##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920875_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4422198
Sequences flagged as poor quality	0
Sequence length	98
%GC	48
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.581093610010225	31.0	29.0	33.0	22.0	34.0
2	28.613643260659067	31.0	28.0	34.0	18.0	34.0
3	28.68006023249072	31.0	28.0	34.0	20.0	34.0
4	32.1622555118518	35.0	32.0	37.0	22.0	37.0
5	32.12311185523579	35.0	32.0	37.0	22.0	37.0
6	32.25062242803239	35.0	32.0	37.0	23.0	37.0
7	32.08572999218941	35.0	32.0	37.0	22.0	37.0
8	32.03451858103142	35.0	32.0	37.0	22.0	37.0
9	33.29424462676705	37.0	33.0	39.0	22.0	39.0
10-11	33.10031436855609	37.0	32.0	39.0	19.0	39.0
12-13	32.92569034674612	37.0	32.0	39.0	18.0	39.0
14-15	33.675079903703995	38.0	32.0	40.0	17.0	41.0
16-17	33.56408261231179	38.0	32.0	40.0	17.0	41.0
18-19	33.45741371146204	38.0	32.0	40.0	17.5	41.0
20-21	33.365464074652465	38.0	32.0	40.0	17.5	41.0
22-23	33.146014945508995	38.0	32.0	40.0	16.0	41.0
24-25	32.951420650997534	38.0	32.0	40.0	14.0	41.0
26-27	32.981174972264924	38.0	32.0	40.0	10.5	41.0
28-29	32.819473031284446	38.0	31.5	40.0	10.0	41.0
30-31	32.57121051567569	37.5	31.0	40.0	9.5	41.0
32-33	32.32114855553731	37.0	30.5	40.0	8.5	41.0
34-35	31.99052269934544	37.0	30.0	40.0	7.5	41.0
36-37	31.681518557061445	37.0	30.0	40.0	2.0	41.0
38-39	31.48063519543901	37.0	30.0	40.0	2.0	41.0
40-41	31.280075767751693	37.0	30.0	40.0	2.0	41.0
42-43	31.044949253742143	36.0	29.5	40.0	2.0	41.0
44-45	30.75927321662214	36.0	29.0	39.0	2.0	40.5
46-47	30.503123559822512	36.0	28.0	39.0	2.0	40.0
48-49	30.199361946253877	35.5	28.0	39.0	2.0	40.0
50-51	29.052538805363305	34.0	25.5	38.0	2.0	39.5
52-53	29.499908529649737	34.5	27.5	38.5	2.0	39.5
54-55	30.150546176358453	35.0	28.0	39.0	2.0	40.0
56-57	30.1022671305084	35.0	28.0	39.0	2.0	40.5
58-59	29.856979379937307	35.0	28.0	39.0	2.0	40.0
60-61	29.531824671803477	35.0	27.5	39.0	2.0	40.0
62-63	29.143858664853994	34.5	26.5	38.5	2.0	40.0
64-65	28.730671715739547	34.0	26.0	38.0	2.0	40.0
66-67	28.35036502209987	34.0	26.0	37.5	2.0	40.0
68-69	27.912343251025845	34.0	25.0	37.0	2.0	39.5
70-71	27.59183634473174	34.0	25.0	36.5	2.0	39.0
72-73	27.1377177141322	33.0	24.0	36.0	2.0	39.0
74-75	26.706131995898872	33.0	23.0	35.5	2.0	38.5
76-77	26.296991790055536	33.0	22.0	35.0	2.0	37.5
78-79	25.837119572664996	32.0	20.0	35.0	2.0	37.0
80-81	25.40356639390638	32.0	19.0	35.0	2.0	36.0
82-83	24.93930167758205	32.0	17.5	35.0	2.0	36.0
84-85	24.541584750388836	31.5	11.5	34.5	2.0	35.5
86-87	24.089729926158892	31.0	6.0	34.0	2.0	35.0
88-89	23.559235249077496	31.0	2.0	34.0	2.0	35.0
90-91	23.05865352478564	31.0	2.0	34.0	2.0	35.0
92-93	22.524155521756377	30.0	2.0	34.0	2.0	35.0
94-95	21.88928684332995	30.0	2.0	34.0	2.0	35.0
96-97	21.250193908097287	29.0	2.0	34.0	2.0	35.0
98	20.854036160298566	29.0	2.0	34.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	223666.0
3	38355.0
4	28349.0
5	23150.0
6	21571.0
7	22061.0
8	25137.0
9	29192.0
10	31766.0
11	33821.0
12	35340.0
13	35213.0
14	33333.0
15	34908.0
16	38082.0
17	40294.0
18	41776.0
19	40822.0
20	42021.0
21	44899.0
22	49001.0
23	54391.0
24	62421.0
25	71737.0
26	82692.0
27	95871.0
28	111179.0
29	129404.0
30	150475.0
31	176782.0
32	209811.0
33	253922.0
34	314680.0
35	396046.0
36	499731.0
37	542274.0
38	324777.0
39	33248.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.73508682360211	18.282342594835374	13.302297960014812	24.680272621547708
2	16.025245198160285	19.817114132669612	39.00685301772086	25.150787651449246
3	18.9775106719929	23.902730953596905	29.42018633975862	27.699572034651577
4	13.477983075821811	15.823416793226711	36.43988388516528	34.258716245786204
5	14.464006904876772	36.79133579102434	34.1052464442531	14.639410859845794
6	33.23955028468535	34.97763162329116	17.370861330087756	14.411956761935738
7	28.937101056401183	31.675457382199472	21.186623336177753	18.200818225221592
8	26.685399865211433	34.008354177314814	20.319557459280734	18.98668849819302
9	27.013828869715915	15.45227056771316	19.676957024538478	37.856943538032446
10-11	25.677694666769785	25.10163497880466	27.755575394860205	21.465094959565356
12-13	26.226857675588246	22.649256579241207	27.53912718518891	23.584758559981637
14-15	23.551536242843657	24.178758280824557	25.00370587004161	27.265999606290176
16-17	23.574463369722107	27.03285731488962	25.83019037534014	23.562488940048134
18-19	23.57475126796417	26.392498434308422	27.151054371828252	22.881695925899155
20-21	24.34550886007197	25.627198044673772	26.855557064294928	23.17173603095933
22-23	24.12121519413689	25.41222428405414	26.695559979012472	23.771000542796493
24-25	23.85608070374564	25.56586708736449	26.65824003852173	23.91981217036814
26-27	23.652042304940586	25.755623578766425	26.648523455919566	23.94381066037342
28-29	23.76332362929683	25.963413071001167	26.49511945377433	23.778143845927673
30-31	24.043619412077334	25.74287868843112	26.273822266597612	23.93967963289393
32-33	23.7384169586164	25.849728376202325	26.239355317989887	24.172499347191383
34-35	24.138344165403065	25.799431706338872	26.30798022034001	23.754243907918045
36-37	24.274127103865546	25.511518231597602	26.270065168500757	23.944289496036095
38-39	24.097783880301566	25.61692906546975	26.252214364452836	24.033072689775846
40-41	24.172174896288944	25.490616467243303	26.419800722068914	23.91740791439884
42-43	24.084366753651093	25.436329342815426	26.19947486829974	24.279829035233746
44-45	23.86027928747509	25.50908787261481	26.39844406093194	24.232188778978156
46-47	24.031120401905444	25.453197749698266	26.24182285657261	24.27385899182368
48-49	23.878759519484422	25.571203121158064	26.116563465840425	24.433473893517093
50-51	23.795258234396616	25.72123087799547	26.226451483349813	24.2570594042581
52-53	23.895053917815936	25.50765889683717	26.311065021682982	24.28622216366391
54-55	23.551286967489148	25.439734337488435	26.577848220889894	24.431130474132527
56-57	23.52859686258274	25.510180328421722	26.479902499766368	24.481320309229172
58-59	23.543267724791313	25.335499129355902	26.46194921414961	24.659283931703175
60-61	23.43770776332964	25.251473469598633	26.725675473892007	24.585143293179716
62-63	23.433070233238894	25.240749982857093	26.717128874240093	24.60905090966392
64-65	23.271976132867582	25.155058896597186	26.6597372988914	24.91322767164383
66-67	23.276029347275333	25.19622411720733	26.616213256261517	24.91153327925582
68-69	23.35483038809036	25.051829441030023	26.614723115141576	24.978617055738038
70-71	23.135872636335293	24.90693223613568	26.769206813305967	25.18798831422306
72-73	23.159266861424662	25.03934271899835	26.704559897161968	25.096830522415026
74-75	23.17599945903433	25.070355035950165	26.74372931384949	25.009916191166013
76-77	23.19658562038172	24.79356461418216	26.861622799229906	25.148226966206217
78-79	23.15526617041578	24.825875388156916	26.86751553478578	25.151342906641528
80-81	23.2327642894808	24.834285925670905	26.78985668165162	25.14309310319668
82-83	23.16944841141334	24.796999995688214	26.897406960175214	25.13614463272323
84-85	23.13597102114626	24.796622552903667	26.85922360336896	25.20818282258111
86-87	23.15588891178091	24.72402887661998	26.90561322428664	25.21446898731248
88-89	23.105256801752844	24.486855044552016	27.03481667524746	25.373071478447685
90-91	23.099440322155473	24.48818941420098	27.070723484693314	25.341646778950235
92-93	23.333870023236518	24.291617550463318	27.046550076742115	25.327962349558046
94-95	23.223907702059474	24.14331948458745	27.203679371934996	25.429093441418082
96-97	23.371201091891404	23.924125439512842	27.2013882852707	25.503285183325055
98	23.37313503287861	23.777264194446765	27.127828087158278	25.72177268551635
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	119.0
1	108.0
2	152.0
3	229.5
4	251.5
5	286.0
6	446.5
7	862.0
8	1294.5
9	1492.5
10	1473.5
11	1508.5
12	1722.0
13	1914.5
14	2184.0
15	2419.5
16	2511.0
17	2617.0
18	2753.0
19	2792.5
20	2810.5
21	2925.5
22	3154.0
23	3568.0
24	4128.5
25	5011.5
26	6442.5
27	8542.0
28	11844.0
29	16698.5
30	23653.5
31	33166.5
32	42643.5
33	51330.0
34	61457.5
35	73323.0
36	86107.5
37	97962.5
38	109204.0
39	119579.5
40	128272.5
41	137056.5
42	146992.5
43	157217.5
44	166794.5
45	176506.5
46	185648.5
47	192894.0
48	197526.5
49	198259.0
50	197486.0
51	196973.5
52	195303.0
53	193371.0
54	187858.5
55	177475.0
56	163342.0
57	146374.0
58	131974.5
59	115955.0
60	97557.0
61	80729.0
62	65583.5
63	50715.5
64	36818.0
65	27223.0
66	20179.0
67	15120.5
68	11148.0
69	8123.5
70	6147.0
71	4574.5
72	3471.5
73	2590.0
74	1997.0
75	1452.5
76	905.0
77	546.5
78	329.0
79	202.0
80	105.0
81	59.0
82	39.0
83	28.5
84	19.5
85	16.5
86	15.5
87	15.0
88	15.5
89	9.5
90	8.0
91	7.0
92	5.5
93	7.5
94	5.5
95	1.5
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.38706995932791793
2	0.024422244322845788
3	0.018361909620509983
4	0.016123203890915785
5	0.02204785945812467
6	0.022635802377007994
7	0.015309129080154258
8	0.010356840648021639
9	0.0
10-11	0.0
12-13	0.005438471999670752
14-15	0.003606803675457318
16-17	0.006229933621244459
18-19	0.03680296540317733
20-21	0.07910093577899498
22-23	0.09805078831838829
24-25	0.06499030572579519
26-27	0.0742051803198319
28-29	0.057878457726225734
30-31	0.05271134399680883
32-33	0.02074760107982501
34-35	0.3424880568441304
36-37	0.5215392888332906
38-39	0.47724005121435087
40-41	0.486409699430012
42-43	0.4364910842978989
44-45	0.41087034094809866
46-47	0.40878992754281923
48-49	0.3997785716514729
50-51	0.3954707591111931
52-53	0.4551582719724444
54-55	0.4572839117560996
56-57	0.42798852516327857
58-59	0.4132447258128198
60-61	0.42740058224439526
62-63	0.4080323857050272
64-65	0.4559045072156425
66-67	0.4773191973765082
68-69	0.4199608430016023
70-71	0.37699578354474406
72-73	0.3628512337077625
74-75	0.3451677197628871
76-77	0.34348303716839457
78-79	0.3426576557630391
80-81	0.35928965641068084
82-83	0.35437128776232996
84-85	0.35445043392448733
86-87	0.3399666862496885
88-89	0.3846503480848212
90-91	0.36186755997809233
92-93	0.35178207760032454
94-95	0.3596062410593103
96-97	0.35952709489715295
98	0.35471048560014723
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
98	4422198.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.08699199347324
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.54749394752402	38.829547203781786
2	12.020749164614774	12.5224933098568
3	5.046270032665383	7.885350803650374
4	2.59377537440598	5.404078286382093
5	1.5091009311073185	3.9302264057964957
6	0.962990120619115	3.0095555221489003
7	0.6396276600275794	2.3321396564662384
8	0.4746575147201085	1.9778785735094557
9	0.35143811598973385	1.6474818900382728
>10	1.7338001950167814	15.89123540764368
>50	0.08974839842097261	3.19069031638846
>100	0.028558249176236165	2.4379641377338586
>500	0.0012226412000604495	0.4418283650948697
>1k	5.676545118938389E-4	0.49953012150844595
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.5226378375640346E-5
2	2.2613189187820173E-5	2.2613189187820173E-5	0.0	0.0	4.5226378375640346E-5
3	2.2613189187820173E-5	2.2613189187820173E-5	0.0	0.0	4.5226378375640346E-5
4	2.2613189187820173E-5	2.2613189187820173E-5	0.0	0.0	6.783956756346053E-5
5	2.2613189187820173E-5	2.2613189187820173E-5	0.0	0.0	6.783956756346053E-5
6	2.2613189187820173E-5	2.2613189187820173E-5	0.0	0.0	6.783956756346053E-5
7	2.2613189187820173E-5	2.2613189187820173E-5	0.0	0.0	9.045275675128069E-5
8	2.2613189187820173E-5	2.2613189187820173E-5	0.0	0.0	9.045275675128069E-5
9	2.2613189187820173E-5	2.2613189187820173E-5	0.0	0.0	9.045275675128069E-5
10-11	2.2613189187820173E-5	6.783956756346053E-5	0.0	0.0	9.045275675128069E-5
12-13	2.2613189187820173E-5	1.2437254053301096E-4	0.0	0.0	2.0351870269038158E-4
14-15	2.2613189187820173E-5	2.0351870269038155E-4	0.0	0.0	2.60051675659932E-4
16-17	2.2613189187820173E-5	2.2613189187820174E-4	0.0	0.0	2.9397145944166225E-4
18-19	2.2613189187820173E-5	2.2613189187820174E-4	0.0	1.1306594593910087E-5	2.9397145944166225E-4
20-21	2.2613189187820173E-5	2.713582702538421E-4	0.0	6.783956756346052E-5	3.8442421619294296E-4
22-23	2.2613189187820173E-5	2.713582702538421E-4	0.0	2.0351870269038155E-4	3.957308107868531E-4
24-25	2.2613189187820173E-5	3.2789124322339257E-4	0.0	0.0010402067026397282	4.2965059456858334E-4
26-27	2.2613189187820173E-5	3.8442421619294296E-4	0.0	0.0020125738377159957	4.974901621320439E-4
28-29	2.2613189187820173E-5	4.2965059456858334E-4	0.0	0.0066143578374374	4.974901621320439E-4
30-31	2.2613189187820173E-5	4.409571891624934E-4	0.0	0.028571764538810787	5.20103351319864E-4
32-33	2.2613189187820173E-5	4.522637837564035E-4	0.0	0.0812718019410257	5.540231351015943E-4
34-35	2.2613189187820173E-5	4.522637837564035E-4	0.0	0.15104479718004488	5.879429188833245E-4
36-37	2.2613189187820173E-5	4.7487697294422367E-4	0.0	0.27285074074023824	5.879429188833245E-4
38-39	2.2613189187820173E-5	5.540231351015943E-4	0.0	0.46904277013376605	6.105561080711446E-4
40-41	2.2613189187820173E-5	5.653297296955044E-4	0.0	0.6937161113093534	6.331692972589649E-4
42-43	2.2613189187820173E-5	5.992495134772346E-4	0.0	0.9335855156191559	6.670890810406951E-4
44-45	2.2613189187820173E-5	6.331692972589649E-4	0.0	1.2286311015472395	7.236220540102456E-4
46-47	2.2613189187820173E-5	6.331692972589649E-4	0.0	1.5797573966611176	7.575418377919758E-4
48-49	2.2613189187820173E-5	6.897022702285153E-4	0.0	1.9515521466926629	8.593011891371667E-4
50-51	2.2613189187820173E-5	7.236220540102455E-4	0.0	2.352687509695405	8.819143783249868E-4
52-53	2.2613189187820173E-5	7.462352431980658E-4	0.0	2.758130685238427	9.384473512945373E-4
54-55	2.2613189187820173E-5	7.462352431980658E-4	0.0	3.213526395697343	9.949803242640877E-4
56-57	2.2613189187820173E-5	7.575418377919758E-4	0.0	3.739452643233071	0.001017593513451908
58-59	2.2613189187820173E-5	8.140748107615262E-4	0.0	4.2974783128209095	0.0010628198918275482
60-61	2.2613189187820173E-5	8.593011891371667E-4	0.0	4.897315769217027	0.0010967396756092784
62-63	2.2613189187820173E-5	8.593011891371667E-4	0.0	5.503145720747918	0.001153272648578829
64-65	2.2613189187820173E-5	9.271407567006272E-4	0.0	6.153071391195057	0.0011871924323605591
66-67	2.2613189187820173E-5	9.497539458884473E-4	0.0	6.890589249961218	0.0012098056215483794
68-69	2.2613189187820173E-5	9.723671350762675E-4	0.0	7.69895423045282	0.0012211122161422895
70-71	2.2613189187820173E-5	9.723671350762675E-4	0.0	8.552930013536255	0.0012324188107361994
72-73	2.2613189187820173E-5	9.723671350762675E-4	0.0	9.413124423646341	0.0013002583782996601
74-75	2.2613189187820173E-5	9.723671350762675E-4	0.0	10.297017456025262	0.0013680979458631204
76-77	2.2613189187820173E-5	9.723671350762675E-4	0.0	11.254007622453813	0.0014020177296448508
78-79	4.5226378375640346E-5	9.723671350762675E-4	0.0	12.299087467363513	0.0014811638918022214
80-81	4.5226378375640346E-5	9.836737296701776E-4	0.0	13.377476539946876	0.0015263902701778617
82-83	4.5226378375640346E-5	0.0010062869188579978	0.0	14.478037844528897	0.0016281496215230526
84-85	4.5226378375640346E-5	0.001017593513451908	0.0	15.576778787381297	0.0016281496215230526
86	4.5226378375640346E-5	0.001017593513451908	0.0	16.420409036411304	0.0016281496215230526
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	4150	0.0	47.024097	1
GTATCAA	7515	0.0	43.30052	1
TCAACGC	9755	0.0	33.09469	4
ATCAACG	10015	0.0	32.143936	3
CAACGCA	10150	0.0	31.761585	5
TATCAAC	10300	0.0	31.345339	2
AACGCAG	10715	0.0	30.38364	6
GTACATG	13890	0.0	26.210598	1
ACGCAGA	12625	0.0	25.418613	7
CGCAGAG	12815	0.0	24.790491	8
TACATGG	14415	0.0	24.210709	2
ACATGGG	14825	0.0	22.735558	3
GCAGAGT	14240	0.0	20.956886	9
CATGGGG	11150	0.0	19.618076	4
GAGTACA	10650	0.0	18.79933	1
AGAGTAC	12245	0.0	18.757246	10-11
TAAGGCG	1315	0.0	18.4826	5
GTGGTAT	2300	0.0	18.010153	1
GTGTAAG	1740	0.0	17.987152	1
TAAGGTG	1895	0.0	17.665525	5
>>END_MODULE