##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920879_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4083011
Sequences flagged as poor quality	0
Sequence length	98
%GC	47
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.837229926639925	31.0	30.0	33.0	23.0	34.0
2	28.861334686583014	31.0	30.0	34.0	22.0	34.0
3	28.94561366599306	31.0	30.0	34.0	22.0	34.0
4	32.43629639988724	35.0	33.0	37.0	25.0	37.0
5	32.42097290455499	35.0	33.0	37.0	25.0	37.0
6	32.55362402893355	35.0	33.0	37.0	25.0	37.0
7	32.40064991252779	35.0	33.0	37.0	25.0	37.0
8	32.34788762508845	35.0	33.0	37.0	24.0	37.0
9	33.63965392206879	37.0	33.0	39.0	24.0	39.0
10-11	33.461407280068556	37.0	33.0	39.0	23.0	39.0
12-13	33.30926527016459	37.0	33.0	39.0	22.0	39.0
14-15	34.11120690098557	38.0	33.0	40.0	20.5	41.0
16-17	34.01843737379106	38.0	33.0	40.0	19.0	41.0
18-19	33.921576503222745	38.0	33.0	40.0	18.5	41.0
20-21	33.834601596713796	38.0	33.0	40.0	18.0	41.0
22-23	33.633307257805576	38.0	32.0	40.0	18.0	41.0
24-25	33.46146459071504	38.0	32.0	40.0	18.0	41.0
26-27	33.51415168854554	38.0	32.0	40.0	17.0	41.0
28-29	33.365085472461374	38.0	32.0	40.0	16.0	41.0
30-31	33.14134360647081	38.0	32.0	40.0	15.5	41.0
32-33	32.909083394583064	38.0	31.5	40.0	12.5	41.0
34-35	32.597995817302476	37.5	31.0	40.0	9.0	41.0
36-37	32.31414757393502	37.0	30.5	40.0	9.0	41.0
38-39	32.13583015083721	37.0	30.0	40.0	8.0	41.0
40-41	31.96151724793296	37.0	30.0	40.0	7.5	41.0
42-43	31.749346132057937	37.0	30.0	40.0	4.0	41.0
44-45	31.490801151405176	37.0	30.0	40.0	2.0	41.0
46-47	31.262956798303996	36.5	30.0	40.0	2.0	41.0
48-49	30.979954621724016	36.0	29.0	39.0	2.0	40.5
50-51	29.824765718240776	34.5	27.5	38.0	2.0	39.5
52-53	30.274102861834074	35.5	28.5	38.5	2.0	39.5
54-55	30.95450661288936	36.0	29.0	39.0	2.0	40.5
56-57	30.928971535957167	36.0	29.0	39.0	2.0	41.0
58-59	30.704151544044336	36.0	29.0	39.0	2.0	41.0
60-61	30.400790005219186	35.0	28.5	39.0	2.0	40.5
62-63	30.030464404822816	35.0	28.0	39.0	2.0	40.0
64-65	29.63218210776312	35.0	28.0	38.5	2.0	40.0
66-67	29.264102643857683	34.0	27.5	38.0	2.0	40.0
68-69	28.822449412945495	34.0	26.0	37.5	2.0	40.0
70-71	28.501461299026623	34.0	26.0	37.0	2.0	39.0
72-73	28.047229483339624	34.0	26.0	36.0	2.0	39.0
74-75	27.61204329353999	33.5	26.0	36.0	2.0	39.0
76-77	27.193375917919397	33.0	25.0	35.5	2.0	38.0
78-79	26.729242585924943	33.0	24.5	35.0	2.0	37.0
80-81	26.283509522751714	33.0	23.5	35.0	2.0	36.5
82-83	25.809733062193562	32.0	21.5	35.0	2.0	36.0
84-85	25.414714900351726	32.0	20.0	35.0	2.0	36.0
86-87	24.959288010735214	32.0	18.0	34.5	2.0	35.0
88-89	24.430280986262346	31.5	11.5	34.0	2.0	35.0
90-91	23.944247027500047	31.0	4.5	34.0	2.0	35.0
92-93	23.421661734440587	31.0	2.0	34.0	2.0	35.0
94-95	22.802838885322622	31.0	2.0	34.0	2.0	35.0
96-97	22.165528331909954	30.0	2.0	34.0	2.0	35.0
98	21.780070394128256	30.0	2.0	34.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	185766.0
3	31247.0
4	22802.0
5	18365.0
6	17072.0
7	17440.0
8	20273.0
9	23038.0
10	25583.0
11	27570.0
12	28713.0
13	29293.0
14	27321.0
15	28448.0
16	30931.0
17	33944.0
18	35235.0
19	34710.0
20	35968.0
21	38323.0
22	42140.0
23	47253.0
24	54139.0
25	62575.0
26	73168.0
27	84662.0
28	98192.0
29	114913.0
30	133854.0
31	158457.0
32	189536.0
33	230866.0
34	288999.0
35	368342.0
36	476607.0
37	551477.0
38	357721.0
39	38065.0
40	3.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.83710610937219	17.601105980541096	12.187126675634032	25.374661234452677
2	16.260612722503687	19.77008391207177	38.61929818415482	25.350005181269726
3	20.072117622274156	22.960447991534018	28.80582134230884	28.161613043882983
4	13.532595795382088	15.218670947238103	36.21770556504498	35.031027692334824
5	14.511401050444872	36.396420452318424	34.37191333829812	14.720265158938581
6	33.84748913303362	34.70873786407767	17.092052710176343	14.351720292712372
7	29.3878529832075	31.216058718142044	20.698688865275432	18.697399433375026
8	26.71268090889028	34.196155486303134	20.05812892560688	19.033034679199705
9	26.651777328055203	15.923836599999364	19.363332599397847	38.06105347254759
10-11	25.553828480235357	25.23422362004702	27.735123385061094	21.476824514656524
12-13	26.354958847567794	22.528695627042023	27.439702351947194	23.676643173442987
14-15	23.245860893495436	24.241014811581806	25.00867033759943	27.504453957323328
16-17	23.46405550530386	27.268214974058125	25.749985120436293	23.517744400201725
18-19	23.419193017379985	26.544491233443075	27.10974044866396	22.926575300512976
20-21	24.114147125794442	25.954812728712152	26.883803809397754	23.04723633609565
22-23	23.728695347763114	25.777489264936175	26.772756423142436	23.721058964158274
24-25	23.45774318333791	25.821222767386086	27.02318104403108	23.69785300524492
26-27	23.377318239348867	26.120161684847883	26.8103149089861	23.692205166817157
28-29	23.410799827089846	26.444581511356297	26.65531576197604	23.489302899577822
30-31	23.6727679100353	26.10709013316827	26.521485083092795	23.698656873703637
32-33	23.452628293841553	26.1311582752573	26.479228645292434	23.936984785608715
34-35	23.74558585241354	26.06275913668188	26.596997095592567	23.594657915312016
36-37	23.80604421076422	25.904156093487302	26.538608405052873	23.751191290695612
38-39	23.63466162257909	25.916103941901287	26.628729372996172	23.82050506252345
40-41	23.7691001906712	25.763180092917604	26.76383781319391	23.70388190321729
42-43	23.66853163538775	25.71049404657215	26.57966622430543	24.041308093734663
44-45	23.558128577197124	25.806552360304565	26.727641426703148	23.90767763579516
46-47	23.56134617574217	25.793811907910218	26.63351795914985	24.011323957197764
48-49	23.35145275215952	25.833136516766004	26.634807996576193	24.180602734498283
50-51	23.27021841551444	25.95716765269895	26.75540260532834	24.017211326458277
52-53	23.39849015094677	25.79042328743312	26.671018901467892	24.140067660152216
54-55	23.132442260110995	25.64594976577067	26.984380241650907	24.237227732467428
56-57	23.103118050415116	25.792776115005033	26.873730758223964	24.230375076355887
58-59	23.083341725531945	25.546184574511845	26.923046182422667	24.447427517533544
60-61	22.96540846663982	25.496779430166672	27.10301211672144	24.43479998647207
62-63	22.97840565343844	25.41141837377329	27.143063007056963	24.467112965731307
64-65	22.814135555665448	25.32980822765766	27.053193224652418	24.802862992024473
66-67	22.8350021015455	25.42539070536135	26.967725773413036	24.77188141968011
68-69	22.969705257224202	25.244122827771665	26.97354344063162	24.812628474372513
70-71	22.77196713150471	25.077469592954294	27.07203658064397	25.078526694897025
72-73	22.74461202049324	25.0351316493575	27.203904166612386	25.016352163536876
74-75	22.76609290096579	25.08808252336815	27.143462232678928	25.002362342987134
76-77	22.784715714045756	24.945495152714944	27.21352101129147	25.056268121947827
78-79	22.765744298082698	24.997017211946538	27.249006832949984	24.988231657020783
80-81	22.81076631566632	24.88142339608506	27.256752973540156	25.051057314708462
82-83	22.79476635091364	24.821294422316488	27.324382990013724	25.059556236756148
84-85	22.809238205079208	24.762331954291934	27.300337520023543	25.12809232060531
86-87	22.875949102056133	24.680941728445184	27.346143912341393	25.096965257157294
88-89	22.893046135206554	24.451239060676063	27.367191518194755	25.288523285922633
90-91	22.91719027699104	24.462463945396472	27.431142451463554	25.18920332614894
92-93	23.100588385650664	24.207977369026434	27.46251929334736	25.22891495197554
94-95	23.040311389173052	24.0874351538831	27.545313013859236	25.32694044308461
96-97	23.221301138994043	23.91124304205143	27.461371426057312	25.40608439289721
98	23.340228260089482	23.74507581306228	27.42340781754923	25.49128810929901
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	132.0
1	107.0
2	144.5
3	244.5
4	257.0
5	284.5
6	454.0
7	797.5
8	1206.5
9	1371.5
10	1347.5
11	1447.5
12	1609.0
13	1799.5
14	2062.0
15	2257.0
16	2379.0
17	2530.5
18	2593.0
19	2602.0
20	2673.0
21	2798.0
22	3034.0
23	3402.0
24	4018.0
25	4827.0
26	5994.0
27	7568.0
28	9630.5
29	12903.0
30	17992.0
31	25641.5
32	34367.5
33	44032.5
34	55003.5
35	66705.0
36	79521.0
37	92424.5
38	104479.0
39	115928.5
40	127996.5
41	138480.5
42	148979.0
43	159896.0
44	168556.0
45	176122.5
46	183002.5
47	188179.0
48	191239.5
49	191705.0
50	190900.0
51	189035.0
52	184407.0
53	178440.5
54	169917.5
55	157714.5
56	141309.5
57	125066.0
58	111144.5
59	94452.5
60	77624.0
61	63311.0
62	50520.5
63	38412.5
64	27995.5
65	20908.0
66	15896.5
67	12236.5
68	9050.0
69	6489.0
70	4689.0
71	3401.5
72	2522.0
73	1845.0
74	1344.5
75	936.0
76	633.0
77	408.0
78	231.0
79	125.5
80	80.0
81	49.0
82	26.5
83	25.0
84	22.0
85	12.5
86	9.5
87	7.5
88	7.5
89	9.0
90	7.0
91	6.0
92	6.5
93	6.0
94	5.0
95	3.5
96	1.5
97	1.0
98	1.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3837853975901608
2	0.024491729265485693
3	0.019372957848999183
4	0.016899293193185127
5	0.02280179994616718
6	0.02240993227791941
7	0.014523595454433016
8	0.00886600599410582
9	0.0
10-11	1.2245864632742847E-5
12-13	0.004959575176260852
14-15	0.0030614661581857116
16-17	0.005633097731061709
18-19	0.03547626984105602
20-21	0.07594885245227113
22-23	0.09441561631844735
24-25	0.06334785774517873
26-27	0.07286289456481994
28-29	0.0558166509960419
30-31	0.05195920363672789
32-33	0.019360711984366438
34-35	0.33922269619160955
36-37	0.5202043295989162
38-39	0.47453950038341797
40-41	0.4833075394604619
42-43	0.43365055837468963
44-45	0.40727296595576157
46-47	0.40626880505587665
48-49	0.39665580131917355
50-51	0.3927983539598595
52-53	0.451443799686065
54-55	0.45337864629803837
56-57	0.42482128997448204
58-59	0.40985684339327033
60-61	0.42489476516227853
62-63	0.40484828475847845
64-65	0.451921388406742
66-67	0.4735475853481659
68-69	0.41697169074489393
70-71	0.3743805735522143
72-73	0.36105707283179006
74-75	0.34184331122301653
76-77	0.3398839728817777
78-79	0.33875735333556534
80-81	0.3562077104372239
82-83	0.35212983751452054
84-85	0.35178695330480375
86-87	0.337251111985738
88-89	0.38145868330993965
90-91	0.3600896495258034
92-93	0.34903163376243657
94-95	0.3559383014153036
96-97	0.3564158901359805
98	0.3506970713524896
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
98	4083011.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.043374730752504
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.53743752740954	39.742112930330954
2	12.671861909650984	13.696603634392341
3	5.309595792846611	8.608454253049091
4	2.718490481952916	5.876655992726616
5	1.6066260071095735	4.341374567719766
6	1.039153115360364	3.3695604729629416
7	0.6881482681870523	2.603289830956587
8	0.49088895231654134	2.122343648098347
9	0.35059651213120285	1.705267681596127
>10	1.5145995016743115	13.665613577862235
>50	0.052557577957012505	1.9139123954215878
>100	0.018951009884279918	1.754452602867408
>500	6.377837521552468E-4	0.21894557003362256
>1k	4.5555976733005336E-4	0.3814128419823604
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	7.347518779645708E-5
2	0.0	0.0	0.0	2.4491729265485693E-5	7.347518779645708E-5
3	0.0	0.0	0.0	2.4491729265485693E-5	7.347518779645708E-5
4	0.0	0.0	0.0	2.4491729265485693E-5	7.347518779645708E-5
5	0.0	0.0	0.0	2.4491729265485693E-5	9.796691706194277E-5
6	0.0	0.0	0.0	2.4491729265485693E-5	9.796691706194277E-5
7	0.0	0.0	0.0	2.4491729265485693E-5	9.796691706194277E-5
8	0.0	0.0	0.0	2.4491729265485693E-5	9.796691706194277E-5
9	0.0	0.0	0.0	2.4491729265485693E-5	9.796691706194277E-5
10-11	0.0	0.0	0.0	4.8983458530971386E-5	1.7144210485839985E-4
12-13	0.0	0.0	0.0	4.8983458530971386E-5	3.306383450840569E-4
14-15	0.0	1.2245864632742847E-5	0.0	6.122932316371423E-5	4.2860526214599966E-4
16-17	0.0	2.4491729265485693E-5	0.0	9.796691706194277E-5	4.898345853097139E-4
18-19	0.0	4.8983458530971386E-5	0.0	1.1021278169468562E-4	5.265721792079424E-4
20-21	0.0	4.8983458530971386E-5	0.0	1.9593383412388555E-4	5.63309773106171E-4
22-23	0.0	4.8983458530971386E-5	0.0	5.143263145751995E-4	6.122932316371423E-4
24-25	0.0	7.347518779645708E-5	0.0	0.0013103075157034844	6.612766901681137E-4
26-27	0.0	9.796691706194277E-5	0.0	0.0026573526253051974	7.347518779645708E-4
28-29	0.0	9.796691706194277E-5	0.0	0.01131517892065439	7.347518779645708E-4
30-31	0.0	9.796691706194277E-5	0.0	0.0515305983745819	7.714894718627993E-4
32-33	0.0	9.796691706194277E-5	0.0	0.13373708765418463	8.082270657610278E-4
34-35	0.0	9.796691706194277E-5	0.0	0.24675417234976832	8.572105242919992E-4
36-37	0.0	9.796691706194277E-5	0.0	0.42357221178194226	8.572105242919992E-4
38-39	0.0	9.796691706194277E-5	0.0	0.7015166013513067	9.306857120884563E-4
40-41	0.0	9.796691706194277E-5	0.0	1.0155373081287316	9.306857120884563E-4
42-43	0.0	1.1021278169468562E-4	0.0	1.3447551329153902	9.306857120884563E-4
44-45	0.0	1.347045109601713E-4	0.0	1.7445091379866475	9.674233059866848E-4
46-47	0.0	1.4695037559291416E-4	0.0	2.2119215451538095	0.0010041608998849134
48-49	0.0	1.4695037559291416E-4	0.0	2.700249889113696	0.001028652629150399
50-51	0.0	1.4695037559291416E-4	0.0	3.232124527707616	0.0010531443584158846
52-53	0.0	1.4695037559291416E-4	0.0	3.7606315535275314	0.0011021278169468561
54-55	0.0	1.4695037559291416E-4	0.0	4.361315705492833	0.0011143736815795988
56-57	0.0	1.4695037559291416E-4	0.0	5.054933724156021	0.0011633571401105703
58-59	2.4491729265485693E-5	1.4695037559291416E-4	0.0	5.781982953266597	0.0011756030047433133
60-61	2.4491729265485693E-5	1.4695037559291416E-4	0.0	6.532654455253733	0.001187848869376056
62-63	4.8983458530971386E-5	1.4695037559291416E-4	0.0	7.295277921122427	0.0012245864632742846
64-65	4.8983458530971386E-5	1.4695037559291416E-4	0.0	8.109934065815644	0.0012245864632742846
66-67	4.8983458530971386E-5	1.59196240225657E-4	0.0	9.04534668165234	0.0012368323279070273
68-69	4.8983458530971386E-5	1.7144210485839985E-4	0.0	10.034126775558526	0.0012490781925397702
70-71	4.8983458530971386E-5	1.7144210485839985E-4	0.0	11.06171401448588	0.0012613240571725131
72-73	4.8983458530971386E-5	1.836879694911427E-4	0.0	12.099342862412078	0.0012980616510707417
74-75	4.8983458530971386E-5	1.9593383412388555E-4	0.0	13.16644750650929	0.0013715368388671988
76-77	4.8983458530971386E-5	1.9593383412388555E-4	0.0	14.327086064671391	0.00142052029739817
78-79	4.8983458530971386E-5	1.9593383412388555E-4	0.0	15.559215980559445	0.0014450120266636557
80-81	4.8983458530971386E-5	1.9593383412388555E-4	0.0	16.804681643032556	0.0014572578912963987
82-83	4.8983458530971386E-5	1.9593383412388555E-4	0.0	18.06833241448529	0.0015429789437255985
84-85	4.8983458530971386E-5	1.9593383412388555E-4	0.0	19.34937231371652	0.0015552248083583414
86	4.8983458530971386E-5	1.9593383412388555E-4	0.0	20.333205078311078	0.0015674706729910844
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	5860	0.0	42.645405	1
GGTATCA	3400	0.0	35.19366	1
TCAACGC	7015	0.0	34.842804	4
CAACGCA	7155	0.0	34.032864	5
ATCAACG	7150	0.0	33.992523	3
TATCAAC	7260	0.0	33.478718	2
AACGCAG	7585	0.0	32.402233	6
ACGCAGA	9230	0.0	25.979042	7
GTACATG	10045	0.0	25.107338	1
CGCAGAG	9635	0.0	24.600618	8
TACATGG	10365	0.0	23.980572	2
ACATGGG	10720	0.0	22.244474	3
TAAGGCG	920	0.0	21.433556	5
GTATAGG	785	0.0	21.105852	1
GCAGAGT	11050	0.0	20.495628	9
CATGGGG	7090	0.0	19.27452	4
GAGTACA	7255	0.0	18.713484	1
AGAGTAC	8835	0.0	18.188044	10-11
AGTACAT	7410	0.0	17.14317	2
CGTATCA	595	0.0	17.01316	92
>>END_MODULE