FastQCFastQC Report
Mon 15 Aug 2016
SRR2920880_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920880_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3525421
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA56300.040.6389581
GGTATCA31450.039.4753271
TCAACGC66650.033.338214
ATCAACG67650.032.7752233
CAACGCA69650.032.1694345
TATCAAC71900.031.4290642
AACGCAG73150.030.4359766
GTACATG107400.025.422221
ACGCAGA88050.024.6385737
TACATGG110100.024.4052072
CGCAGAG88100.024.2439618
ACATGGG118200.022.0120073
CATGGGG74850.019.9809134
GCAGAGT106450.019.2178489
GAGTACA84000.018.4039041
AGTACAT81600.017.6281152
GTATAGG8200.017.4025651
AGAGTAC87150.017.39825210-11
GTGTAGC19500.017.0753381
CAGAGTA96850.015.65573410-11
Produced by FastQC (version 0.11.5)