FastQCFastQC Report
Mon 15 Aug 2016
SRR2920872_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920872_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4419357
Sequences flagged as poor quality0
Sequence length101
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA35800.042.9018631
GTATCAA62350.042.70791
TCAACGC75800.034.196814
ATCAACG77650.033.382083
CAACGCA79350.032.7824755
TATCAAC82950.031.8178882
AACGCAG86400.030.2177336
GTACATG130100.026.4251631
ACGCAGA101150.025.3886387
TACATGG135350.024.9007852
CGCAGAG104500.024.4359788
ACATGGG137550.023.6424333
TACACCG5300.022.3909325
CATGGGG97600.020.3810424
GCAGAGT124350.019.8098149
GTATATA14100.019.2226121
GAGTACA100350.018.4326971
AGAGTAC105050.017.64186310-11
GTATAGA13800.017.5730741
AGTACAT98050.017.3804022
Produced by FastQC (version 0.11.5)