FastQCFastQC Report
Mon 15 Aug 2016
SRR2920875_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920875_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4422198
Sequences flagged as poor quality0
Sequence length101
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA43250.050.2553021
GTATCAA74850.043.208471
TCAACGC103800.030.1822454
TATCAAC104950.030.0303972
ATCAACG105550.029.72683
CAACGCA108050.028.8596765
AACGCAG111750.028.1165056
GTACATG145150.026.2134821
TACATGG150950.024.3064672
ACGCAGA131250.023.4690917
ACATGGG153500.023.0384273
CGCAGAG134400.022.7770028
CATGGGG113300.020.3615724
GAGTACA113300.019.5197751
GCAGAGT148900.019.2842049
AGAGTAC127200.018.52448510-11
AGTACAT114000.017.7370012
GTGGTAT22600.017.4671271
ATGGGGG56950.016.9181615
GTATTAA26500.016.8707261
Produced by FastQC (version 0.11.5)