##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920877_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3914341
Sequences flagged as poor quality	0
Sequence length	98
%GC	48
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.485936713229634	31.0	28.0	33.0	20.0	34.0
2	28.51431824667294	31.0	28.0	34.0	16.0	34.0
3	28.59161963661316	31.0	28.0	34.0	18.0	34.0
4	32.05505830994285	35.0	32.0	37.0	22.0	37.0
5	32.018999622158624	35.0	32.0	37.0	22.0	37.0
6	32.14104008822941	35.0	32.0	37.0	23.0	37.0
7	31.974891814484227	35.0	32.0	37.0	21.0	37.0
8	31.915441705257667	35.0	32.0	37.0	19.0	37.0
9	33.167129537258	37.0	33.0	39.0	19.0	39.0
10-11	32.97170609305628	37.0	32.0	39.0	18.0	39.0
12-13	32.800186289339635	37.0	32.0	39.0	17.0	39.0
14-15	33.55129471346518	38.0	32.0	40.0	17.0	41.0
16-17	33.43974745685161	38.0	32.0	40.0	17.0	41.0
18-19	33.32367862176545	38.0	32.0	40.0	17.0	41.0
20-21	33.227129675212254	38.0	32.0	40.0	16.0	41.0
22-23	33.00843424218789	38.0	32.0	40.0	12.0	41.0
24-25	32.81638186351164	38.0	31.5	40.0	10.5	41.0
26-27	32.859670759394746	38.0	32.0	40.0	10.0	41.0
28-29	32.70558313647176	38.0	31.0	40.0	10.0	41.0
30-31	32.46757053102936	37.5	31.0	40.0	9.0	41.0
32-33	32.22919387963389	37.0	30.5	40.0	8.0	41.0
34-35	31.909704979714338	37.0	30.0	40.0	5.0	41.0
36-37	31.611169032028634	37.0	30.0	40.0	2.0	41.0
38-39	31.420894091751332	37.0	30.0	40.0	2.0	41.0
40-41	31.226259541516697	37.0	30.0	40.0	2.0	41.0
42-43	31.006077523649573	36.0	29.5	40.0	2.0	41.0
44-45	30.73561577798153	36.0	29.0	39.0	2.0	41.0
46-47	30.502550365438267	36.0	28.0	39.0	2.0	40.0
48-49	30.21371324061956	35.5	28.0	39.0	2.0	40.0
50-51	29.0870486245322	34.0	25.5	38.0	2.0	39.5
52-53	29.539375082549018	34.5	27.5	38.5	2.0	39.5
54-55	30.197551133128158	36.0	28.0	39.0	2.0	40.0
56-57	30.158087274460758	35.5	28.0	39.0	2.0	41.0
58-59	29.926171480716675	35.0	28.0	39.0	2.0	40.5
60-61	29.60696564249257	35.0	27.5	39.0	2.0	40.0
62-63	29.223047506591787	35.0	26.5	39.0	2.0	40.0
64-65	28.810678604648906	34.0	26.0	38.0	2.0	40.0
66-67	28.430581546165755	34.0	26.0	38.0	2.0	40.0
68-69	27.991540210727678	34.0	25.5	37.0	2.0	40.0
70-71	27.667357928192764	34.0	25.0	36.5	2.0	39.0
72-73	27.207174592096088	33.0	24.0	36.0	2.0	39.0
74-75	26.769280959425863	33.0	23.0	35.5	2.0	38.5
76-77	26.35001345053995	33.0	22.0	35.0	2.0	37.5
78-79	25.89160538134005	32.0	20.0	35.0	2.0	37.0
80-81	25.439972782136252	32.0	19.0	35.0	2.0	36.5
82-83	24.966852274750718	32.0	17.5	35.0	2.0	36.0
84-85	24.5653843137325	31.5	11.5	34.5	2.0	35.5
86-87	24.10768223821072	31.0	6.0	34.0	2.0	35.0
88-89	23.575901026507402	31.0	2.0	34.0	2.0	35.0
90-91	23.078789379872628	31.0	2.0	34.0	2.0	35.0
92-93	22.542205316297178	30.0	2.0	34.0	2.0	35.0
94-95	21.906048425520414	30.0	2.0	34.0	2.0	35.0
96-97	21.265599241353783	29.5	2.0	34.0	2.0	35.0
98	20.867298224656462	29.0	2.0	34.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	206548.0
3	35432.0
4	25765.0
5	20861.0
6	19292.0
7	19522.0
8	22345.0
9	25265.0
10	27793.0
11	29690.0
12	30655.0
13	30235.0
14	28560.0
15	29795.0
16	31756.0
17	34425.0
18	35551.0
19	35835.0
20	37343.0
21	39868.0
22	43455.0
23	48533.0
24	55302.0
25	63633.0
26	72895.0
27	84453.0
28	97939.0
29	113567.0
30	131656.0
31	154744.0
32	183247.0
33	220793.0
34	274301.0
35	346195.0
36	439638.0
37	487392.0
38	299798.0
39	30262.0
40	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.47353964193586	17.738962903537708	12.720520081927061	25.066977372599368
2	16.02997491187983	19.539424225342323	38.95289678607331	25.477704076704537
3	19.523806238576743	23.300082762314247	28.902215009797843	28.273895989311164
4	13.422929227709385	15.496071385773282	36.29131969294694	34.78967969357039
5	14.576337190613128	36.588129295105674	34.25664104402594	14.578892470255267
6	33.94518973442725	34.67376502884583	17.238323489781624	14.142721746945297
7	29.476218071363157	31.22076955782313	20.813914508110937	18.489097862702774
8	26.701667661390545	34.29250094021846	20.084803616979528	18.921027781411475
9	26.768720456393552	15.747478311163999	19.36555859594246	38.118242636499986
10-11	25.700877874462137	25.144730620045625	27.760483820903698	21.39390768458854
12-13	26.307803542047765	22.48053202252358	27.3979336351466	23.813730800282055
14-15	23.4096138573908	24.146849897980807	24.849838965135916	27.593697279492474
16-17	23.719208619657582	27.046122464725812	25.62828250415227	23.60638641146433
18-19	23.664364634706565	26.48369250271062	26.83953310217176	23.012409760411057
20-21	24.367567351970887	25.681368948255617	26.706576767734358	23.244486932039138
22-23	24.053130607586514	25.53195434067633	26.469547281008605	23.945367770728552
24-25	23.804528509774023	25.70375705642391	26.54732605232027	23.944388381481797
26-27	23.72824177943949	25.885198945176835	26.465111361975413	23.921447913408265
28-29	23.765856359395602	26.098274440064372	26.34459128619648	23.79127791434355
30-31	24.020740404476484	25.91402887379619	26.163602842035065	23.90162787969226
32-33	23.714541345981	25.90165794292631	26.161830632485124	24.22197007860757
34-35	24.080381929780014	25.883274140130556	26.163579344548022	23.872764585541407
36-37	24.185196799110788	25.568128557424597	26.198773278394615	24.047901365069997
38-39	23.960555424636674	25.764976991648403	26.24872225739063	24.025745326324298
40-41	24.156390322687063	25.636371268229237	26.311531143268752	23.89570726581495
42-43	24.046839777630375	25.507933432808628	26.18590932009961	24.259317469461383
44-45	23.96200065056134	25.555465484847765	26.298249538501633	24.18428432608925
46-47	24.00600628256027	25.522160383956756	26.220447808459767	24.251385525023206
48-49	23.794185084038517	25.606501670202526	26.2263093000129	24.373003945746056
50-51	23.71047725845703	25.777207056177748	26.329804697278732	24.18251098808649
52-53	23.865832178195078	25.51532084184235	26.344864526087047	24.27398245387553
54-55	23.525367212918688	25.422046865379517	26.62717459010566	24.425411331596134
56-57	23.512545740883	25.58576040321252	26.56500401026505	24.336689845639434
58-59	23.521202547679195	25.335426683675138	26.557702137539017	24.585668631106646
60-61	23.44564482668864	25.340560514492783	26.741724873723815	24.47206978509476
62-63	23.42833168901756	25.284612847005782	26.783877394069894	24.503178069906763
64-65	23.258271547524224	25.247338933741	26.606312335205228	24.88807718352955
66-67	23.255762611900597	25.291135682339327	26.611358941233775	24.841742764526302
68-69	23.41297202398461	25.140853715714673	26.614498956384956	24.83167530391576
70-71	23.221240649201626	25.015101424771824	26.70106282487617	25.062595101150375
72-73	23.197558274566678	25.01491655574869	26.75323696310976	25.03428820657487
74-75	23.20144906191289	25.092825561776184	26.716431704266675	24.989293672044248
76-77	23.197084603262862	24.90100125435583	26.83742107722838	25.064493065152927
78-79	23.106502675400733	24.950475124765514	26.87684055970319	25.066181640130562
80-81	23.234261626072687	24.888912420515062	26.874572857183697	25.002253096228554
82-83	23.185561406837284	24.82922176813541	26.918928412103437	25.06628841292387
84-85	23.154157178807857	24.7789349728661	26.96973461172176	25.097173236604288
86-87	23.214331491032887	24.71167058027337	26.9904150815705	25.083582847123242
88-89	23.164944592144867	24.523113139379234	27.023740508201442	25.288201760274454
90-91	23.13192234359952	24.58644141155041	27.075831296608357	25.205804948241717
92-93	23.27851725466242	24.335685401814015	27.1678971815558	25.217900161967766
94-95	23.25397565080141	24.20970611714419	27.196596183718803	25.339722048335595
96-97	23.41252768452031	24.04115026073062	27.19722403552234	25.349098019226734
98	23.392133868728944	23.94015205549764	27.14703911221823	25.52067496355519
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	50.0
1	51.0
2	80.5
3	134.5
4	149.0
5	182.5
6	301.5
7	530.5
8	812.0
9	964.0
10	1018.5
11	1123.0
12	1294.0
13	1485.5
14	1674.5
15	1818.5
16	1945.5
17	2043.0
18	2035.5
19	2004.5
20	2005.5
21	2050.0
22	2160.0
23	2432.5
24	2867.0
25	3473.0
26	4393.5
27	5791.0
28	7898.0
29	11114.5
30	16283.5
31	23862.5
32	32164.5
33	40859.0
34	51039.5
35	62496.5
36	75527.0
37	88584.0
38	99216.5
39	109349.5
40	119879.5
41	129201.0
42	138593.0
43	148412.0
44	156818.5
45	164752.0
46	172128.5
47	177016.5
48	179867.0
49	179692.0
50	178491.0
51	177697.0
52	174604.5
53	170213.0
54	163106.0
55	154335.5
56	141846.5
57	126640.0
58	114243.5
59	99211.5
60	82042.5
61	66832.5
62	53872.0
63	41446.5
64	30385.0
65	22924.0
66	17322.5
67	12919.5
68	9592.0
69	7098.0
70	5226.0
71	3760.0
72	2735.5
73	2060.0
74	1615.5
75	1154.5
76	730.5
77	453.0
78	264.0
79	137.5
80	84.0
81	52.5
82	29.0
83	17.0
84	14.0
85	11.5
86	8.0
87	7.5
88	7.5
89	5.5
90	7.5
91	7.0
92	4.0
93	4.5
94	3.5
95	2.5
96	3.0
97	2.5
98	1.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.38992514959733965
2	0.023886523938512257
3	0.018496089124580613
4	0.015839192344254117
5	0.022353698872939278
6	0.024269730204905502
7	0.0156603627532706
8	0.009120309140159225
9	0.0
10-11	0.0
12-13	0.0056459056581938056
14-15	0.0032317061799163643
16-17	0.005569264404915157
18-19	0.03815457059055407
20-21	0.08006456259176194
22-23	0.09968472343109608
24-25	0.0656176863487366
26-27	0.07598980262578042
28-29	0.05892435022906794
30-31	0.05298465309997265
32-33	0.02009278190121913
34-35	0.3427013640354788
36-37	0.5249414907898928
38-39	0.4802596401284405
40-41	0.490235776596878
42-43	0.4400740763260022
44-45	0.41307080808749164
46-47	0.41103981487560737
48-49	0.4013957905047108
50-51	0.3978447457694667
52-53	0.4593876721522218
54-55	0.4606650263735326
56-57	0.4302895429907614
58-59	0.4159959492542934
60-61	0.4300212986042861
62-63	0.4103628171383127
64-65	0.4602945936493525
66-67	0.4819074270739315
68-69	0.42386445125756805
70-71	0.3800001072977546
72-73	0.3656298723080079
74-75	0.3477085925830172
76-77	0.3457542406244116
78-79	0.34548599623793635
80-81	0.3615551123420264
82-83	0.356662845674406
84-85	0.3566756192166191
86-87	0.34286742008424914
88-89	0.38625914298217756
90-91	0.3650422893662049
92-93	0.35442747578711203
94-95	0.3609930764846496
96-97	0.3614146033776822
98	0.35569205646620977
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
98	3914341.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.232859043570485
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.70973159999747	44.08794128489207
2	12.472360952712341	14.526024745996605
3	4.915690075045079	8.587640617259359
4	2.410616518031739	5.615083676105797
5	1.3267118518659755	3.862911213057286
6	0.812418824342966	2.8385682529384315
7	0.5380855480644404	2.1933981911673253
8	0.3642436791052872	1.6968760658279767
9	0.2572573405840353	1.3482747406938542
>10	1.127750605032528	11.073456255864784
>50	0.04680745789249369	1.8493448000404575
>100	0.016957442256031418	1.6479738254005811
>500	0.001015045750644974	0.3875238330051633
>1k	3.530593188494049E-4	0.2849824977502039
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.5547084426216314E-5
2	0.0	0.0	0.0	0.0	2.5547084426216314E-5
3	0.0	0.0	0.0	5.109416885243263E-5	5.109416885243263E-5
4	0.0	0.0	0.0	5.109416885243263E-5	5.109416885243263E-5
5	0.0	0.0	0.0	5.109416885243263E-5	5.109416885243263E-5
6	0.0	0.0	0.0	5.109416885243263E-5	5.109416885243263E-5
7	0.0	0.0	0.0	1.532825065572979E-4	5.109416885243263E-5
8	0.0	0.0	0.0	3.065650131145958E-4	5.109416885243263E-5
9	0.0	0.0	0.0	3.5765918196702844E-4	5.109416885243263E-5
10-11	0.0	0.0	0.0	3.5765918196702844E-4	5.109416885243263E-5
12-13	0.0	0.0	0.0	4.7262106188500184E-4	1.7882959098351422E-4
14-15	0.0	0.0	0.0	4.8539460409811E-4	2.2992375983594683E-4
16-17	0.0	0.0	0.0	5.237152307374345E-4	2.8101792868837945E-4
18-19	0.0	0.0	0.0	5.748093995898671E-4	3.4488563975392027E-4
20-21	0.0	0.0	0.0	6.259035684422998E-4	3.5765918196702844E-4
22-23	0.0	0.0	0.0	7.153183639340569E-4	3.5765918196702844E-4
24-25	0.0	0.0	0.0	9.196950393437874E-4	3.5765918196702844E-4
26-27	0.0	0.0	0.0	0.0016605604877040605	4.215268930325692E-4
28-29	0.0	0.0	0.0	0.006118526720078808	4.343004352456774E-4
30-31	0.0	0.0	0.0	0.027399248047117	4.4707397745878555E-4
32-33	0.0	0.0	0.0	0.08057550428028626	4.5984751967189367E-4
34-35	0.0	0.0	0.0	0.1504978743548403	5.109416885243263E-4
36-37	0.0	0.0	0.0	0.2656641309482235	5.109416885243263E-4
38-39	0.0	0.0	0.0	0.4600518963473035	5.109416885243263E-4
40-41	0.0	0.0	0.0	0.6824009456508772	5.109416885243263E-4
42-43	0.0	0.0	0.0	0.9239741759851786	5.237152307374345E-4
44-45	0.0	0.0	0.0	1.214648902586668	5.364887729505427E-4
46-47	0.0	0.0	0.0	1.5585126589635394	5.492623151636508E-4
48-49	0.0	0.0	0.0	1.9186243610354847	5.620358573767589E-4
50-51	0.0	0.0	0.0	2.3102867123738067	6.386771106554079E-4
52-53	0.0	0.0	0.0	2.713636854837123	7.919596172127058E-4
54-55	0.0	0.0	0.0	3.1684260517926264	7.919596172127058E-4
56-57	0.0	0.0	0.0	3.694466067212846	7.919596172127058E-4
58-59	0.0	0.0	0.0	4.248697290297397	7.919596172127058E-4
60-61	0.0	0.0	0.0	4.828335088843819	8.430537860651384E-4
62-63	0.0	0.0	0.0	5.425242716462362	8.686008704913548E-4
64-65	2.5547084426216314E-5	0.0	0.0	6.071571178903422	9.069214971306793E-4
66-67	2.5547084426216314E-5	0.0	0.0	6.800991533440751	9.196950393437873E-4
68-69	2.5547084426216314E-5	0.0	0.0	7.5857213257608365	9.196950393437873E-4
70-71	2.5547084426216314E-5	0.0	0.0	8.400864922090335	9.580156659831119E-4
72-73	2.5547084426216314E-5	0.0	0.0	9.238758197101376	9.7078920819622E-4
74-75	2.5547084426216314E-5	0.0	0.0	10.101508785259128	9.7078920819622E-4
76-77	2.5547084426216314E-5	0.0	0.0	11.04641879693159	9.963362926224363E-4
78-79	2.5547084426216314E-5	0.0	0.0	12.061608837860575	0.0010218833770486526
80-81	2.5547084426216314E-5	0.0	0.0	13.100442705426023	0.0010218833770486526
82-83	2.5547084426216314E-5	0.0	0.0	14.151692456022609	0.0010729775459010853
84-85	2.5547084426216314E-5	0.0	0.0	15.224516719417139	0.0010729775459010853
86	2.5547084426216314E-5	0.0	0.0	16.048908360308925	0.0010729775459010853
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	5170	0.0	40.061466	1
GGTATCA	2915	0.0	38.210407	1
TCAACGC	6430	0.0	31.669977	4
ATCAACG	6505	0.0	31.44625	3
CAACGCA	6530	0.0	31.184988	5
TATCAAC	6785	0.0	30.081715	2
AACGCAG	7020	0.0	29.459572	6
GTACATG	10610	0.0	26.591934	1
TACATGG	11130	0.0	24.684486	2
ACGCAGA	8275	0.0	24.65542	7
ACATGGG	11570	0.0	23.26945	3
CGCAGAG	8780	0.0	22.922829	8
TCGCGTA	265	1.2369128E-9	20.759768	9
CATGGGG	8420	0.0	20.64443	4
TAAGGCG	995	0.0	19.35985	5
GAGTACA	7990	0.0	18.951954	1
TAAGGTG	1560	0.0	18.22816	5
GCAGAGT	10620	0.0	17.957916	9
TATACCG	235	3.5355733E-6	17.565092	5
GTGTAGC	2270	0.0	17.43723	1
>>END_MODULE