##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920880_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3525421
Sequences flagged as poor quality	0
Sequence length	98
%GC	47
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.60526671849972	31.0	30.0	33.0	22.0	34.0
2	28.632842999460205	31.0	28.0	34.0	16.0	34.0
3	28.709443212597872	31.0	28.0	34.0	20.0	34.0
4	32.185654706203884	35.0	32.0	37.0	22.0	37.0
5	32.15596208225911	35.0	32.0	37.0	22.0	37.0
6	32.28190079993283	35.0	32.0	37.0	23.0	37.0
7	32.118905231460296	35.0	32.0	37.0	22.0	37.0
8	32.06247395701109	35.0	32.0	37.0	22.0	37.0
9	33.33021531329166	37.0	33.0	39.0	22.0	39.0
10-11	33.14197694403023	37.0	32.5	39.0	19.0	39.0
12-13	32.97427059066137	37.0	32.0	39.0	18.0	39.0
14-15	33.74297325057064	38.0	32.5	40.0	17.0	41.0
16-17	33.64563310311024	38.0	32.0	40.0	17.0	41.0
18-19	33.54011662720566	38.0	32.0	40.0	17.5	41.0
20-21	33.4524902132256	38.0	32.0	40.0	17.5	41.0
22-23	33.23651274556997	38.0	32.0	40.0	16.0	41.0
24-25	33.05418246501624	38.0	32.0	40.0	15.0	41.0
26-27	33.09557610282573	38.0	32.0	40.0	12.0	41.0
28-29	32.94803840449127	38.0	32.0	40.0	10.0	41.0
30-31	32.713597042736176	38.0	31.0	40.0	9.5	41.0
32-33	32.478227281224	37.5	31.0	40.0	9.0	41.0
34-35	32.16072704508199	37.0	30.5	40.0	8.0	41.0
36-37	31.87399320535051	37.0	30.0	40.0	6.5	41.0
38-39	31.691690864722254	37.0	30.0	40.0	2.0	41.0
40-41	31.512196557517527	37.0	30.0	40.0	2.0	41.0
42-43	31.29313534468649	37.0	30.0	40.0	2.0	41.0
44-45	31.02988607601759	36.0	29.5	40.0	2.0	41.0
46-47	30.799832275350944	36.0	29.0	39.0	2.0	41.0
48-49	30.510331957516563	36.0	28.0	39.0	2.0	40.0
50-51	29.386217844620543	34.5	26.5	38.0	2.0	39.5
52-53	29.839140630296352	35.0	28.0	38.5	2.0	39.5
54-55	30.502293484948325	36.0	28.0	39.0	2.0	40.0
56-57	30.46954051728857	36.0	28.0	39.0	2.0	41.0
58-59	30.241525763873305	35.5	28.0	39.0	2.0	41.0
60-61	29.930425898070048	35.0	28.0	39.0	2.0	40.5
62-63	29.552579535890892	35.0	27.5	39.0	2.0	40.0
64-65	29.15020957780645	34.5	26.5	38.5	2.0	40.0
66-67	28.776272535960956	34.0	26.0	38.0	2.0	40.0
68-69	28.3356068679457	34.0	26.0	37.5	2.0	40.0
70-71	28.01742501108378	34.0	26.0	37.0	2.0	39.0
72-73	27.557511145477378	34.0	25.0	36.0	2.0	39.0
74-75	27.116178890407696	33.0	24.0	36.0	2.0	39.0
76-77	26.698339006887405	33.0	23.5	35.5	2.0	38.0
78-79	26.242250500011203	33.0	22.0	35.0	2.0	37.0
80-81	25.79014676545014	32.0	20.0	35.0	2.0	37.0
82-83	25.31373018995462	32.0	18.5	35.0	2.0	36.0
84-85	24.913037478360742	32.0	17.5	35.0	2.0	36.0
86-87	24.458382559132655	31.5	9.5	34.5	2.0	35.0
88-89	23.932411476530035	31.0	4.5	34.0	2.0	35.0
90-91	23.443508165407763	31.0	2.0	34.0	2.0	35.0
92-93	22.926677551418685	31.0	2.0	34.0	2.0	35.0
94-95	22.311021151800027	30.0	2.0	34.0	2.0	35.0
96-97	21.6813667076925	30.0	2.0	34.0	2.0	35.0
98	21.294415900966154	29.0	2.0	34.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	180974.0
3	30144.0
4	22053.0
5	17776.0
6	16164.0
7	16329.0
8	18784.0
9	21649.0
10	23881.0
11	25502.0
12	26525.0
13	26643.0
14	25443.0
15	26279.0
16	28132.0
17	30406.0
18	31343.0
19	31224.0
20	32348.0
21	34577.0
22	37197.0
23	41942.0
24	47844.0
25	54977.0
26	64262.0
27	73303.0
28	85448.0
29	98750.0
30	115105.0
31	135172.0
32	161661.0
33	195895.0
34	243659.0
35	310806.0
36	400135.0
37	460847.0
38	299409.0
39	32830.0
40	3.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.242796305640056	18.09468705117502	12.837718553919364	24.82479808926556
2	15.944965722030535	19.543790769192356	39.53926822784189	24.97197528093522
3	19.554942242810565	23.48026359838809	28.795723732366156	28.169070426435187
4	13.115156859023624	15.434289032762502	36.87606263296654	34.574491475247335
5	14.377160327625042	36.848482922648245	34.38462775904142	14.389728990685288
6	33.642597478354304	35.01983060219832	17.320333665947626	14.017238253499755
7	29.423840176572885	31.302423621730043	20.91171639398676	18.36201980771031
8	26.45918425819966	34.67555117804218	20.018717273848072	18.84654728991009
9	26.571663355950964	15.9220416511957	19.668629647352756	37.83766534550058
10-11	25.530848172820285	25.506453912789667	27.802393445659447	21.1603044687306
12-13	26.135732150774544	22.757547932238314	27.58902451097614	23.517695406011
14-15	23.19842611953963	24.42903078163997	25.211333108009686	27.161209990810708
16-17	23.347355679731102	27.316030989957607	25.945501348644402	23.39111198166689
18-19	23.21612382159713	26.768304837898445	27.30664048505967	22.708930855444756
20-21	24.100246478648334	26.081188207348653	27.029318397470064	22.78924691653295
22-23	23.64560518032381	25.973951303002718	26.761017075238602	23.619426441434868
24-25	23.403188811315843	26.044390628676595	27.007837630789204	23.544582929218357
26-27	23.332767967457634	26.327395099200423	26.882182246044863	23.45765468729708
28-29	23.33045969059737	26.414112494953386	26.852765189330484	23.402662625118758
30-31	23.66946536245021	26.21028645574657	26.669695812116544	23.450552369686672
32-33	23.38783259605935	26.309176734674423	26.6116709072215	23.691319762044728
34-35	23.678469515600106	26.198590466150296	26.713646384718153	23.409293633531444
36-37	23.708027115841094	26.07175290654159	26.641442186375073	23.57877779124224
38-39	23.602009707678107	26.17168585335769	26.618908226788367	23.60739621217584
40-41	23.653470578577977	25.996974744503575	26.770105094423087	23.57944958249536
42-43	23.567706942326254	25.848314012178257	26.74093727507972	23.843041770415766
44-45	23.454372937892956	26.134327089320553	26.680192251853292	23.7311077209332
46-47	23.549932708008864	25.990330060416827	26.572881761898138	23.886855469676167
48-49	23.34574164446693	25.98641517042407	26.72916244540602	23.938680739702985
50-51	23.33503957945186	26.139040311310417	26.76247274218431	23.76344736705341
52-53	23.38345528303548	25.970528054225277	26.836319175314365	23.80969748742487
54-55	23.154546528255548	25.989069942084285	26.9093444031074	23.94703912655277
56-57	23.21478166619903	25.996185799151988	26.892693911094085	23.896338623554897
58-59	23.115255933628916	25.83985631636375	26.991264998731147	24.053622751276187
60-61	23.283332436034716	25.769561750809068	27.05883743712144	23.888268376034777
62-63	23.074538437889238	25.74839683412386	27.152125500408612	24.024939227578283
64-65	23.011296286164985	25.666254068988152	27.053214842574608	24.269234802272248
66-67	23.00647259613206	25.84542604821658	26.876649605719116	24.27145174993224
68-69	23.012755439191345	25.626449805401364	26.9628057978544	24.397988957552887
70-71	22.891388060445152	25.419706600422803	27.103316226662205	24.585589112469837
72-73	22.97171904110601	25.444635795845088	27.06219328560129	24.52145187744761
74-75	22.873511331273015	25.431864071402586	27.130046502308037	24.56457809501636
76-77	22.848009718160814	25.357913168415298	27.18208914272183	24.611987970702067
78-79	22.79661038657566	25.346220611770974	27.19311478013422	24.664054221519145
80-81	22.927925235134705	25.30855024936804	27.12120823939332	24.642316276103937
82-83	22.873569058719564	25.245340309650206	27.251287599858642	24.62980303177159
84-85	22.966577659791444	25.1399043758448	27.211637721285463	24.681880243078293
86-87	22.818297785865905	25.069360680662516	27.32564626109785	24.78669527237373
88-89	22.89917829726428	24.959450165167702	27.32272287033463	24.818648667233383
90-91	22.878054140781064	24.967511033438086	27.398952552871258	24.755482272909596
92-93	22.97279141412517	24.75724562703398	27.413702752454444	24.856260206386406
94-95	22.905510991766157	24.71176423785672	27.46057756891462	24.922147201462504
96-97	23.038113017625484	24.558708155273695	27.480423403327425	24.922755423773395
98	23.07291602916106	24.408254842457005	27.426067614923937	25.092761513458
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	99.0
1	80.5
2	103.5
3	156.0
4	166.0
5	199.0
6	294.0
7	546.5
8	801.0
9	944.5
10	1064.0
11	1123.5
12	1254.5
13	1434.5
14	1631.5
15	1786.5
16	1887.0
17	1995.5
18	2015.0
19	2001.5
20	2016.0
21	2119.0
22	2296.0
23	2679.5
24	3243.5
25	4056.0
26	5356.0
27	6941.0
28	9264.0
29	13139.0
30	18821.0
31	26341.0
32	35704.0
33	45629.0
34	55973.0
35	66634.5
36	77480.0
37	88188.5
38	97791.0
39	106036.0
40	114373.0
41	122711.5
42	129893.5
43	136763.5
44	142662.0
45	148197.5
46	153953.5
47	158276.5
48	159884.0
49	158679.0
50	157366.0
51	155372.5
52	150762.0
53	145972.0
54	139749.0
55	130765.0
56	119814.5
57	108676.0
58	96785.5
59	82316.5
60	66952.0
61	52568.0
62	40924.0
63	31621.0
64	23683.5
65	17615.5
66	12976.0
67	9507.0
68	6790.5
69	4789.5
70	3511.0
71	2535.5
72	2020.0
73	1606.0
74	1185.0
75	848.5
76	572.0
77	344.5
78	203.0
79	114.0
80	65.0
81	42.0
82	25.5
83	12.5
84	7.5
85	7.0
86	8.5
87	7.5
88	7.0
89	6.5
90	3.0
91	1.0
92	2.0
93	3.5
94	3.5
95	1.5
96	0.0
97	1.0
98	1.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.38216712273512865
2	0.024224057211890436
3	0.018862995369914686
4	0.01611155093249856
5	0.022181747938756817
6	0.02320290257532363
7	0.015062030889360449
8	0.009502411201385593
9	0.0
10-11	4.254810985695042E-5
12-13	0.005219234809119251
14-15	0.0034463968984129838
16-17	0.00608437970954391
18-19	0.03739978856425942
20-21	0.07952241732264033
22-23	0.09868324946155367
24-25	0.06495678104827764
26-27	0.07455847117266279
28-29	0.05725557316416961
30-31	0.05318513732118802
32-33	0.020564919764192703
34-35	0.338087280923328
36-37	0.517555208299945
38-39	0.4723407502252922
40-41	0.48099219922953884
42-43	0.42972172685191357
44-45	0.40555440045316576
46-47	0.4036539182128886
48-49	0.39514429624149855
50-51	0.3918255436726564
52-53	0.45140708017567266
54-55	0.4517049169446713
56-57	0.421396480023237
58-59	0.4077243540558702
60-61	0.42219071140723335
62-63	0.40181016678575404
64-65	0.4523998694056681
66-67	0.47165998046758106
68-69	0.4142767629738406
70-71	0.3728916347863135
72-73	0.359857730466801
74-75	0.34171805296445446
76-77	0.340356513449032
78-79	0.33987430153731996
80-81	0.3557305638106768
82-83	0.3515183009348387
84-85	0.35163176256112394
86-87	0.3376334344181872
88-89	0.3796567842535686
90-91	0.3599570093898006
92-93	0.34895123164013603
94-95	0.35574474651396243
96-97	0.3560709486895324
98	0.35116373335269746
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
98	3525421.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.01288704718946
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.94686153933152	33.354167879011825
2	11.73874456399355	11.037445445256761
3	5.486667445032284	7.738322305763829
4	3.1917977518891423	6.0022250874814995
5	2.1430189032044	5.037475281817015
6	1.4316753645637676	4.038431532148811
7	1.0175600867442463	3.348690619528489
8	0.7370758928815979	2.7721652557799104
9	0.5792404979702976	2.450859129381184
>10	2.6376209830073893	20.383087786483575
>50	0.07010704426292266	2.1896450294256926
>100	0.01926562343206054	1.4651395290730032
>500	1.8215184581532782E-4	0.050810005803293506
>1k	1.8215184091134395E-4	0.1315351130450324
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.1201947228430305E-4
2	0.0	0.0	0.0	0.0	3.1201947228430305E-4
3	0.0	0.0	0.0	0.0	3.1201947228430305E-4
4	0.0	0.0	0.0	0.0	3.1201947228430305E-4
5	0.0	0.0	0.0	0.0	3.1201947228430305E-4
6	0.0	0.0	0.0	0.0	5.389427248547053E-4
7	0.0	0.0	0.0	0.0	5.673081314260056E-4
8	0.0	0.0	0.0	0.0	5.956735379973059E-4
9	0.0	0.0	0.0	0.0	5.956735379973059E-4
10-11	0.0	0.0	0.0	2.8365406571300278E-5	6.524043511399064E-4
12-13	0.0	0.0	0.0	2.8365406571300278E-5	9.786065267098597E-4
14-15	2.8365406571300278E-5	0.0	0.0	2.8365406571300278E-5	0.0014182703285650139
16-17	2.8365406571300278E-5	0.0	0.0	2.8365406571300278E-5	0.001716107097563667
18-19	2.8365406571300278E-5	0.0	0.0	2.8365406571300278E-5	0.0017870206139919175
20-21	2.8365406571300278E-5	0.0	0.0	2.8365406571300278E-5	0.0019713957567053693
22-23	2.8365406571300278E-5	0.0	0.0	1.843751427134518E-4	0.0020848573829905705
24-25	2.8365406571300278E-5	0.0	0.0	4.963946149977549E-4	0.002340146042132273
26-27	2.8365406571300278E-5	0.0	0.0	0.001318991405565463	0.002467790371703124
28-29	2.8365406571300278E-5	0.0	0.0	0.0062687548522573616	0.0024961557782744244
30-31	2.8365406571300278E-5	0.0	0.0	0.02608199134231061	0.0025670692947026754
32-33	2.8365406571300278E-5	0.0	0.0	0.06980726557196999	0.0026663482177022263
34-35	2.8365406571300278E-5	2.8365406571300278E-5	0.0	0.13256572761097185	0.0028932714702726283
36-37	2.8365406571300278E-5	2.8365406571300278E-5	0.0	0.23611364429950354	0.0028932714702726283
38-39	2.8365406571300278E-5	2.8365406571300278E-5	0.0	0.4067457475291604	0.002964184986700879
40-41	2.8365406571300278E-5	2.8365406571300278E-5	0.0	0.607785566603251	0.00303509850312913
42-43	2.8365406571300278E-5	2.8365406571300278E-5	0.0	0.8129809177400373	0.003120194722843031
44-45	2.8365406571300278E-5	2.8365406571300278E-5	0.0	1.0636318329073322	0.003262021755699532
46-47	2.8365406571300278E-5	2.8365406571300278E-5	0.0	1.3545332599993023	0.0034038487885560335
48-49	2.8365406571300278E-5	2.8365406571300278E-5	0.0	1.671147928148156	0.003574041227983835
50-51	5.6730813142600556E-5	2.8365406571300278E-5	0.0	2.011220787531475	0.0036307720411264356
52-53	5.6730813142600556E-5	2.8365406571300278E-5	0.0	2.3576900460966224	0.0039569742166963885
54-55	5.6730813142600556E-5	2.8365406571300278E-5	0.0	2.7538696796779734	0.004198080172552441
56-57	5.6730813142600556E-5	2.8365406571300278E-5	0.0	3.203801758711938	0.004254810985695042
58-59	5.6730813142600556E-5	2.8365406571300278E-5	0.0	3.6998134407209804	0.004339907205408942
60-61	5.6730813142600556E-5	2.8365406571300278E-5	0.0	4.2230417303351855	0.0044250034251228434
62-63	5.6730813142600556E-5	2.8365406571300278E-5	0.0	4.758552240994764	0.004481734238265444
64-65	5.6730813142600556E-5	2.8365406571300278E-5	0.0	5.32478248697106	0.004552647754693695
66-67	7.09135164282507E-5	2.8365406571300278E-5	0.0	5.961415672057323	0.004623561271121946
68-69	8.509621971390084E-5	2.8365406571300278E-5	0.0	6.658382076920742	0.004680292084264546
70-71	8.509621971390084E-5	2.8365406571300278E-5	0.0	7.412221121959618	0.004850484523692348
72-73	8.509621971390084E-5	2.8365406571300278E-5	0.0	8.17184670993904	0.005431975358404003
74-75	8.509621971390084E-5	2.8365406571300278E-5	0.0	8.94359850922769	0.005602167797831805
76-77	8.509621971390084E-5	2.8365406571300278E-5	0.0	9.771939294626089	0.005630533204403106
78-79	8.509621971390084E-5	5.6730813142600556E-5	0.0	10.682837595850255	0.0056730813142600554
80-81	8.509621971390084E-5	5.6730813142600556E-5	0.0	11.636255641524798	0.005800725643830907
82-83	8.509621971390084E-5	5.6730813142600556E-5	0.0	12.61240856056624	0.00612692781940086
84-85	8.509621971390084E-5	5.6730813142600556E-5	0.0	13.579385270581868	0.00616947592925781
86	8.509621971390084E-5	5.6730813142600556E-5	0.0	14.324587049319783	0.006183658632543461
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	5825	0.0	42.350407	1
TCAACGC	6765	0.0	35.92969	4
ATCAACG	6930	0.0	35.074223	3
GGTATCA	3230	0.0	34.767708	1
CAACGCA	7020	0.0	34.493896	5
TATCAAC	7205	0.0	33.799644	2
AACGCAG	7390	0.0	32.63719	6
ACGCAGA	8840	0.0	26.762447	7
GTACATG	10560	0.0	26.368183	1
CGCAGAG	8890	0.0	25.73262	8
TACATGG	10835	0.0	25.227194	2
ACATGGG	11595	0.0	22.861383	3
GCAGAGT	10545	0.0	20.520155	9
CATGGGG	7615	0.0	19.99464	4
GAGTACA	8060	0.0	19.7574	1
CGCCGTA	385	1.8189894E-12	19.12351	92
GTGTAGC	2075	0.0	18.63159	1
AGTACAT	7885	0.0	18.612291	2
AGAGTAC	8595	0.0	17.629066	10-11
GAGTACT	4065	0.0	16.358158	12-13
>>END_MODULE