FastQCFastQC Report
Mon 15 Aug 2016
SRR2920878_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920878_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4164337
Sequences flagged as poor quality0
Sequence length98
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA82150.040.5604671
GGTATCA45900.038.703171
ATCAACG101700.032.1505433
TCAACGC101700.031.9250834
CAACGCA103500.031.2812485
TATCAAC106200.031.1355532
AACGCAG107250.030.4396656
GTACATG142400.026.210951
ACGCAGA126200.025.4307587
TACATGG148200.024.4160082
CGCAGAG132650.023.812878
ACATGGG157000.022.8708253
GTATAGG9100.021.7469831
GCAGAGT145750.020.949869
CATGGGG106100.018.7583264
GAGTACA111200.018.58291
AGAGTAC122500.018.3392110-11
AGTACAT109550.017.5407222
CAGAGTA135550.016.70890810-11
GAGTACT54750.015.91397912-13
Produced by FastQC (version 0.11.5)