FastQCFastQC Report
Mon 15 Aug 2016
SRR2920880_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920880_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3525421
Sequences flagged as poor quality0
Sequence length98
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA58250.042.3504071
TCAACGC67650.035.929694
ATCAACG69300.035.0742233
GGTATCA32300.034.7677081
CAACGCA70200.034.4938965
TATCAAC72050.033.7996442
AACGCAG73900.032.637196
ACGCAGA88400.026.7624477
GTACATG105600.026.3681831
CGCAGAG88900.025.732628
TACATGG108350.025.2271942
ACATGGG115950.022.8613833
GCAGAGT105450.020.5201559
CATGGGG76150.019.994644
GAGTACA80600.019.75741
CGCCGTA3851.8189894E-1219.1235192
GTGTAGC20750.018.631591
AGTACAT78850.018.6122912
AGAGTAC85950.017.62906610-11
GAGTACT40650.016.35815812-13
Produced by FastQC (version 0.11.5)