##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920876_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3057296
Sequences flagged as poor quality	0
Sequence length	101
%GC	48
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.20845838937414	31.0	30.0	34.0	26.0	34.0
2	30.22926599190919	31.0	30.0	34.0	26.0	34.0
3	30.363312220995283	31.0	30.0	34.0	26.0	34.0
4	34.003801398359855	35.0	35.0	37.0	30.0	37.0
5	33.51572533375898	35.0	35.0	37.0	28.0	37.0
6	33.46280373244854	35.0	35.0	37.0	28.0	37.0
7	33.27588660044693	35.0	33.0	37.0	27.0	37.0
8	33.20247630585982	35.0	33.0	37.0	27.0	37.0
9	34.531206006876666	38.0	34.0	39.0	27.0	39.0
10-11	34.335927074120406	37.5	34.0	39.0	27.0	39.0
12-13	34.155959383716855	37.0	34.0	39.0	26.0	39.0
14-15	35.05728362579221	38.0	34.0	40.0	26.0	41.0
16-17	34.92749933274371	38.0	34.0	40.0	25.0	41.0
18-19	34.8298516074335	38.0	33.5	40.0	25.0	41.0
20-21	34.7104984273685	38.0	34.0	40.0	24.5	41.0
22-23	34.566729063852506	38.0	33.5	40.0	24.0	41.0
24-25	34.386900875806596	38.0	33.0	40.0	23.5	41.0
26-27	34.19422882180855	38.0	33.0	40.0	22.5	41.0
28-29	34.13770354587845	38.0	33.0	40.0	22.5	41.0
30-31	34.12225018447674	38.0	33.0	40.0	22.0	41.0
32-33	33.97406466367666	38.0	33.0	40.0	21.0	41.0
34-35	33.81141407308942	38.0	33.0	40.0	19.5	41.0
36-37	33.61355410140202	38.0	32.5	40.0	18.5	41.0
38-39	33.43410533360198	38.0	32.0	40.0	18.0	41.0
40-41	33.20451029275543	38.0	32.0	40.0	16.0	41.0
42-43	33.23012721045002	38.0	32.0	40.0	15.5	41.0
44-45	33.08193024162528	38.0	32.0	40.0	15.0	41.0
46-47	32.831678712169186	37.5	31.5	40.0	13.0	41.0
48-49	32.56567780810232	37.0	31.0	40.0	9.0	41.0
50-51	32.29794628979333	37.0	31.0	40.0	8.5	41.0
52-53	32.02788231823153	37.0	31.0	40.0	7.5	41.0
54-55	31.731490670186986	36.0	30.0	39.5	6.5	41.0
56-57	31.397686550468126	36.0	30.0	39.0	2.0	41.0
58-59	31.05165152474605	35.0	29.0	39.0	2.0	40.0
60-61	30.70132267206054	35.0	29.0	39.0	2.0	40.0
62-63	30.364029030882193	35.0	28.5	38.5	2.0	40.0
64-65	29.945902686557012	34.5	28.0	38.0	2.0	40.0
66-67	29.49940077768067	34.0	28.0	37.5	2.0	40.0
68-69	29.048614036717414	34.0	27.0	37.0	2.0	39.0
70-71	28.61593561761766	34.0	26.0	36.0	2.0	39.0
72-73	28.179903254379035	33.5	26.0	36.0	2.0	39.0
74-75	27.73632762414892	33.0	26.0	35.0	2.0	38.0
76-77	26.305229359538625	31.5	24.0	34.0	2.0	36.0
78-79	27.14391622531806	33.0	25.5	35.0	2.0	37.0
80-81	27.18285864371654	33.0	26.0	35.0	2.0	36.0
82-83	26.8551095477834	33.0	25.0	35.0	2.0	36.0
84-85	26.547538085942612	33.0	25.0	35.0	2.0	35.5
86-87	26.020432107326215	32.0	23.5	35.0	2.0	35.0
88-89	25.667307156389175	32.0	21.5	35.0	2.0	35.0
90-91	25.30917287694747	32.0	20.0	34.5	2.0	35.0
92-93	25.005683944243543	32.0	19.0	34.0	2.0	35.0
94-95	24.629076641581317	32.0	16.5	34.0	2.0	35.0
96-97	24.105929062805828	31.0	4.5	34.0	2.0	35.0
98-99	23.495602650185	31.0	2.0	34.0	2.0	35.0
100-101	21.299005395617566	28.0	2.0	33.0	2.0	34.5
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	75519.0
3	33804.0
4	18204.0
5	13004.0
6	11079.0
7	11181.0
8	12336.0
9	13927.0
10	15856.0
11	17984.0
12	19619.0
13	21402.0
14	23075.0
15	24677.0
16	25906.0
17	26906.0
18	26283.0
19	25301.0
20	25811.0
21	26941.0
22	28921.0
23	32453.0
24	38149.0
25	44741.0
26	52897.0
27	62781.0
28	74634.0
29	87489.0
30	102969.0
31	122229.0
32	146035.0
33	178748.0
34	225757.0
35	293460.0
36	391158.0
37	449009.0
38	242074.0
39	14977.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.45846104739018	19.0307572262424	13.609727690089803	24.90105403627762
2	16.192936503367683	20.359886644930683	37.69831249574788	25.74886435595376
3	19.328560170862946	24.1026603941899	29.108134799586388	27.460644635360765
4	14.051520103112175	16.159791158588945	35.34467501836031	34.44401371993857
5	14.71977191448494	36.91169307896146	33.74153077803499	14.627004228518606
6	34.175718533915116	34.815193908710555	16.63804907948944	14.371038477884893
7	29.686183670073596	31.343342736118878	20.480636070273267	18.48983752353426
8	27.257856242961008	34.23827593468143	19.658512926199766	18.845354896157787
9	26.96637015787562	15.507768984527539	19.030793917928523	38.49506693966831
10-11	26.0519322447492	25.129013608911656	27.159012189389475	21.660041956949673
12-13	26.51321431623785	22.377807401615076	27.221992457382445	23.886985824764633
14-15	23.6970383245596	23.956992113463436	24.462737064364145	27.88323249761282
16-17	23.97297700227161	27.06579188750389	25.20392685095375	23.757304259270747
18-19	24.114493059613185	26.137671872979713	26.525518462689178	23.222316604717925
20-21	24.659301359195346	25.37983194927067	26.268642309781363	23.69222438175262
22-23	24.40742915215332	25.222211510494862	26.221833908465797	24.148525428886014
24-25	24.235285262066704	25.429351503444124	26.18497218601782	24.150391048471356
26-27	23.963613197032014	25.694550701992597	26.109905661844014	24.23193043913138
28-29	24.04097728763025	25.69255843661203	26.172936901940098	24.093527373817622
30-31	24.289719957919996	25.521595611535	25.934592778119576	24.254091652425434
32-33	24.04555907332964	25.587168625926687	25.806889089670847	24.560383211072825
34-35	24.384934068269068	25.625363036322526	25.930072272751737	24.059630622656673
36-37	24.563506233966056	25.35386114284116	25.828555014449872	24.254077608742914
38-39	24.33077963783657	25.419936492979385	25.881024946382208	24.368258922801832
40-41	24.452027758615923	25.314499334138052	26.041115769027613	24.192357138218416
42-43	24.383286546510977	25.21135033099162	25.865826090412654	24.539537032084752
44-45	24.178732383998167	25.2599929658681	26.001537693949828	24.559736956183905
46-47	24.404408423040564	25.26604611869591	25.828926906149892	24.50061855211363
48-49	24.17131219451879	25.335092974888962	25.740040601073844	24.753554229518407
50-51	24.11046311448931	25.471959356953995	25.923517861071378	24.494059667485317
52-53	24.351921619720418	25.204822682183135	25.93062598142583	24.512629716670613
54-55	23.91154795816492	25.264658699699964	26.017662668353747	24.80613067378137
56-57	23.958136049334982	25.367377559621886	25.91463723786374	24.759849153179392
58-59	23.802759129607654	25.194966237035505	26.005774572516778	24.996500060840063
60-61	23.76719435470798	25.242689224802895	26.07653087847733	24.913585542011795
62-63	23.696442088335758	25.37021371807428	26.02537832619893	24.90796586739103
64-65	23.52164393518244	25.245710523049027	25.982780759630806	25.24986478213773
66-67	23.462926154653108	25.277790639023586	25.942570710902224	25.31671249542108
68-69	23.67105341125346	25.082867339252097	25.920134381769543	25.325944867724903
70-71	23.40216501052865	24.921339782857725	26.01128781272497	25.665207393888657
72-73	23.419348969684116	24.95152089886111	25.864246736536785	25.76488339491799
74-75	23.388866901122814	25.069801609242585	25.84659719405551	25.694734295579085
76-77	23.32594581106502	24.807827691371422	26.117727528636074	25.748498968927485
78-79	23.229426703303393	24.894561383867924	25.999391788423555	25.876620124405125
80-81	23.238676484515658	24.93310435850928	25.963572851271433	25.864646305703637
82-83	23.11679415682467	24.864817034394864	26.01924521290377	25.9991435958767
84-85	22.949516520162085	24.791005662270386	26.042632718560398	26.21684509900713
86-87	23.006299951135205	24.881019142537625	25.96976941719713	26.142911489130043
88-89	22.812048202684966	24.736913563485107	25.92567175600785	26.525366477822075
90-91	22.6843272537951	24.758011421168927	25.93082526759397	26.626836057442006
92-93	22.726448378926065	24.60433480109116	26.02047573866722	26.648741081315556
94-95	22.387562476193995	24.459627225580252	26.165318702389012	26.987491595836737
96-97	22.521298396711977	24.283001679666224	26.215484658121685	26.980215265500114
98-99	22.362958080718045	24.32446842438387	26.12452304979908	27.188050445099005
100-101	22.450811036069997	24.080700942940442	26.21040456452912	27.258083456460437
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	91.0
1	62.0
2	71.0
3	117.5
4	107.0
5	114.0
6	182.0
7	360.5
8	554.5
9	609.5
10	513.0
11	422.5
12	415.5
13	444.0
14	477.0
15	490.5
16	545.5
17	601.0
18	678.5
19	793.5
20	880.0
21	1005.5
22	1183.0
23	1429.5
24	1782.5
25	2240.5
26	2825.0
27	3683.0
28	4952.5
29	6838.5
30	9823.0
31	13958.0
32	19553.5
33	24684.0
34	29252.5
35	35508.0
36	43904.0
37	52271.5
38	59410.0
39	66747.5
40	73884.5
41	80579.0
42	87601.5
43	96225.0
44	105356.5
45	114747.0
46	123909.0
47	129874.0
48	134843.0
49	139938.5
50	142173.0
51	142241.0
52	140834.0
53	140655.0
54	139443.0
55	132844.0
56	122455.5
57	110502.0
58	100371.0
59	88210.5
60	72833.0
61	59929.0
62	49146.5
63	37679.0
64	27316.5
65	19960.5
66	14745.5
67	10992.0
68	8036.0
69	5867.0
70	4344.0
71	3065.5
72	2126.5
73	1609.0
74	1209.5
75	850.0
76	587.5
77	353.0
78	193.0
79	102.0
80	58.0
81	26.0
82	14.5
83	10.0
84	5.5
85	3.5
86	3.0
87	2.0
88	1.0
89	2.0
90	1.0
91	0.0
92	0.5
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.19242494020860265
2	0.0
3	0.011219064166505303
4	0.014097424652372554
5	0.006443602451316458
6	0.10564891328808201
7	0.140647160104877
8	0.12327887126401893
9	0.14139945886822866
10-11	0.11289387746557743
12-13	0.11281210586086528
14-15	0.11395690832683521
16-17	0.06451779611787671
18-19	0.07689801707129437
20-21	0.035194498668104104
22-23	0.08152628989800137
24-25	0.05653688749797206
26-27	0.06402716648960388
28-29	0.03807285915397135
30-31	0.024122623390080648
32-33	0.02984663571993029
34-35	0.0200013345125889
36-37	0.02271615178903188
38-39	0.030778832013648665
40-41	0.023926371538771515
42-43	0.011300835771217441
44-45	0.02521836289322329
46-47	0.03156383941888519
48-49	0.0076538222010560965
50-51	0.014342739466508967
52-53	0.00655808269791345
54-55	0.011120938240850739
56-57	0.005069839492152543
58-59	0.0032708641884855115
60-61	0.021604057964946802
62-63	0.009093002443989723
64-65	0.0062964135628346095
66-67	0.03835088260999262
68-69	0.08880396271738163
70-71	0.02081905055971028
72-73	0.043829580125705855
74-75	0.08396308371842308
76-77	0.24724462400761982
78-79	0.07974366891527676
80-81	0.015781919709442593
82-83	0.010728434538232478
84-85	0.012919913544517771
86-87	0.2638279054432413
88-89	0.6241953674096326
90-91	0.2660357387704691
92-93	0.3536621903799959
94-95	0.001030322219372936
96-97	0.004611918505764571
98-99	0.0036306592492189177
100-101	0.005135256775922253
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3057296.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.30757546264096
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.13034715264972	36.28702882528415
2	12.846542410014747	12.925568034516688
3	5.533724321615142	8.351647616973162
4	2.9152634053886834	5.866393350402675
5	1.7752873480921658	4.465520111600919
6	1.1180543229546738	3.3747961334024525
7	0.7631434549149211	2.6874327862867053
8	0.5259254416953683	2.1166427076650023
9	0.41317374977025806	1.8707192636174634
>10	1.867740107740599	16.152906004096764
>50	0.08318485485818991	2.8148321711666098
>100	0.025459532369480852	2.067001363779187
>500	0.001501201592039082	0.5410973678204897
>1k	6.526963440512683E-4	0.47841426338773974
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	3.2708641884855116E-5	0.0
3	0.0	0.0	0.0	3.2708641884855116E-5	0.0
4	0.0	0.0	0.0	3.2708641884855116E-5	1.3083456753942046E-4
5	0.0	0.0	0.0	3.2708641884855116E-5	1.3083456753942046E-4
6	0.0	0.0	0.0	3.2708641884855116E-5	2.9437777696369604E-4
7	0.0	0.0	0.0	1.6354320942427557E-4	2.9437777696369604E-4
8	0.0	0.0	0.0	1.6354320942427557E-4	2.9437777696369604E-4
9	0.0	0.0	0.0	2.616691350788409E-4	3.2708641884855114E-4
10-11	0.0	0.0	0.0	3.107320979061236E-4	3.2708641884855114E-4
12-13	0.0	0.0	0.0	3.9250370261826136E-4	3.2708641884855114E-4
14-15	0.0	0.0	0.0	4.088580235606889E-4	3.7614938167583383E-4
16-17	0.0	0.0	0.0	5.724012329849645E-4	4.579209863879716E-4
18-19	0.0	0.0	0.0	8.340703680638055E-4	5.233382701576817E-4
20-21	0.0	0.0	0.0	8.831333308910881E-4	5.724012329849645E-4
22-23	0.0	0.0	0.0	0.0010139678984305085	7.195901214668125E-4
24-25	0.0	0.0	0.0	0.0017826209827246037	8.504246890062329E-4
26-27	0.0	0.0	0.0	0.0027965888811551124	8.831333308910881E-4
28-29	0.0	0.0	0.0	0.007408507386919683	8.994876518335156E-4
30-31	3.2708641884855116E-5	0.0	0.0	0.027409841899508583	9.158419727759432E-4
32-33	3.2708641884855116E-5	0.0	0.0	0.07120671338332958	9.812592565456534E-4
34-35	3.2708641884855116E-5	0.0	0.0	0.12947715890119896	0.0010139678984305085
36-37	3.2708641884855116E-5	0.0	0.0	0.22608213270811855	0.0010466765403153637
38-39	3.2708641884855116E-5	0.0	0.0	0.3861582260925995	0.0011120938240850738
40-41	3.2708641884855116E-5	3.2708641884855116E-5	0.0	0.5752305305080045	0.001144802465969929
42-43	3.2708641884855116E-5	3.2708641884855116E-5	0.0	0.7758980484715906	0.0011775111078547841
44-45	3.2708641884855116E-5	3.2708641884855116E-5	0.0	1.0252850885226683	0.0012102197497396393
46-47	3.2708641884855116E-5	3.2708641884855116E-5	0.0	1.3163920012978787	0.0012265740706820668
48-49	3.2708641884855116E-5	3.2708641884855116E-5	0.0	1.6274838942647358	0.0012756370335093494
50-51	3.2708641884855116E-5	3.2708641884855116E-5	0.0	1.969027532826393	0.0012756370335093494
52-53	3.2708641884855116E-5	3.2708641884855116E-5	0.0	2.304209340541446	0.0014882432057609076
54-55	3.2708641884855116E-5	3.2708641884855116E-5	0.0	2.678428912346073	0.0017172036989548936
56-57	3.2708641884855116E-5	3.2708641884855116E-5	0.0	3.123168316054448	0.0017662666617821762
58-59	3.2708641884855116E-5	3.2708641884855116E-5	0.0	3.5954320419089285	0.0017989753036670312
60-61	3.2708641884855116E-5	3.2708641884855116E-5	0.0	4.099684819526797	0.0018971012293215966
62-63	3.2708641884855116E-5	6.541728376971023E-5	0.0	4.612523615639441	0.0018971012293215966
64-65	3.2708641884855116E-5	6.541728376971023E-5	0.0	5.156975314133797	0.0018971012293215966
66-67	3.2708641884855116E-5	6.541728376971023E-5	0.0	5.7845069630156845	0.0018971012293215966
68-69	3.2708641884855116E-5	6.541728376971023E-5	0.0	6.474577535181416	0.0019298098712064518
70-71	3.2708641884855116E-5	6.541728376971023E-5	0.0	7.196048403556607	0.0020933530806307274
72-73	3.2708641884855116E-5	6.541728376971023E-5	0.0	7.939384999031824	0.002289604931939858
74-75	3.2708641884855116E-5	6.541728376971023E-5	0.0	8.693957012994488	0.002289604931939858
76-77	3.2708641884855116E-5	6.541728376971023E-5	0.0	9.519882929228967	0.002305959252882285
78-79	3.2708641884855116E-5	6.541728376971023E-5	0.0	10.423377389693375	0.002322313573824713
80-81	3.2708641884855116E-5	6.541728376971023E-5	0.0	11.362622395737933	0.002371376536651996
82-83	3.2708641884855116E-5	6.541728376971023E-5	0.0	12.317207754826487	0.0024858567832489885
84-85	3.2708641884855116E-5	6.541728376971023E-5	0.0	13.271760405273156	0.0024858567832489885
86-87	3.2708641884855116E-5	8.177160471213779E-5	0.0	14.249879632197864	0.0024858567832489885
88-89	3.2708641884855116E-5	9.812592565456534E-5	0.0	15.299107446580248	0.0024858567832489885
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2670	0.0	46.496315	1
GTATCAA	4955	0.0	44.445377	1
TCAACGC	6185	0.0	34.69232	4
ATCAACG	6430	0.0	33.37045	3
CAACGCA	6420	0.0	33.269638	5
TATCAAC	6850	0.0	31.668293	2
AACGCAG	6990	0.0	30.556664	6
GTACATG	10240	0.0	25.454811	1
ACGCAGA	8505	0.0	24.610914	7
TACATGG	10540	0.0	24.13922	2
TAAGGTG	1565	0.0	23.35323	5
CGCAGAG	8920	0.0	23.197947	8
ACATGGG	11050	0.0	22.425537	3
CATGGGG	7845	0.0	20.089993	4
GAGTACA	7840	0.0	19.960396	1
GCAGAGT	9960	0.0	19.91796	9
GTAAGGT	1465	0.0	18.794249	4
GGTAAGG	1375	0.0	18.643425	3
AGGTAAG	1330	0.0	18.558838	2
AGAGTAC	8120	0.0	18.14656	10-11
>>END_MODULE