FastQCFastQC Report
Mon 15 Aug 2016
SRR2920874_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920874_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3070620
Sequences flagged as poor quality0
Sequence length98
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25150.043.1852571
GTATCAA44000.042.6745571
TCAACGC54500.034.0880784
CAACGCA56350.033.050355
ATCAACG56450.032.9918023
TATCAAC59500.031.1479592
AACGCAG60550.031.1320676
GTACATG93500.026.6285291
TACATGG96550.025.2769412
ACGCAGA73800.025.0401547
ACATGGG98550.024.2507423
CGCAGAG79000.023.3323768
TATACCG2254.2164174E-922.4261195
CATGGGG70200.020.975484
GAGTACA70600.020.9248261
GCAGAGT87500.020.0177549
AGTACAT69600.019.9709432
AGAGTAC74200.017.70452510-11
GTATAGG9150.016.5979461
TACACCG4551.6370905E-1116.1306765
Produced by FastQC (version 0.11.5)