FastQCFastQC Report
Mon 15 Aug 2016
SRR2920871_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920871_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3524428
Sequences flagged as poor quality0
Sequence length98
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA76800.040.6914831
GGTATCA43800.035.8323441
ATCAACG96600.031.7664093
TCAACGC96550.031.7353534
TATCAAC98050.031.3447532
CAACGCA99550.030.8250665
AACGCAG104700.029.5224386
GTACATG127250.026.6927621
ACGCAGA119250.025.4167757
TACATGG133000.025.3152392
CGCAGAG122600.024.5345678
ACATGGG139750.023.3365633
GTATAGG6900.022.0120161
CATGGGG96750.020.8603134
GCAGAGT136150.020.778799
TATACGG1457.779552E-418.9802685
AGAGTAC112100.018.77426110-11
GTATAGT5750.018.410051
TAAGGTG15550.018.2885995
GTAAGGT11450.018.0271134
Produced by FastQC (version 0.11.5)