FastQCFastQC Report
Mon 15 Aug 2016
SRR2920873_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920873_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3914014
Sequences flagged as poor quality0
Sequence length98
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA63350.042.5071871
GGTATCA33450.041.2836651
TCAACGC77500.033.9756134
CAACGCA78450.033.505715
ATCAACG80400.032.9212953
TATCAAC81550.032.290352
AACGCAG83300.031.6055246
GTACATG126750.027.201131
ACGCAGA100700.025.6826747
TACATGG131900.025.1465472
ACATGGG132350.024.4355623
CGCAGAG105700.023.9884348
CATGGGG93100.022.2220424
GCAGAGT119300.020.5240789
GAGTACA96550.017.783191
TATAGCG2102.6417038E-517.4754285
AGAGTAC100850.017.36835510-11
AGTACAT94500.016.6512642
CAGAGTA110250.016.49057410-11
TAATACT22850.016.4620824
Produced by FastQC (version 0.11.5)