##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920872_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4419357
Sequences flagged as poor quality	0
Sequence length	98
%GC	48
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.377001224386262	31.0	28.0	33.0	16.0	34.0
2	28.410946207785432	31.0	28.0	33.0	16.0	34.0
3	28.481434968933264	31.0	28.0	34.0	16.0	34.0
4	31.930147756789054	35.0	32.0	37.0	20.0	37.0
5	31.88844576258492	35.0	32.0	37.0	20.0	37.0
6	32.004193596489266	35.0	32.0	37.0	22.0	37.0
7	31.835290744784817	35.0	32.0	37.0	19.0	37.0
8	31.76899829545339	35.0	32.0	37.0	18.0	37.0
9	33.012948716295156	37.0	32.0	39.0	18.0	39.0
10-11	32.812123007939846	37.0	32.0	39.0	17.0	39.0
12-13	32.63410287967231	37.0	32.0	39.0	17.0	39.0
14-15	33.37906374162576	38.0	32.0	40.0	17.0	41.0
16-17	33.26466508589372	38.0	32.0	40.0	16.5	41.0
18-19	33.14279339732002	38.0	32.0	40.0	14.5	41.0
20-21	33.04452265340863	38.0	32.0	40.0	11.0	41.0
22-23	32.819198697910124	38.0	32.0	40.0	10.0	41.0
24-25	32.62407472399265	38.0	31.0	40.0	10.0	41.0
26-27	32.664725547178016	38.0	31.0	40.0	10.0	41.0
28-29	32.499770441718105	38.0	31.0	40.0	9.0	41.0
30-31	32.25583076904627	37.5	30.5	40.0	8.0	41.0
32-33	32.01343294963498	37.0	30.0	40.0	7.5	41.0
34-35	31.686008620711114	37.0	30.0	40.0	2.0	41.0
36-37	31.38332872859106	37.0	30.0	40.0	2.0	41.0
38-39	31.179214193377	37.0	30.0	40.0	2.0	41.0
40-41	30.984873704477824	36.5	29.5	40.0	2.0	41.0
42-43	30.75907501928448	36.0	29.0	39.5	2.0	41.0
44-45	30.48010513746683	36.0	28.0	39.0	2.0	40.5
46-47	30.24045477656591	36.0	28.0	39.0	2.0	40.0
48-49	29.94060923342468	35.0	27.0	39.0	2.0	40.0
50-51	28.826781927778182	34.0	25.5	38.0	2.0	39.5
52-53	29.279271735684624	34.5	26.5	38.5	2.0	39.5
54-55	29.918048824749846	35.0	28.0	39.0	2.0	40.0
56-57	29.86774037490069	35.0	28.0	39.0	2.0	41.0
58-59	29.62480005575472	35.0	27.5	39.0	2.0	40.0
60-61	29.30089942948714	35.0	26.5	39.0	2.0	40.0
62-63	28.910182182611635	34.5	26.0	38.5	2.0	40.0
64-65	28.487877648264217	34.0	26.0	38.0	2.0	40.0
66-67	28.09588747865357	34.0	25.5	38.0	2.0	40.0
68-69	27.64559391784823	34.0	24.0	37.0	2.0	39.5
70-71	27.310259501551926	33.5	23.5	36.5	2.0	39.0
72-73	26.84465240079043	33.0	22.5	36.0	2.0	39.0
74-75	26.395684372183553	33.0	21.5	35.5	2.0	38.5
76-77	25.965392816194754	32.5	20.0	35.0	2.0	37.5
78-79	25.495248403783627	32.0	18.5	35.0	2.0	37.0
80-81	25.042310114344687	32.0	16.5	35.0	2.0	36.0
82-83	24.561339013797706	31.5	9.5	35.0	2.0	36.0
84-85	24.152223049642743	31.0	6.0	34.0	2.0	35.5
86-87	23.69198878931935	31.0	2.0	34.0	2.0	35.0
88-89	23.149858339120374	31.0	2.0	34.0	2.0	35.0
90-91	22.641163974759223	30.0	2.0	34.0	2.0	35.0
92-93	22.094653815023314	30.0	2.0	34.0	2.0	35.0
94-95	21.452173585433357	29.5	2.0	34.0	2.0	35.0
96-97	20.808016301919032	29.0	2.0	34.0	2.0	35.0
98	20.408988683195314	29.0	2.0	34.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	248409.0
3	41924.0
4	30503.0
5	24377.0
6	22758.0
7	23021.0
8	26228.0
9	29949.0
10	32831.0
11	34951.0
12	36413.0
13	35728.0
14	33487.0
15	35455.0
16	37731.0
17	40278.0
18	41799.0
19	42209.0
20	43196.0
21	46062.0
22	49760.0
23	55826.0
24	63572.0
25	73060.0
26	84172.0
27	96111.0
28	110243.0
29	127674.0
30	148695.0
31	173768.0
32	205666.0
33	247225.0
34	306171.0
35	384769.0
36	488312.0
37	538447.0
38	326303.0
39	32274.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.207504720599985	17.829741347005093	13.079593608237214	24.883160324157707
2	15.889397554857501	19.158814307096083	39.61359895217878	25.338189185867634
3	19.670894939538393	23.163322046924932	28.65446412870715	28.511318884829524
4	13.342586492635455	15.33121610547606	36.34513635434727	34.98106104754122
5	14.636683416403033	36.54739363630726	34.12123181855423	14.694691128735476
6	33.99124966078912	34.619833724996	17.12309343117997	14.265823183034906
7	29.793842780084255	31.061247444109885	20.55531981582881	18.58958995997705
8	26.81692906124738	34.47826546436837	19.69282058465515	19.011984889729103
9	26.71001686444431	16.035590697922796	19.296856986208628	37.95753545142427
10-11	25.77176986234417	25.212412218525103	27.470897902521973	21.54492001660876
12-13	26.389758059488493	22.406298443571444	27.36702198976788	23.83692150717218
14-15	23.458755436741683	24.099284559755453	24.80144196837599	27.640518035126878
16-17	23.710329605377346	27.072572832725335	25.575740761819116	23.641356800078206
18-19	23.670607297310177	26.381460727376894	26.900284837730094	23.04764713758284
20-21	24.35966458979913	25.63660467993222	26.694807355618757	23.308923374649897
22-23	24.0593989552671	25.427367825501722	26.53242648032233	23.980806738908843
24-25	23.840169378182505	25.60652923821592	26.60384042070128	23.949460962900297
26-27	23.713992217997514	25.845613738731725	26.461152088252938	23.97924195501782
28-29	23.815478157335377	25.979600585518416	26.403027163259274	23.801894093886933
30-31	23.97408970125052	25.79659453325523	26.259618024014287	23.96969774147997
32-33	23.744133969604768	25.821917552448433	26.16588750913902	24.268060968807788
34-35	24.07258308473456	25.820960528671144	26.213311651199454	23.893144735394838
36-37	24.27551582209178	25.53076307489526	26.169711830052115	24.024009272960846
38-39	23.945534872429473	25.73559563675246	26.224231080819365	24.0946384099987
40-41	24.1666451650376	25.58669610287046	26.32459791290601	23.922060819185926
42-43	24.075449610393328	25.476661205000617	26.184481309491055	24.263407875115004
44-45	23.991830718085605	25.613590602940572	26.27587670931184	24.11870196966198
46-47	24.07023440507235	25.58806281801972	26.14662013025697	24.195082646650967
48-49	23.901046047390924	25.62151723548934	26.136432036447776	24.34100468067196
50-51	23.847941117249118	25.756740357172745	26.228892146222034	24.166426379356103
52-53	23.991847968128212	25.594516525648103	26.297041223782024	24.116594282441657
54-55	23.685062780448426	25.47883046188083	26.517080536424658	24.31902622124608
56-57	23.657674681526363	25.649997256520763	26.413956822976065	24.27837123897681
58-59	23.643158762294544	25.415645150151565	26.46805326364304	24.473142823910845
60-61	23.568089816262816	25.403916669942184	26.64106234895229	24.386931164842714
62-63	23.49393211381966	25.469538811573873	26.63568513140726	24.400843943199206
64-65	23.412484582733253	25.340702561465093	26.54316414244316	24.703648713358493
66-67	23.417097203922076	25.418517218213143	26.444407645255108	24.719977932609673
68-69	23.597566752217162	25.21687697608804	26.496510771206232	24.689045500488564
70-71	23.33520308577281	25.05792636884614	26.589719162426604	25.017151382954445
72-73	23.316633131159048	25.016652329447165	26.604989942753683	25.061724596640104
74-75	23.285293833196587	25.152663766482668	26.58899040995937	24.973051990361377
76-77	23.25287322268493	24.93502905374719	26.698617786039875	25.113479937528
78-79	23.192576715480147	24.979258877024694	26.653486636973195	25.174677770521964
80-81	23.194343136648307	24.951810285959507	26.650572208507434	25.203274368884752
82-83	23.19226874046327	24.852989207156057	26.753392978106778	25.2013490742739
84-85	23.113060877851392	24.806571335515166	26.751920777431394	25.328447009202044
86-87	23.094267954194123	24.738179885245803	26.849256199885563	25.318295960674515
88-89	23.043744225048492	24.5844731830471	26.77162198054563	25.60016061135878
90-91	22.971002119414738	24.558065469797548	26.81983793993069	25.65109447085703
92-93	23.068069420871783	24.325855304958502	26.953925002818124	25.652150271351594
94-95	22.87797952266786	24.232962860114704	27.052427610362596	25.83663000685484
96-97	23.074062162004758	24.0238859148871	26.99478711784506	25.907264805263086
98	22.98248673154677	23.929237640631072	26.924516032941366	26.16375959488079
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	53.0
1	42.5
2	63.5
3	117.0
4	147.0
5	175.0
6	267.5
7	514.5
8	805.5
9	1005.5
10	1114.0
11	1242.5
12	1438.5
13	1620.5
14	1820.5
15	2073.5
16	2206.5
17	2235.0
18	2235.0
19	2207.5
20	2224.5
21	2310.5
22	2503.5
23	2850.5
24	3294.5
25	4038.0
26	5360.5
27	7273.0
28	9782.0
29	13194.5
30	18377.5
31	26111.0
32	35190.0
33	44631.5
34	55817.0
35	69098.5
36	83449.0
37	97424.5
38	110317.5
39	122334.5
40	133525.0
41	143459.0
42	154393.5
43	165877.5
44	177097.0
45	188108.5
46	196048.0
47	201218.5
48	203997.0
49	202568.0
50	200461.0
51	199116.5
52	195377.0
53	191892.5
54	185367.5
55	173978.0
56	159512.5
57	143949.5
58	130847.5
59	114639.0
60	95558.0
61	78524.5
62	63270.5
63	48254.5
64	35255.0
65	26385.5
66	19812.5
67	14623.0
68	10719.5
69	8085.0
70	5972.5
71	4308.5
72	3168.0
73	2345.0
74	1774.0
75	1286.0
76	827.5
77	501.5
78	289.5
79	151.5
80	92.0
81	58.5
82	43.0
83	30.0
84	20.0
85	15.0
86	11.5
87	9.0
88	11.0
89	11.0
90	7.5
91	4.0
92	4.0
93	5.5
94	4.5
95	3.5
96	1.5
97	0.5
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3691713523030613
2	0.023668601563530622
3	0.017378093691005275
4	0.015522620145871899
5	0.022152543910799693
6	0.022944514326405402
7	0.014527000194824722
8	0.008508025036221332
9	0.0
10-11	4.525545232032624E-5
12-13	0.0052835740583980885
14-15	0.0027153271392195743
16-17	0.005068610659876538
18-19	0.03571786574381749
20-21	0.07538426970258343
22-23	0.09416528241551882
24-25	0.061807634006485555
26-27	0.07108500173215244
28-29	0.05467990026603417
30-31	0.04965654505847797
32-33	0.0181587502435309
34-35	0.32813597091160546
36-37	0.4990771281885577
38-39	0.454025325403673
40-41	0.46308772973081824
42-43	0.4158976068237981
44-45	0.39047535648285486
46-47	0.3894797365318077
48-49	0.3797837558721778
50-51	0.3765593048943545
52-53	0.4350632908814563
54-55	0.4358326335709018
56-57	0.407559289733778
58-59	0.39383557381763906
60-61	0.4076611145014988
62-63	0.3884049195392
64-65	0.4357647503924213
66-67	0.45494174831315953
68-69	0.39978666579776195
70-71	0.36027865592211716
72-73	0.34662282318445875
74-75	0.32988961968901814
76-77	0.32818122636392577
78-79	0.32720823413903877
80-81	0.3427987374633912
82-83	0.33949508944400736
84-85	0.3384089585883195
86-87	0.3253075051415851
88-89	0.3661505508606795
90-91	0.3464531152382575
92-93	0.3356031205444593
94-95	0.3432626058496745
96-97	0.34329654743891475
98	0.33771881294043454
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
98	4419357.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.794292580445145
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.80822605866065	40.99063826082333
2	12.302079689631586	13.481675077232499
3	5.029387562924777	8.267452008700566
4	2.551335709178691	5.5919454127869885
5	1.4783410991858286	4.050232736124258
6	0.9329816678112618	3.0673242286945177
7	0.6208918903565502	2.381493233071574
8	0.42302504640842153	1.8543486529407547
9	0.30194207977348425	1.4890232395310763
>10	1.4646699367823244	13.7249255508644
>50	0.06516000283463659	2.4338692925013743
>100	0.020506371498138543	1.863036100448792
>500	9.132420622419486E-4	0.3542408941279448
>1k	5.396428914456463E-4	0.4497953121520492
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	9.051090464065248E-5
2	0.0	0.0	0.0	2.262772616016312E-5	9.051090464065248E-5
3	0.0	0.0	0.0	2.262772616016312E-5	9.051090464065248E-5
4	0.0	0.0	0.0	2.262772616016312E-5	9.051090464065248E-5
5	0.0	0.0	0.0	4.525545232032624E-5	1.8102180928130496E-4
6	0.0	0.0	0.0	4.525545232032624E-5	2.262772616016312E-4
7	0.0	0.0	0.0	4.525545232032624E-5	2.262772616016312E-4
8	0.0	0.0	0.0	4.525545232032624E-5	2.262772616016312E-4
9	0.0	0.0	0.0	4.525545232032624E-5	2.262772616016312E-4
10-11	0.0	0.0	0.0	6.788317848048935E-5	2.4890498776179433E-4
12-13	0.0	0.0	0.0	6.788317848048935E-5	5.65693154004078E-4
14-15	0.0	1.131386308008156E-5	0.0	6.788317848048935E-5	7.127733740451383E-4
16-17	0.0	2.262772616016312E-5	0.0	6.788317848048935E-5	9.390506356467695E-4
18-19	0.0	2.262772616016312E-5	0.0	9.051090464065248E-5	9.956199510471773E-4
20-21	0.0	2.262772616016312E-5	0.0	1.4708022004106025E-4	0.0012332110757288899
22-23	0.0	2.262772616016312E-5	0.0	2.9416044008212056E-4	0.0013124081172894608
24-25	0.0	4.525545232032624E-5	0.0	7.240872371252198E-4	0.0013124081172894608
26-27	0.0	4.525545232032624E-5	0.0	0.001493429926570766	0.0013802912957699503
28-29	0.0	4.525545232032624E-5	0.0	0.005487223593839557	0.0014255467480902765
30-31	0.0	4.525545232032624E-5	0.0	0.021496339852154964	0.0014255467480902765
32-33	0.0	4.525545232032624E-5	0.0	0.05831165031474035	0.001516057652730929
34-35	0.0	4.525545232032624E-5	0.0	0.10984629664451187	0.0016291962835317446
36-37	0.0	4.525545232032624E-5	0.0	0.19397618250799833	0.0016291962835317446
38-39	0.0	4.525545232032624E-5	0.0	0.3334195449700036	0.0016631378727719893
40-41	0.0	4.525545232032624E-5	0.0	0.49509464838436906	0.0016744517358520709
42-43	0.0	4.525545232032624E-5	0.0	0.6655266818227177	0.0017310210512524785
44-45	0.0	4.525545232032624E-5	0.0	0.876009790564555	0.0018894151343736205
46-47	0.0	4.525545232032624E-5	0.0	1.1222673343656102	0.002059123080574844
48-49	0.0	4.525545232032624E-5	0.0	1.3841945785325782	0.0022514587529362303
50-51	0.0	4.525545232032624E-5	0.0	1.6751531953630359	0.0023759112468171276
52-53	0.0	4.525545232032624E-5	0.0	1.9744840708727538	0.002443794425297617
54-55	0.0	4.525545232032624E-5	0.0	2.3076547108549956	0.002511677603778106
56-57	0.0	4.525545232032624E-5	0.0	2.6948603609077066	0.002568246919178514
58-59	0.0	4.525545232032624E-5	0.0	3.110757967731505	0.0026021885084187586
60-61	0.0	4.525545232032624E-5	0.0	3.558877456607375	0.0026021885084187586
62-63	0.0	4.525545232032624E-5	0.0	4.016025408221151	0.002749268728459819
64-65	0.0	4.525545232032624E-5	0.0	4.499783565799278	0.002805838043860227
66-67	0.0	4.525545232032624E-5	0.0	5.055599717334445	0.002805838043860227
68-69	0.0	5.65693154004078E-5	0.0	5.660393582143285	0.0028171519069403084
70-71	0.0	6.788317848048935E-5	0.0	6.306381222426701	0.002885035085420798
72-73	0.0	6.788317848048935E-5	0.0	6.970459729775168	0.003009487579301695
74-75	0.0	6.788317848048935E-5	0.0	7.646802464702445	0.0031113123470224288
76-77	0.0	6.788317848048935E-5	1.131386308008156E-5	8.377960866252714	0.0031452539362626735
78-79	0.0	6.788317848048935E-5	2.262772616016312E-5	9.18187872127099	0.003156567799342755
80-81	0.0	6.788317848048935E-5	2.262772616016312E-5	10.013029044723023	0.0032018232516630813
82-83	0.0	6.788317848048935E-5	2.262772616016312E-5	10.865551255533328	0.0033262757455439786
84-85	0.0	6.788317848048935E-5	2.262772616016312E-5	11.730948642528766	0.0033262757455439786
86	0.0	6.788317848048935E-5	2.262772616016312E-5	12.405039918703105	0.0033262757455439786
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	6010	0.0	42.26996	1
GGTATCA	3565	0.0	37.4374	1
TCAACGC	7275	0.0	34.169327	4
ATCAACG	7405	0.0	33.6314	3
CAACGCA	7495	0.0	32.860397	5
TATCAAC	8025	0.0	30.919481	2
AACGCAG	8265	0.0	29.73879	6
GTACATG	12495	0.0	25.009254	1
ACGCAGA	9655	0.0	24.83658	7
TACATGG	12870	0.0	23.484802	2
CGCAGAG	10080	0.0	23.33348	8
ACATGGG	13140	0.0	21.884789	3
GCAGAGT	11735	0.0	19.221836	9
CATGGGG	9455	0.0	18.723885	4
TAAGGCG	1180	0.0	18.656467	5
GAGTACA	9435	0.0	18.047916	1
AGAGTAC	9750	0.0	17.775314	10-11
GTATATA	1495	0.0	17.546919	1
AGTACAT	9285	0.0	16.74549	2
GTATTAA	2495	0.0	16.232271	1
>>END_MODULE