##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920876_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3057296
Sequences flagged as poor quality	0
Sequence length	98
%GC	48
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.516002703042165	31.0	29.0	33.0	20.0	34.0
2	28.54128681030558	31.0	28.0	34.0	16.0	34.0
3	28.612894531638414	31.0	28.0	34.0	18.0	34.0
4	32.07786226783406	35.0	32.0	37.0	22.0	37.0
5	32.03659442854077	35.0	32.0	37.0	22.0	37.0
6	32.163969075941615	35.0	32.0	37.0	23.0	37.0
7	31.99664180373768	35.0	32.0	37.0	21.0	37.0
8	31.93218549986655	35.0	32.0	37.0	19.0	37.0
9	33.18340618638169	37.0	33.0	39.0	19.0	39.0
10-11	32.99615722520816	37.0	32.0	39.0	18.0	39.0
12-13	32.82425466817737	37.0	32.0	39.0	17.0	39.0
14-15	33.58075894515939	38.0	32.0	40.0	17.0	41.0
16-17	33.46886987717251	38.0	32.0	40.0	17.0	41.0
18-19	33.353836854527664	38.0	32.0	40.0	17.0	41.0
20-21	33.25771335192928	38.0	32.0	40.0	16.0	41.0
22-23	33.03510618533501	38.0	32.0	40.0	11.5	41.0
24-25	32.84891469455361	38.0	32.0	40.0	10.0	41.0
26-27	32.87190445413202	38.0	32.0	40.0	10.0	41.0
28-29	32.71014026773986	38.0	31.5	40.0	9.5	41.0
30-31	32.46729283000403	38.0	31.0	40.0	9.0	41.0
32-33	32.23044072278249	37.0	30.5	40.0	8.0	41.0
34-35	31.91118082776414	37.0	30.0	40.0	5.0	41.0
36-37	31.60891912330373	37.0	30.0	40.0	2.0	41.0
38-39	31.41359930474511	37.0	30.0	40.0	2.0	41.0
40-41	31.209607280420343	37.0	30.0	40.0	2.0	41.0
42-43	30.97660383554618	36.0	29.5	40.0	2.0	41.0
44-45	30.697662738576835	36.0	29.0	39.0	2.0	41.0
46-47	30.448137013884164	36.0	28.0	39.0	2.0	40.0
48-49	30.145524836325954	35.5	28.0	39.0	2.0	40.0
50-51	28.99131928998697	34.0	25.5	38.0	2.0	39.5
52-53	29.43751357408638	34.5	27.5	38.5	2.0	39.5
54-55	30.08060930312276	35.0	28.0	39.0	2.0	40.0
56-57	30.0281992649714	35.0	28.0	39.0	2.0	40.5
58-59	29.77601743501447	35.0	28.0	39.0	2.0	40.0
60-61	29.444446007190667	35.0	27.0	39.0	2.0	40.0
62-63	29.045957931453152	34.5	26.0	38.5	2.0	40.0
64-65	28.62069407083907	34.0	26.0	38.0	2.0	40.0
66-67	28.229812716858298	34.0	26.0	37.5	2.0	40.0
68-69	27.780948099235403	34.0	25.0	37.0	2.0	39.0
70-71	27.453954572929803	33.5	24.5	36.0	2.0	39.0
72-73	26.993089808772197	33.0	23.5	36.0	2.0	39.0
74-75	26.563713817700346	33.0	22.5	35.0	2.0	38.0
76-77	26.155302430644596	32.5	21.5	35.0	2.0	37.0
78-79	25.708882293372966	32.0	20.0	35.0	2.0	37.0
80-81	25.281471273962353	32.0	18.5	35.0	2.0	36.0
82-83	24.82448477347303	32.0	16.0	35.0	2.0	36.0
84-85	24.440755654670006	31.0	9.0	34.5	2.0	35.0
86-87	24.003050898571807	31.0	4.5	34.0	2.0	35.0
88-89	23.48835114427913	31.0	2.0	34.0	2.0	35.0
90-91	23.004393261234764	31.0	2.0	34.0	2.0	35.0
92-93	22.47982073047556	30.0	2.0	34.0	2.0	35.0
94-95	21.85908937178474	30.0	2.0	34.0	2.0	35.0
96-97	21.225841724190264	29.0	2.0	34.0	2.0	35.0
98	20.83022808390159	29.0	2.0	34.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	165143.0
3	27536.0
4	20219.0
5	16086.0
6	15391.0
7	15345.0
8	17597.0
9	20066.0
10	21973.0
11	23396.0
12	24213.0
13	24162.0
14	22565.0
15	23569.0
16	25440.0
17	26941.0
18	27863.0
19	28003.0
20	28768.0
21	30495.0
22	33312.0
23	37261.0
24	42264.0
25	48837.0
26	57033.0
27	65874.0
28	76143.0
29	87851.0
30	102806.0
31	119968.0
32	143645.0
33	174286.0
34	218041.0
35	275382.0
36	350908.0
37	381191.0
38	217781.0
39	19940.0
40	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.47672089635943	17.599662985246738	12.924863834744565	24.998752283649267
2	16.18129584244089	19.275884508211767	38.761974775963196	25.780844873384158
3	19.538347086673525	23.274845651144105	28.671134212186168	28.515673049996202
4	13.60208688620104	15.195385505706453	35.744665259750136	35.45786234834237
5	14.805773151625711	36.606303931909366	33.64415474286731	14.943768173597608
6	34.3118266553686	34.183676473956105	16.87011912045201	14.63437775022329
7	29.861012515612618	31.1940636386417	20.398963367837577	18.545960477908103
8	27.38353972249991	33.849011844260794	19.650782726116518	19.11666570712278
9	27.24355116416598	15.515017191662176	19.033158712797192	38.20827293137465
10-11	26.13066025184677	24.887976971804	27.11028423650903	21.871078539840195
12-13	26.669522277279135	22.22433748934257	27.089310121438686	24.01683011193961
14-15	23.86804010811902	23.79274211763005	24.456473296607843	27.88274447764309
16-17	24.031676549710053	26.833329626101705	25.192307189063577	23.94268663512467
18-19	24.11617558038709	25.988204113018014	26.579601786561526	23.316018520033374
20-21	24.782883737373655	25.281202406974433	26.293454145794954	23.642459709856958
22-23	24.556953902251642	25.055123439015038	26.15507116812907	24.232851490604254
24-25	24.28491450885694	25.30525069988657	26.148354843107093	24.261479948149397
26-27	24.01089623389587	25.546166031724056	26.10410374728253	24.338833987097548
28-29	24.17604841313361	25.610216477673546	26.07890698010446	24.134828129088383
30-31	24.330846894968257	25.483983343311923	25.83102222249857	24.354147539221252
32-33	24.10229063698961	25.532648549530414	25.785520740214267	24.579540073265704
34-35	24.50267192765242	25.524707843808127	25.81458717425497	24.158033054284477
36-37	24.609523471335024	25.232572897062283	25.79680095955431	24.361102672048375
38-39	24.34322642090134	25.374003996584122	25.813723555411975	24.469046027102564
40-41	24.534969290471352	25.23727699971833	25.971991831827413	24.25576187798291
42-43	24.394685919638356	25.15311989432678	25.81140937646133	24.640784809573532
44-45	24.232375747164014	25.240641781479084	25.931330780784652	24.595651690572247
46-47	24.472899269536033	25.24139301293193	25.758440319443544	24.527267398088494
48-49	24.268401327561346	25.30316558428232	25.691963604039426	24.73646948411691
50-51	24.11702548246726	25.515697835546735	25.86126956565141	24.5060071163346
52-53	24.342456963044718	25.25005018910862	25.939160614676116	24.468332233170546
54-55	23.95306451157139	25.210730965541124	26.119911784439655	24.71629273844783
56-57	23.94591293882383	25.316089801956913	26.10054960143057	24.63744765778869
58-59	23.918560648046654	24.992639192339176	26.174621511621705	24.914178647992465
60-61	23.82573626238628	25.01196460251406	26.34674452773406	24.8155546073656
62-63	23.842179007392236	25.098903307515762	26.339256612313978	24.719661072778024
64-65	23.734689096537178	25.01832593450766	26.25929809124426	24.9876868777109
66-67	23.5928094711644	25.032814781999424	26.296997274910645	25.07737847192553
68-69	23.84647925858315	24.812121693839405	26.29815115462924	25.043247892948205
70-71	23.60337905428897	24.68654186013452	26.41318397171843	25.29689511385808
72-73	23.550512042581722	24.785930174444896	26.3817763885139	25.281781394459486
74-75	23.670341734543424	24.886125408511813	26.37449918071997	25.069033676224795
76-77	23.648788242836837	24.686937913024444	26.447332168346836	25.216941675791883
78-79	23.60998634859668	24.67357819214986	26.497244201927867	25.21919125732559
80-81	23.62601751178046	24.689611413365036	26.50675118871439	25.177619886140114
82-83	23.655048931033747	24.591541159344654	26.55483837085834	25.19857153876326
84-85	23.576998671316186	24.57603892045753	26.63216727761614	25.214795130610145
86-87	23.666299316584443	24.636517048631987	26.582712185695794	25.114471449087773
88-89	23.528620886141095	24.378770922757614	26.61591627564449	25.4766919154568
90-91	23.48684701837027	24.46680262115884	26.680524724158733	25.365825636312163
92-93	23.722405645746537	24.26268174273464	26.6979695623076	25.316943049211226
94-95	23.558217666107	24.139089531931376	26.823668821630385	25.479023980331238
96-97	23.743835912760872	23.888937943729758	26.878587955858524	25.488638187650853
98	23.779623392604464	23.787862028128867	26.80333392305771	25.62918065620896
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	89.0
1	75.0
2	103.5
3	149.5
4	142.0
5	162.0
6	278.0
7	528.0
8	832.5
9	946.0
10	917.0
11	934.5
12	1021.0
13	1174.0
14	1338.5
15	1492.0
16	1624.5
17	1717.0
18	1740.0
19	1721.0
20	1661.0
21	1627.5
22	1747.5
23	1985.5
24	2313.0
25	2761.0
26	3472.0
27	4494.0
28	6068.0
29	8470.5
30	12038.5
31	17345.0
32	22664.5
33	27701.0
34	34292.5
35	42416.5
36	51414.5
37	59657.5
38	67487.5
39	74951.5
40	81480.0
41	88353.0
42	96791.0
43	106382.0
44	115257.0
45	123999.5
46	131461.5
47	136580.0
48	140962.5
49	143032.5
50	143007.0
51	142536.5
52	141611.0
53	141328.0
54	138334.0
55	132258.5
56	121905.0
57	109984.0
58	99982.5
59	86457.0
60	71651.5
61	58720.0
62	48028.0
63	37309.5
64	26950.5
65	19915.5
66	14923.0
67	11088.0
68	8111.5
69	5910.5
70	4224.5
71	2963.0
72	2163.5
73	1600.5
74	1175.5
75	857.5
76	576.0
77	355.0
78	218.5
79	114.5
80	63.0
81	42.0
82	31.0
83	21.0
84	15.0
85	13.0
86	11.5
87	7.0
88	4.5
89	6.5
90	4.5
91	2.0
92	3.5
93	3.0
94	2.0
95	2.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3837377865931202
2	0.025087528325683874
3	0.01910184686075539
4	0.01612536044923357
5	0.021718538211543795
6	0.023746474008404815
7	0.015503896253421325
8	0.009420088862838273
9	0.0
10-11	1.6354320942427554E-5
12-13	0.005118902454979825
14-15	0.0035815962863916346
16-17	0.0068033975120498635
18-19	0.037484103600043964
20-21	0.07926939360794637
22-23	0.09860020096189574
24-25	0.0647467566110707
26-27	0.0750826874466849
28-29	0.05761627268017228
30-31	0.051892260350322635
32-33	0.02006675179635861
34-35	0.3381582941265746
36-37	0.5195440676990386
38-39	0.47255810363144424
40-41	0.4826160110110372
42-43	0.4315741753497208
44-45	0.4044587112271759
46-47	0.4027578618491634
48-49	0.3929289149627645
50-51	0.3894945075648547
52-53	0.4516082185041946
54-55	0.4527530209701645
56-57	0.4222031494497098
58-59	0.4073370717130431
60-61	0.4219087716727461
62-63	0.40154764209942384
64-65	0.4512157148015763
66-67	0.47296696165500496
68-69	0.41340452478268375
70-71	0.3714066286025298
72-73	0.35712930641979057
74-75	0.3406114422679387
76-77	0.33809287684280487
78-79	0.338747049680502
80-81	0.35431636321769305
82-83	0.34870683113444034
84-85	0.34906662619517376
86-87	0.33519816203599523
88-89	0.3774413730302856
90-91	0.3576199360480634
92-93	0.3471368163239673
94-95	0.3545943866737143
96-97	0.3538747965522475
98	0.3491647521208283
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
98	3057296.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.539323993009994
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.7682524352711	40.030416915231385
2	11.886533833917333	12.727939721759506
3	5.033105785820468	8.084072440744055
4	2.5957679364478805	5.559026422406002
5	1.5288953798817964	4.09280125474538
6	0.973666061990326	3.127765365233893
7	0.6764377527895706	2.5351214005393095
8	0.46937567300917216	2.010404498534015
9	0.35239401964952083	1.6980243832093345
>10	1.6203922014456247	14.835859905691992
>50	0.0727613952511287	2.6109950369475814
>100	0.020633970253732918	1.823023325673977
>500	0.001230037429490657	0.4518337103762371
>1k	5.535168428606818E-4	0.4127156189074809
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	1.3083456753942046E-4	0.0
5	0.0	0.0	0.0	1.3083456753942046E-4	0.0
6	0.0	0.0	0.0	1.3083456753942046E-4	3.2708641884855116E-5
7	0.0	0.0	0.0	2.289604931939858E-4	6.541728376971023E-5
8	0.0	0.0	0.0	3.5979506073340625E-4	9.812592565456534E-5
9	0.0	0.0	0.0	4.579209863879716E-4	1.6354320942427557E-4
10-11	0.0	0.0	0.0	5.396925911001094E-4	1.6354320942427557E-4
12-13	0.0	0.0	0.0	8.013617261789503E-4	1.6354320942427557E-4
14-15	0.0	0.0	0.0	8.994876518335156E-4	2.7802345602126845E-4
16-17	0.0	0.0	0.0	0.001030322219372936	3.5979506073340625E-4
18-19	0.0	0.0	0.0	0.001324699996336632	3.5979506073340625E-4
20-21	0.0	0.0	0.0	0.0013410543172790597	3.5979506073340625E-4
22-23	0.0	0.0	0.0	0.0015700148104730454	4.7427530733039916E-4
24-25	1.6354320942427558E-5	0.0	0.0	0.002322313573824713	7.032358005243849E-4
26-27	3.2708641884855116E-5	0.0	0.0	0.0034016987560249318	7.195901214668125E-4
28-29	3.2708641884855116E-5	0.0	0.0	0.008177160471213779	7.195901214668125E-4
30-31	3.2708641884855116E-5	0.0	0.0	0.028211203625687537	7.359444424092401E-4
32-33	3.2708641884855116E-5	0.0	0.0	0.07173005165348727	8.177160471213779E-4
34-35	3.2708641884855116E-5	0.0	0.0	0.13011497741795364	8.504246890062329E-4
36-37	3.2708641884855116E-5	1.6354320942427558E-5	0.0	0.22644192776885197	8.831333308910881E-4
38-39	3.2708641884855116E-5	3.2708641884855116E-5	0.0	0.3853895730083054	8.831333308910881E-4
40-41	3.2708641884855116E-5	3.2708641884855116E-5	0.0	0.5728918626132373	8.831333308910881E-4
42-43	3.2708641884855116E-5	3.2708641884855116E-5	0.0	0.7726598929249899	8.831333308910881E-4
44-45	3.2708641884855116E-5	3.2708641884855116E-5	0.0	1.01815460459177	8.994876518335156E-4
46-47	3.2708641884855116E-5	3.2708641884855116E-5	0.0	1.3083947383570318	9.812592565456534E-4
48-49	3.2708641884855116E-5	3.2708641884855116E-5	0.0	1.6185544350301706	0.0010793851822002187
50-51	3.2708641884855116E-5	3.2708641884855116E-5	0.0	1.9635488353106796	0.0010793851822002187
52-53	3.2708641884855116E-5	3.2708641884855116E-5	0.0	2.302770160298512	0.001144802465969929
54-55	3.2708641884855116E-5	3.2708641884855116E-5	0.0	2.6812418555481705	0.0012429283916244945
56-57	3.2708641884855116E-5	3.2708641884855116E-5	1.6354320942427558E-5	3.1315253740560287	0.0012756370335093494
58-59	3.2708641884855116E-5	3.2708641884855116E-5	3.2708641884855116E-5	3.608221120885907	0.0013083456753942046
60-61	3.2708641884855116E-5	3.2708641884855116E-5	3.2708641884855116E-5	4.116398935529959	0.0013083456753942046
62-63	3.2708641884855116E-5	3.2708641884855116E-5	3.2708641884855116E-5	4.634896326688682	0.0013410543172790597
64-65	3.2708641884855116E-5	3.2708641884855116E-5	3.2708641884855116E-5	5.186331320225454	0.0013410543172790597
66-67	3.2708641884855116E-5	3.2708641884855116E-5	3.2708641884855116E-5	5.820388343163371	0.0013737629591639147
68-69	3.2708641884855116E-5	3.2708641884855116E-5	3.2708641884855116E-5	6.517507627655288	0.0014064716010487698
70-71	3.2708641884855116E-5	3.2708641884855116E-5	3.2708641884855116E-5	7.246158042924205	0.0015045975267033353
72-73	3.2708641884855116E-5	3.2708641884855116E-5	3.2708641884855116E-5	7.993027171722986	0.0017989753036670312
74-75	3.2708641884855116E-5	4.906296282728268E-5	3.2708641884855116E-5	8.748433256053715	0.0017989753036670312
76-77	3.2708641884855116E-5	6.541728376971023E-5	3.2708641884855116E-5	9.573917605622746	0.0017989753036670312
78-79	3.2708641884855116E-5	6.541728376971023E-5	3.2708641884855116E-5	10.480552095708102	0.0017989753036670312
80-81	3.2708641884855116E-5	6.541728376971023E-5	3.2708641884855116E-5	11.423591304211303	0.0018643925874367415
82-83	3.2708641884855116E-5	6.541728376971023E-5	3.2708641884855116E-5	12.383491817606146	0.0019461641921488793
84-85	3.2708641884855116E-5	6.541728376971023E-5	3.2708641884855116E-5	13.339630837184231	0.0019625185130913068
86	3.2708641884855116E-5	6.541728376971023E-5	3.2708641884855116E-5	14.06438892406885	0.001995227154976162
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2700	0.0	46.201675	1
GTATCAA	4830	0.0	42.21908	1
TCAACGC	5870	0.0	34.68813	4
CAACGCA	6060	0.0	33.524868	5
ATCAACG	6035	0.0	33.511765	3
TATCAAC	6410	0.0	31.482794	2
AACGCAG	6555	0.0	31.128073	6
GTACATG	10395	0.0	28.606205	1
TACATGG	10505	0.0	27.462069	2
ACATGGG	10680	0.0	25.89298	3
ACGCAGA	7905	0.0	24.937527	7
CGCAGAG	8205	0.0	23.85616	8
CATGGGG	7600	0.0	22.205992	4
GCAGAGT	9395	0.0	20.00499	9
TAAGGTG	1450	0.0	19.292913	5
GAGTACA	7320	0.0	18.173515	1
AGAGTAC	7550	0.0	17.517473	10-11
AGTACAT	7505	0.0	16.92808	2
CAGAGTA	8425	0.0	16.813616	10-11
GTAAGGT	1210	0.0	16.67644	4
>>END_MODULE