##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920879_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4083011
Sequences flagged as poor quality	0
Sequence length	101
%GC	48
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.174474425858758	31.0	30.0	34.0	26.0	34.0
2	30.18723878040005	31.0	30.0	34.0	26.0	34.0
3	30.33705591290349	31.0	30.0	34.0	26.0	34.0
4	33.9660057246968	35.0	35.0	37.0	30.0	37.0
5	33.47659533613796	35.0	35.0	37.0	28.0	37.0
6	33.41625261357366	35.0	35.0	37.0	28.0	37.0
7	33.22339347114176	35.0	33.0	37.0	27.0	37.0
8	33.15842377108462	35.0	33.0	37.0	27.0	37.0
9	34.47391030785859	38.0	34.0	39.0	27.0	39.0
10-11	34.29127033946271	37.0	34.0	39.0	27.0	39.0
12-13	34.10452445511413	37.0	34.0	39.0	26.0	39.0
14-15	35.01244914598565	38.0	34.0	40.0	25.0	41.0
16-17	34.894450688474755	38.0	34.0	40.0	25.0	41.0
18-19	34.81203993817308	38.0	33.5	40.0	25.0	41.0
20-21	34.69606008898825	38.0	34.0	40.0	24.5	41.0
22-23	34.555512463718564	38.0	33.5	40.0	24.0	41.0
24-25	34.385504594525955	38.0	33.0	40.0	23.5	41.0
26-27	34.217679795621414	38.0	33.0	40.0	23.0	41.0
28-29	34.163351262095546	38.0	33.0	40.0	22.5	41.0
30-31	34.15009756280353	38.0	33.0	40.0	22.0	41.0
32-33	34.008914254700755	38.0	33.0	40.0	21.5	41.0
34-35	33.85025928169187	38.0	33.0	40.0	20.0	41.0
36-37	33.67019523092149	38.0	33.0	40.0	18.5	41.0
38-39	33.49347258677481	38.0	32.0	40.0	18.0	41.0
40-41	33.279606520775964	38.0	32.0	40.0	16.0	41.0
42-43	33.315631650269864	38.0	32.0	40.0	16.0	41.0
44-45	33.18368086688966	38.0	32.0	40.0	15.0	41.0
46-47	32.952813009810654	38.0	32.0	40.0	14.0	41.0
48-49	32.71234488469416	37.0	31.0	40.0	10.5	41.0
50-51	32.47164323093912	37.0	31.0	40.0	9.0	41.0
52-53	32.22719789390722	37.0	31.0	40.0	8.0	41.0
54-55	31.96605434077939	37.0	30.5	40.0	7.0	41.0
56-57	31.65607623883453	36.0	30.0	39.5	4.0	41.0
58-59	31.34216794909443	36.0	30.0	39.0	2.0	41.0
60-61	31.033936964656718	35.5	29.0	39.0	2.0	40.0
62-63	30.72468957345449	35.0	29.0	39.0	2.0	40.0
64-65	30.33475724165328	35.0	28.5	38.5	2.0	40.0
66-67	29.913061708626305	34.5	28.0	38.0	2.0	40.0
68-69	29.47768044220307	34.0	28.0	37.5	2.0	40.0
70-71	29.05701245967743	34.0	27.5	37.0	2.0	39.0
72-73	28.623404271994367	34.0	26.0	36.0	2.0	39.0
74-75	28.18438561639927	34.0	26.0	36.0	2.0	39.0
76-77	26.694221862248227	31.5	25.0	34.5	2.0	36.5
78-79	27.541442455090127	33.0	26.0	35.0	2.0	37.0
80-81	27.562280018349206	33.0	26.0	35.0	2.0	37.0
82-83	27.233696161974585	33.0	26.0	35.0	2.0	36.0
84-85	26.9223865671682	33.0	25.5	35.0	2.0	36.0
86-87	26.394338148978782	33.0	24.5	35.0	2.0	35.0
88-89	26.03688454427382	32.5	23.5	35.0	2.0	35.0
90-91	25.688441691682925	32.0	22.0	35.0	2.0	35.0
92-93	25.394383213760626	32.0	20.0	35.0	2.0	35.0
94-95	25.038112437120546	32.0	19.0	34.0	2.0	35.0
96-97	24.539724237823506	32.0	12.5	34.0	2.0	35.0
98-99	23.975775353042152	32.0	2.0	34.0	2.0	35.0
100-101	21.81063815404857	28.0	2.0	33.0	2.0	34.5
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	103045.0
3	45577.0
4	23784.0
5	16516.0
6	14025.0
7	13833.0
8	15494.0
9	17964.0
10	20216.0
11	22875.0
12	25331.0
13	27348.0
14	29558.0
15	31524.0
16	32969.0
17	34602.0
18	33863.0
19	32338.0
20	32659.0
21	34255.0
22	37115.0
23	41919.0
24	49274.0
25	57725.0
26	68587.0
27	81520.0
28	96871.0
29	113619.0
30	134043.0
31	158783.0
32	190805.0
33	232325.0
34	292630.0
35	379781.0
36	509308.0
37	621979.0
38	381576.0
39	27374.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.93384654683887	18.847590949925124	12.786569222845198	25.431993280390806
2	16.231942554159172	20.763622728422725	37.58552205712892	25.418912660289184
3	19.776127811629518	23.776609868247654	29.104682709746065	27.34257961037676
4	13.878276376044257	16.106132476983767	35.83159111757783	34.18400002939415
5	14.444837154665041	36.746581155444915	34.37768125760672	14.430900432283329
6	33.806791853306564	35.229692721473214	16.832969247629766	14.130546177590464
7	29.32523240065672	31.437820714377864	20.6530126120063	18.583934272959116
8	26.69836370127985	34.5304934987484	20.000152035907938	18.77099076406381
9	26.47562552777776	15.887450251236315	19.321319805544785	38.31560441544114
10-11	25.593889291216776	25.386734360734497	27.755658938906276	21.26371740914245
12-13	26.350423497487164	22.57934838750728	27.504183973972868	23.566044141032688
14-15	23.155078804329502	24.333928557198192	24.97744463222705	27.53354800624525
16-17	23.477488232238564	27.46369373099644	25.6957938240896	23.3630242126754
18-19	23.44814012121236	26.681241791840836	27.054497029498144	22.816121057448658
20-21	24.11751641213851	26.00705156294618	26.823478046647725	23.051953978267584
22-23	23.714507829109834	25.902913130640158	26.736496793998548	23.646082246251453
24-25	23.53890832847922	25.916912476263455	26.93962089653991	23.60455829871741
26-27	23.43744409202508	26.19693919614074	26.77122591453806	23.594390797296125
28-29	23.4879899563789	26.454009062829353	26.65582723952451	23.402173741267234
30-31	23.712544881101596	26.1094985577661	26.519637078119484	23.658319483012825
32-33	23.47147483518286	26.138952924385684	26.466853867137647	23.92271837329381
34-35	23.69145023621451	26.162115261119585	26.588208981895917	23.558225520769987
36-37	23.79231420114835	25.97864489706327	26.539935355566435	23.689105546221946
38-39	23.648331397263924	25.960393936531958	26.621021732608785	23.77025293359533
40-41	23.789348641892865	25.82241514027545	26.757658311706813	23.630577906124874
42-43	23.64211707750576	25.779603856304217	26.58141247258392	23.996866593606107
44-45	23.512227722475146	25.818757213952654	26.776514120085494	23.89250094348671
46-47	23.53679053698972	25.79844332981108	26.665553557730544	23.999212575468665
48-49	23.296193122239377	25.821435998351067	26.6823256635356	24.200045215873956
50-51	23.20844373194904	25.936788364491125	26.749197248507024	24.105570655052812
52-53	23.405124313817318	25.75655223022231	26.65854628714302	24.179777168817353
54-55	23.139455645674893	25.655335667857443	26.83551060039858	24.369698086069082
56-57	23.00138495351913	25.83356530192391	26.72648006880558	24.438569675751385
58-59	22.932724642664876	25.689773497120406	26.738547616843285	24.63895424337144
60-61	22.897192369594986	25.67249864652666	26.830826855850802	24.599482128027557
62-63	22.78461944313196	25.664291454699146	26.780900718981098	24.7701883831878
64-65	22.645558954713906	25.54477295717795	26.644348974088288	25.16531911401985
66-67	22.669851364436447	25.654813357795575	26.505086989928927	25.170248287839055
68-69	22.813859435724932	25.487098705237443	26.45738618674801	25.24165567228961
70-71	22.58568164881903	25.319721538622087	26.45445557312257	25.640141239436314
72-73	22.584864713612145	25.269961440555562	26.4615627000775	25.6836111457548
74-75	22.458223108163494	25.26405015482183	26.366837045854325	25.91088969116035
76-77	22.326768037805415	25.06992943894032	26.623286782455207	25.980015740799058
78-79	22.318647507707315	25.134546363697087	26.518964808311342	26.02784132028426
80-81	22.232284494541876	25.030362369180935	26.444227304037177	26.293125832240012
82-83	22.06169155642473	25.00268215638515	26.484249006009993	26.45137728118013
84-85	21.988458267926962	24.819475959460522	26.426032449073045	26.766033323539475
86-87	21.879885456926697	24.821967242783014	26.481768716353155	26.81637858393713
88-89	21.86791146946343	24.60771227193638	26.46760960872821	27.05676664987198
90-91	21.74332376275845	24.566119477314988	26.40000353616862	27.290553223757946
92-93	21.70797596735973	24.379481834136147	26.491007667897236	27.421534530606888
94-95	21.443166055610874	24.185930772536253	26.647637174055443	27.72326599779743
96-97	21.468060660582843	24.02947059761456	26.640649703185204	27.861819038617398
98-99	21.371548500734104	23.977575641125025	26.55516698194645	28.095708876194426
100-101	21.443862860913363	23.81023060417699	26.60292148671316	28.142985048196483
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	122.0
1	91.5
2	122.0
3	188.5
4	172.5
5	178.0
6	315.0
7	561.0
8	785.5
9	874.0
10	780.0
11	658.5
12	665.5
13	702.5
14	752.0
15	824.0
16	913.0
17	1018.0
18	1134.5
19	1277.0
20	1474.0
21	1682.5
22	1947.5
23	2385.5
24	2976.5
25	3647.0
26	4617.5
27	5986.0
28	7686.5
29	10150.0
30	14090.5
31	19717.0
32	27633.5
33	36338.0
34	45411.5
35	55532.0
36	66791.5
37	78929.5
38	90174.0
39	100456.0
40	112546.5
41	124162.0
42	134585.0
43	145846.5
44	155861.0
45	165030.0
46	172797.0
47	179435.0
48	185478.5
49	188792.5
50	190052.0
51	189803.5
52	186283.5
53	181809.0
54	174763.5
55	162264.0
56	147109.5
57	130458.5
58	114935.5
59	99473.5
60	82133.5
61	66302.0
62	52621.0
63	39720.5
64	29031.0
65	21593.0
66	16198.0
67	12329.5
68	9233.5
69	6559.0
70	4642.0
71	3435.5
72	2478.0
73	1816.0
74	1297.5
75	915.5
76	632.5
77	380.0
78	222.5
79	127.0
80	69.5
81	39.0
82	18.5
83	10.0
84	7.0
85	4.5
86	4.0
87	2.0
88	0.5
89	0.5
90	0.0
91	0.5
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.19039870330988576
2	0.0
3	0.012319339820539304
4	0.013862318764264902
5	0.006661750360212108
6	0.10533892757085396
7	0.14168465380083473
8	0.12311992301759656
9	0.14141524477891437
10-11	0.11419268770032703
12-13	0.1139232786784067
14-15	0.11544176589286681
16-17	0.06587050585952377
18-19	0.0790837937982533
20-21	0.03684780667992322
22-23	0.08318615845022215
24-25	0.055792159266776414
26-27	0.06478062390720965
28-29	0.03954189689912665
30-31	0.02389168189848129
32-33	0.031606576617109285
34-35	0.019850546569676154
36-37	0.024222320243565347
38-39	0.03153310142931283
40-41	0.02509177663249009
42-43	0.013543926283813588
44-45	0.02638983828356083
46-47	0.033063834508405686
48-49	0.009159906745291648
50-51	0.015809411240871013
52-53	0.006833192465070507
54-55	0.012809174405849015
56-57	0.004959575176260852
58-59	0.0036492676605573682
60-61	0.02255688265351232
62-63	0.010482460125627875
64-65	0.007372010508911194
66-67	0.039652109680821335
68-69	0.08946828700681923
70-71	0.021185345814645123
72-73	0.043595278092564534
74-75	0.08530469303168667
76-77	0.24872575655563994
78-79	0.08041859304322227
80-81	0.016042082668893128
82-83	0.011633571401105705
84-85	0.012772436811950788
86-87	0.2609961129176483
88-89	0.616689497040297
90-91	0.26468211817210385
92-93	0.3500970239854852
94-95	0.0012368323279070275
96-97	0.003967660141008683
98-99	0.0031716789398803973
100-101	0.004714657883605995
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4083011.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.96468673722451
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.58374667464727	37.198269714248816
2	13.357176080498293	13.882029414340838
3	5.774374393081215	9.001906693197483
4	3.0096513510853455	6.255823585896578
5	1.760930143796255	4.575309164425404
6	1.0965393429367132	3.4188794070448973
7	0.7759488534389732	2.8225357365145345
8	0.5223026393506245	2.17130344287046
9	0.3866092595391473	1.8081026155495905
>10	1.6503293688289493	14.211798191079106
>50	0.05980866858255836	2.0987396929783846
>100	0.021257663977759238	1.8605995537604532
>500	8.048044856221601E-4	0.2776941729279496
>1k	5.207557513421727E-4	0.417008615165439
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.4491729265485693E-5
2	0.0	0.0	0.0	0.0	2.4491729265485693E-5
3	0.0	0.0	0.0	0.0	2.4491729265485693E-5
4	0.0	0.0	0.0	0.0	2.4491729265485693E-5
5	0.0	0.0	0.0	0.0	2.4491729265485693E-5
6	0.0	0.0	0.0	0.0	2.4491729265485693E-5
7	0.0	0.0	0.0	0.0	2.4491729265485693E-5
8	0.0	0.0	0.0	0.0	2.4491729265485693E-5
9	0.0	0.0	0.0	0.0	2.4491729265485693E-5
10-11	0.0	0.0	0.0	0.0	3.673759389822854E-5
12-13	0.0	0.0	0.0	0.0	1.836879694911427E-4
14-15	0.0	0.0	0.0	1.2245864632742847E-5	2.4491729265485693E-4
16-17	0.0	0.0	0.0	2.4491729265485693E-5	2.694090219203426E-4
18-19	0.0	0.0	0.0	3.673759389822854E-5	2.694090219203426E-4
20-21	0.0	0.0	0.0	1.347045109601713E-4	3.428842097167997E-4
22-23	1.2245864632742847E-5	0.0	0.0	4.408511267787425E-4	3.428842097167997E-4
24-25	2.4491729265485693E-5	0.0	0.0	0.0012368323279070275	3.428842097167997E-4
26-27	2.4491729265485693E-5	0.0	0.0	0.002559385708243255	4.2860526214599966E-4
28-29	2.4491729265485693E-5	0.0	0.0	0.011106999221897762	4.408511267787425E-4
30-31	2.4491729265485693E-5	0.0	0.0	0.05074686303808636	4.6534285604422817E-4
32-33	2.4491729265485693E-5	0.0	0.0	0.1323288132214192	4.898345853097139E-4
34-35	2.4491729265485693E-5	0.0	0.0	0.2445989001744056	5.143263145751995E-4
36-37	2.4491729265485693E-5	0.0	0.0	0.42163736516996897	5.143263145751995E-4
38-39	2.4491729265485693E-5	0.0	0.0	0.7011614712769572	5.265721792079424E-4
40-41	2.4491729265485693E-5	0.0	0.0	1.0173007126358464	5.388180438406852E-4
42-43	2.4491729265485693E-5	1.2245864632742847E-5	0.0	1.3475594359162884	5.755556377389137E-4
44-45	2.4491729265485693E-5	3.673759389822854E-5	0.0	1.7486237485032492	6.000473670043995E-4
46-47	2.4491729265485693E-5	4.8983458530971386E-5	0.0	2.2160361556704107	6.122932316371423E-4
48-49	2.4491729265485693E-5	4.8983458530971386E-5	0.0	2.7004090853539213	6.857684194335994E-4
50-51	2.4491729265485693E-5	4.8983458530971386E-5	0.0	3.225217860054749	7.10260148699085E-4
52-53	2.4491729265485693E-5	4.8983458530971386E-5	0.0	3.7482509843838283	7.347518779645708E-4
54-55	2.4491729265485693E-5	4.8983458530971386E-5	0.0	4.341844780726772	7.592436072300564E-4
56-57	2.4491729265485693E-5	4.8983458530971386E-5	0.0	5.027711167077434	7.592436072300564E-4
58-59	2.4491729265485693E-5	4.8983458530971386E-5	0.0	5.748833397705761	7.592436072300564E-4
60-61	2.4491729265485693E-5	4.8983458530971386E-5	0.0	6.494998421508049	7.95981201128285E-4
62-63	2.4491729265485693E-5	4.8983458530971386E-5	0.0	7.248351767849757	8.327187950265136E-4
64-65	2.4491729265485693E-5	4.8983458530971386E-5	0.0	8.055917556920615	8.327187950265136E-4
66-67	2.4491729265485693E-5	4.8983458530971386E-5	0.0	8.982905017889983	8.449646596592564E-4
68-69	2.4491729265485693E-5	4.8983458530971386E-5	0.0	9.95908411708908	8.572105242919992E-4
70-71	2.4491729265485693E-5	4.8983458530971386E-5	0.0	10.974829604916568	8.69456388924742E-4
72-73	2.4491729265485693E-5	4.8983458530971386E-5	0.0	12.003298546097476	9.184398474557135E-4
74-75	2.4491729265485693E-5	4.8983458530971386E-5	0.0	13.064770092463625	0.0010041608998849134
76-77	2.4491729265485693E-5	4.8983458530971386E-5	0.0	14.22229820100901	0.0010776360876813705
78-79	2.4491729265485693E-5	4.8983458530971386E-5	0.0	15.447643907890525	0.0010776360876813705
80-81	2.4491729265485693E-5	7.347518779645708E-5	0.0	16.687684652331328	0.0010776360876813705
82-83	2.4491729265485693E-5	7.347518779645708E-5	0.0	17.94466142755922	0.0011021278169468561
84-85	2.4491729265485693E-5	7.347518779645708E-5	0.0	19.22132955311656	0.0011021278169468561
86-87	2.4491729265485693E-5	7.347518779645708E-5	0.0	20.544225817662507	0.0011266195462123418
88-89	2.4491729265485693E-5	7.347518779645708E-5	0.0	21.922461144483812	0.0011511112754778274
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	3250	0.0	41.836445	1
GTATCAA	5715	0.0	41.260788	1
TCAACGC	7030	0.0	32.74534	4
ATCAACG	7105	0.0	32.533287	3
CAACGCA	7165	0.0	32.058186	5
TATCAAC	7330	0.0	31.532719	2
AACGCAG	7650	0.0	30.15055	6
GTACATG	10400	0.0	24.63925	1
ACGCAGA	9370	0.0	24.31178	7
CGCAGAG	9680	0.0	23.287205	8
TACATGG	10640	0.0	23.239807	2
ACATGGG	10970	0.0	21.590216	3
GTGTAAG	1330	0.0	19.659985	1
GAGTACA	7615	0.0	19.478556	1
GCAGAGT	11035	0.0	19.223583	9
GTATAGG	780	0.0	18.285158	1
CATGGGG	7620	0.0	17.939098	4
AGAGTAC	9000	0.0	17.90058	10-11
AGTACAT	7780	0.0	17.630096	2
CAGAGTA	9865	0.0	16.13858	10-11
>>END_MODULE