##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920874_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3070620
Sequences flagged as poor quality	0
Sequence length	98
%GC	48
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.104244419693742	31.0	30.0	33.0	25.0	34.0
2	29.15287010440888	31.0	30.0	34.0	23.0	34.0
3	29.227866033569768	31.0	30.0	34.0	23.0	34.0
4	32.758302883456764	35.0	33.0	37.0	26.0	37.0
5	32.734863968840166	35.0	33.0	37.0	26.0	37.0
6	32.854133367202714	35.0	33.0	37.0	26.0	37.0
7	32.698800893630604	35.0	33.0	37.0	25.0	37.0
8	32.651298760510905	35.0	33.0	37.0	25.0	37.0
9	33.973816362819235	37.0	34.0	39.0	25.0	39.0
10-11	33.800168044238625	37.0	33.0	39.0	25.0	39.0
12-13	33.63945962053266	37.0	33.0	39.0	24.0	39.0
14-15	34.46145957493927	38.0	33.0	40.0	24.0	41.0
16-17	34.36212149337919	38.0	33.0	40.0	23.0	41.0
18-19	34.254875236922835	38.0	33.0	40.0	22.0	41.0
20-21	34.16991454494532	38.0	33.0	40.0	21.5	41.0
22-23	33.95828920543734	38.0	33.0	40.0	19.0	41.0
24-25	33.77130546925377	38.0	33.0	40.0	18.0	41.0
26-27	33.82220170519309	38.0	33.0	40.0	18.0	41.0
28-29	33.678233874592095	38.0	33.0	40.0	17.5	41.0
30-31	33.44111970872332	38.0	32.0	40.0	16.5	41.0
32-33	33.215178042219485	38.0	32.0	40.0	16.0	41.0
34-35	32.90235229367359	38.0	31.5	40.0	12.0	41.0
36-37	32.61622815587731	38.0	31.0	40.0	9.0	41.0
38-39	32.425322410457824	37.0	31.0	40.0	9.0	41.0
40-41	32.239722759572985	37.0	30.5	40.0	8.5	41.0
42-43	32.01967762210889	37.0	30.0	40.0	8.0	41.0
44-45	31.757640476516144	37.0	30.0	40.0	4.5	41.0
46-47	31.52159140499313	37.0	30.0	40.0	2.0	41.0
48-49	31.23406787554305	36.0	30.0	39.0	2.0	40.5
50-51	30.084359021956477	34.5	28.0	38.0	2.0	39.5
52-53	30.548039158215605	35.5	29.0	38.5	2.0	39.5
54-55	31.225675596459347	36.0	30.0	39.0	2.0	40.5
56-57	31.19135598022549	36.0	30.0	39.5	2.0	41.0
58-59	30.959894255883178	36.0	29.5	39.0	2.0	41.0
60-61	30.649695826901407	35.5	29.0	39.0	2.0	40.5
62-63	30.265090437761756	35.0	28.5	39.0	2.0	40.0
64-65	29.855608313630473	35.0	28.0	38.5	2.0	40.0
66-67	29.476537148849417	34.5	28.0	38.0	2.0	40.0
68-69	29.029562433645324	34.0	27.0	37.5	2.0	40.0
70-71	28.702286346079944	34.0	26.5	37.0	2.0	39.5
72-73	28.233029974402562	34.0	26.0	36.0	2.0	39.0
74-75	27.793556187349786	34.0	26.0	36.0	2.0	39.0
76-77	27.37583712735539	33.0	25.5	35.5	2.0	38.0
78-79	26.916262513759435	33.0	25.0	35.0	2.0	37.0
80-81	26.467913320436914	33.0	24.0	35.0	2.0	37.0
82-83	25.99779181403104	32.0	22.5	35.0	2.0	36.0
84-85	25.597110355563373	32.0	20.5	35.0	2.0	36.0
86-87	25.152493633207627	32.0	19.0	35.0	2.0	35.0
88-89	24.62726468921586	31.5	16.0	34.0	2.0	35.0
90-91	24.138921129934673	31.0	7.5	34.0	2.0	35.0
92-93	23.606494128221662	31.0	2.0	34.0	2.0	35.0
94-95	22.97726859722141	31.0	2.0	34.0	2.0	35.0
96-97	22.33887439670164	30.5	2.0	34.0	2.0	35.0
98	21.947528837824283	30.0	2.0	34.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	127558.0
3	22293.0
4	16473.0
5	13526.0
6	12871.0
7	12708.0
8	14497.0
9	16713.0
10	18432.0
11	19888.0
12	21147.0
13	21327.0
14	20117.0
15	20837.0
16	22769.0
17	24729.0
18	25353.0
19	25240.0
20	26238.0
21	28307.0
22	30452.0
23	34274.0
24	39700.0
25	45736.0
26	53690.0
27	62311.0
28	72538.0
29	84584.0
30	100210.0
31	119339.0
32	143344.0
33	176999.0
34	223722.0
35	285991.0
36	368498.0
37	421179.0
38	267281.0
39	29748.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.438299153420786	17.983344300062793	12.599023356355293	24.979333190161128
2	16.202353683102316	19.35749298351223	38.93349385910736	25.50665947427809
3	19.691709263151655	23.19079349165448	28.700503470746284	28.41699377444758
4	13.548281396978929	14.981199725878195	36.044509616411865	35.42600926073101
5	14.676401379033582	36.61012333646908	34.01570566023969	14.697769624257647
6	34.34646307435423	34.28493064653676	16.930570964902735	14.438035314206271
7	29.794559811972427	31.142750867373593	20.542846236126255	18.519843084527725
8	26.96995199902031	34.33490079645539	19.81481614172177	18.88033106280253
9	26.696921142961354	15.742944421647746	19.489158541271795	38.0709758941191
10-11	25.899598745399544	25.195750433381676	27.32696567043974	21.577685150779043
12-13	26.302708112580543	22.407427943602578	27.414040146108427	23.875823797708453
14-15	23.348289496695845	24.11397189213976	24.842120038599212	27.695618572565184
16-17	23.649762909461362	27.25353258307451	25.51343019096703	23.583274316497103
18-19	23.648067851573707	26.445521593172362	26.925140009516134	22.9812705457378
20-21	24.1577006028617	25.81022119051945	26.818668098520916	23.213410108097936
22-23	23.94532858233946	25.406973086430295	26.687912706192147	23.959785625038098
24-25	23.68086218164452	25.76985450261114	26.736247061236906	23.813036254507434
26-27	23.54059689507146	25.99952353204198	26.67472621016995	23.78515336271661
28-29	23.55998039030718	26.125353771990234	26.589967937924147	23.724697899778437
30-31	23.802321360728456	25.963959020704046	26.39884759578008	23.83487202278742
32-33	23.54938844644224	26.012898812722916	26.383511669191055	24.054201071643785
34-35	23.896971972290864	25.975467463397408	26.466758612216868	23.660801952094864
36-37	23.97735094642722	25.802239899032053	26.411016268185637	23.809392886355088
38-39	23.70984909914073	25.92437797500793	26.51836945478315	23.847403471068187
40-41	23.938859625214842	25.823795418725386	26.54417367866032	23.693171277399454
42-43	23.796250269800055	25.784774774185532	26.428403241525007	23.99057171448941
44-45	23.662339394739924	25.82374665940405	26.50558133725846	24.00833260859757
46-47	23.716910564507206	25.791651048257492	26.450885504541503	24.0405528826938
48-49	23.638545039662755	25.85243460508056	26.437936327096374	24.07108402816031
50-51	23.6513324410021	25.966185015929078	26.430531784846075	23.951950758222747
52-53	23.75810196599476	25.844320900246977	26.480539172129582	23.917037961628683
54-55	23.382799625535544	25.754571390627788	26.708265105656675	24.15436387817999
56-57	23.444195105601132	25.821808564993642	26.614918637874162	24.119077691531064
58-59	23.35714062683657	25.621375591728867	26.694510230695172	24.326973550739385
60-61	23.390867329654334	25.62081517921726	26.85704998829329	24.131267502835115
62-63	23.29585662720107	25.62970887044978	26.882089205534914	24.19234529681424
64-65	23.194760276123386	25.553908433381835	26.8054523378377	24.445878952657086
66-67	23.151228653677975	25.547846809334818	26.740888522608934	24.56003601437827
68-69	23.273861168618147	25.482114944422946	26.680599145642468	24.56342474131644
70-71	23.189634533469842	25.20603709819886	26.864171827365336	24.740156540965966
72-73	23.231885850626007	25.287333408388037	26.75220723724469	24.72857350374127
74-75	23.096135344544084	25.432402632930184	26.71895474808486	24.752507274440873
76-77	23.11389561644619	25.248747176808372	26.804020469926115	24.83333673681932
78-79	23.13500023769977	25.17397417845323	26.83150124804633	24.85952433580067
80-81	23.171650692295376	25.194845936910763	26.745607184055476	24.887896186738384
82-83	23.19198181320895	25.150519092764856	26.819651249917083	24.837847844109113
84-85	23.061448092556418	25.172435540085626	26.839851594987902	24.926264772370054
86-87	23.083426191739147	25.082992625618594	26.866543806596184	24.967037376046076
88-89	23.049942929463953	24.90050245605961	26.851736238679045	25.197818375797386
90-91	23.111989687583385	24.91939135304063	26.86917861614728	25.0994403432287
92-93	23.25798919758961	24.65487852557342	26.86698892980851	25.220143347028458
94-95	23.1569274548294	24.57430604868239	26.998752663701858	25.270013832786354
96-97	23.28265889965861	24.36683100646608	27.014959856188785	25.335550237686522
98	23.309056878009503	24.280692375659413	26.90206593854728	25.508184807783802
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	50.0
1	44.5
2	69.5
3	118.0
4	133.5
5	156.5
6	262.0
7	476.0
8	757.5
9	897.0
10	888.5
11	969.0
12	1104.0
13	1248.0
14	1402.0
15	1534.0
16	1657.5
17	1744.0
18	1763.0
19	1796.0
20	1869.0
21	1899.0
22	2014.0
23	2377.5
24	2859.0
25	3586.5
26	4859.5
27	6676.5
28	9399.0
29	13242.0
30	18516.0
31	25268.0
32	31425.0
33	37322.5
34	44677.0
35	52903.0
36	61221.5
37	69562.0
38	77048.0
39	83812.5
40	90616.5
41	97814.0
42	106375.5
43	114047.5
44	120030.5
45	125550.5
46	130658.5
47	135157.0
48	137754.0
49	137550.5
50	136733.0
51	135456.5
52	132582.5
53	129724.0
54	125174.5
55	118253.0
56	108513.5
57	97394.0
58	87438.0
59	75820.0
60	63823.0
61	53152.0
62	43309.5
63	33733.5
64	24945.0
65	18737.0
66	14272.5
67	11016.5
68	8425.0
69	6393.0
70	4817.0
71	3571.5
72	2869.0
73	2317.5
74	1803.0
75	1374.0
76	983.5
77	612.5
78	360.0
79	201.5
80	132.5
81	90.5
82	54.5
83	36.0
84	21.0
85	13.5
86	11.0
87	8.5
88	8.0
89	7.5
90	4.0
91	2.5
92	3.5
93	3.5
94	3.0
95	4.5
96	4.0
97	1.5
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3701858256638724
2	0.023057232741270493
3	0.017292924555952868
4	0.014720154235952349
5	0.020972963114940957
6	0.022601298760510907
7	0.014068819977724368
8	0.008662745634432133
9	0.0
10-11	3.2566712911399E-5
12-13	0.005226957422279539
14-15	0.0029635708749373092
16-17	0.0054874911255707315
18-19	0.035709400707349
20-21	0.07612469143039516
22-23	0.09480170128508249
24-25	0.06316313969165836
26-27	0.07190730210836899
28-29	0.05632412998026457
30-31	0.050738938715959636
32-33	0.018563026359497428
34-35	0.32934716767297806
36-37	0.5012994118451648
38-39	0.45627593124515575
40-41	0.4659970950492083
42-43	0.4168539252659072
44-45	0.39190782317577555
46-47	0.39068657144159813
48-49	0.3811933746279253
50-51	0.3774970527124815
52-53	0.4368661703499619
54-55	0.4381525555099621
56-57	0.40814232956210794
58-59	0.3941712097231178
60-61	0.4085168467605891
62-63	0.38964443662843334
64-65	0.43756635467755695
66-67	0.45798568367300413
68-69	0.4005217187408406
70-71	0.35945509375956647
72-73	0.3461678748917157
74-75	0.32812591593880064
76-77	0.3267255472836105
78-79	0.32685581413525605
80-81	0.3422761526988035
82-83	0.33794477988158744
84-85	0.33693521178133407
86-87	0.32425047710234417
88-89	0.36631038682741596
90-91	0.34584220776260166
92-93	0.33493235893728307
94-95	0.34191791885677814
96-97	0.3418527854309553
98	0.3359582103939921
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
98	3070620.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.22133119845478
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.39341649991897	39.79488743828243
2	12.799977911833633	13.88063683380874
3	5.4473609037132835	8.860894791532555
4	2.7534187740565876	5.9717612510466225
5	1.6289317810784718	4.416142480077233
6	0.9893927451623371	3.218771503247727
7	0.6506763679535528	2.4696377194872055
8	0.4628534555812859	2.007722440913774
9	0.3411421162086971	1.6647461701824164
>10	1.4507224418032416	13.273491545817018
>50	0.06073246080336742	2.223939939495444
>100	0.020468739032141477	1.7882111821984237
>500	7.246422836909895E-4	0.2900008336805766
>1k	1.8116057081628934E-4	0.13915587022969125
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	3.2566712911399E-5	0.0	0.0	0.0
3	0.0	3.2566712911399E-5	0.0	0.0	0.0
4	0.0	3.2566712911399E-5	0.0	3.2566712911399E-5	0.0
5	0.0	3.2566712911399E-5	0.0	3.2566712911399E-5	0.0
6	0.0	3.2566712911399E-5	0.0	3.2566712911399E-5	0.0
7	0.0	3.2566712911399E-5	0.0	6.5133425822798E-5	0.0
8	0.0	3.2566712911399E-5	0.0	6.5133425822798E-5	0.0
9	0.0	3.2566712911399E-5	0.0	9.7700138734197E-5	0.0
10-11	0.0	3.2566712911399E-5	0.0	9.7700138734197E-5	0.0
12-13	0.0	3.2566712911399E-5	0.0	9.7700138734197E-5	0.0
14-15	0.0	3.2566712911399E-5	0.0	9.7700138734197E-5	0.0
16-17	0.0	3.2566712911399E-5	0.0	1.139834951898965E-4	0.0
18-19	0.0	3.2566712911399E-5	0.0	1.30266851645596E-4	0.0
20-21	0.0	3.2566712911399E-5	0.0	2.442503468354925E-4	0.0
22-23	0.0	3.2566712911399E-5	0.0	4.396506243038865E-4	0.0
24-25	3.2566712911399E-5	4.88500693670985E-5	0.0	8.46734535696374E-4	0.0
26-27	3.2566712911399E-5	6.5133425822798E-5	0.0	0.00130266851645596	0.0
28-29	3.2566712911399E-5	6.5133425822798E-5	0.0	0.00522695742227954	0.0
30-31	3.2566712911399E-5	6.5133425822798E-5	0.0	0.022747848968612202	0.0
32-33	3.2566712911399E-5	6.5133425822798E-5	0.0	0.059743634835961464	0.0
34-35	3.2566712911399E-5	6.5133425822798E-5	0.0	0.11155727507799729	0.0
36-37	3.2566712911399E-5	6.5133425822798E-5	0.0	0.19999218398890126	0.0
38-39	3.2566712911399E-5	6.5133425822798E-5	0.0	0.35002703037171645	0.0
40-41	3.2566712911399E-5	6.5133425822798E-5	0.0	0.5176804684395985	0.0
42-43	3.2566712911399E-5	6.5133425822798E-5	0.0	0.6956901212133055	0.0
44-45	3.2566712911399E-5	6.5133425822798E-5	0.0	0.9157922504249956	0.0
46-47	3.2566712911399E-5	6.5133425822798E-5	0.0	1.1830998300017586	0.0
48-49	3.2566712911399E-5	6.5133425822798E-5	0.0	1.4655834977952336	0.0
50-51	3.2566712911399E-5	6.5133425822798E-5	0.0	1.7771166735056765	0.0
52-53	3.2566712911399E-5	6.5133425822798E-5	0.0	2.095293458650045	0.0
54-55	3.2566712911399E-5	6.5133425822798E-5	0.0	2.454536868775687	0.0
56-57	3.2566712911399E-5	6.5133425822798E-5	0.0	2.872286378646658	0.0
58-59	3.2566712911399E-5	6.5133425822798E-5	0.0	3.327487608365737	3.2566712911399E-5
60-61	3.2566712911399E-5	8.14167822784975E-5	0.0	3.81523926763976	3.2566712911399E-5
62-63	3.2566712911399E-5	9.7700138734197E-5	0.0	4.312923774351759	3.2566712911399E-5
64-65	3.2566712911399E-5	9.7700138734197E-5	0.0	4.8451289967498425	3.2566712911399E-5
66-67	3.2566712911399E-5	9.7700138734197E-5	0.0	5.447841152601104	3.2566712911399E-5
68-69	3.2566712911399E-5	9.7700138734197E-5	0.0	6.118699155219467	3.2566712911399E-5
70-71	3.2566712911399E-5	9.7700138734197E-5	0.0	6.837202258827207	3.2566712911399E-5
72-73	3.2566712911399E-5	9.7700138734197E-5	0.0	7.554777211116972	3.2566712911399E-5
74-75	3.2566712911399E-5	9.7700138734197E-5	0.0	8.29226670835206	3.2566712911399E-5
76-77	3.2566712911399E-5	9.7700138734197E-5	0.0	9.097560101868678	3.2566712911399E-5
78-79	4.88500693670985E-5	9.7700138734197E-5	0.0	9.986826764627338	3.2566712911399E-5
80-81	6.5133425822798E-5	9.7700138734197E-5	0.0	10.90774827233588	3.2566712911399E-5
82-83	6.5133425822798E-5	9.7700138734197E-5	0.0	11.844643752727462	3.2566712911399E-5
84-85	6.5133425822798E-5	9.7700138734197E-5	0.0	12.776035458637018	3.2566712911399E-5
86	6.5133425822798E-5	9.7700138734197E-5	0.0	13.497762666822988	3.2566712911399E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2515	0.0	43.185257	1
GTATCAA	4400	0.0	42.674557	1
TCAACGC	5450	0.0	34.088078	4
CAACGCA	5635	0.0	33.05035	5
ATCAACG	5645	0.0	32.991802	3
TATCAAC	5950	0.0	31.147959	2
AACGCAG	6055	0.0	31.132067	6
GTACATG	9350	0.0	26.628529	1
TACATGG	9655	0.0	25.276941	2
ACGCAGA	7380	0.0	25.040154	7
ACATGGG	9855	0.0	24.250742	3
CGCAGAG	7900	0.0	23.332376	8
TATACCG	225	4.2164174E-9	22.426119	5
CATGGGG	7020	0.0	20.97548	4
GAGTACA	7060	0.0	20.924826	1
GCAGAGT	8750	0.0	20.017754	9
AGTACAT	6960	0.0	19.970943	2
AGAGTAC	7420	0.0	17.704525	10-11
GTATAGG	915	0.0	16.597946	1
TACACCG	455	1.6370905E-11	16.130676	5
>>END_MODULE