##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920873_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3914014
Sequences flagged as poor quality	0
Sequence length	98
%GC	49
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.629030453135833	31.0	30.0	33.0	22.0	34.0
2	28.660144547260177	31.0	28.0	34.0	18.0	34.0
3	28.74216367136142	31.0	28.0	34.0	22.0	34.0
4	32.21375626147479	35.0	32.0	37.0	22.0	37.0
5	32.179918109643964	35.0	32.0	37.0	22.0	37.0
6	32.302693347545514	35.0	32.0	37.0	23.0	37.0
7	32.13067556733318	35.0	32.0	37.0	22.0	37.0
8	32.069808130476794	35.0	32.0	37.0	22.0	37.0
9	33.333827370060504	37.0	33.0	39.0	22.0	39.0
10-11	33.13419752714221	37.0	32.0	39.0	19.0	39.0
12-13	32.9685578539065	37.0	32.0	39.0	18.0	39.0
14-15	33.74637431036271	38.0	32.0	40.0	17.5	41.0
16-17	33.634433474177655	38.0	32.0	40.0	17.0	41.0
18-19	33.506754446969275	38.0	32.0	40.0	17.5	41.0
20-21	33.40413894789339	38.0	32.0	40.0	17.5	41.0
22-23	33.18416260136014	38.0	32.0	40.0	16.0	41.0
24-25	32.99284736845601	38.0	32.0	40.0	14.5	41.0
26-27	33.03922954797811	38.0	32.0	40.0	12.0	41.0
28-29	32.87449495581774	38.0	31.5	40.0	10.0	41.0
30-31	32.62637384536693	38.0	31.0	40.0	9.5	41.0
32-33	32.39047177654449	37.0	31.0	40.0	9.0	41.0
34-35	32.0678852962713	37.0	30.0	40.0	7.5	41.0
36-37	31.76792354345181	37.0	30.0	40.0	4.5	41.0
38-39	31.56672740567612	37.0	30.0	40.0	2.0	41.0
40-41	31.366817798812164	37.0	30.0	40.0	2.0	41.0
42-43	31.13107477387664	36.5	30.0	40.0	2.0	41.0
44-45	30.846938973647003	36.0	29.0	39.0	2.0	41.0
46-47	30.60040319222159	36.0	28.5	39.0	2.0	40.0
48-49	30.300205492366658	35.5	28.0	39.0	2.0	40.0
50-51	29.145542274503875	34.0	26.0	38.0	2.0	39.5
52-53	29.588304998398065	34.5	27.5	38.5	2.0	39.5
54-55	30.236469261479392	35.5	28.0	39.0	2.0	40.0
56-57	30.17992347497991	35.0	28.0	39.0	2.0	41.0
58-59	29.927137588163966	35.0	28.0	39.0	2.0	40.0
60-61	29.59541611246153	35.0	28.0	39.0	2.0	40.0
62-63	29.188808854541655	34.5	26.5	38.5	2.0	40.0
64-65	28.755585825702205	34.0	26.0	38.0	2.0	40.0
66-67	28.364283827293413	34.0	26.0	37.5	2.0	40.0
68-69	27.915388141176805	34.0	25.5	37.0	2.0	39.5
70-71	27.575311304456243	34.0	25.0	36.0	2.0	39.0
72-73	27.111632329368263	33.0	24.0	36.0	2.0	39.0
74-75	26.665965425775177	33.0	23.0	35.0	2.0	38.0
76-77	26.247912245587266	33.0	21.5	35.0	2.0	37.0
78-79	25.795832232587824	32.0	20.0	35.0	2.0	37.0
80-81	25.349948160635094	32.0	18.5	35.0	2.0	36.0
82-83	24.88307221180098	32.0	17.0	35.0	2.0	36.0
84-85	24.483986771636484	31.0	10.0	34.5	2.0	35.0
86-87	24.03040855244769	31.0	4.5	34.0	2.0	35.0
88-89	23.500999868677017	31.0	2.0	34.0	2.0	35.0
90-91	23.0056761421906	31.0	2.0	34.0	2.0	35.0
92-93	22.47380962357314	30.0	2.0	34.0	2.0	35.0
94-95	21.8369308336659	30.0	2.0	34.0	2.0	35.0
96-97	21.192553859030653	29.0	2.0	34.0	2.0	35.0
98	20.791392161601873	29.0	2.0	34.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	196178.0
3	34279.0
4	24990.0
5	20738.0
6	19157.0
7	19503.0
8	22576.0
9	25615.0
10	28208.0
11	30027.0
12	31132.0
13	31156.0
14	29095.0
15	30236.0
16	32555.0
17	34734.0
18	36236.0
19	36003.0
20	37278.0
21	39725.0
22	43485.0
23	49080.0
24	55541.0
25	63733.0
26	73473.0
27	85517.0
28	98805.0
29	113385.0
30	132142.0
31	154861.0
32	183565.0
33	223444.0
34	277988.0
35	352859.0
36	448382.0
37	488500.0
38	283047.0
39	26784.0
40	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.99154255750189	17.27982697375372	12.551942237052522	25.17668823169187
2	16.21895266663225	19.099914186517317	38.88669400363596	25.794439143214476
3	19.88621408862156	22.956757367285118	28.364015767709926	28.793012776383392
4	13.583404775624357	14.96690230910854	35.507350996508166	35.942341918758935
5	14.873356584598968	36.40043729006908	33.69231563388661	15.033890491445348
6	34.77898210175916	33.97461157612771	16.77131456673214	14.475091755380998
7	30.27055577119293	30.835477427536684	20.15429812419333	18.739668677077052
8	27.343970777689368	34.045443186818076	19.43694518925974	19.17364084623282
9	27.226346150013768	15.562591242647574	18.87808781470889	38.33297479262976
10-11	26.28114674120909	24.84554854569977	26.983482170345376	21.889822542745772
12-13	26.714750988740153	22.146131438372596	26.987872970109244	24.151244602778004
14-15	23.83189646637399	23.62455838457701	24.37915147241337	28.16439367663563
16-17	24.132041612281068	26.784887768181097	25.024257137538957	24.05881348199887
18-19	24.236971820006676	25.889298615715145	26.403568542317018	23.470161021961164
20-21	24.88664673116748	25.182508766365387	26.17006640180603	23.760778100661103
22-23	24.585926977074497	24.93901458202964	26.11936596135242	24.355692479543446
24-25	24.316847933418423	25.23228071431265	26.065777557350362	24.385093794918568
26-27	24.088934262034005	25.356910835378066	26.09508520376113	24.459069698826802
28-29	24.283171484635023	25.503683135798504	25.976294286844908	24.236851092721565
30-31	24.341748655851962	25.395416035657004	25.804728236509217	24.458107071981814
32-33	24.13780855249759	25.40038325582441	25.74133477184166	24.72047341983634
34-35	24.470513809838543	25.405853008715006	25.779988688265593	24.343644493180854
36-37	24.675372861747697	25.073215309821666	25.81350165932449	24.43791016910615
38-39	24.448817093936807	25.32658519986905	25.71839119180194	24.506206514392208
40-41	24.474481315525797	25.23200700028479	25.97327175656402	24.32023992762539
42-43	24.511372080973892	25.081011990197872	25.785371494221437	24.6222444346068
44-45	24.31528976505716	25.133563991830083	25.920776526902735	24.63036971621002
46-47	24.442850486880225	25.257534367091615	25.747242418502438	24.55237272752572
48-49	24.246134094154872	25.30942570737463	25.66918650972008	24.775253688750418
50-51	24.162392871957987	25.47242769277918	25.833407599799706	24.531771835463125
52-53	24.342368033203034	25.228538359122233	25.880694951120653	24.548398656554085
54-55	24.05153089314407	25.11624230851791	26.069968766395142	24.76225803194288
56-57	23.944350651133092	25.30368381532207	26.02365900292223	24.72830653062261
58-59	23.9928343535496	25.086380334076207	25.965041566828518	24.95574374554568
60-61	23.857878568267672	24.962026128281266	26.30263655778814	24.877458745662924
62-63	23.894069518689832	25.035777359725113	26.239068812696946	24.83108430888811
64-65	23.804037244368374	24.91836595915026	26.109498032443195	25.168098764038167
66-67	23.72201507501253	25.02947436368805	26.100266093798226	25.1482444675012
68-69	23.941071116166274	24.80156867332286	26.104217553893644	25.153142656617224
70-71	23.658080823297357	24.745793351286657	26.150716904352713	25.44540892106328
72-73	23.626997389542687	24.668590881192145	26.143119822093052	25.56129190717212
74-75	23.622077722001617	24.860892120676453	26.16312438334843	25.3539057739735
76-77	23.585063327674415	24.617651363664212	26.33333393143729	25.46395137722408
78-79	23.529035566343527	24.602919801392297	26.277392282516015	25.590652349748165
80-81	23.482021813990407	24.548917401764232	26.3312897244948	25.637771059750563
82-83	23.46488344042689	24.440979338827084	26.401477134789193	25.692660085956838
84-85	23.356299350114405	24.530005472005673	26.337843860567293	25.775851317312625
86-87	23.364401418595328	24.431718565287067	26.439011474053427	25.76486854206418
88-89	23.34228923656855	24.14823077638129	26.43244126895597	26.077038718094194
90-91	23.165805988066936	24.19158276198674	26.49229619479004	26.150315055156288
92-93	23.359905806448968	24.02099615883908	26.568982471755266	26.050115562956687
94-95	23.174766559397327	23.883167216472774	26.712321831094695	26.229744393035205
96-97	23.381173926502854	23.567759290011672	26.659761794736873	26.391304988748605
98	23.29948200130128	23.54861153481533	26.605639587139194	26.546266876744202
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	58.0
1	59.0
2	106.5
3	164.0
4	152.0
5	170.0
6	292.5
7	543.5
8	863.0
9	1037.5
10	1048.0
11	1104.0
12	1270.0
13	1452.5
14	1643.0
15	1821.5
16	1973.5
17	2066.0
18	2065.5
19	2098.5
20	2144.0
21	2196.0
22	2334.5
23	2588.5
24	2986.5
25	3510.0
26	4374.5
27	5727.0
28	7350.0
29	9912.5
30	14189.5
31	20302.0
32	26837.5
33	33695.5
34	41608.5
35	50709.5
36	61194.0
37	72410.0
38	83640.0
39	94771.0
40	104614.5
41	114279.0
42	125311.5
43	135554.5
44	146390.0
45	158054.0
46	167798.5
47	174859.0
48	179319.0
49	181327.0
50	181753.0
51	181826.5
52	180657.5
53	179306.0
54	175087.5
55	167154.5
56	156140.5
57	142753.0
58	131266.0
59	116441.0
60	97658.0
61	80585.5
62	65574.0
63	50530.0
64	37008.0
65	27338.0
66	20337.0
67	15099.0
68	10904.0
69	7835.5
70	5690.5
71	4126.5
72	3153.5
73	2385.0
74	1826.5
75	1307.5
76	825.5
77	490.5
78	270.0
79	155.5
80	92.0
81	57.0
82	42.0
83	29.5
84	20.5
85	18.0
86	15.5
87	9.0
88	7.5
89	9.5
90	6.0
91	5.5
92	6.5
93	4.5
94	3.5
95	3.5
96	2.5
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3702081801444757
2	0.02238111565262669
3	0.017884453147076124
4	0.015303982050140854
5	0.021001457838423675
6	0.022789903153131286
7	0.014077619548627063
8	0.007460371884208897
9	0.0
10-11	2.5549218781537316E-5
12-13	0.004982097662399776
14-15	0.0029764839880490977
16-17	0.0055569550849843665
18-19	0.03525792191852149
20-21	0.074105509075849
22-23	0.09333129620895582
24-25	0.06186743327949261
26-27	0.07008150711775686
28-29	0.05441983600467449
30-31	0.05016589107754852
32-33	0.017910002365857658
34-35	0.32589306016789926
36-37	0.49840138538083917
38-39	0.4554275993902935
40-41	0.4642931783074869
42-43	0.41615845012307057
44-45	0.3914395809519332
46-47	0.3899449516532133
48-49	0.38056838836038914
50-51	0.3772725391375708
52-53	0.43486047827115587
54-55	0.436623374367082
56-57	0.4078421794096802
58-59	0.3937773344704439
60-61	0.4072289981589233
62-63	0.3896128118090533
64-65	0.4355886310064297
66-67	0.4555808947029827
68-69	0.40081614424475737
70-71	0.3598990703661254
72-73	0.3461408160522676
74-75	0.32971266837573904
76-77	0.32695335274733306
78-79	0.32589306016789926
80-81	0.34230843323503696
82-83	0.3377351230731418
84-85	0.3385399234647602
86-87	0.32421958633770853
88-89	0.36666961334323284
90-91	0.3450805234728338
92-93	0.3349502582259542
94-95	0.3422701094068647
96-97	0.3420146172190493
98	0.33806726291730177
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
98	3914014.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.283016086135056
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.57937893781465	35.04351320776197
2	12.115911730925996	11.699855220049846
3	5.3233915527710485	7.710881999257199
4	2.8815666315015434	5.565229120882361
5	1.7521137714730857	4.229866870638689
6	1.1768548707574176	3.4093261593496065
7	0.810524509984461	2.7394197567650527
8	0.5941762509241197	2.2950897185094954
9	0.4255831038265994	1.8493592263242284
>10	2.2029488217803435	18.380586695141297
>50	0.10045309846390253	3.2739983060408746
>100	0.03475179103626252	2.7885234004368273
>500	0.001758696600864074	0.5670017773997234
>1k	5.862321396514139E-4	0.4473485414428535
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	2.5549218781537316E-5	0.0
5	0.0	0.0	0.0	2.5549218781537316E-5	0.0
6	0.0	0.0	0.0	2.5549218781537316E-5	0.0
7	0.0	0.0	0.0	2.5549218781537316E-5	0.0
8	0.0	0.0	0.0	2.5549218781537316E-5	0.0
9	0.0	0.0	0.0	2.5549218781537316E-5	0.0
10-11	0.0	0.0	0.0	5.109843756307463E-5	0.0
12-13	0.0	0.0	0.0	5.109843756307463E-5	0.0
14-15	0.0	0.0	0.0	5.109843756307463E-5	1.2774609390768658E-5
16-17	0.0	0.0	0.0	6.387304695384328E-5	5.109843756307463E-5
18-19	0.0	0.0	1.2774609390768658E-5	1.0219687512614926E-4	5.109843756307463E-5
20-21	0.0	0.0	2.5549218781537316E-5	1.2774609390768657E-4	7.664765634461195E-5
22-23	0.0	0.0	2.5549218781537316E-5	3.065906253784478E-4	1.0219687512614926E-4
24-25	0.0	0.0	2.5549218781537316E-5	7.281527352738135E-4	1.0219687512614926E-4
26-27	0.0	0.0	2.5549218781537316E-5	0.001571276955064545	1.0219687512614926E-4
28-29	0.0	0.0	2.5549218781537316E-5	0.007268752743347366	1.0219687512614926E-4
30-31	0.0	0.0	2.5549218781537316E-5	0.030020332068306347	1.1497148451691793E-4
32-33	0.0	0.0	2.5549218781537316E-5	0.07443764892000898	1.277460939076866E-4
34-35	0.0	0.0	2.5549218781537316E-5	0.13447831305662167	1.277460939076866E-4
36-37	0.0	0.0	2.5549218781537316E-5	0.2322934971617373	1.277460939076866E-4
38-39	0.0	0.0	2.5549218781537316E-5	0.39527196376916385	1.277460939076866E-4
40-41	0.0	0.0	2.5549218781537316E-5	0.5795968026685647	1.277460939076866E-4
42-43	0.0	0.0	2.5549218781537316E-5	0.7741668782993623	1.277460939076866E-4
44-45	0.0	0.0	2.5549218781537316E-5	1.0053106606159303	1.277460939076866E-4
46-47	0.0	0.0	2.5549218781537316E-5	1.2863265179940595	1.277460939076866E-4
48-49	0.0	0.0	2.5549218781537316E-5	1.5836811007829814	1.277460939076866E-4
50-51	0.0	0.0	2.5549218781537316E-5	1.9091270496222037	1.277460939076866E-4
52-53	0.0	0.0	2.5549218781537316E-5	2.2330911437720973	1.7884453147076122E-4
54-55	0.0	0.0	2.5549218781537316E-5	2.5932584809354284	1.7884453147076122E-4
56-57	0.0	0.0	2.5549218781537316E-5	3.019253380289391	1.7884453147076122E-4
58-59	0.0	0.0	2.5549218781537316E-5	3.47344184256878	1.7884453147076122E-4
60-61	0.0	0.0	2.5549218781537316E-5	3.961316949811626	1.7884453147076122E-4
62-63	0.0	0.0	2.5549218781537316E-5	4.45348432580977	2.2994296903383586E-4
64-65	0.0	0.0	2.5549218781537316E-5	4.983873333105094	2.554921878153732E-4
66-67	0.0	0.0	2.5549218781537316E-5	5.5858257022075035	2.554921878153732E-4
68-69	0.0	0.0	2.5549218781537316E-5	6.24505941981812	2.554921878153732E-4
70-71	2.5549218781537316E-5	0.0	2.5549218781537316E-5	6.937698740985597	2.554921878153732E-4
72-73	5.109843756307463E-5	0.0	2.5549218781537316E-5	7.645859212562858	2.554921878153732E-4
74-75	5.109843756307463E-5	0.0	2.5549218781537316E-5	8.368135627516917	2.554921878153732E-4
76-77	5.109843756307463E-5	0.0	2.5549218781537316E-5	9.142430251910188	2.554921878153732E-4
78-79	5.109843756307463E-5	0.0	2.5549218781537316E-5	9.991980100224476	2.554921878153732E-4
80-81	5.109843756307463E-5	2.5549218781537316E-5	2.5549218781537316E-5	10.872431728655034	2.554921878153732E-4
82-83	5.109843756307463E-5	5.109843756307463E-5	2.5549218781537316E-5	11.777487254772211	2.554921878153732E-4
84-85	5.109843756307463E-5	5.109843756307463E-5	2.5549218781537316E-5	12.691114543790594	2.554921878153732E-4
86	5.109843756307463E-5	5.109843756307463E-5	2.5549218781537316E-5	13.3911886876235	2.554921878153732E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	6335	0.0	42.507187	1
GGTATCA	3345	0.0	41.283665	1
TCAACGC	7750	0.0	33.975613	4
CAACGCA	7845	0.0	33.50571	5
ATCAACG	8040	0.0	32.921295	3
TATCAAC	8155	0.0	32.29035	2
AACGCAG	8330	0.0	31.605524	6
GTACATG	12675	0.0	27.20113	1
ACGCAGA	10070	0.0	25.682674	7
TACATGG	13190	0.0	25.146547	2
ACATGGG	13235	0.0	24.435562	3
CGCAGAG	10570	0.0	23.988434	8
CATGGGG	9310	0.0	22.222042	4
GCAGAGT	11930	0.0	20.524078	9
GAGTACA	9655	0.0	17.78319	1
TATAGCG	210	2.6417038E-5	17.475428	5
AGAGTAC	10085	0.0	17.368355	10-11
AGTACAT	9450	0.0	16.651264	2
CAGAGTA	11025	0.0	16.490574	10-11
TAATACT	2285	0.0	16.462082	4
>>END_MODULE