FastQCFastQC Report
Mon 15 Aug 2016
SRR2920878_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920878_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4164337
Sequences flagged as poor quality0
Sequence length101
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA82850.041.2692681
GGTATCA46800.036.8851171
ATCAACG99200.033.4517443
TCAACGC99950.033.200734
CAACGCA100850.032.6639675
TATCAAC106600.031.6613252
AACGCAG107900.030.7500846
GTACATG146200.025.8263781
ACGCAGA128400.025.1748837
TACATGG151700.024.9395962
CGCAGAG134550.023.951628
ACATGGG158600.023.2283
GCAGAGT151000.020.399369
CATGGGG106950.018.7320334
TAAGGCG7100.018.7191035
GAGTACA115200.018.6584821
AGAGTAC125500.017.88635610-11
CCGATCG4253.6379788E-1217.868169
AGTACAT113300.017.4293062
GAGTACT51750.016.45940612-13
Produced by FastQC (version 0.11.5)