##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920878_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4164337
Sequences flagged as poor quality	0
Sequence length	101
%GC	48
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.240925506269065	31.0	30.0	34.0	27.0	34.0
2	30.246990337237357	31.0	30.0	34.0	26.0	34.0
3	30.390431658148703	31.0	30.0	34.0	27.0	34.0
4	34.03250241274902	35.0	35.0	37.0	30.0	37.0
5	33.55108772416834	35.0	35.0	37.0	28.0	37.0
6	33.495439730261985	35.0	35.0	37.0	28.0	37.0
7	33.30565994058598	35.0	33.0	37.0	27.0	37.0
8	33.23780424110729	35.0	33.0	37.0	27.0	37.0
9	34.56847800742351	38.0	34.0	39.0	27.0	39.0
10-11	34.373612534240145	37.5	34.0	39.0	27.0	39.0
12-13	34.1934462796839	37.0	34.0	39.0	26.0	39.0
14-15	35.08682702672718	38.0	34.0	40.0	26.0	41.0
16-17	34.965334097600646	38.0	34.0	40.0	25.0	41.0
18-19	34.87359404390183	38.0	34.0	40.0	25.0	41.0
20-21	34.75815561997024	38.0	34.0	40.0	25.0	41.0
22-23	34.6032779047421	38.0	33.5	40.0	24.0	41.0
24-25	34.42304465272623	38.0	33.0	40.0	24.0	41.0
26-27	34.24104689413945	38.0	33.0	40.0	23.0	41.0
28-29	34.183230848031755	38.0	33.0	40.0	22.5	41.0
30-31	34.17019852139728	38.0	33.0	40.0	23.0	41.0
32-33	34.01795279776829	38.0	33.0	40.0	22.0	41.0
34-35	33.84922942115396	38.0	33.0	40.0	20.5	41.0
36-37	33.65404000684863	38.0	33.0	40.0	18.5	41.0
38-39	33.46703845053847	38.0	32.0	40.0	18.0	41.0
40-41	33.23419022043605	38.0	32.0	40.0	16.0	41.0
42-43	33.2568023433262	38.0	32.0	40.0	15.5	41.0
44-45	33.10764702760608	38.0	32.0	40.0	15.0	41.0
46-47	32.85978212137971	37.5	31.5	40.0	13.5	41.0
48-49	32.59343960395137	37.0	31.0	40.0	9.0	41.0
50-51	32.33388123967873	37.0	31.0	40.0	8.5	41.0
52-53	32.06147521202055	37.0	31.0	40.0	7.5	41.0
54-55	31.77184147200383	36.0	30.0	40.0	6.5	41.0
56-57	31.44149032607111	36.0	30.0	39.0	2.0	41.0
58-59	31.102008555023282	35.5	29.0	39.0	2.0	40.0
60-61	30.757439419528247	35.0	29.0	39.0	2.0	40.0
62-63	30.42785094001758	35.0	28.5	38.5	2.0	40.0
64-65	30.01665727341471	35.0	28.0	38.0	2.0	40.0
66-67	29.57681642960212	34.0	28.0	37.5	2.0	40.0
68-69	29.126833154953594	34.0	27.5	37.0	2.0	39.5
70-71	28.699850060165637	34.0	26.0	36.5	2.0	39.0
72-73	28.26152782543776	34.0	26.0	36.0	2.0	39.0
74-75	27.82267825586642	33.0	26.0	35.5	2.0	38.0
76-77	26.39460291037925	31.5	24.0	34.0	2.0	36.0
78-79	27.239675967627022	33.0	26.0	35.0	2.0	37.0
80-81	27.269880295470806	33.0	26.0	35.0	2.0	36.5
82-83	26.94512848023587	33.0	25.0	35.0	2.0	36.0
84-85	26.636263227495757	33.0	25.0	35.0	2.0	36.0
86-87	26.10693178770114	32.0	24.0	35.0	2.0	35.0
88-89	25.75125956424756	32.0	22.0	35.0	2.0	35.0
90-91	25.391256039076566	32.0	20.0	34.5	2.0	35.0
92-93	25.085710642534455	32.0	19.5	34.0	2.0	35.0
94-95	24.709807347484123	32.0	17.5	34.0	2.0	35.0
96-97	24.17888886033959	31.5	4.5	34.0	2.0	35.0
98-99	23.573925693333656	31.0	2.0	34.0	2.0	35.0
100-101	21.383198694053817	28.0	2.0	33.0	2.0	34.5
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	99968.0
3	45079.0
4	24243.0
5	17178.0
6	15004.0
7	14862.0
8	16647.0
9	19274.0
10	22033.0
11	24502.0
12	27074.0
13	29702.0
14	31795.0
15	33714.0
16	35890.0
17	37050.0
18	35718.0
19	34092.0
20	34556.0
21	35698.0
22	38968.0
23	43963.0
24	51107.0
25	60944.0
26	72257.0
27	85120.0
28	100794.0
29	118477.0
30	140006.0
31	165749.0
32	199097.0
33	242580.0
34	305641.0
35	396860.0
36	528247.0
37	615291.0
38	341722.0
39	23435.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.787136124549775	18.932578282075543	13.554401871364613	24.725883722010067
2	16.118844368263183	20.321698267935567	38.18391739189215	25.375539971909095
3	19.54049820669223	24.201225126770794	28.703683690001526	27.554592976535453
4	13.916613389833197	16.138581569754066	35.38397506500094	34.5608299754118
5	14.706887454974712	36.87281297602406	33.80493361149208	14.615365957509146
6	34.24259076828696	34.84116601496486	16.599044491208637	14.317198725539548
7	29.812826797994678	31.136407524058274	20.477974269065477	18.57279140888157
8	27.05081332382488	34.56168224613655	19.495054946904457	18.892449483134115
9	26.981120595929966	15.640275773439456	19.02898295257652	38.34962067805406
10-11	26.052503589985974	25.30093254288329	27.101094437847745	21.54546942928299
12-13	26.628995843531772	22.35553153581128	27.291701175682604	23.723771444974336
14-15	23.66520199984541	24.04303098399621	24.64978603394727	27.641980982211106
16-17	23.91249393253459	27.160366114467237	25.21369493024217	23.713445022755998
18-19	23.885957372562956	26.364276615351745	26.685172610322194	23.06459340176311
20-21	24.767613508744745	25.541590545752424	26.319255017974747	23.371540927528084
22-23	24.306437297259492	25.375570893955707	26.29593498748678	24.022056821298023
24-25	24.00753595581264	25.587133708192912	26.398695407930102	24.006634928064347
26-27	23.966493038826812	25.76729816514533	26.377366497316046	23.88884229871181
28-29	23.972441242332597	25.849517959568974	26.33600865982755	23.842032138270884
30-31	24.167174175454846	25.70315137436903	26.110315336797484	24.019359113378645
32-33	23.99856257627456	25.737163084297194	26.098608852959167	24.165665486469084
34-35	24.272925248691067	25.684934714767554	26.156257032846653	23.88588300369473
36-37	24.282117569123304	25.607729982867045	26.12696690934953	23.983185538660123
38-39	24.238996138486037	25.605965595281006	26.095996293061894	24.059041973171063
40-41	24.23557797104095	25.551399523333995	26.198567600042516	24.014454905582543
42-43	24.24124779949133	25.378031178175757	26.190952517045684	24.189768505287223
44-45	24.069102190116773	25.48899885437385	26.16963697978887	24.272261975720507
46-47	24.057527868349187	25.441940233559535	26.118148036180838	24.38238386191044
48-49	24.04588347262705	25.518848179735603	26.014323305354516	24.42094504228283
50-51	23.9491129653502	25.696055162045113	26.02383798671024	24.330993885894447
52-53	24.026738375656887	25.53293936615833	26.09705329123492	24.343268966949864
54-55	23.827929491804177	25.472305772476673	26.18705671800084	24.51270801771831
56-57	23.80514055852624	25.5140732632673	26.074707990488744	24.606078187717713
58-59	23.735771204840137	25.398367841536114	26.149992591576876	24.715868362046876
60-61	23.753844387527472	25.388323822418567	26.215537420426628	24.64229436962733
62-63	23.54514848562827	25.506352605350507	26.17570960451584	24.77278930450538
64-65	23.57224336883563	25.350067842605156	26.1311703749955	24.946518413563716
66-67	23.566599502121537	25.407140261791582	25.98054730596103	25.045712930125852
68-69	23.593941568768926	25.22444754286033	26.05646514438359	25.125145743987154
70-71	23.398122356884777	25.116965392078754	26.074521588188148	25.410390662848325
72-73	23.30807622466386	25.089307196696375	26.080932698788466	25.52168387985129
74-75	23.159363854921313	25.170143644810107	26.080772956984426	25.589719543284158
76-77	23.22597876162264	24.967394271502087	26.219591457082487	25.587035509792795
78-79	23.17141225633971	24.98559211481318	26.133993091905726	25.709002536941384
80-81	23.032242398973207	24.99487228507309	26.152883240850333	25.82000207510337
82-83	22.919284693678684	24.918797883251237	26.179305465377677	25.982611957692402
84-85	22.876086827301858	24.80866856037749	26.148075697998152	26.16716891432249
86-87	22.730937221883217	24.77527652324483	26.225874676341736	26.267911578530217
88-89	22.686401519004836	24.707907383812344	26.11779053226786	26.487900564914963
90-91	22.51743065809425	24.58455050481642	26.299596038516892	26.59842279857244
92-93	22.492031750576082	24.53240003720846	26.233903846978762	26.741664365236694
94-95	22.328021414490802	24.414803220626453	26.297540296853022	26.95963506802972
96-97	22.34340051458182	24.231057034819525	26.34825575782667	27.077286692771985
98-99	22.284117412002473	24.23745403687808	26.256385367877876	27.222043183241567
100-101	22.386399289840593	24.009197107058597	26.308257740412145	27.296145862688665
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	147.0
1	107.5
2	107.0
3	159.5
4	132.5
5	132.5
6	245.5
7	499.0
8	768.5
9	815.5
10	685.0
11	567.0
12	592.5
13	673.5
14	727.0
15	809.0
16	927.0
17	1033.5
18	1101.5
19	1250.5
20	1472.0
21	1687.5
22	2006.0
23	2435.5
24	2999.0
25	3796.0
26	4959.5
27	6483.0
28	8570.0
29	11633.5
30	16192.0
31	22326.0
32	30095.5
33	38111.5
34	45382.0
35	53245.5
36	63635.5
37	73894.5
38	83948.0
39	93239.5
40	101715.5
41	111994.5
42	122192.0
43	134045.5
44	145227.5
45	153685.5
46	163370.5
47	173033.0
48	181046.5
49	188425.0
50	193386.5
51	193951.0
52	191201.5
53	188061.0
54	182978.5
55	174051.5
56	161084.5
57	145057.0
58	130573.0
59	116092.5
60	98182.0
61	80586.5
62	64955.0
63	49438.5
64	36324.5
65	27189.0
66	20152.0
67	14851.0
68	11159.0
69	8394.0
70	6300.5
71	4789.5
72	3734.0
73	3133.5
74	2411.0
75	1669.5
76	1136.5
77	611.5
78	300.5
79	145.0
80	81.0
81	43.5
82	24.0
83	11.5
84	8.5
85	7.0
86	3.0
87	0.5
88	1.0
89	1.0
90	0.0
91	0.5
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1872566989655256
2	0.0
3	0.012559022000380855
4	0.013231397939215774
5	0.006915866799444905
6	0.10707586825946123
7	0.14398450461622103
8	0.12561423343019548
9	0.14398450461622103
10-11	0.11646511797676316
12-13	0.11516839295186725
14-15	0.11803799740510913
16-17	0.06716555360433125
18-19	0.08000073000816216
20-21	0.03726883775256421
22-23	0.08495950255706972
24-25	0.05818453213560766
26-27	0.0661329762696919
28-29	0.040042388500258264
30-31	0.024649782186215958
32-33	0.03202190408701313
34-35	0.020123251312273716
36-37	0.024397641209152863
38-39	0.031997890660626166
40-41	0.02508202386118126
42-43	0.0126550757059287
44-45	0.026690923429107685
46-47	0.03399100505074397
48-49	0.008500752940984364
50-51	0.014516116250918212
52-53	0.00683181980709054
54-55	0.012222834030963393
56-57	0.0052229202391641215
58-59	0.0037941213691399136
60-61	0.023112922897450424
62-63	0.009977578663782495
64-65	0.006891853373057945
66-67	0.040210482484966996
68-69	0.09096285915381008
70-71	0.02223643283432633
72-73	0.045109221467907135
74-75	0.08616017387641778
76-77	0.24395239866514168
78-79	0.08214993166979521
80-81	0.016545250780616458
82-83	0.011970693053900297
84-85	0.01331544493157014
86-87	0.26038958902701675
88-89	0.6204589109863107
90-91	0.2664169590501441
92-93	0.3540299452229731
94-95	0.0012967250248959198
96-97	0.004298403323266104
98-99	0.003097732003918031
100-101	0.004838705416972737
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4164337.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.9917531378922
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.53108245849702	27.682174612733522
2	12.233075832490988	10.029104492851712
3	6.0568502966508	7.448427364604362
4	3.644891848293854	5.976420274383091
5	2.373416151024056	4.864524447813219
6	1.6901533348504152	4.156940896042408
7	1.1542584548990467	3.312055434838262
8	0.8964763741193242	2.9398510577481627
9	0.7029304102256386	2.5932914864178005
>10	3.557943076628061	24.564132437390242
>50	0.11770470965261604	3.262522508218969
>100	0.04021599213953974	2.7592855009143005
>500	7.066310096934234E-4	0.19083794318375957
>1k	2.944295188687309E-4	0.22043154286020641
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	2.4013426386961477E-5	0.0
10-11	0.0	0.0	0.0	2.4013426386961477E-5	0.0
12-13	0.0	0.0	0.0	3.602013958044221E-5	0.0
14-15	0.0	0.0	0.0	4.802685277392295E-5	1.2006713193480738E-5
16-17	0.0	2.4013426386961477E-5	0.0	4.802685277392295E-5	2.4013426386961477E-5
18-19	0.0	2.4013426386961477E-5	0.0	7.204027916088444E-5	2.4013426386961477E-5
20-21	0.0	2.4013426386961477E-5	0.0	1.0806041874132665E-4	7.204027916088444E-5
22-23	0.0	2.4013426386961477E-5	0.0	2.8816111664353776E-4	7.204027916088444E-5
24-25	0.0	2.4013426386961477E-5	0.0	0.0010325773346393434	7.204027916088444E-5
26-27	0.0	2.4013426386961477E-5	0.0	0.0019931143901178025	7.204027916088444E-5
28-29	0.0	2.4013426386961477E-5	0.0	0.0070839607841536355	7.204027916088444E-5
30-31	0.0	2.4013426386961477E-5	0.0	0.027555406779038297	7.204027916088444E-5
32-33	0.0	3.602013958044221E-5	0.0	0.0714399435012104	7.204027916088444E-5
34-35	0.0	4.802685277392295E-5	0.0	0.13021280458329862	7.204027916088444E-5
36-37	0.0	4.802685277392295E-5	0.0	0.229400262274643	7.204027916088444E-5
38-39	0.0	4.802685277392295E-5	0.0	0.3886332926465846	7.204027916088444E-5
40-41	0.0	4.802685277392295E-5	0.0	0.5754697566503384	7.204027916088444E-5
42-43	0.0	4.802685277392295E-5	0.0	0.7673250267689671	7.204027916088444E-5
44-45	0.0	4.802685277392295E-5	0.0	0.9927750804029549	7.204027916088444E-5
46-47	0.0	4.802685277392295E-5	0.0	1.2628060601243367	9.60537055478459E-5
48-49	0.0	4.802685277392295E-5	0.0	1.5576909361562237	9.60537055478459E-5
50-51	0.0	4.802685277392295E-5	0.0	1.870970096800523	9.60537055478459E-5
52-53	0.0	4.802685277392295E-5	0.0	2.193782587720446	9.60537055478459E-5
54-55	0.0	4.802685277392295E-5	0.0	2.5550645877122817	9.60537055478459E-5
56-57	0.0	4.802685277392295E-5	0.0	2.969308199600561	9.60537055478459E-5
58-59	0.0	4.802685277392295E-5	0.0	3.42294583747665	9.60537055478459E-5
60-61	0.0	4.802685277392295E-5	0.0	3.907572802105113	9.60537055478459E-5
62-63	0.0	4.802685277392295E-5	0.0	4.396330076072134	9.60537055478459E-5
64-65	0.0	4.802685277392295E-5	0.0	4.91787768377055	9.60537055478459E-5
66-67	0.0	4.802685277392295E-5	0.0	5.507647435834324	9.60537055478459E-5
68-69	0.0	4.802685277392295E-5	0.0	6.157870988827273	9.60537055478459E-5
70-71	0.0	4.802685277392295E-5	0.0	6.851210648898012	1.2006713193480739E-4
72-73	0.0	4.802685277392295E-5	0.0	7.558129901590577	1.2006713193480739E-4
74-75	0.0	4.802685277392295E-5	0.0	8.28463210350171	1.2006713193480739E-4
76-77	0.0	4.802685277392295E-5	0.0	9.069282817408869	1.2006713193480739E-4
78-79	0.0	4.802685277392295E-5	0.0	9.933646100207548	1.2006713193480739E-4
80-81	0.0	4.802685277392295E-5	0.0	10.8393124763918	1.3207384512828812E-4
82-83	0.0	6.003356596740369E-5	0.0	11.754212015021839	1.4408055832176888E-4
84-85	0.0	7.204027916088444E-5	0.0	12.669039513372716	1.4408055832176888E-4
86-87	0.0	7.204027916088444E-5	0.0	13.615684801686319	1.4408055832176888E-4
88-89	0.0	7.204027916088444E-5	0.0	14.633998161051807	1.4408055832176888E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	8285	0.0	41.269268	1
GGTATCA	4680	0.0	36.885117	1
ATCAACG	9920	0.0	33.451744	3
TCAACGC	9995	0.0	33.20073	4
CAACGCA	10085	0.0	32.663967	5
TATCAAC	10660	0.0	31.661325	2
AACGCAG	10790	0.0	30.750084	6
GTACATG	14620	0.0	25.826378	1
ACGCAGA	12840	0.0	25.174883	7
TACATGG	15170	0.0	24.939596	2
CGCAGAG	13455	0.0	23.95162	8
ACATGGG	15860	0.0	23.228	3
GCAGAGT	15100	0.0	20.39936	9
CATGGGG	10695	0.0	18.732033	4
TAAGGCG	710	0.0	18.719103	5
GAGTACA	11520	0.0	18.658482	1
AGAGTAC	12550	0.0	17.886356	10-11
CCGATCG	425	3.6379788E-12	17.86816	9
AGTACAT	11330	0.0	17.429306	2
GAGTACT	5175	0.0	16.459406	12-13
>>END_MODULE