FastQCFastQC Report
Mon 15 Aug 2016
SRR2920877_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920877_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3914341
Sequences flagged as poor quality0
Sequence length98
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA51700.040.0614661
GGTATCA29150.038.2104071
TCAACGC64300.031.6699774
ATCAACG65050.031.446253
CAACGCA65300.031.1849885
TATCAAC67850.030.0817152
AACGCAG70200.029.4595726
GTACATG106100.026.5919341
TACATGG111300.024.6844862
ACGCAGA82750.024.655427
ACATGGG115700.023.269453
CGCAGAG87800.022.9228298
TCGCGTA2651.2369128E-920.7597689
CATGGGG84200.020.644434
TAAGGCG9950.019.359855
GAGTACA79900.018.9519541
TAAGGTG15600.018.228165
GCAGAGT106200.017.9579169
TATACCG2353.5355733E-617.5650925
GTGTAGC22700.017.437231
Produced by FastQC (version 0.11.5)