##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920875_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4422198
Sequences flagged as poor quality	0
Sequence length	101
%GC	48
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.19260919569861	31.0	30.0	34.0	26.0	34.0
2	30.202101081860196	31.0	30.0	34.0	26.0	34.0
3	30.339800027045374	31.0	30.0	34.0	26.0	34.0
4	33.98633032713596	35.0	35.0	37.0	30.0	37.0
5	33.49578377087593	35.0	35.0	37.0	28.0	37.0
6	33.44079618325548	35.0	35.0	37.0	28.0	37.0
7	33.25268859512848	35.0	33.0	37.0	27.0	37.0
8	33.184847896905566	35.0	33.0	37.0	27.0	37.0
9	34.50922098015512	38.0	34.0	39.0	27.0	39.0
10-11	34.315884883490064	37.5	34.0	39.0	27.0	39.0
12-13	34.12854241261924	37.0	34.0	39.0	26.0	39.0
14-15	35.02292231148402	38.0	34.0	40.0	25.5	41.0
16-17	34.90113513687085	38.0	34.0	40.0	25.0	41.0
18-19	34.817821590078054	38.0	33.5	40.0	25.0	41.0
20-21	34.70543878858432	38.0	34.0	40.0	25.0	41.0
22-23	34.556347092554425	38.0	33.5	40.0	24.0	41.0
24-25	34.378967427510034	38.0	33.0	40.0	23.5	41.0
26-27	34.19007063908039	38.0	33.0	40.0	22.5	41.0
28-29	34.131480431224475	38.0	33.0	40.0	22.5	41.0
30-31	34.114722588179	38.0	33.0	40.0	22.0	41.0
32-33	33.96233818567147	38.0	33.0	40.0	21.0	41.0
34-35	33.790757559928345	38.0	33.0	40.0	19.5	41.0
36-37	33.595251614694774	38.0	32.5	40.0	18.5	41.0
38-39	33.412281403953415	38.0	32.0	40.0	18.0	41.0
40-41	33.17909057893835	38.0	32.0	40.0	16.0	41.0
42-43	33.19587024371138	38.0	32.0	40.0	15.5	41.0
44-45	33.0447652954481	38.0	32.0	40.0	15.0	41.0
46-47	32.792079526968266	37.5	31.5	40.0	12.5	41.0
48-49	32.529770489697654	37.0	31.0	40.0	9.0	41.0
50-51	32.27826343370423	37.0	31.0	40.0	8.0	41.0
52-53	32.00956153478428	37.0	31.0	40.0	7.5	41.0
54-55	31.726560072615474	36.0	30.0	39.5	6.5	41.0
56-57	31.404588057793887	36.0	30.0	39.0	2.0	41.0
58-59	31.075943116974862	35.5	29.0	39.0	2.0	40.0
60-61	30.748931639876822	35.0	29.0	39.0	2.0	40.0
62-63	30.426809812676865	35.0	28.5	38.5	2.0	40.0
64-65	30.031654168357	35.0	28.0	38.0	2.0	40.0
66-67	29.615115718473028	34.0	28.0	38.0	2.0	40.0
68-69	29.184651049093688	34.0	28.0	37.0	2.0	39.5
70-71	28.769561539306924	34.0	26.5	36.5	2.0	39.0
72-73	28.34125767322042	34.0	26.0	36.0	2.0	39.0
74-75	27.901461558256777	33.0	26.0	35.5	2.0	38.5
76-77	26.445208468729803	31.5	24.5	34.0	2.0	36.0
78-79	27.293836345636265	33.0	26.0	35.0	2.0	37.0
80-81	27.32696545925804	33.0	26.0	35.0	2.0	36.5
82-83	26.99494708287598	33.0	25.0	35.0	2.0	36.0
84-85	26.676727274536326	33.0	25.0	35.0	2.0	36.0
86-87	26.140777278629315	32.5	24.0	35.0	2.0	35.0
88-89	25.77030393030796	32.0	22.5	35.0	2.0	35.0
90-91	25.406889854321314	32.0	20.0	34.5	2.0	35.0
92-93	25.09886181034861	32.0	19.5	34.0	2.0	35.0
94-95	24.710087608017552	32.0	17.5	34.0	2.0	35.0
96-97	24.172821411433862	31.5	4.5	34.0	2.0	35.0
98-99	23.573201606983673	31.0	2.0	34.0	2.0	35.0
100-101	21.40175157240811	28.0	2.0	33.0	2.0	34.5
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	110317.0
3	48733.0
4	25813.0
5	18126.0
6	15639.0
7	15728.0
8	17625.0
9	20222.0
10	23147.0
11	25751.0
12	28412.0
13	30828.0
14	33778.0
15	35778.0
16	37516.0
17	38884.0
18	37177.0
19	35939.0
20	35914.0
21	37679.0
22	40765.0
23	46164.0
24	54683.0
25	65228.0
26	76768.0
27	91504.0
28	107370.0
29	126705.0
30	149415.0
31	177062.0
32	211269.0
33	258157.0
34	324870.0
35	421446.0
36	558270.0
37	646685.0
38	367072.0
39	25759.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	41.87268692374877	19.572768373486127	14.018938479959854	24.53560622280525
2	16.018301306273486	20.960730387920215	37.96777077824195	25.053197527564347
3	18.7876147964405	24.780765287741108	29.72862276386248	26.70299715195592
4	13.923246474041045	16.771831408931632	36.08695337515498	33.21796874187235
5	14.487060585357755	37.08133446497946	34.05856834100089	14.373036608661888
6	33.148158460793866	35.52349027016969	17.13356541313691	14.19478585589954
7	28.84732095625267	31.84632184032986	21.28411306042885	18.022244142988615
8	26.62502915078939	34.43578788640362	20.21051964253774	18.728663320269252
9	26.796537669029586	15.496587421411279	19.724045255871495	37.982829653687645
10-11	25.68745340512463	25.376907687302623	27.74591397043692	21.18972493713583
12-13	26.223228609035477	22.754171154883203	27.651149323020736	23.371450913060585
14-15	23.523884979176437	24.310834648425292	25.047105026124683	27.11817534627359
16-17	23.529572825648653	27.19333657070265	25.870785970212886	23.40630463343581
18-19	23.558013770484607	26.505585921140906	27.182636067752313	22.75376424062217
20-21	24.370999877278198	25.70652976116754	26.82273260163995	23.09973775991431
22-23	24.13822174141334	25.48838061957958	26.70360621752277	23.669791421484312
24-25	23.84710624311875	25.71803716972824	26.678595299412756	23.75626128774025
26-27	23.643018805419374	25.860110452931238	26.666354397050362	23.830516344599026
28-29	23.754352515772215	26.02647207331772	26.53141140956359	23.687764001346476
30-31	24.01329528111388	25.85299983544884	26.282597103560356	23.85110777987692
32-33	23.806431816405603	25.881401021559842	26.226644862110003	24.08552229992455
34-35	24.150491681476577	25.88651660170662	26.287506580349817	23.675485136466985
36-37	24.31084760426474	25.5939336878801	26.254646004563302	23.84057270329186
38-39	24.077277343847197	25.679657439892512	26.284724814118615	23.95834040214168
40-41	24.21063429050558	25.54250479460715	26.410374491672115	23.83648642321515
42-43	24.097508307732877	25.525340352157183	26.158092931040077	24.21905840906986
44-45	23.890360391130574	25.53183307962172	26.374159415339864	24.203647113907845
46-47	24.007718199750265	25.547116759260934	26.245430608588645	24.199734432400152
48-49	23.835417641710336	25.671034523076013	26.12678675397781	24.36676108123584
50-51	23.78827973724382	25.72965117035151	26.223984162889018	24.25808492951565
52-53	23.94897930569087	25.47881088004863	26.20814800542392	24.364061808836578
54-55	23.570367316374888	25.47353630199033	26.426596487760285	24.529499893874494
56-57	23.536970278638194	25.513215713083596	26.314012461863722	24.635801546414484
58-59	23.54578433012067	25.39172323851605	26.2773116835554	24.785180747807882
60-61	23.4425138633835	25.356516892892145	26.481703183698286	24.719266060026072
62-63	23.372125118688345	25.38123029705642	26.42643403280067	24.820210551454572
64-65	23.167322117917905	25.260341166333856	26.367740943352274	25.204595772395965
66-67	23.18055722868569	25.383105325757022	26.256077704122347	25.18025974143494
68-69	23.277097176784693	25.263379230330134	26.235923419600134	25.223600173285043
70-71	23.04123851829535	25.12477046768894	26.2515186707013	25.582472343314404
72-73	22.978220248096033	25.348744005763564	26.147262890782702	25.525772855357705
74-75	22.982573096251908	25.37549202256624	26.08406788974983	25.55786699143202
76-77	22.999825101127932	25.085576329188918	26.272270276905836	25.64232829277731
78-79	22.882571893938998	25.206823296014804	26.22113158680287	25.689473223243333
80-81	22.8811692459122	25.26919914019118	26.10052182000105	25.749109793895574
82-83	22.73735579433109	25.28025832718088	26.17120937869875	25.81117649978928
84-85	22.653522455698898	25.229141284738137	26.049689514039777	26.067646745523188
86-87	22.5885943317993	25.298003109637097	26.02604136848973	26.087361190073878
88-89	22.47805897254849	25.093642887520907	26.106733663722082	26.321564476208533
90-91	22.344162674907416	25.16720268644368	26.033329323907665	26.45530531474124
92-93	22.464815540788557	25.00411031413693	26.027473577970305	26.503600567104215
94-95	22.21486783241911	24.83159213694874	26.186102645522507	26.767437385109645
96-97	22.265765675614954	24.757930171949717	26.16943457621569	26.806869576219643
98-99	22.10688005137877	24.730668511960488	26.091131734983243	27.0713197016775
100-101	22.176151859574215	24.702723666773707	26.140665493738098	26.980458979913973
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	127.0
1	105.5
2	116.5
3	164.0
4	160.5
5	175.5
6	280.5
7	601.5
8	892.5
9	912.0
10	791.0
11	686.0
12	703.0
13	725.0
14	780.5
15	890.5
16	968.0
17	1064.5
18	1224.5
19	1378.5
20	1608.0
21	1857.0
22	2171.5
23	2585.0
24	3163.0
25	3956.5
26	5095.5
27	6869.0
28	9373.5
29	13119.5
30	18554.5
31	26182.5
32	35896.5
33	45028.5
34	52939.5
35	62518.5
36	74834.5
37	87009.5
38	97796.5
39	108078.0
40	118261.5
41	127775.5
42	136735.0
43	146233.0
44	155882.0
45	165450.0
46	175318.5
47	184335.0
48	191372.5
49	195826.5
50	196428.0
51	196054.0
52	194456.0
53	192745.5
54	189459.0
55	179043.0
56	164672.0
57	147625.5
58	132307.5
59	117572.0
60	99040.5
61	81996.5
62	67095.0
63	51277.5
64	37227.0
65	27713.5
66	20910.5
67	15528.5
68	11262.0
69	8295.5
70	6270.0
71	4719.5
72	3495.0
73	2729.5
74	2048.5
75	1445.5
76	989.0
77	589.5
78	321.5
79	157.0
80	91.5
81	52.5
82	29.5
83	21.5
84	10.5
85	3.5
86	1.0
87	0.5
88	0.5
89	0.5
90	1.0
91	0.5
92	0.0
93	0.5
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.19069702442088754
2	0.0
3	0.01282167826949404
4	0.012753838701930578
5	0.007258833729290276
6	0.10745787502052147
7	0.14232741274814018
8	0.12455344604651351
9	0.1431188743697139
10-11	0.1161187264794566
12-13	0.11478454831737521
14-15	0.11773556950638574
16-17	0.06720639826620155
18-19	0.08015244907622861
20-21	0.03687080497074079
22-23	0.0840758374003154
24-25	0.05834202810457605
26-27	0.06648277621219131
28-29	0.03993489210569043
30-31	0.024433550917439695
32-33	0.031873290160232534
34-35	0.020137044971753865
36-37	0.023981287133683294
38-39	0.030934842808938
40-41	0.02453531026878489
42-43	0.012765145296524488
44-45	0.026412204971373962
46-47	0.03342229361959822
48-49	0.008762610810280317
50-51	0.01547872799906291
52-53	0.006919635891472973
54-55	0.012358107891143725
56-57	0.00513319394563518
58-59	0.0037650959997720594
60-61	0.023042839782388757
62-63	0.009689751566980945
64-65	0.0069648622698486136
66-67	0.04161957470018303
68-69	0.09144773707554478
70-71	0.02214961880946986
72-73	0.04555426961886374
74-75	0.08657459480556953
76-77	0.250497603228078
78-79	0.08075169858970584
80-81	0.016959891890865133
82-83	0.01172493859388476
84-85	0.013138262918123523
86-87	0.26492481792990724
88-89	0.6236039182325169
90-91	0.2658745718757957
92-93	0.35277705792458863
94-95	0.001062819891827548
96-97	0.004036454270025901
98-99	0.003120620107919184
100-101	0.0049296752429447985
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4422198.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.25556082625481
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.05191773338723	35.48957616565162
2	13.053383318290077	12.859034320449133
3	5.575037695714098	8.238048249897277
4	2.8253816595221184	5.566630327519058
5	1.6749421719618605	4.125010801576337
6	1.0849528475943693	3.2063976586981693
7	0.7325693952852801	2.525818148624877
8	0.5246186713580858	2.067230950213374
9	0.3749968103813444	1.6623610383050866
>10	1.95829828733812	16.986692560773918
>50	0.1082114579203097	3.6090782619939907
>100	0.03379939271905526	2.719379876933873
>500	0.0012911132862138148	0.461128282135854
>1k	5.994452418445909E-4	0.4836133572274951
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.2613189187820173E-5
2	0.0	0.0	0.0	0.0	2.2613189187820173E-5
3	0.0	4.5226378375640346E-5	0.0	0.0	2.2613189187820173E-5
4	0.0	4.5226378375640346E-5	0.0	2.2613189187820173E-5	2.2613189187820173E-5
5	0.0	6.783956756346053E-5	0.0	2.2613189187820173E-5	2.2613189187820173E-5
6	0.0	6.783956756346053E-5	0.0	2.2613189187820173E-5	2.2613189187820173E-5
7	0.0	6.783956756346053E-5	0.0	2.2613189187820173E-5	2.2613189187820173E-5
8	0.0	6.783956756346053E-5	0.0	2.2613189187820173E-5	2.2613189187820173E-5
9	0.0	6.783956756346053E-5	0.0	2.2613189187820173E-5	2.2613189187820173E-5
10-11	0.0	1.1306594593910087E-4	0.0	2.2613189187820173E-5	4.5226378375640346E-5
12-13	0.0	1.3567913512692106E-4	0.0	2.2613189187820173E-5	1.6959891890865132E-4
14-15	0.0	1.4698572972083115E-4	0.0	2.2613189187820173E-5	2.2613189187820174E-4
16-17	0.0	1.695989189086513E-4	0.0	2.2613189187820173E-5	2.9397145944166225E-4
18-19	0.0	1.8090551350256139E-4	0.0	4.522637837564035E-5	3.3919783781730263E-4
20-21	0.0	2.4874508106602193E-4	0.0	1.3567913512692103E-4	4.0703740538076315E-4
22-23	0.0	2.4874508106602193E-4	0.0	2.7135827025384206E-4	4.522637837564035E-4
24-25	0.0	2.826648648477522E-4	0.0	0.001051513297233638	4.7487697294422367E-4
26-27	0.0	2.9397145944166225E-4	0.0	0.002035187026903816	5.540231351015943E-4
28-29	0.0	3.052780540355723E-4	0.0	0.006851796323909513	5.653297296955044E-4
30-31	0.0	3.957308107868531E-4	0.0	0.028990108538785465	5.879429188833246E-4
32-33	0.0	4.635703783503136E-4	0.0	0.08161099977884301	6.44475891852875E-4
34-35	0.0	4.7487697294422367E-4	0.0	0.15239028193672016	6.55782486446785E-4
36-37	0.0	4.8618356753813373E-4	0.0	0.27475024863201514	6.55782486446785E-4
38-39	0.0	5.879429188833246E-4	0.0	0.4723329891605939	6.55782486446785E-4
40-41	0.0	6.105561080711448E-4	0.0	0.6998555921738465	6.55782486446785E-4
42-43	0.0	6.783956756346053E-4	0.0	0.9432413474023551	6.783956756346052E-4
44-45	0.0	7.688484323858859E-4	0.0	1.2419728831680534	7.236220540102456E-4
46-47	0.0	7.80155026979796E-4	0.0	1.5957336148223122	7.80155026979796E-4
48-49	0.0	8.027682161676162E-4	0.0	1.9670534878809134	8.366879999493464E-4
50-51	0.0	8.140748107615263E-4	0.0	2.3673521628837064	8.593011891371667E-4
52-53	0.0	8.366879999493464E-4	0.0	2.7688606435080474	0.001028900108045818
54-55	0.0	8.593011891371667E-4	0.0	3.2192814523456432	0.0011080462702031885
56-57	0.0	8.819143783249868E-4	0.0	3.7410694862600002	0.0011306594593910087
58-59	0.0	9.271407567006272E-4	0.0	4.297557458983066	0.001153272648578829
60-61	0.0	9.384473512945373E-4	0.0	4.894918771163118	0.001153272648578829
62-63	0.0	9.497539458884473E-4	0.0	5.496395683775353	0.0012437254053301095
64-65	0.0	0.001017593513451908	0.0	6.141968315303838	0.00127764518911184
66-67	2.2613189187820173E-5	0.001040206702639728	0.0	6.874364286718958	0.0013002583782996601
68-69	4.5226378375640346E-5	0.001040206702639728	0.0	7.675696565373147	0.00131156497289357
70-71	4.5226378375640346E-5	0.001051513297233638	0.0	8.522549193862417	0.00131156497289357
72-73	4.5226378375640346E-5	0.0010854330810153685	0.0	9.37677372202692	0.0013794045404570306
74-75	4.5226378375640346E-5	0.0010854330810153685	0.0	10.26118685775716	0.001424630918832671
76-77	4.5226378375640346E-5	0.0010854330810153685	0.0	11.220698394780154	0.0014698572972083113
78-79	4.5226378375640346E-5	0.0010854330810153685	0.0	12.266060904554703	0.0015037770809900415
80-81	4.5226378375640346E-5	0.0010854330810153685	0.0	13.343726355084055	0.0015716166485535022
82-83	4.5226378375640346E-5	0.0010854330810153685	0.0	14.448007529287473	0.0016507628107108728
84-85	4.5226378375640346E-5	0.0010854330810153685	0.0	15.55102236489637	0.0016733759998986929
86-87	4.5226378375640346E-5	0.0010854330810153685	0.0	16.69391555963799	0.0017186023782743334
88-89	4.5226378375640346E-5	0.0010854330810153685	0.0	17.897536473943504	0.0017412155674621534
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	4325	0.0	50.255302	1
GTATCAA	7485	0.0	43.20847	1
TCAACGC	10380	0.0	30.182245	4
TATCAAC	10495	0.0	30.030397	2
ATCAACG	10555	0.0	29.7268	3
CAACGCA	10805	0.0	28.859676	5
AACGCAG	11175	0.0	28.116505	6
GTACATG	14515	0.0	26.213482	1
TACATGG	15095	0.0	24.306467	2
ACGCAGA	13125	0.0	23.469091	7
ACATGGG	15350	0.0	23.038427	3
CGCAGAG	13440	0.0	22.777002	8
CATGGGG	11330	0.0	20.361572	4
GAGTACA	11330	0.0	19.519775	1
GCAGAGT	14890	0.0	19.284204	9
AGAGTAC	12720	0.0	18.524485	10-11
AGTACAT	11400	0.0	17.737001	2
GTGGTAT	2260	0.0	17.467127	1
ATGGGGG	5695	0.0	16.918161	5
GTATTAA	2650	0.0	16.870726	1
>>END_MODULE