FastQCFastQC Report
Mon 15 Aug 2016
SRR2920872_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920872_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4419357
Sequences flagged as poor quality0
Sequence length98
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA60100.042.269961
GGTATCA35650.037.43741
TCAACGC72750.034.1693274
ATCAACG74050.033.63143
CAACGCA74950.032.8603975
TATCAAC80250.030.9194812
AACGCAG82650.029.738796
GTACATG124950.025.0092541
ACGCAGA96550.024.836587
TACATGG128700.023.4848022
CGCAGAG100800.023.333488
ACATGGG131400.021.8847893
GCAGAGT117350.019.2218369
CATGGGG94550.018.7238854
TAAGGCG11800.018.6564675
GAGTACA94350.018.0479161
AGAGTAC97500.017.77531410-11
GTATATA14950.017.5469191
AGTACAT92850.016.745492
GTATTAA24950.016.2322711
Produced by FastQC (version 0.11.5)