FastQCFastQC Report
Mon 15 Aug 2016
SRR2920877_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920877_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3914341
Sequences flagged as poor quality0
Sequence length101
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA52650.042.360391
GGTATCA29000.041.978551
TCAACGC64250.033.763274
ATCAACG64700.033.7485433
TATCAAC66300.033.004432
CAACGCA66550.032.5209125
AACGCAG71850.030.3862426
GTACATG105800.026.6535191
TACATGG109500.025.228632
ACGCAGA85500.024.9244827
TATTACG1151.2887293E-424.7649922
CGCAGAG88350.024.0111838
ACATGGG113800.023.6923183
CATGGGG85000.020.0482754
GAGTACA79050.019.6110671
GCAGAGT107150.019.133939
AGTACAT77200.018.6913682
TAAGGCG9800.017.9193215
AGAGTAC86650.017.52521910-11
TAAGGTG16350.017.4172445
Produced by FastQC (version 0.11.5)