FastQCFastQC Report
Mon 15 Aug 2016
SRR2920874_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920874_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3070620
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA46850.044.0507051
GGTATCA26600.041.2955321
TCAACGC59350.034.234634
CAACGCA60100.033.724575
ATCAACG60750.033.367543
TATCAAC64850.031.549222
AACGCAG65500.031.089166
GTACATG96100.026.5719641
ACGCAGA77800.025.86947
TACATGG98850.025.2598292
CGCAGAG81400.024.6637678
ACATGGG99750.023.6053983
GCAGAGT91950.020.5951359
GAGTACA73200.019.8784731
ATACCGT4150.019.4443666
CATGGGG69850.019.3016534
AGAGTAC79550.018.7024410-11
AGTACAT74450.017.9806652
TAAGGTG12350.016.9113675
ATGGGGG36100.016.8304525
Produced by FastQC (version 0.11.5)