FastQCFastQC Report
Mon 15 Aug 2016
SRR2920875_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920875_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4422198
Sequences flagged as poor quality0
Sequence length98
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA41500.047.0240971
GTATCAA75150.043.300521
TCAACGC97550.033.094694
ATCAACG100150.032.1439363
CAACGCA101500.031.7615855
TATCAAC103000.031.3453392
AACGCAG107150.030.383646
GTACATG138900.026.2105981
ACGCAGA126250.025.4186137
CGCAGAG128150.024.7904918
TACATGG144150.024.2107092
ACATGGG148250.022.7355583
GCAGAGT142400.020.9568869
CATGGGG111500.019.6180764
GAGTACA106500.018.799331
AGAGTAC122450.018.75724610-11
TAAGGCG13150.018.48265
GTGGTAT23000.018.0101531
GTGTAAG17400.017.9871521
TAAGGTG18950.017.6655255
Produced by FastQC (version 0.11.5)