FastQCFastQC Report
Mon 15 Aug 2016
SRR2920876_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920876_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3057296
Sequences flagged as poor quality0
Sequence length98
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA27000.046.2016751
GTATCAA48300.042.219081
TCAACGC58700.034.688134
CAACGCA60600.033.5248685
ATCAACG60350.033.5117653
TATCAAC64100.031.4827942
AACGCAG65550.031.1280736
GTACATG103950.028.6062051
TACATGG105050.027.4620692
ACATGGG106800.025.892983
ACGCAGA79050.024.9375277
CGCAGAG82050.023.856168
CATGGGG76000.022.2059924
GCAGAGT93950.020.004999
TAAGGTG14500.019.2929135
GAGTACA73200.018.1735151
AGAGTAC75500.017.51747310-11
AGTACAT75050.016.928082
CAGAGTA84250.016.81361610-11
GTAAGGT12100.016.676444
Produced by FastQC (version 0.11.5)