##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920874_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3070620
Sequences flagged as poor quality	0
Sequence length	101
%GC	47
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.772702255570536	31.0	30.0	33.0	26.0	34.0
2	29.79472191283845	31.0	30.0	34.0	26.0	34.0
3	29.934946036956706	31.0	30.0	34.0	26.0	34.0
4	33.56050048524402	35.0	33.0	37.0	28.0	37.0
5	33.02641909451511	35.0	33.0	37.0	26.0	37.0
6	32.95576561085383	35.0	33.0	37.0	26.0	37.0
7	32.75128573382574	35.0	33.0	37.0	26.0	37.0
8	32.67305365040285	35.0	33.0	37.0	25.0	37.0
9	33.94529606398708	37.0	33.0	39.0	25.0	39.0
10-11	33.72872612045776	37.0	33.0	39.0	25.0	39.0
12-13	33.52885394480593	37.0	33.0	39.0	23.0	39.0
14-15	34.345459060385195	38.0	33.0	40.0	23.0	41.0
16-17	34.20094817984642	38.0	33.0	40.0	22.0	41.0
18-19	34.09633412796113	38.0	33.0	40.0	21.5	41.0
20-21	33.96502497866881	38.0	33.0	40.0	19.0	41.0
22-23	33.80814118972715	38.0	32.0	40.0	18.5	41.0
24-25	33.603626954816946	38.0	32.0	40.0	18.0	41.0
26-27	33.39819271026698	38.0	32.0	40.0	17.5	41.0
28-29	33.32686639831695	38.0	32.0	40.0	17.0	41.0
30-31	33.30760123362708	38.0	32.0	40.0	16.5	41.0
32-33	33.143139984758776	38.0	32.0	40.0	16.0	41.0
34-35	32.95765415453557	38.0	31.0	40.0	15.5	41.0
36-37	32.74303332877399	37.5	31.0	40.0	13.5	41.0
38-39	32.55083696452182	37.0	31.0	40.0	9.0	41.0
40-41	32.297160833968384	37.0	30.5	40.0	9.0	41.0
42-43	32.308134025050315	37.0	31.0	40.0	9.0	41.0
44-45	32.145689795546176	37.0	30.0	40.0	8.0	41.0
46-47	31.87044945320489	37.0	30.0	40.0	6.5	41.0
48-49	31.581786251636476	37.0	30.0	40.0	2.0	41.0
50-51	31.298215018465328	36.0	30.0	40.0	2.0	41.0
52-53	31.018929890380445	36.0	29.0	39.0	2.0	41.0
54-55	30.715841100494362	35.5	28.5	39.0	2.0	40.0
56-57	30.374561163543518	35.0	28.0	39.0	2.0	40.0
58-59	30.026379037458234	35.0	28.0	39.0	2.0	40.0
60-61	29.67689163751946	35.0	27.5	38.5	2.0	40.0
62-63	29.336015853475846	34.0	27.0	38.0	2.0	40.0
64-65	28.92301571018231	34.0	26.0	38.0	2.0	40.0
66-67	28.472875184816097	34.0	26.0	37.0	2.0	40.0
68-69	28.014815249037653	33.5	26.0	37.0	2.0	39.0
70-71	27.566107333372415	33.0	25.0	36.0	2.0	39.0
72-73	27.10842142629176	33.0	24.0	36.0	2.0	39.0
74-75	26.65958666327973	32.0	22.5	35.0	2.0	38.0
76-77	25.336715223635615	30.5	21.0	34.0	2.0	36.0
78-79	26.163363913476758	32.0	22.5	35.0	2.0	37.0
80-81	26.177214862145107	32.0	23.0	35.0	2.0	36.0
82-83	25.817175521555907	32.0	21.5	35.0	2.0	36.0
84-85	25.47753955227283	32.0	20.0	35.0	2.0	35.5
86-87	24.908599891878513	32.0	18.0	34.0	2.0	35.0
88-89	24.51487403195446	31.0	15.5	34.0	2.0	35.0
90-91	24.1063418788388	31.0	9.0	34.0	2.0	35.0
92-93	23.75624613270284	31.0	4.5	34.0	2.0	35.0
94-95	23.326635337488845	31.0	2.0	34.0	2.0	35.0
96-97	22.732906058059935	31.0	2.0	34.0	2.0	35.0
98-99	22.0568670170845	30.0	2.0	34.0	2.0	35.0
100-101	19.855172408178156	27.0	2.0	32.5	2.0	34.5
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	95555.0
3	40893.0
4	21874.0
5	15385.0
6	12901.0
7	13030.0
8	14407.0
9	16836.0
10	18826.0
11	21023.0
12	23369.0
13	25131.0
14	26780.0
15	28615.0
16	30040.0
17	30808.0
18	29512.0
19	28973.0
20	28778.0
21	30195.0
22	32061.0
23	36621.0
24	42369.0
25	49600.0
26	58515.0
27	68830.0
28	80417.0
29	93917.0
30	110537.0
31	129051.0
32	154002.0
33	186156.0
34	229575.0
35	290753.0
36	367373.0
37	387455.0
38	190152.0
39	10305.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.060990148628655	19.779122296778354	13.55508108767701	24.604806466915978
2	16.505721971458534	20.867414398395113	37.44139620011594	25.185467430030418
3	19.753120196831585	24.23312973354843	29.121902236275414	26.891847833344567
4	14.245201039134297	16.25956600248579	35.39023557706088	34.10499738131904
5	14.577757310194833	37.010810835457356	34.00400854861749	14.40742330573032
6	34.16843800532053	34.997424495331494	16.592020499713108	14.242116999634865
7	29.631812246356866	31.45285743543718	20.48017882044773	18.435151497758223
8	26.917770810814336	34.88844435097192	19.57500436114521	18.618780477068526
9	26.597095667895935	15.701284538188498	19.38384693554089	38.317772858374674
10-11	26.00506819402592	25.44186126124511	27.22516784622946	21.327902698499514
12-13	26.295551432692942	22.563652555597923	27.47944079550636	23.66135521620277
14-15	23.331304838441223	24.314910219946338	24.767140143348783	27.586644798263656
16-17	23.637214767965766	27.503240847254258	25.469442183219808	23.39010220156017
18-19	23.650121770673515	26.592009986091497	26.876926473979207	22.880941769255777
20-21	24.18462843607871	25.848170006536886	26.814954395748764	23.152247161635646
22-23	23.954681020306094	25.553576238988164	26.680667646963443	23.811075093742303
24-25	23.62931445438955	25.918952269451808	26.74610625042971	23.705627025728937
26-27	23.58986959547435	26.122682546864763	26.694189142531542	23.593258715129338
28-29	23.632766557742368	26.20915746022851	26.624693344975093	23.533382637054025
30-31	23.895999911397244	26.04083805175573	26.429794369946467	23.63336766690055
32-33	23.613948489674836	26.09601721636793	26.367971196594254	23.92206309736298
34-35	23.922503474324202	26.071543798634632	26.49947239783174	23.50648032920942
36-37	24.00794432846026	25.868353082857443	26.448883875273914	23.67481871340838
38-39	23.735773182744705	25.976636371069862	26.51090045324489	23.776689992940547
40-41	23.92710811073172	25.8802593234683	26.59902783016932	23.593604735630656
42-43	23.862843917019877	25.80095440627116	26.49211886856182	23.844082808147142
44-45	23.63383016185067	25.799928659963744	26.571980956445117	23.99426022174047
46-47	23.75360449882501	25.842914788776326	26.449426741549352	23.95405397084931
48-49	23.650182188295165	25.893015776081423	26.461280407124683	23.99552162849873
50-51	23.68906288068396	25.932223761063046	26.426713900249236	23.95199945800376
52-53	23.82920466834624	25.862735222030448	26.460372648919606	23.84768746070371
54-55	23.448484540541667	25.80223788231738	26.56806148346179	24.181216093679158
56-57	23.473550354644807	25.89683033506472	26.443766769642334	24.18585254064814
58-59	23.35931070352546	25.76425023603701	26.515188277582784	24.36125078285474
60-61	23.4581978517965	25.816211789646225	26.608074362995183	24.11751599556209
62-63	23.287298013622504	25.823330876994415	26.57133900326924	24.31803210611384
64-65	23.117937854832412	25.733969672439088	26.558819922840055	24.58927254988844
66-67	23.124169229566306	25.759878023352794	26.428045506672227	24.687907240408673
68-69	23.213709185204284	25.696165996045867	26.43167857399545	24.6584462447544
70-71	23.060394530257526	25.437331839295368	26.56543365103356	24.93683997941355
72-73	23.120168806587404	25.495897044011333	26.41792792943955	24.96600621996171
74-75	22.981749793639114	25.61958379066817	26.31077256789538	25.08789384779734
76-77	22.911343478770416	25.42274558809943	26.58972548428156	25.07618544884859
78-79	22.886780658017475	25.39531501675583	26.561329723817266	25.15657460140943
80-81	22.882098743918363	25.454911447596384	26.43794035574702	25.22504945273823
82-83	22.848286887807507	25.421126747030492	26.510355605113663	25.22023076004834
84-85	22.702646187840124	25.372724761809224	26.479336388046754	25.44529266230391
86-87	22.67939768616855	25.320499791772473	26.500466654945193	25.499635867113778
88-89	22.573707074791326	25.215621332176454	26.48701881267862	25.723652780353607
90-91	22.477240364154174	25.21549859283884	26.533110164114927	25.774150878892062
92-93	22.57145410869806	25.01876254001899	26.486627983072292	25.923155368210658
94-95	22.336402791008197	24.954495493515058	26.657157047132866	26.05194466834388
96-97	22.392320048148697	24.741816284577567	26.690040801331904	26.175822865941832
98-99	22.281517407430385	24.779096395440046	26.561697253570127	26.37768894355944
100-101	22.330942440254677	24.68574441604308	26.69691827360009	26.286394870102153
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	51.0
1	41.5
2	53.5
3	86.0
4	77.0
5	86.5
6	168.5
7	330.5
8	499.5
9	526.5
10	460.5
11	428.0
12	432.5
13	470.0
14	529.0
15	611.5
16	692.0
17	766.0
18	846.0
19	946.0
20	1098.0
21	1310.5
22	1584.0
23	1965.0
24	2416.0
25	3079.5
26	4091.0
27	5658.5
28	7702.0
29	10663.5
30	15234.5
31	20678.0
32	27287.0
33	33237.0
34	38491.0
35	45545.5
36	54447.0
37	63371.5
38	71091.0
39	77411.0
40	83506.0
41	90486.0
42	98640.0
43	107033.5
44	113926.5
45	119783.5
46	125099.5
47	130044.5
48	134266.5
49	136806.5
50	136836.5
51	134422.5
52	131591.0
53	128861.0
54	124125.5
55	116385.0
56	106474.5
57	95621.0
58	85614.0
59	75618.0
60	63987.0
61	53046.5
62	43526.0
63	34039.0
64	25616.5
65	19548.5
66	14786.0
67	11219.0
68	8668.5
69	6638.5
70	5146.5
71	3852.5
72	2834.5
73	2380.0
74	1898.0
75	1372.5
76	984.5
77	643.5
78	391.5
79	189.0
80	103.0
81	70.5
82	41.5
83	24.5
84	12.5
85	6.5
86	3.0
87	2.5
88	3.0
89	2.0
90	1.5
91	0.5
92	0.5
93	0.5
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.18452299535598674
2	0.0
3	0.012407917619243019
4	0.012114817203040428
5	0.0059271417498746185
6	0.10616748409116074
7	0.14016713237066128
8	0.12261367411141724
9	0.14016713237066128
10-11	0.11243657632660504
12-13	0.11207834248457965
14-15	0.11351127785268122
16-17	0.06441695813874722
18-19	0.07661319212406616
20-21	0.03629560153975418
22-23	0.0814656323478646
24-25	0.054158443571656535
26-27	0.06436810806938012
28-29	0.03940572262279279
30-31	0.024034234128612462
32-33	0.032013078791905215
34-35	0.019491177677472302
36-37	0.024653001673929042
38-39	0.03181767851443682
40-41	0.025597436348359617
42-43	0.014296786968104162
44-45	0.026541871022790185
46-47	0.03316919710025988
48-49	0.010014264220255193
50-51	0.015892555900762714
52-53	0.006399359087089904
54-55	0.012896418312914005
56-57	0.004998990431899747
58-59	0.0041359725397476736
60-61	0.022992099315447694
62-63	0.010828432043040168
64-65	0.007962561306837056
66-67	0.03842872123545082
68-69	0.08765330780103041
70-71	0.02084269626329536
72-73	0.042613543844565595
74-75	0.08377786896457394
76-77	0.24004924086992205
78-79	0.07926737922634516
80-81	0.016836990575193285
82-83	0.012196233985318926
84-85	0.012766151461268409
86-87	0.2561209136916974
88-89	0.6103653333854401
90-91	0.26284593990790134
92-93	0.34737284326943746
94-95	0.001465502081012955
96-97	0.004298806104304668
98-99	0.0034683549250639936
100-101	0.00486872358025415
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3070620.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.84081054926635
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.80500584416006	40.475263430870775
2	12.77224202720156	14.008802106062767
3	5.320931134621267	8.754125288983731
4	2.7355246697975697	6.000735606768516
5	1.5355337103557198	4.210495665081503
6	0.9246500145406135	3.042513376307889
7	0.6445968204366197	2.474514847715688
8	0.4374553015326049	1.9192322652097416
9	0.3204319850886532	1.5815474809354955
>10	1.4220833120614558	13.09076566839161
>50	0.06110470916775325	2.258239596789716
>100	0.019367456353254216	1.699143442076139
>500	8.345669757586968E-4	0.3140688146391604
>1k	2.3844770707136167E-4	0.17055241016729733
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	3.2566712911399E-5	0.0
10-11	0.0	0.0	0.0	3.2566712911399E-5	0.0
12-13	0.0	0.0	0.0	3.2566712911399E-5	0.0
14-15	0.0	0.0	0.0	3.2566712911399E-5	0.0
16-17	0.0	0.0	0.0	4.88500693670985E-5	0.0
18-19	0.0	0.0	0.0	8.14167822784975E-5	0.0
20-21	0.0	0.0	0.0	2.116836339240935E-4	0.0
22-23	0.0	0.0	0.0	3.7451719848108854E-4	0.0
24-25	0.0	0.0	0.0	7.16467684050778E-4	0.0
26-27	0.0	0.0	0.0	0.0012538184470888615	0.0
28-29	0.0	0.0	0.0	0.004998990431899747	0.0
30-31	0.0	0.0	0.0	0.02188483107646013	0.0
32-33	0.0	0.0	0.0	0.05720343122887235	0.0
34-35	0.0	0.0	0.0	0.10667226814128743	0.0
36-37	0.0	0.0	0.0	0.1918342224045958	0.0
38-39	0.0	0.0	0.0	0.3355511264825996	0.0
40-41	0.0	0.0	0.0	0.49726113944415135	0.0
42-43	0.0	0.0	0.0	0.6708905693312751	0.0
44-45	0.0	0.0	0.0	0.8839908552670145	0.0
46-47	0.0	0.0	0.0	1.1412516039106109	0.0
48-49	0.0	0.0	0.0	1.412402055610919	0.0
50-51	0.0	0.0	0.0	1.7100455282646503	0.0
52-53	0.0	0.0	0.0	2.0118086901016734	0.0
54-55	0.0	0.0	0.0	2.3537103256019956	0.0
56-57	3.2566712911399E-5	0.0	0.0	2.751919807726127	0.0
58-59	3.2566712911399E-5	0.0	0.0	3.1863597579641896	0.0
60-61	3.2566712911399E-5	0.0	0.0	3.6522917195875753	0.0
62-63	3.2566712911399E-5	0.0	0.0	4.128628745986152	0.0
64-65	3.2566712911399E-5	0.0	0.0	4.63767903550423	0.0
66-67	3.2566712911399E-5	0.0	0.0	5.212351251538777	0.0
68-69	3.2566712911399E-5	0.0	0.0	5.84977952335359	0.0
70-71	3.2566712911399E-5	0.0	0.0	6.535439096990184	0.0
72-73	3.2566712911399E-5	0.0	0.0	7.224094808214628	0.0
74-75	3.2566712911399E-5	0.0	0.0	7.9351075678527465	0.0
76-77	3.2566712911399E-5	0.0	3.2566712911399E-5	8.71096065289746	0.0
78-79	3.2566712911399E-5	0.0	3.2566712911399E-5	9.564029414255101	0.0
80-81	3.2566712911399E-5	0.0	3.2566712911399E-5	10.447010701421863	0.0
82-83	3.2566712911399E-5	0.0	3.2566712911399E-5	11.348017664185083	0.0
84-85	3.2566712911399E-5	0.0	3.2566712911399E-5	12.24327660211944	0.0
86-87	3.2566712911399E-5	0.0	3.2566712911399E-5	13.177583028834567	0.0
88-89	3.2566712911399E-5	0.0	3.2566712911399E-5	14.1779021826211	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	4685	0.0	44.050705	1
GGTATCA	2660	0.0	41.295532	1
TCAACGC	5935	0.0	34.23463	4
CAACGCA	6010	0.0	33.72457	5
ATCAACG	6075	0.0	33.36754	3
TATCAAC	6485	0.0	31.54922	2
AACGCAG	6550	0.0	31.08916	6
GTACATG	9610	0.0	26.571964	1
ACGCAGA	7780	0.0	25.8694	7
TACATGG	9885	0.0	25.259829	2
CGCAGAG	8140	0.0	24.663767	8
ACATGGG	9975	0.0	23.605398	3
GCAGAGT	9195	0.0	20.595135	9
GAGTACA	7320	0.0	19.878473	1
ATACCGT	415	0.0	19.444366	6
CATGGGG	6985	0.0	19.301653	4
AGAGTAC	7955	0.0	18.70244	10-11
AGTACAT	7445	0.0	17.980665	2
TAAGGTG	1235	0.0	16.911367	5
ATGGGGG	3610	0.0	16.830452	5
>>END_MODULE