FastQCFastQC Report
Mon 15 Aug 2016
SRR2920879_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920879_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4083011
Sequences flagged as poor quality0
Sequence length101
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA32500.041.8364451
GTATCAA57150.041.2607881
TCAACGC70300.032.745344
ATCAACG71050.032.5332873
CAACGCA71650.032.0581865
TATCAAC73300.031.5327192
AACGCAG76500.030.150556
GTACATG104000.024.639251
ACGCAGA93700.024.311787
CGCAGAG96800.023.2872058
TACATGG106400.023.2398072
ACATGGG109700.021.5902163
GTGTAAG13300.019.6599851
GAGTACA76150.019.4785561
GCAGAGT110350.019.2235839
GTATAGG7800.018.2851581
CATGGGG76200.017.9390984
AGAGTAC90000.017.9005810-11
AGTACAT77800.017.6300962
CAGAGTA98650.016.1385810-11