##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920872_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4419357
Sequences flagged as poor quality	0
Sequence length	101
%GC	48
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.255949225192715	31.0	30.0	34.0	27.0	34.0
2	30.265806088985343	31.0	30.0	34.0	26.0	34.0
3	30.40557461187227	31.0	30.0	34.0	27.0	34.0
4	34.05042860307506	35.0	35.0	37.0	30.0	37.0
5	33.57183228238859	35.0	35.0	37.0	28.0	37.0
6	33.51564175512411	35.0	35.0	37.0	28.0	37.0
7	33.330554422283605	35.0	33.0	37.0	27.0	37.0
8	33.260482463851645	35.0	33.0	37.0	27.0	37.0
9	34.59454644646269	38.0	34.0	39.0	27.0	39.0
10-11	34.40781690639611	37.5	34.0	39.0	27.0	39.0
12-13	34.22730523920109	37.0	34.0	39.0	26.5	39.0
14-15	35.130130808622155	38.0	34.0	40.0	26.0	41.0
16-17	34.99948714258658	38.0	34.0	40.0	25.5	41.0
18-19	34.90613148021308	38.0	34.0	40.0	25.0	41.0
20-21	34.791780795260486	38.0	34.0	40.0	25.0	41.0
22-23	34.6474223512606	38.0	34.0	40.0	24.5	41.0
24-25	34.47405957472999	38.0	33.0	40.0	24.0	41.0
26-27	34.29102220074097	38.0	33.0	40.0	23.0	41.0
28-29	34.234502666338116	38.0	33.0	40.0	23.5	41.0
30-31	34.234266432877	38.0	33.0	40.0	23.0	41.0
32-33	34.0957417560971	38.0	33.0	40.0	22.5	41.0
34-35	33.93073754847141	38.0	33.0	40.0	21.5	41.0
36-37	33.74436812866668	38.0	33.0	40.0	19.5	41.0
38-39	33.56510913239188	38.0	32.0	40.0	18.5	41.0
40-41	33.34291391258955	38.0	32.0	40.0	17.5	41.0
42-43	33.381025226067955	38.0	32.0	40.0	17.5	41.0
44-45	33.243209136532755	38.0	32.0	40.0	15.5	41.0
46-47	33.01123432662263	38.0	32.0	40.0	15.0	41.0
48-49	32.751922395045256	37.0	31.0	40.0	12.5	41.0
50-51	32.49518753520026	37.0	31.0	40.0	9.0	41.0
52-53	32.236418555912095	37.0	31.0	40.0	8.5	41.0
54-55	31.9535743548213	36.5	30.5	40.0	7.5	41.0
56-57	31.626794802954365	36.0	30.0	39.0	6.5	41.0
58-59	31.294745027387467	36.0	29.5	39.0	2.0	40.5
60-61	30.96347194852102	35.0	29.0	39.0	2.0	40.0
62-63	30.636181123181494	35.0	29.0	39.0	2.0	40.0
64-65	30.225636331258144	35.0	28.0	38.0	2.0	40.0
66-67	29.79210584254678	34.0	28.0	38.0	2.0	40.0
68-69	29.341978934944606	34.0	28.0	37.0	2.0	39.5
70-71	28.910777744364168	34.0	26.5	36.5	2.0	39.0
72-73	28.460330541298205	34.0	26.0	36.0	2.0	39.0
74-75	28.007022627952438	33.0	26.0	36.0	2.0	38.5
76-77	26.559722375902197	31.5	24.5	34.5	2.0	36.0
78-79	27.395705415968884	33.0	26.0	35.0	2.0	37.0
80-81	27.419500053967127	33.0	26.0	35.0	2.0	36.5
82-83	27.071873804266097	33.0	25.5	35.0	2.0	36.0
84-85	26.741981129834045	33.0	25.0	35.0	2.0	36.0
86-87	26.201413802958214	32.5	24.0	35.0	2.0	35.0
88-89	25.825693534149877	32.0	23.0	35.0	2.0	35.0
90-91	25.451877275359287	32.0	20.5	34.5	2.0	35.0
92-93	25.14166891246849	32.0	19.5	34.0	2.0	35.0
94-95	24.753909222540745	32.0	17.5	34.0	2.0	35.0
96-97	24.213737994011346	31.5	6.0	34.0	2.0	35.0
98-99	23.603067143025555	31.0	2.0	34.0	2.0	35.0
100-101	21.401297971627997	28.0	2.0	33.0	2.0	34.5
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	103410.0
3	46730.0
4	25077.0
5	17796.0
6	15026.0
7	15163.0
8	16896.0
9	19654.0
10	22040.0
11	25174.0
12	27784.0
13	30456.0
14	32694.0
15	34683.0
16	36604.0
17	38098.0
18	37425.0
19	36293.0
20	37375.0
21	38900.0
22	41466.0
23	47229.0
24	55013.0
25	64439.0
26	76334.0
27	90268.0
28	106996.0
29	126369.0
30	148296.0
31	176041.0
32	210529.0
33	257865.0
34	322767.0
35	419383.0
36	559803.0
37	656616.0
38	377005.0
39	25659.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.28352891702967	19.10440689569965	13.763991516960978	24.848072670309705
2	16.02056588775245	20.2006309967717	38.41033887961529	25.368464235860554
3	19.461529235246594	23.96337475474166	29.00556937274968	27.569526637262072
4	13.80076432913451	16.319741865226508	35.84396768608147	34.03552611955752
5	14.62707109181484	36.87741652836382	34.092471542500355	14.403040837320995
6	33.9938263676666	35.16465096007979	16.803384058419134	14.038138613834473
7	29.652417123018697	31.21017376311358	20.55608818971434	18.581320924153374
8	26.82651864857976	34.80096287275919	19.574138370479453	18.798380108181586
9	26.534068120378784	15.910307337069204	19.228722861862604	38.32690168068941
10-11	25.768034004888424	25.35255537874409	27.49103398234808	21.38837663401941
12-13	26.38090150680047	22.489791026977603	27.423625826696878	23.705681639525046
14-15	23.41073635131968	24.191287168054938	24.74518261049223	27.652793870133152
16-17	23.754224024794834	27.2556473547257	25.502694047012003	23.487434573467464
18-19	23.69449977257938	26.470458021844834	26.851870199157506	22.98317200641828
20-21	24.39036729737934	25.69808368651477	26.61080955908967	23.300739457016217
22-23	24.0636122787134	25.582898730881954	26.482748593602423	23.87074039680222
24-25	23.870632075636163	25.780067555252874	26.534092671461813	23.81520769764915
26-27	23.755682875052205	25.993340576709993	26.402606075900124	23.84837047233767
28-29	23.850192847948303	26.044495432417534	26.356350321028422	23.748961398605736
30-31	24.06974065044257	25.84009991209257	26.246581251906846	23.843578185558012
32-33	23.85648912810486	25.88417119157727	26.114956911020514	24.14438276929735
34-35	24.08001148815126	25.911642854289575	26.230531844143627	23.77781381341554
36-37	24.28398752770173	25.62572263836661	26.174455327156785	23.91583450677488
38-39	23.945628182117478	25.799236546448405	26.2663649128708	23.988770358563322
40-41	24.15918409657359	25.618160781555126	26.395060236273583	23.827594885597705
42-43	24.056318807994753	25.54996891104939	26.229659933839095	24.164052347116762
44-45	23.905873464490526	25.664094479171073	26.36771333942486	24.062318716913538
46-47	24.04192214504049	25.63134697011401	26.171337408915097	24.155393475930406
48-49	23.848779325963218	25.662209936812236	26.207223511800535	24.281787225424008
50-51	23.848195611976543	25.80276966922184	26.223935551888555	24.125099166913063
52-53	24.036109611918413	25.602647178293047	26.255220302124098	24.10602290766444
54-55	23.714305273538166	25.521930973458336	26.397891286280288	24.365872466723204
56-57	23.64174275382857	25.756591910655303	26.25501812073224	24.346647214783886
58-59	23.601831966961324	25.59062650586324	26.30952611279298	24.49801541438245
60-61	23.541230772120812	25.65131401234826	26.38508232642613	24.422372889104807
62-63	23.4830522292296	25.701504294776466	26.350283536827877	24.465159939166057
64-65	23.34216633269481	25.53324424902722	26.290547596930097	24.834041821347874
66-67	23.3597150117734	25.65209822740293	26.142784769781507	24.845401991042166
68-69	23.521498877157992	25.506637796228777	26.215108014445487	24.756755312167744
70-71	23.33354834402163	25.340073352593766	26.17208550182929	25.154292801555318
72-73	23.345636922993336	25.321511926671963	26.156933587781072	25.175917562553625
74-75	23.30238017185884	25.571082149022455	25.96910798616478	25.157429692953926
76-77	23.27683330048184	25.33483006099944	26.191131276156916	25.197205362361807
78-79	23.230853283285068	25.412334859126023	26.107201798114133	25.249610059474776
80-81	23.269767354492167	25.447940540590697	25.994073703251996	25.28821840166514
82-83	23.250267634181725	25.416890944544708	26.04240981992556	25.290431601348008
84-85	23.148661846381444	25.34417771153491	26.000606731780763	25.50655371030289
86-87	23.136542729402663	25.335754720030945	26.027460657665113	25.500241892901283
88-89	23.08493256233965	25.28858755693425	25.892288727359496	25.7341911533666
90-91	22.933395742162897	25.296370093132246	25.9135118897442	25.85672227496066
92-93	22.996114604409502	25.173389468329827	25.927862442008596	25.902633485252075
94-95	22.769301751670994	25.13100224481532	26.03878288717803	26.060913116335648
96-97	22.940570755272557	24.934391101334537	25.9948933959347	26.13014474745821
98-99	22.764437461793197	24.99676129640016	25.880075981966442	26.3587252598402
100-101	22.957493147130958	24.80653168623856	25.92107119618106	26.314903970449414
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	50.0
1	37.5
2	49.0
3	84.5
4	82.0
5	79.5
6	155.0
7	341.5
8	515.5
9	569.5
10	498.0
11	435.0
12	472.5
13	512.5
14	555.5
15	603.5
16	690.0
17	794.0
18	889.0
19	1033.0
20	1229.5
21	1412.5
22	1654.5
23	2081.5
24	2558.5
25	3233.5
26	4263.5
27	5682.5
28	7694.0
29	10672.0
30	14800.0
31	20810.0
32	29131.0
33	37760.0
34	46794.0
35	57623.0
36	71443.5
37	85867.5
38	98395.0
39	110221.5
40	121892.5
41	132547.5
42	142488.0
43	153026.5
44	164737.5
45	177149.5
46	187761.0
47	194944.0
48	199918.5
49	202422.5
50	200912.0
51	198167.5
52	194064.5
53	191100.0
54	186240.5
55	174265.0
56	160257.5
57	144376.0
58	129876.5
59	115285.5
60	96633.5
61	79829.5
62	64725.0
63	48824.0
64	35652.5
65	26599.5
66	19801.0
67	14715.5
68	10855.5
69	8019.5
70	5942.5
71	4318.5
72	3071.0
73	2274.0
74	1729.0
75	1265.0
76	871.5
77	520.5
78	260.0
79	123.0
80	62.5
81	33.0
82	20.5
83	9.0
84	2.5
85	4.0
86	5.5
87	2.5
88	0.5
89	1.0
90	1.0
91	0.5
92	0.5
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.5
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1855926099656579
2	0.0
3	0.012377366209609227
4	0.01312408117289461
5	0.00703722283581073
6	0.10807002014093904
7	0.1421021202858244
8	0.1240451948100142
9	0.14226051436894552
10-11	0.11428133097190384
12-13	0.1154692865953124
14-15	0.11690614720648275
16-17	0.0668196753509617
18-19	0.07988718720845589
20-21	0.037471514521230126
22-23	0.08443536016664868
24-25	0.05720289173289237
26-27	0.06581274153683443
28-29	0.03976822872648668
30-31	0.024279550169855026
32-33	0.031814582981189346
34-35	0.020172617871785422
36-37	0.024562396746857063
38-39	0.03182589684426943
40-41	0.024641593788417633
42-43	0.013225905940615344
44-45	0.027108015939875416
46-47	0.033964216966404844
48-49	0.00870036070858272
50-51	0.015171890390389372
52-53	0.007059850561970894
54-55	0.012433935525009633
56-57	0.0056003622246403725
58-59	0.003903282762628138
60-61	0.0226390400232432
62-63	0.01001276882587218
64-65	0.007286127823572523
66-67	0.04007370302964888
68-69	0.09047696305141223
70-71	0.02199414982767855
72-73	0.044056182833837595
74-75	0.08631346143794222
76-77	0.24048747363021364
78-79	0.08190105483671041
80-81	0.016880283715481687
82-83	0.012343424620368982
84-85	0.013587949559177952
86-87	0.2582841802551819
88-89	0.6211084553703174
90-91	0.26838745998569474
92-93	0.35496114027447884
94-95	0.001414232885010195
96-97	0.0040164213934289535
98-99	0.0034054727871045496
100-101	0.005034669070636294
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4419357.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.29299931219028
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.95129715018726	36.905978352357536
2	13.412066880890757	13.758902745931593
3	5.617172678826261	8.643649030544683
4	2.8939716369991557	5.937619407443835
5	1.617395851314446	4.148054214450565
6	1.0858313781154987	3.3417328878499735
7	0.7162813281768127	2.571815237145559
8	0.5178791980080232	2.1250861877778546
9	0.36961363109294554	1.7062732552883948
>10	1.7111047737039802	15.00194841153859
>50	0.0803461515448495	2.79291713956791
>100	0.025138052079031154	2.0961021387415184
>500	0.0012822649048034474	0.4762515631293437
>1k	6.190241562310228E-4	0.4936694282326476
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	6.788317848048935E-5
2	0.0	0.0	0.0	0.0	6.788317848048935E-5
3	0.0	0.0	0.0	0.0	6.788317848048935E-5
4	0.0	0.0	0.0	0.0	6.788317848048935E-5
5	0.0	0.0	0.0	2.262772616016312E-5	1.357663569609787E-4
6	0.0	0.0	0.0	4.525545232032624E-5	2.0364953544146807E-4
7	0.0	0.0	0.0	6.788317848048935E-5	2.0364953544146807E-4
8	0.0	0.0	0.0	6.788317848048935E-5	2.0364953544146807E-4
9	0.0	0.0	0.0	9.051090464065248E-5	2.0364953544146807E-4
10-11	0.0	0.0	0.0	9.051090464065248E-5	2.4890498776179433E-4
12-13	0.0	0.0	0.0	9.051090464065248E-5	5.430654278439148E-4
14-15	0.0	0.0	0.0	9.051090464065248E-5	7.919704156057092E-4
16-17	0.0	0.0	0.0	9.051090464065248E-5	0.0010182476772073405
18-19	0.0	0.0	0.0	1.2445249388089716E-4	0.0010861308556878297
20-21	0.0	0.0	0.0	1.9233567236138653E-4	0.0012105833495687267
22-23	0.0	0.0	0.0	3.394158924024468E-4	0.0013576635696097871
24-25	0.0	0.0	0.0	7.240872371252198E-4	0.0014029190219301134
26-27	0.0	0.0	0.0	0.0015047437896508475	0.001516057652730929
28-29	0.0	0.0	0.0	0.005464595867679393	0.0016065685573715815
30-31	0.0	0.0	0.0	0.02169998938759643	0.0016631378727719893
32-33	0.0	0.0	0.0	0.058820774153344024	0.00174233491433256
34-35	0.0	0.0	0.0	0.11083060273247895	0.0018554735451333758
36-37	2.262772616016312E-5	0.0	0.0	0.19588822536853212	0.001878101271293539
38-39	2.262772616016312E-5	0.0	0.0	0.33699472570330935	0.001878101271293539
40-41	2.262772616016312E-5	0.0	0.0	0.5003442808535269	0.001878101271293539
42-43	2.262772616016312E-5	0.0	0.0	0.6728354373724503	0.0019120428605337836
44-45	4.525545232032624E-5	0.0	0.0	0.8849816839870597	0.0020025537651744358
46-47	4.525545232032624E-5	0.0	0.0	1.133049445880928	0.0020364953544146805
48-49	4.525545232032624E-5	0.0	0.0	1.3941960334953705	0.00210437853289517
50-51	4.525545232032624E-5	0.0	0.0	1.6828692499836515	0.0022175171636959856
52-53	4.525545232032624E-5	0.0	0.0	1.9792811488187083	0.002387225109897209
54-55	4.525545232032624E-5	0.0	0.0	2.3095780675786095	0.002443794425297617
56-57	4.525545232032624E-5	0.0	0.0	2.6946227697830247	0.0024664221514577797
58-59	4.525545232032624E-5	0.0	0.0	3.107861618783004	0.002489049877617943
60-61	4.525545232032624E-5	0.0	0.0	3.553763590495178	0.0025229914668581876
62-63	4.525545232032624E-5	0.0	0.0	4.008411178368256	0.002647443960739085
64-65	4.525545232032624E-5	0.0	0.0	4.489114592914762	0.0026587578238191664
66-67	4.525545232032624E-5	0.0	0.0	5.040914323056499	0.0026813855499793295
68-69	4.525545232032624E-5	0.0	0.0	5.64037935835462	0.002692699413059411
70-71	4.525545232032624E-5	1.131386308008156E-5	0.0	6.281038169127319	0.0027605825915399005
72-73	4.525545232032624E-5	2.262772616016312E-5	0.0	6.943340399972213	0.002952918263901287
74-75	4.525545232032624E-5	2.262772616016312E-5	0.0	7.620644813261295	0.0031452539362626735
76-77	4.525545232032624E-5	2.262772616016312E-5	0.0	8.355785694615754	0.003213137114743163
78-79	4.525545232032624E-5	2.262772616016312E-5	0.0	9.160812308215878	0.003258392567063489
80-81	4.525545232032624E-5	2.262772616016312E-5	0.0	9.99191737621559	0.00333758960862406
82-83	4.525545232032624E-5	2.262772616016312E-5	0.0	10.84377206910417	0.0035299252809854464
84-85	4.525545232032624E-5	2.262772616016312E-5	0.0	11.708897923385688	0.003563866870225691
86-87	4.525545232032624E-5	2.262772616016312E-5	0.0	12.612094926931679	0.003744888679506996
88-89	4.525545232032624E-5	2.262772616016312E-5	0.0	13.552333518201856	0.00391459662570822
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	3580	0.0	42.901863	1
GTATCAA	6235	0.0	42.7079	1
TCAACGC	7580	0.0	34.19681	4
ATCAACG	7765	0.0	33.38208	3
CAACGCA	7935	0.0	32.782475	5
TATCAAC	8295	0.0	31.817888	2
AACGCAG	8640	0.0	30.217733	6
GTACATG	13010	0.0	26.425163	1
ACGCAGA	10115	0.0	25.388638	7
TACATGG	13535	0.0	24.900785	2
CGCAGAG	10450	0.0	24.435978	8
ACATGGG	13755	0.0	23.642433	3
TACACCG	530	0.0	22.390932	5
CATGGGG	9760	0.0	20.381042	4
GCAGAGT	12435	0.0	19.809814	9
GTATATA	1410	0.0	19.222612	1
GAGTACA	10035	0.0	18.432697	1
AGAGTAC	10505	0.0	17.641863	10-11
GTATAGA	1380	0.0	17.573074	1
AGTACAT	9805	0.0	17.380402	2
>>END_MODULE