##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920877_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3914341
Sequences flagged as poor quality	0
Sequence length	101
%GC	48
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.27333208833875	31.0	30.0	34.0	27.0	34.0
2	30.299419749071426	31.0	30.0	34.0	26.0	34.0
3	30.43685335539239	31.0	30.0	34.0	27.0	34.0
4	34.076202865309895	35.0	35.0	37.0	30.0	37.0
5	33.602968673398664	35.0	35.0	37.0	28.0	37.0
6	33.54649122291594	35.0	35.0	37.0	28.0	37.0
7	33.36741893463038	35.0	35.0	37.0	27.0	37.0
8	33.29813805184576	35.0	35.0	37.0	27.0	37.0
9	34.63879462724377	38.0	35.0	39.0	27.0	39.0
10-11	34.45010731052813	38.0	34.0	39.0	27.0	39.0
12-13	34.27394176950858	37.0	34.0	39.0	27.0	39.0
14-15	35.19013826337562	38.0	34.0	40.0	26.0	41.0
16-17	35.06788524556241	38.0	34.0	40.0	25.5	41.0
18-19	34.97878135808811	38.5	34.0	40.0	25.0	41.0
20-21	34.86727510454506	38.5	34.0	40.0	25.0	41.0
22-23	34.72232861163603	38.0	34.0	40.0	24.5	41.0
24-25	34.55549120528845	38.0	33.5	40.0	24.0	41.0
26-27	34.37841772088839	38.0	33.0	40.0	23.5	41.0
28-29	34.32697662262946	38.0	33.0	40.0	23.5	41.0
30-31	34.32276160916997	38.0	33.0	40.0	23.0	41.0
32-33	34.187234326288895	38.0	33.0	40.0	23.0	41.0
34-35	34.02851744393245	38.0	33.0	40.0	22.0	41.0
36-37	33.84690117697973	38.0	33.0	40.0	20.0	41.0
38-39	33.67309708070911	38.0	33.0	40.0	19.0	41.0
40-41	33.45844562341401	38.0	32.0	40.0	18.0	41.0
42-43	33.48403996994641	38.0	32.5	40.0	18.0	41.0
44-45	33.3449235771743	38.0	32.0	40.0	16.5	41.0
46-47	33.11580736067706	38.0	32.0	40.0	15.0	41.0
48-49	32.872080383390205	37.5	31.5	40.0	13.5	41.0
50-51	32.632433658692484	37.0	31.0	40.0	10.5	41.0
52-53	32.380972046124754	37.0	31.0	40.0	9.0	41.0
54-55	32.107520780637145	37.0	31.0	40.0	8.0	41.0
56-57	31.795326467469238	36.0	30.0	39.5	7.0	41.0
58-59	31.47247723179968	36.0	30.0	39.0	4.0	41.0
60-61	31.15345175599162	35.5	29.5	39.0	2.0	40.0
62-63	30.838770306419395	35.0	29.0	39.0	2.0	40.0
64-65	30.4434101423458	35.0	29.0	38.5	2.0	40.0
66-67	30.02308319586873	34.5	28.0	38.0	2.0	40.0
68-69	29.589917051171575	34.0	28.0	37.0	2.0	40.0
70-71	29.16307738646173	34.0	28.0	37.0	2.0	39.0
72-73	28.727500235671855	34.0	26.5	36.0	2.0	39.0
74-75	28.287779986465154	34.0	26.0	36.0	2.0	38.5
76-77	26.80972953046247	31.5	25.0	34.5	2.0	36.5
78-79	27.644494437250103	33.0	26.0	35.0	2.0	37.0
80-81	27.67037478339266	33.0	26.0	35.0	2.0	37.0
82-83	27.332608349655793	33.0	26.0	35.0	2.0	36.0
84-85	27.01452109052328	33.0	25.5	35.0	2.0	36.0
86-87	26.4799217799369	33.0	25.0	35.0	2.0	35.0
88-89	26.1165590836363	32.5	24.0	35.0	2.0	35.0
90-91	25.7547691935884	32.0	23.0	35.0	2.0	35.0
92-93	25.44928788779516	32.0	20.0	35.0	2.0	35.0
94-95	25.073231739391126	32.0	19.0	34.0	2.0	35.0
96-97	24.551446718617512	32.0	14.0	34.0	2.0	35.0
98-99	23.9630162522887	32.0	2.0	34.0	2.0	35.0
100-101	21.80273639930706	28.0	2.0	33.0	2.0	34.5
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	91629.0
3	41556.0
4	21805.0
5	15402.0
6	13167.0
7	13032.0
8	14543.0
9	16870.0
10	19064.0
11	21687.0
12	23532.0
13	25975.0
14	27920.0
15	29694.0
16	31049.0
17	32189.0
18	32090.0
19	30961.0
20	31090.0
21	32129.0
22	35417.0
23	40012.0
24	47171.0
25	55810.0
26	65593.0
27	78553.0
28	92628.0
29	109084.0
30	128642.0
31	152898.0
32	183383.0
33	223469.0
34	282383.0
35	366633.0
36	495671.0
37	598585.0
38	365481.0
39	27541.0
40	3.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.419181405458644	19.142294836246027	13.434294026389942	25.004229731905387
2	16.13474656398101	20.622424055543448	37.94815014839024	25.294679232085297
3	19.25228362857217	24.114903098928238	29.297924262047705	27.33488901045189
4	13.796610923016427	16.472597410859855	35.94092098004783	33.78987068607589
5	14.553473156883005	36.858277662467465	34.22703084968202	14.361218330967512
6	33.809221301203735	35.26509374031371	17.032175632531015	13.893509325951545
7	29.34063794607143	31.428689843820155	20.880544561028643	18.350127649079774
8	26.62587219853501	34.66763285592568	20.014927675208984	18.691567270330324
9	26.60309504250317	15.688195279994947	19.370558541862433	38.33815113563945
10-11	25.70489371429302	25.320097573763782	27.820850477478427	21.154158234464767
12-13	26.261764541891974	22.583777042652876	27.53106135746662	23.623397057988534
14-15	23.32630007710195	24.311053994767196	24.859566134327736	27.50307979380312
16-17	23.713517252803886	27.20108228826662	25.654687768424665	23.430712690504823
18-19	23.640156933732698	26.566812529642398	26.8723066165031	22.920723920121805
20-21	24.356441109206976	25.80835642935301	26.638714189455897	23.196488271984112
22-23	24.043793741037334	25.65356746860246	26.495433219326287	23.80720557103392
24-25	23.793664317248858	25.83337103751425	26.538464782889104	23.834499862347787
26-27	23.692903023272105	26.019729929683145	26.474146168873965	23.81322087817079
28-29	23.786857258231464	26.162807042653085	26.369580829753136	23.68075486936231
30-31	23.972577973784865	25.969536845728484	26.196822901500898	23.861062278985745
32-33	23.724169144180777	25.955688797053227	26.20595531057338	24.11418674819261
34-35	24.049254058755672	25.968255639291787	26.18762194025852	23.794868361694018
36-37	24.19829621135412	25.700338905090604	26.18017229538725	23.921192588168026
38-39	23.93787523680504	25.853935659561937	26.293914509705907	23.91427459392711
40-41	24.142999495696042	25.676839926870176	26.32913863682564	23.851021940608145
42-43	24.001639836215674	25.63285273118452	26.202068549395214	24.163438883204595
44-45	23.9478965853499	25.59317611727932	26.32755178807622	24.13137550929456
46-47	23.954324378233547	25.560809147084534	26.269884278866932	24.214982195814986
48-49	23.691156594677043	25.68921995201827	26.30414232024098	24.315481133063702
50-51	23.676549232994535	25.789943958789152	26.33400336814499	24.199503440071318
52-53	23.893946733461895	25.523396214460103	26.29806655609072	24.28459049598728
54-55	23.52628484659412	25.5071690590784	26.47342132380201	24.49312477052547
56-57	23.43682076750693	25.61413315834271	26.408169715014722	24.540876359135634
58-59	23.448383692695117	25.43379087010334	26.400365133529093	24.71746030367245
60-61	23.40249677295628	25.457736810964036	26.506591499513917	24.633174916565764
62-63	23.348298332320066	25.464072313355146	26.43991220545283	24.747717148871953
64-65	23.137743438794896	25.413392297003984	26.317647826796218	25.131216437404902
66-67	23.128089607367166	25.470338491389867	26.2568386035882	25.144733297654774
68-69	23.313963746269916	25.377342569366135	26.207128008039337	25.10156567632461
70-71	23.090570237555436	25.25643831534991	26.211653073216052	25.441338373878597
72-73	23.06151012270025	25.302852901552775	26.14718866987532	25.488448305871653
74-75	23.003090568460554	25.44019569764846	25.96106911627947	25.595644617611512
76-77	22.97982815145835	25.29044918632736	26.181668421725956	25.548054240488327
78-79	22.904938017506204	25.422857778397084	26.092335187417625	25.57986901667909
80-81	22.91463717981165	25.474713078201738	25.970784076822333	25.639865665164272
82-83	22.83173425106343	25.444091695166883	25.98847761397669	25.735696439793
84-85	22.679308682705148	25.365986211009346	25.991635440416914	25.963069665868588
86-87	22.660453363789326	25.4126871313904	25.94184167143876	25.985017833381512
88-89	22.55176412625579	25.371790252700016	25.835140671608748	26.241304949435445
90-91	22.46765957861011	25.38695542808313	25.833414874928856	26.311970118377904
92-93	22.485281660964944	25.236144610437023	25.868692213662804	26.40988151493523
94-95	22.322429140716345	25.205212848972973	25.864004182623688	26.608353827686994
96-97	22.342444788180806	25.10398422349739	25.845302542004056	26.708268446317753
98-99	22.18031080617075	25.159010456265996	25.76982367825113	26.890855059312123
100-101	22.271214167215213	25.082754544394287	25.7970118816786	26.849019406711903
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	58.0
1	51.0
2	69.0
3	97.5
4	82.5
5	88.5
6	162.0
7	321.0
8	493.0
9	536.5
10	446.5
11	373.5
12	405.0
13	447.0
14	515.0
15	586.0
16	652.0
17	734.5
18	800.5
19	890.5
20	1055.0
21	1259.5
22	1480.0
23	1775.0
24	2150.5
25	2720.0
26	3589.5
27	4735.0
28	6284.0
29	8641.5
30	12681.0
31	18607.5
32	26509.0
33	34267.5
34	42517.5
35	52505.5
36	64008.5
37	76311.0
38	87899.0
39	98111.5
40	107794.5
41	118207.5
42	128182.5
43	137609.5
44	147096.0
45	156515.0
46	164319.5
47	170474.0
48	175849.5
49	178300.5
50	177606.0
51	176099.0
52	173643.0
53	170371.0
54	165497.0
55	156135.0
56	143124.5
57	128487.5
58	115482.5
59	101348.5
60	83675.5
61	67903.5
62	54495.5
63	41651.5
64	30599.0
65	22950.5
66	17430.0
67	13051.5
68	9745.5
69	7162.5
70	5184.0
71	3905.0
72	2847.0
73	2111.0
74	1613.0
75	1180.0
76	806.0
77	467.5
78	260.5
79	130.0
80	66.5
81	39.0
82	19.0
83	6.5
84	3.0
85	2.0
86	1.5
87	2.0
88	1.0
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.19093890900154076
2	0.0
3	0.012875730550813022
4	0.013591048914747082
5	0.006769977372947324
6	0.10671017164830555
7	0.1425016369294346
8	0.12323913527206752
9	0.14239944859172976
10-11	0.11441261760280978
12-13	0.1149363328335472
14-15	0.11706951438313626
16-17	0.06716328495652268
18-19	0.08011565676061437
20-21	0.03856332394137353
22-23	0.08495682925938236
24-25	0.058962670855707264
26-27	0.06690781411226053
28-29	0.04115635301063448
30-31	0.02530438712416726
32-33	0.033530548309408915
34-35	0.02032270566105508
36-37	0.025636499221708072
38-39	0.03351777476719581
40-41	0.026224082163511048
42-43	0.013680463710238837
44-45	0.0277185866024447
46-47	0.03465462002416243
48-49	0.008967026633601927
50-51	0.01568590983769682
52-53	0.0073831073991765155
54-55	0.012977918888517887
56-57	0.005786414622537995
58-59	0.0039981187127028535
60-61	0.023746014974168065
62-63	0.010998019845486123
64-65	0.0074725221946682725
66-67	0.04156510636145395
68-69	0.09122863848601846
70-71	0.022366472415152383
72-73	0.045933657798336934
74-75	0.08711555789339763
76-77	0.25328656854372167
78-79	0.08062659844913869
80-81	0.01756362054302372
82-83	0.012300921151223157
84-85	0.014472423327451541
86-87	0.26994326758961473
88-89	0.6236681985550058
90-91	0.26576631928592837
92-93	0.3516556171268676
94-95	0.0013156748479501402
96-97	0.0042280424725388
98-99	0.0033977622286867704
100-101	0.005249925849587452
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3914341.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.42459956174785
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.32923454802902	40.64243460993994
2	13.499233661870294	14.96379240199256
3	5.442284872726185	9.049093793154201
4	2.6847088612187266	5.95195654291696
5	1.4597139935352392	4.045203178318521
6	0.9374291737321375	3.117398194296233
7	0.5994141473905816	2.325560236353864
8	0.39657045638321897	1.7583806994447604
9	0.30151104198398826	1.5040015888867104
>10	1.2745030855001198	12.001354246996991
>50	0.05365454089811693	2.029029689795855
>100	0.020399102685757033	1.9350898032425492
>500	9.258717829781554E-4	0.35726249091541923
>1k	4.166422638106741E-4	0.3194425237455175
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	5.109416885243263E-5
2	0.0	0.0	0.0	0.0	5.109416885243263E-5
3	0.0	0.0	0.0	0.0	5.109416885243263E-5
4	0.0	0.0	0.0	2.5547084426216314E-5	5.109416885243263E-5
5	2.5547084426216314E-5	0.0	0.0	2.5547084426216314E-5	5.109416885243263E-5
6	2.5547084426216314E-5	0.0	0.0	2.5547084426216314E-5	5.109416885243263E-5
7	2.5547084426216314E-5	0.0	0.0	1.2773542213108158E-4	7.664125327864895E-5
8	2.5547084426216314E-5	0.0	0.0	2.8101792868837945E-4	7.664125327864895E-5
9	2.5547084426216314E-5	0.0	0.0	3.321120975408121E-4	7.664125327864895E-5
10-11	2.5547084426216314E-5	0.0	0.0	3.5765918196702844E-4	7.664125327864895E-5
12-13	2.5547084426216314E-5	0.0	0.0	4.343004352456774E-4	1.6605604877040605E-4
14-15	2.5547084426216314E-5	0.0	0.0	4.5984751967189367E-4	2.171502176228387E-4
16-17	2.5547084426216314E-5	0.0	0.0	5.237152307374345E-4	2.5547084426216316E-4
18-19	2.5547084426216314E-5	0.0	0.0	5.492623151636508E-4	2.937914709014876E-4
20-21	2.5547084426216314E-5	0.0	0.0	5.875829418029752E-4	3.193385553277039E-4
22-23	2.5547084426216314E-5	0.0	0.0	6.897712795078405E-4	3.5765918196702844E-4
24-25	2.5547084426216314E-5	0.0	0.0	9.196950393437873E-4	3.8320626639324473E-4
26-27	2.5547084426216314E-5	0.0	0.0	0.0016477869454909525	4.0875335081946103E-4
28-29	2.5547084426216314E-5	0.0	0.0	0.006220715057783673	4.0875335081946103E-4
30-31	2.5547084426216314E-5	0.0	0.0	0.027705813060231593	4.215268930325692E-4
32-33	2.5547084426216314E-5	0.0	0.0	0.08102257825774505	4.8539460409811E-4
34-35	2.5547084426216314E-5	0.0	0.0	0.15154530481631517	4.8539460409811E-4
36-37	2.5547084426216314E-5	0.0	0.0	0.26788672729330426	4.8539460409811E-4
38-39	2.5547084426216314E-5	0.0	0.0	0.46269601958541684	5.364887729505427E-4
40-41	2.5547084426216314E-5	0.0	0.0	0.6892475642771031	5.364887729505427E-4
42-43	2.5547084426216314E-5	0.0	0.0	0.9352148931327138	5.364887729505427E-4
44-45	2.5547084426216314E-5	0.0	0.0	1.2295173057227258	5.492623151636508E-4
46-47	2.5547084426216314E-5	0.0	0.0	1.5764850328573827	5.620358573767589E-4
48-49	2.5547084426216314E-5	0.0	0.0	1.9372098649555571	5.875829418029752E-4
50-51	2.5547084426216314E-5	0.0	0.0	2.327875880001257	6.131300262291916E-4
52-53	2.5547084426216314E-5	0.0	0.0	2.729437726554738	6.642241950816242E-4
54-55	2.5547084426216314E-5	0.0	0.0	3.180726972943849	6.897712795078405E-4
56-57	2.5547084426216314E-5	0.0	0.0	3.703445867388661	7.025448217209487E-4
58-59	2.5547084426216314E-5	0.0	0.0	4.255646097261327	7.153183639340569E-4
60-61	2.5547084426216314E-5	0.0	0.0	4.832997431751602	7.153183639340569E-4
62-63	2.5547084426216314E-5	0.0	0.0	5.427107653625477	7.408654483602731E-4
64-65	2.5547084426216314E-5	0.0	0.0	6.069604053402603	7.664125327864895E-4
66-67	2.5547084426216314E-5	0.0	0.0	6.795090156938294	7.791860749995976E-4
68-69	2.5547084426216314E-5	0.0	0.0	7.575655774496908	8.047331594258139E-4
70-71	2.5547084426216314E-5	0.0	0.0	8.386980081704685	8.175067016389221E-4
72-73	2.5547084426216314E-5	0.0	0.0	9.223519361241138	9.069214971306793E-4
74-75	2.5547084426216314E-5	0.0	0.0	10.088824657841512	9.7078920819622E-4
76-77	2.5547084426216314E-5	0.0	0.0	11.036634263596351	9.83562750409328E-4
78-79	2.5547084426216314E-5	0.0	0.0	12.052654584769186	9.963362926224363E-4
80-81	2.5547084426216314E-5	0.0	0.0	13.091079698983814	9.963362926224363E-4
82-83	2.5547084426216314E-5	0.0	0.0	14.143862274645976	0.0010985246303273017
84-85	2.5547084426216314E-5	0.0	0.0	15.222332443698697	0.0010985246303273017
86-87	2.5547084426216314E-5	0.0	0.0	16.337896979338282	0.001136845256966626
88-89	2.5547084426216314E-5	0.0	0.0	17.52068866764546	0.0011879394258190587
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	5265	0.0	42.36039	1
GGTATCA	2900	0.0	41.97855	1
TCAACGC	6425	0.0	33.76327	4
ATCAACG	6470	0.0	33.748543	3
TATCAAC	6630	0.0	33.00443	2
CAACGCA	6655	0.0	32.520912	5
AACGCAG	7185	0.0	30.386242	6
GTACATG	10580	0.0	26.653519	1
TACATGG	10950	0.0	25.22863	2
ACGCAGA	8550	0.0	24.924482	7
TATTACG	115	1.2887293E-4	24.764992	2
CGCAGAG	8835	0.0	24.011183	8
ACATGGG	11380	0.0	23.692318	3
CATGGGG	8500	0.0	20.048275	4
GAGTACA	7905	0.0	19.611067	1
GCAGAGT	10715	0.0	19.13393	9
AGTACAT	7720	0.0	18.691368	2
TAAGGCG	980	0.0	17.919321	5
AGAGTAC	8665	0.0	17.525219	10-11
TAAGGTG	1635	0.0	17.417244	5
>>END_MODULE