##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920880_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3525421
Sequences flagged as poor quality	0
Sequence length	101
%GC	47
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.228782888625215	31.0	30.0	34.0	26.0	34.0
2	30.246823287204563	31.0	30.0	34.0	26.0	34.0
3	30.385627702336826	31.0	30.0	34.0	27.0	34.0
4	34.02412704752142	35.0	35.0	37.0	30.0	37.0
5	33.55386377967341	35.0	35.0	37.0	28.0	37.0
6	33.48664400648887	35.0	35.0	37.0	28.0	37.0
7	33.304743178190634	35.0	33.0	37.0	27.0	37.0
8	33.239502459422575	35.0	33.0	37.0	27.0	37.0
9	34.5673313910594	38.0	34.0	39.0	27.0	39.0
10-11	34.38100867953076	37.5	34.0	39.0	27.0	39.0
12-13	34.20295334940139	37.0	34.0	39.0	26.0	39.0
14-15	35.110581970210085	38.0	34.0	40.0	26.0	41.0
16-17	34.995084275041194	38.0	34.0	40.0	25.5	41.0
18-19	34.906958346251415	38.5	34.0	40.0	25.0	41.0
20-21	34.79523991602705	38.0	34.0	40.0	25.0	41.0
22-23	34.64770434509808	38.0	34.0	40.0	24.5	41.0
24-25	34.476176462328894	38.0	33.0	40.0	24.0	41.0
26-27	34.29749269661694	38.0	33.0	40.0	23.0	41.0
28-29	34.24618222901605	38.0	33.0	40.0	23.5	41.0
30-31	34.23672662073551	38.0	33.0	40.0	23.0	41.0
32-33	34.09419414021758	38.0	33.0	40.0	22.0	41.0
34-35	33.931846863112234	38.0	33.0	40.0	21.0	41.0
36-37	33.748928851334355	38.0	33.0	40.0	19.0	41.0
38-39	33.58062781721672	38.0	32.5	40.0	18.5	41.0
40-41	33.35906080436918	38.0	32.0	40.0	17.0	41.0
42-43	33.3957097606215	38.0	32.0	40.0	17.0	41.0
44-45	33.26202969801337	38.0	32.0	40.0	15.5	41.0
46-47	33.031796486150164	38.0	32.0	40.0	14.5	41.0
48-49	32.78498894174625	37.5	31.5	40.0	11.5	41.0
50-51	32.53640699933426	37.0	31.0	40.0	9.0	41.0
52-53	32.290683013461376	37.0	31.0	40.0	8.0	41.0
54-55	32.023129436172304	37.0	31.0	40.0	7.5	41.0
56-57	31.7067964365107	36.0	30.0	39.5	6.5	41.0
58-59	31.388949716927424	36.0	30.0	39.0	2.0	41.0
60-61	31.067996276189426	35.5	29.0	39.0	2.0	40.0
62-63	30.754517261909996	35.0	29.0	39.0	2.0	40.0
64-65	30.361222248349915	35.0	28.5	38.5	2.0	40.0
66-67	29.932319005304613	34.5	28.0	38.0	2.0	40.0
68-69	29.492397645557794	34.0	28.0	37.5	2.0	40.0
70-71	29.07454244471795	34.0	27.5	37.0	2.0	39.0
72-73	28.630007309765276	34.0	26.0	36.0	2.0	39.0
74-75	28.18095810401084	33.5	26.0	36.0	2.0	39.0
76-77	26.717416870212098	31.5	25.0	34.5	2.0	36.5
78-79	27.56145365333672	33.0	26.0	35.0	2.0	37.0
80-81	27.580834175549533	33.0	26.0	35.0	2.0	37.0
82-83	27.235212191678666	33.0	26.0	35.0	2.0	36.0
84-85	26.90793326527527	33.0	25.5	35.0	2.0	36.0
86-87	26.36015159607888	33.0	24.0	35.0	2.0	35.5
88-89	25.997555894742785	32.0	23.5	35.0	2.0	35.0
90-91	25.627474704439557	32.0	21.5	35.0	2.0	35.0
92-93	25.322417804852243	32.0	20.0	34.0	2.0	35.0
94-95	24.951977933982917	32.0	18.5	34.0	2.0	35.0
96-97	24.42913087543303	32.0	11.5	34.0	2.0	35.0
98-99	23.83710371045047	32.0	2.0	34.0	2.0	35.0
100-101	21.651878456502075	28.0	2.0	33.0	2.0	34.5
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	85614.0
3	38536.0
4	20103.0
5	13922.0
6	11861.0
7	11731.0
8	13238.0
9	15265.0
10	17405.0
11	19729.0
12	21820.0
13	23990.0
14	25824.0
15	27533.0
16	28872.0
17	29644.0
18	29540.0
19	28153.0
20	28508.0
21	29644.0
22	32315.0
23	36523.0
24	42720.0
25	50112.0
26	59935.0
27	70463.0
28	83443.0
29	98338.0
30	115664.0
31	138037.0
32	165069.0
33	201371.0
34	252730.0
35	327693.0
36	440342.0
37	534420.0
38	330552.0
39	24762.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.237180525596315	19.503391906601074	13.535625676747179	24.723801891055427
2	15.993947956853946	20.657703009087424	38.49665047096503	24.8516985630936
3	19.305106382978725	24.394808510638295	29.172141843971634	27.127943262411346
4	13.454216989150428	16.437735536458575	36.460221076605166	33.64782639778583
5	14.250863074047073	37.16503223939703	34.4563555439565	14.127749142599406
6	33.50217195870411	35.6402834308145	17.050741884756988	13.806802725724406
7	29.218675214707023	31.546132007004697	20.91512699208492	18.32006578620336
8	26.331420184370042	35.14476135244078	19.92336990648584	18.600448556703345
9	26.2724608304251	15.965915837182735	19.689006726958	38.07261660543417
10-11	25.476511946325843	25.731595405402373	27.851052842245966	20.940839806025817
12-13	26.05808095257635	22.861017443936593	27.71241308523131	23.368488518255738
14-15	23.077342490292217	24.555432750932727	25.237689420293396	27.129535338481663
16-17	23.306179332777955	27.53177230944775	25.958533854273846	23.203514503500454
18-19	23.19802048918035	26.92145501936944	27.289029387639584	22.591495103810626
20-21	24.02571357672481	26.197171188751383	27.036711608721642	22.740403625802166
22-23	23.612671115286787	26.129267564613983	26.77275657126278	23.485304748836448
24-25	23.338491194928572	26.24808992616273	26.983482544252407	23.42993633465629
26-27	23.320352525438885	26.44105417027376	26.922214495550854	23.3163788087365
28-29	23.301521044988625	26.472879628099143	26.90620116158718	23.319398165325055
30-31	23.610839210639355	26.29804339474785	26.75981551245626	23.331301882156538
32-33	23.394816641068324	26.328236080920075	26.63639508023824	23.640552197773356
34-35	23.61072928289967	26.31472555606718	26.787885184280462	23.28665997675269
36-37	23.68493999524479	26.192448746706674	26.656592578805714	23.46601867924282
38-39	23.55576161713953	26.29928748910776	26.668396562491754	23.476554331260957
40-41	23.6337298956091	26.08741109835295	26.803362480391062	23.475496525646886
42-43	23.57123100163831	25.95234008751835	26.793480804828402	23.682948106014937
44-45	23.42604153263395	26.170984299392693	26.72937849155968	23.67359567641368
46-47	23.48275077843212	26.084537863077383	26.613504249082386	23.819207109408108
48-49	23.272663109911342	26.04989953542047	26.801844928531573	23.87559242613662
50-51	23.269463372896197	26.18715187807642	26.747565495894705	23.79581925313268
52-53	23.35096882190672	26.026162893364273	26.762961417469473	23.859906867259532
54-55	23.145145527370584	26.07251560067824	26.81781908518196	23.964519786769216
56-57	23.182351542026044	26.1233924360226	26.694898246180376	23.999357775770985
58-59	23.022947279917897	26.0020003982642	26.826725003106123	24.14832731871178
60-61	23.257589825220272	25.978154059290937	26.79040803214743	23.97384808334136
62-63	22.946046373217467	25.97216767253222	26.86288668874564	24.218899265504675
64-65	22.911722776495814	25.89121221882145	26.73292702835837	24.46413797632436
66-67	22.85416130174182	26.06324120205531	26.561276486766992	24.521321009435884
68-69	22.892864529443237	25.857281503921502	26.646152675593854	24.603701291041407
70-71	22.724909520116515	25.655389562480664	26.697966920954634	24.921733996448186
72-73	22.83670483938937	25.633212680854566	26.61891174396513	24.911170735790936
74-75	22.67812850694867	25.68719515362937	26.604576730369107	25.030099609052847
76-77	22.591602506429293	25.560153279366315	26.76325014587473	25.084994068329657
78-79	22.54107522828009	25.583693141824703	26.75574218583108	25.11948944406412
80-81	22.623154851084486	25.60492627915336	26.635409553481836	25.13650931628032
82-83	22.53644750739078	25.51311332961324	26.755453402708255	25.19498576028773
84-85	22.508928472643227	25.390422980806303	26.62134509933307	25.4793034472174
86-87	22.340091815706504	25.386158364176513	26.71955369671694	25.554196123400036
88-89	22.385151072992734	25.35242182611992	26.606663332830976	25.65576376805637
90-91	22.249061296045696	25.359995312879512	26.646919896314525	25.744023494760267
92-93	22.26424885510438	25.123427832293004	26.72525693256787	25.887066380034753
94-95	22.045239062711637	25.131372269533873	26.774328743051285	26.049059924703204
96-97	22.066101261241023	25.027373882989558	26.786989774932785	26.119535080836638
98-99	22.003134485008367	24.945763481122174	26.73478852863586	26.316313505233595
100-101	22.07407615431246	24.808729171967727	26.84420933968411	26.2729853340357
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	104.0
1	72.5
2	68.0
3	109.0
4	101.0
5	96.0
6	172.0
7	337.5
8	521.0
9	557.0
10	481.5
11	420.0
12	411.5
13	485.5
14	565.5
15	623.0
16	677.0
17	742.5
18	885.0
19	1048.5
20	1182.0
21	1358.0
22	1683.0
23	2147.5
24	2656.0
25	3298.5
26	4393.5
27	5796.0
28	7534.0
29	10607.0
30	15084.0
31	21265.0
32	29487.0
33	38275.0
34	47689.5
35	57947.0
36	68363.0
37	78856.5
38	88675.0
39	97510.5
40	105051.0
41	113401.5
42	121735.5
43	129137.0
44	136529.5
45	142255.0
46	147532.5
47	152336.0
48	156558.5
49	158845.0
50	157945.0
51	154671.5
52	149981.0
53	145486.5
54	139381.0
55	130737.0
56	120658.5
57	108546.5
58	96726.0
59	83649.5
60	67548.5
61	53047.0
62	41406.0
63	31414.5
64	23610.0
65	17908.5
66	13130.5
67	9428.0
68	6793.0
69	4875.5
70	3466.0
71	2528.5
72	1927.5
73	1557.0
74	1220.5
75	845.0
76	575.0
77	365.5
78	193.5
79	91.5
80	45.5
81	22.0
82	8.0
83	4.5
84	4.5
85	3.5
86	1.0
87	0.5
88	1.0
89	1.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.19149485976284816
2	0.0
3	0.011941836166517417
4	0.013870683813365836
5	0.006552408917970364
6	0.10537748541238053
7	0.13972799277022518
8	0.1233895185851562
9	0.1406640511870781
10-11	0.11415657874619797
12-13	0.11174551918763745
14-15	0.11400056901005583
16-17	0.06541062755341845
18-19	0.07750847345607802
20-21	0.03660555718026301
22-23	0.08241568879291296
24-25	0.056645716922886655
26-27	0.06441783832342293
28-29	0.038676231859967936
30-31	0.024025499365891337
32-33	0.03131540885471551
34-35	0.020196169478765798
36-37	0.02459280749731734
38-39	0.031882716986141514
40-41	0.024961557782744243
42-43	0.012650971330799924
44-45	0.02664929947373661
46-47	0.03374065111656168
48-49	0.008452891158247483
50-51	0.014877655746646998
52-53	0.006736784060683816
54-55	0.011743278320518316
56-57	0.0048930326335492975
58-59	0.0034889450082699345
60-61	0.02225266145518507
62-63	0.009856978783526846
64-65	0.006708418654112516
66-67	0.038959885925680934
68-69	0.08820223173345822
70-71	0.021061314379190458
72-73	0.043285610427804225
74-75	0.08367794938533582
76-77	0.24692086420316892
78-79	0.08025991789349414
80-81	0.016168281745641156
82-83	0.011913470759946117
84-85	0.013104817835940728
86-87	0.2622949145648137
88-89	0.6225923088334698
90-91	0.26671991798993655
92-93	0.35278056152726156
94-95	0.0010920681529950607
96-97	0.004623561271121945
98-99	0.0034322141951273334
100-101	0.005219234809119251
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3525421.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.19670860825068
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.04783150781797	30.07490180574371
2	12.644820797861792	11.177189204132908
3	6.170915543618445	8.182024683822878
4	3.5709181991290815	6.312913244432291
5	2.2916699454916767	5.064213440359068
6	1.5750706256538067	4.176776248766183
7	1.154436761198702	3.57156135989462
8	0.8205045635114168	2.90108808842032
9	0.6355865428592652	2.5281749907068733
>10	2.981562748541343	21.6659876291243
>50	0.08320460888621778	2.4579072254699863
>100	0.02296333626016761	1.6521065913489028
>500	2.5740958624108275E-4	0.07340787581553349
>1k	2.5740958387608014E-4	0.1617476119624009
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.7019243942780168E-4
2	0.0	0.0	0.0	5.6730813142600556E-5	1.7019243942780168E-4
3	0.0	0.0	0.0	5.6730813142600556E-5	1.7019243942780168E-4
4	0.0	0.0	0.0	5.6730813142600556E-5	1.7019243942780168E-4
5	0.0	0.0	0.0	5.6730813142600556E-5	1.9855784599910194E-4
6	0.0	0.0	0.0	5.6730813142600556E-5	2.836540657130028E-4
7	0.0	0.0	0.0	5.6730813142600556E-5	3.1201947228430305E-4
8	0.0	0.0	0.0	5.6730813142600556E-5	3.4038487885560336E-4
9	0.0	0.0	0.0	5.6730813142600556E-5	4.254810985695042E-4
10-11	0.0	0.0	0.0	5.6730813142600556E-5	4.5384650514080445E-4
12-13	0.0	0.0	0.0	5.6730813142600556E-5	7.658659774251076E-4
14-15	0.0	0.0	0.0	5.6730813142600556E-5	0.0010778854497094106
16-17	0.0	0.0	0.0	5.6730813142600556E-5	0.001517549251564565
18-19	0.0	0.0	0.0	5.6730813142600556E-5	0.001730289800849317
20-21	0.0	0.0	0.0	9.927892299955098E-5	0.001829568723848868
22-23	0.0	0.0	0.0	2.552886591417025E-4	0.002113222789561871
24-25	0.0	0.0	0.0	5.105773182834051E-4	0.0022975979322753227
26-27	0.0	0.0	0.0	0.0012764432957085125	0.0025245211848457247
28-29	0.0	0.0	0.0	0.006226206742400411	0.0025812519979883253
30-31	0.0	0.0	0.0	0.025784154573311954	0.002623800107845276
32-33	0.0	0.0	0.0	0.06962289042925654	0.0027372617341304773
34-35	0.0	0.0	0.0	0.1326082757208288	0.002950002283415229
36-37	0.0	0.0	0.0	0.23632638484878826	0.002964184986700879
38-39	0.0	0.0	0.0	0.4077243540558702	0.00307764661298608
40-41	0.0	0.0	0.0	0.609047827195674	0.003106012019557381
42-43	0.0	0.0	0.0	0.8165407762647354	0.003162742832699981
44-45	0.0	0.0	0.0	1.067971740112741	0.0033045698655564826
46-47	0.0	2.8365406571300278E-5	0.0	1.3585327823258555	0.003559858524698185
48-49	0.0	2.8365406571300278E-5	0.0	1.6746936039695686	0.0038009644805542375
50-51	0.0	2.8365406571300278E-5	0.0	2.012681605969897	0.003971156919982039
52-53	0.0	2.8365406571300278E-5	0.0	2.3558746600760587	0.004212262875838092
54-55	0.0	2.8365406571300278E-5	0.0	2.7483242426932843	0.004368272611980243
56-57	0.0	2.8365406571300278E-5	0.0	3.1937320393791264	0.004453368831694143
58-59	0.0	2.8365406571300278E-5	0.0	3.6849074195677622	0.004538465051408045
60-61	0.0	2.8365406571300278E-5	0.0	4.204221283075128	0.004609378567836295
62-63	0.0	2.8365406571300278E-5	0.0	4.735547896265439	0.004793753710549747
64-65	0.0	2.8365406571300278E-5	0.0	5.296275253366903	0.004850484523692348
66-67	0.0	2.8365406571300278E-5	0.0	5.923803142943779	0.004921398040120598
68-69	0.0	2.8365406571300278E-5	0.0	6.610614732254673	0.004963946149977549
70-71	0.0	2.8365406571300278E-5	0.0	7.3546960774330215	0.005190869402547951
72-73	1.4182703285650139E-5	2.8365406571300278E-5	0.0	8.104819254211057	0.005701446720831356
74-75	2.8365406571300278E-5	2.8365406571300278E-5	0.0	8.875564081566427	0.005843273753687858
76-77	2.8365406571300278E-5	2.8365406571300278E-5	0.0	9.700004623561272	0.005914187270116108
78-79	2.8365406571300278E-5	2.8365406571300278E-5	0.0	10.606747392722742	0.0059567353799730586
80-81	2.8365406571300278E-5	2.8365406571300278E-5	0.0	11.557101974487587	0.00607019700625826
82-83	2.8365406571300278E-5	2.8365406571300278E-5	0.0	12.52758181221477	0.006353851071971262
84-85	2.8365406571300278E-5	2.8365406571300278E-5	0.0	13.491792895089691	0.006353851071971262
86-87	2.8365406571300278E-5	2.8365406571300278E-5	0.0	14.495743912571008	0.006524043511399064
88-89	2.8365406571300278E-5	2.8365406571300278E-5	0.0	15.554695453394077	0.0067367840606838165
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	5630	0.0	40.638958	1
GGTATCA	3145	0.0	39.475327	1
TCAACGC	6665	0.0	33.33821	4
ATCAACG	6765	0.0	32.775223	3
CAACGCA	6965	0.0	32.169434	5
TATCAAC	7190	0.0	31.429064	2
AACGCAG	7315	0.0	30.435976	6
GTACATG	10740	0.0	25.42222	1
ACGCAGA	8805	0.0	24.638573	7
TACATGG	11010	0.0	24.405207	2
CGCAGAG	8810	0.0	24.243961	8
ACATGGG	11820	0.0	22.012007	3
CATGGGG	7485	0.0	19.980913	4
GCAGAGT	10645	0.0	19.217848	9
GAGTACA	8400	0.0	18.403904	1
AGTACAT	8160	0.0	17.628115	2
GTATAGG	820	0.0	17.402565	1
AGAGTAC	8715	0.0	17.398252	10-11
GTGTAGC	1950	0.0	17.075338	1
CAGAGTA	9685	0.0	15.655734	10-11
>>END_MODULE