FastQCFastQC Report
Mon 15 Aug 2016
SRR2920879_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920879_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4083011
Sequences flagged as poor quality0
Sequence length98
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA58600.042.6454051
GGTATCA34000.035.193661
TCAACGC70150.034.8428044
CAACGCA71550.034.0328645
ATCAACG71500.033.9925233
TATCAAC72600.033.4787182
AACGCAG75850.032.4022336
ACGCAGA92300.025.9790427
GTACATG100450.025.1073381
CGCAGAG96350.024.6006188
TACATGG103650.023.9805722
ACATGGG107200.022.2444743
TAAGGCG9200.021.4335565
GTATAGG7850.021.1058521
GCAGAGT110500.020.4956289
CATGGGG70900.019.274524
GAGTACA72550.018.7134841
AGAGTAC88350.018.18804410-11
AGTACAT74100.017.143172
CGTATCA5950.017.0131692
Produced by FastQC (version 0.11.5)