.0	35.0
88-89	25.496781959264002	32.0	20.5	35.0	2.0	35.0
90-91	25.1286016486186	32.0	19.5	34.0	2.0	35.0
92-93	24.80871293425188	32.0	18.0	34.0	2.0	35.0
94-95	24.41990002814759	31.5	13.5	34.0	2.0	35.0
96-97	23.871932676617014	31.0	2.0	34.0	2.0	35.0
98-99	23.247133576062595	31.0	2.0	34.0	2.0	35.0
100-101	21.046618023491604	28.0	2.0	33.0	2.0	34.5
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	116450.0
3	51480.0
4	26562.0
5	18919.0
6	16301.0
7	16113.0
8	17968.0
9	20707.0
10	23184.0
11	25565.0
12	28216.0
13	30787.0
14	33109.0
15	35452.0
16	36942.0
17	38161.0
18	37901.0
19	36825.0
20	37409.0
21	38633.0
22	42056.0
23	47335.0
24	54583.0
25	64553.0
26	76773.0
27	90500.0
28	107323.0
29	126148.0
30	147457.0
31	175397.0
32	209686.0
33	256881.0
34	322159.0
35	418259.0
36	557157.0
37	639544.0
38	340403.0
39	21136.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.84547765681549	18.56706154877033	13.367813528132494	25.219647266281687
2	16.46784673659009	19.934653791462384	37.68659184668732	25.910907625260204
3	19.436897717792295	23.424322180501985	28.49813308342255	28.640647018283165
4	13.992546038603008	16.105497941001147	34.77001287344805	35.1319431469478
5	15.269907069243427	36.23259287714882	33.51547804203487	14.982022011572887
6	35.0606539723077	34.259972214534486	16.45596935378241	14.223404459375402
7	30.849827878045826	30.444912979617193	19.8899881444865	18.815270997850476
8	27.364607697081	34.41391229039421	19.16569011836656	19.055789894158227
9	27.102096031205413	15.436317958600196	18.586418170994737	38.875167839199655
10-11	26.414454693603133	24.911758580244882	26.92547390940548	21.748312816746505
12-13	26.82541058171733	21.964998602972248	26.991455351232872	24.218135464077548
14-15	23.85543164698725	23.708669570316996	24.183747992626383	28.25215079006937
16-17	24.539060611518057	26.800143072390565	24.71702230473004	23.943774011361334
18-19	24.435531396805754	25.898456775010075	26.070573028758677	23.595438799425494
20-21	25.105685788153792	25.295573359377315	25.722515191690263	23.876225660778626
22-23	24.826733713037516	25.036185132727525	25.674847017934127	24.462234136300832
24-25	24.6723667921662	25.206405497390804	25.753271082643455	24.367956627799547
26-27	24.46831360447015	25.54416054562138	25.60889373804513	24.37863211186334
28-29	24.518126547304004	25.52253778279908	25.722972767905937	24.236362901990976
30-31	24.62693028803237	25.3253105373064	25.552316345638847	24.49544282902238
32-33	24.61295576526489	25.33332009910158	25.501155257161656	24.552568878471874
34-35	24.801672041803783	25.376921223669584	25.411097604824302	24.41030912970233
36-37	24.918069441338996	25.092094876088872	25.4676498637913	24.522185818780827
38-39	24.64771924780755	25.19169316316251	25.59474793247472	24.565839656555216
40-41	24.734574230462623	25.02542803284905	25.74887781265529	24.49111992403304
42-43	24.747273750336067	24.967328805163334	25.534986894450796	24.750410550049807
44-45	24.454324726171272	25.13936570507895	25.601284657183466	24.805024911566306
46-47	24.55658489162726	25.122689940799642	25.471509864082993	24.849215303490105
48-49	24.566344532979535	25.033856031141276	25.57584908690515	24.82395034897404
50-51	24.42523969686192	25.155446212473613	25.63468024106682	24.784633849597647
52-53	24.59639375435702	25.05804420347248	25.51122013044605	24.83434191172445
54-55	24.294190467597566	24.921651494669906	25.668470302965048	25.115687734767484
56-57	24.219122553992793	25.103263662083318	25.738317362609287	24.939296421314598
58-59	24.282371018166966	24.934501268116584	25.731241403843892	25.051886309872557
60-61	24.32221358020945	25.02788627192315	25.7677023925041	24.882197755363304
62-63	24.12340744225114	25.13116523363369	25.578982811588574	25.1664445125266
64-65	23.959193524398025	24.980564434374855	25.615448172281237	25.444793868945894
66-67	23.976892030073405	25.03594646629293	25.54679589823493	25.440365605398735
68-69	23.911267611100417	24.874103277664116	25.719221484851868	25.4954076263836
70-71	23.8289476836309	24.82108899178539	25.635317456243317	25.71464586834039
72-73	23.962963170038268	24.81567561418356	25.47334086943762	25.74802034634055
74-75	23.847013318734124	24.935687075271005	25.351803341715097	25.865496264279773
76-77	23.89883844424497	24.806675106819615	25.545925945489785	25.748560503445635
78-79	23.844329839718004	24.81566463119115	25.52646784007276	25.81353768901809
80-81	23.699235430156946	24.96446129343781	25.536171912661132	25.80013136374411
82-83	23.734483528752335	24.886910808678113	25.431545707789255	25.947059954780293
84-85	23.64795623891566	24.715976363758312	25.43364363771086	26.20242375961517
86-87	23.680011709621958	24.66399731502028	25.55222474240833	26.10376623294944
88-89	23.655704311242097	24.64837718860819	25.442582055843044	26.253336444306676
90-91	23.444757322677177	24.779452865626116	25.494372766053992	26.281417045642712
92-93	23.519196185760148	24.54591674159752	25.46187774581135	26.473009326830983
94-95	23.291304947340677	24.54563277696313	25.526065746399706	26.636996529296486
96-97	23.41651448932455	24.532207933842862	25.457475828124743	26.593801748707847
98-99	23.335859243107564	24.51792342843665	25.381287296992927	26.764930031462857
100-101	23.36671773688485	24.270344665234205	25.502964471620558	26.859973126260385
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	42.0
1	48.0
2	64.5
3	86.0
4	87.5
5	98.5
6	167.5
7	337.5
8	492.5
9	522.5
10	478.0
11	421.5
12	426.0
13	458.0
14	504.0
15	575.0
16	655.5
17	729.5
18	794.0
19	890.5
20	1001.0
21	1184.0
22	1441.5
23	1792.5
24	2203.0
25	2669.5
26	3404.0
27	4510.0
28	6041.5
29	8301.0
30	11694.0
31	16579.5
32	23032.0
33	30285.5
34	38222.5
35	46478.5
36	56555.5
37	68131.5
38	78810.5
39	88626.0
40	99230.5
41	112450.0
42	126343.0
43	139104.0
44	148623.5
45	157596.5
46	166934.5
47	176091.0
48	186860.5
49	195360.0
50	199306.5
51	201640.0
52	203603.5
53	204551.0
54	202777.0
55	195067.5
56	182176.5
57	167524.5
58	153531.5
59	134824.5
60	113354.5
61	93759.0
62	76310.0
63	61096.5
64	46857.5
65	34896.5
66	26065.5
67	20465.0
68	15482.5
69	11039.5
70	8261.5
71	6592.5
72	5328.5
73	4095.5
74	3047.5
75	2214.0
76	1346.5
77	683.0
78	357.5
79	174.5
80	88.5
81	50.5
82	21.5
83	7.5
84	5.5
85	4.5
86	4.0
87	3.0
88	1.5
89	0.5
90	0.5
91	0.0
92	0.0
93	0.5
94	0.5
95	0.0
96	0.0
97	0.5
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.19119377267603307
2	0.0
3	0.013754455371468377
4	0.013526354950714343
5	0.007755414305637229
6	0.10871266053137361
7	0.14425070608485244
8	0.12584300213000174
9	0.14443318642145567
10-11	0.11719799618342376
12-13	0.11620575935314371
14-15	0.11811039786643991
16-17	0.06922847769884996
18-19	0.08130639497777618
20-21	0.03886831169648775
22-23	0.08626757912917646
24-25	0.05953420981680343
26-27	0.06783706513225034
28-29	0.041400226366857557
30-31	0.025239311556434097
32-33	0.033302661430089274
34-35	0.020300937447109212
36-37	0.02404178434747541
38-39	0.03145504802198158
40-41	0.024543605273134286
42-43	0.013355279635148815
44-45	0.028067756773784144
46-47	0.03520729994338548
48-49	0.00908980176704834
50-51	0.015624878821651474
52-53	0.006979872875073505
54-55	0.013104369172319377
56-57	0.005280524740455936
58-59	0.0038891121738563158
60-61	0.024018974305400002
62-63	0.010059228555252993
64-65	0.007253593379978349
66-67	0.04158270670346079
68-69	0.09249472061576164
70-71	0.02255913161257417
72-73	0.04601925988712679
74-75	0.08868544358916924
76-77	0.24841276322218306
78-79	0.08136342008296468
80-81	0.017905883029191837
82-83	0.012477093015245775
84-85	0.013594785076940553
86-87	0.26371830145477887
88-89	0.6252802783920015
90-91	0.26747055337618275
92-93	0.354969874777431
94-95	0.0011975272089586896
96-97	0.003843492089705509
98-99	0.003079355680179488
100-101	0.004573413436118424
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4384034.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.50150507779205
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.28766940021116	33.14983920597881
2	12.975909947194667	12.067986845968903
3	5.577542286759568	7.780923329080498
4	3.0019443972641895	5.583797305305203
5	1.7878708328079636	4.156934230512792
6	1.1805988842211206	3.2939775005666436
7	0.8108051263880228	2.6392561091282722
8	0.5858161834372645	2.1793067383008573
9	0.43321582633940703	1.8130669153471637
>10	2.1797295099765943	17.835300391054936
>50	0.1227527286126635	3.8774623191884574
>100	0.053141429873365405	4.3462769233198655
>500	0.0021664209173734954	0.6656287257252141
>1k	8.370259965696873E-4	0.6102434605223959
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	1.1405021037701807E-5	0.0	0.0	2.2810042075403613E-5	0.0
20-21	2.2810042075403613E-5	0.0	0.0	5.702510518850903E-5	0.0
22-23	2.2810042075403613E-5	0.0	0.0	1.3686025245242167E-4	0.0
24-25	2.2810042075403613E-5	0.0	0.0	4.219857783949668E-4	0.0
26-27	2.2810042075403613E-5	0.0	0.0	9.580217671669517E-4	0.0
28-29	2.2810042075403613E-5	0.0	0.0	0.004710273688570846	0.0
30-31	2.2810042075403613E-5	0.0	0.0	0.02077994833069269	0.0
32-33	3.421506311310542E-5	0.0	0.0	0.054550215623327736	1.1405021037701807E-5
34-35	4.5620084150807227E-5	0.0	0.0	0.10183543284563942	2.2810042075403613E-5
36-37	4.5620084150807227E-5	0.0	0.0	0.18024495247983935	2.2810042075403613E-5
38-39	4.5620084150807227E-5	0.0	0.0	0.3144136199673634	2.2810042075403613E-5
40-41	4.5620084150807227E-5	0.0	0.0	0.4699667019005783	2.2810042075403613E-5
42-43	4.5620084150807227E-5	0.0	0.0	0.6329672625714124	2.2810042075403613E-5
44-45	4.5620084150807227E-5	2.2810042075403613E-5	0.0	0.8267271649809285	3.421506311310542E-5
46-47	6.843012622621083E-5	2.2810042075403613E-5	0.0	1.0585798376563686	4.5620084150807227E-5
48-49	6.843012622621083E-5	2.2810042075403613E-5	0.0	1.3079506226457185	4.5620084150807227E-5
50-51	6.843012622621083E-5	2.2810042075403613E-5	0.0	1.583005515011973	4.5620084150807227E-5
52-53	6.843012622621083E-5	2.2810042075403613E-5	0.0	1.8661237572518825	4.5620084150807227E-5
54-55	6.843012622621083E-5	2.2810042075403613E-5	0.0	2.176454379687749	4.5620084150807227E-5
56-57	6.843012622621083E-5	2.2810042075403613E-5	0.0	2.5360204779433735	4.5620084150807227E-5
58-59	6.843012622621083E-5	2.2810042075403613E-5	0.0	2.926323107895605	4.5620084150807227E-5
60-61	6.843012622621083E-5	2.2810042075403613E-5	0.0	3.349791539025473	4.5620084150807227E-5
62-63	6.843012622621083E-5	2.2810042075403613E-5	0.0	3.7761796555409926	4.5620084150807227E-5
64-65	6.843012622621083E-5	2.2810042075403613E-5	0.0	4.229335356431998	4.5620084150807227E-5
66-67	6.843012622621083E-5	2.2810042075403613E-5	0.0	4.7473057918802635	6.843012622621083E-5
68-69	9.124016830161445E-5	2.2810042075403613E-5	0.0	5.312755329908482	6.843012622621083E-5
70-71	9.124016830161445E-5	2.2810042075403613E-5	0.0	5.920825431554591	6.843012622621083E-5
72-73	9.124016830161445E-5	2.2810042075403613E-5	0.0	6.533788287225875	6.843012622621083E-5
74-75	9.124016830161445E-5	2.2810042075403613E-5	0.0	7.175537415996317	6.843012622621083E-5
76-77	9.124016830161445E-5	2.2810042075403613E-5	0.0	7.8709927888332984	6.843012622621083E-5
78-79	9.124016830161445E-5	3.421506311310542E-5	0.0	8.624659389046709	6.843012622621083E-5
80-81	9.124016830161445E-5	4.5620084150807227E-5	0.0	9.41762769175604	6.843012622621083E-5
82-83	9.124016830161445E-5	4.5620084150807227E-5	0.0	10.21388064052423	7.983514726391265E-5
84-85	9.124016830161445E-5	4.5620084150807227E-5	0.0	11.021869355940215	9.124016830161445E-5
86-87	9.124016830161445E-5	4.5620084150807227E-5	0.0	11.863183542828363	9.124016830161445E-5
88-89	9.124016830161445E-5	4.5620084150807227E-5	0.0	12.76436268514341	9.124016830161445E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	5740	0.0	45.81636	1
GGTATCA	3020	0.0	45.194157	1
TCAACGC	7430	0.0	33.99111	4
ATCAACG	7520	0.0	33.5843	3
CAACGCA	7670	0.0	32.80034	5
TATCAAC	8050	0.0	31.960323	2
AACGCAG	8045	0.0	31.56752	6
GTACATG	13350	0.0	26.823845	1
TACATGG	13680	0.0	25.122349	2
ACGCAGA	10050	0.0	24.701797	7
CGCAGAG	10340	0.0	23.730577	8
ACATGGG	14345	0.0	23.033037	3
GCAGAGT	12265	0.0	19.154726	9
GTGTACG	435	0.0	18.58519	1
CATGGGG	10230	0.0	18.515684	4
GAGTACA	10280	0.0	18.041756	1
AGAGTAC	10130	0.0	17.919487	10-11
AGTACAT	10100	0.0	17.530539	2
GTATAGA	1095	0.0	16.937832	1
GCACCGT	1045	0.0	16.80731	6
>>END_MODULE