FastQCFastQC Report
Mon 15 Aug 2016
SRR2920876_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920876_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3057296
Sequences flagged as poor quality0
Sequence length101
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26700.046.4963151
GTATCAA49550.044.4453771
TCAACGC61850.034.692324
ATCAACG64300.033.370453
CAACGCA64200.033.2696385
TATCAAC68500.031.6682932
AACGCAG69900.030.5566646
GTACATG102400.025.4548111
ACGCAGA85050.024.6109147
TACATGG105400.024.139222
TAAGGTG15650.023.353235
CGCAGAG89200.023.1979478
ACATGGG110500.022.4255373
CATGGGG78450.020.0899934
GAGTACA78400.019.9603961
GCAGAGT99600.019.917969
GTAAGGT14650.018.7942494
GGTAAGG13750.018.6434253
AGGTAAG13300.018.5588382
AGAGTAC81200.018.1465610-11
Produced by FastQC (version 0.11.5)