##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920871_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3524428
Sequences flagged as poor quality	0
Sequence length	98
%GC	48
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.670892978945805	31.0	30.0	33.0	22.0	34.0
2	28.70218571637724	31.0	28.0	34.0	20.0	34.0
3	28.768349927988314	31.0	28.0	34.0	22.0	34.0
4	32.2648636885191	35.0	32.0	37.0	23.0	37.0
5	32.22628920210599	35.0	32.0	37.0	23.0	37.0
6	32.35442034849343	35.0	32.0	37.0	25.0	37.0
7	32.19445680263578	35.0	32.0	37.0	23.0	37.0
8	32.13830442840654	35.0	32.0	37.0	23.0	37.0
9	33.39608526546719	37.0	33.0	39.0	22.0	39.0
10-11	33.20661608068032	37.0	32.5	39.0	21.5	39.0
12-13	33.037136238845	37.0	32.0	39.0	19.0	39.0
14-15	33.794285200321866	38.0	32.5	40.0	18.0	41.0
16-17	33.69652508151677	38.0	32.0	40.0	17.5	41.0
18-19	33.59826261169188	38.0	32.0	40.0	17.5	41.0
20-21	33.50587868442766	38.0	32.0	40.0	18.0	41.0
22-23	33.28680909356071	38.0	32.0	40.0	17.0	41.0
24-25	33.08918638712438	38.0	32.0	40.0	16.0	41.0
26-27	33.11265629486544	38.0	32.0	40.0	15.5	41.0
28-29	32.949297871881626	38.0	32.0	40.0	11.5	41.0
30-31	32.69760284505741	38.0	31.0	40.0	9.5	41.0
32-33	32.449132029367604	37.0	31.0	40.0	9.0	41.0
34-35	32.11402446013935	37.0	30.0	40.0	8.0	41.0
36-37	31.804361303451227	37.0	30.0	40.0	7.5	41.0
38-39	31.595040670429356	37.0	30.0	40.0	2.0	41.0
40-41	31.389819426017496	37.0	30.0	40.0	2.0	41.0
42-43	31.146275934704867	36.0	30.0	40.0	2.0	41.0
44-45	30.853349536435417	36.0	29.0	39.0	2.0	40.5
46-47	30.583013186820672	36.0	28.5	39.0	2.0	40.0
48-49	30.264906390483787	35.5	28.0	39.0	2.0	40.0
50-51	29.116926491334198	34.0	26.0	38.0	2.0	39.5
52-53	29.565565674770486	34.5	27.5	38.5	2.0	39.5
54-55	30.213215165694972	35.0	28.0	39.0	2.0	40.0
56-57	30.15713415056287	35.0	28.0	39.0	2.0	40.0
58-59	29.907952581241553	35.0	28.0	39.0	2.0	40.0
60-61	29.576170658047204	35.0	28.0	39.0	2.0	40.0
62-63	29.180932905992123	34.5	26.5	38.5	2.0	40.0
64-65	28.751383061308104	34.0	26.0	38.0	2.0	40.0
66-67	28.35280916506168	34.0	26.0	37.5	2.0	40.0
68-69	27.90066033410244	34.0	25.5	37.0	2.0	39.5
70-71	27.562204278254512	34.0	25.0	36.0	2.0	39.0
72-73	27.10228283852018	33.0	24.0	36.0	2.0	39.0
74-75	26.658779098338794	33.0	23.0	35.0	2.0	38.5
76-77	26.239240807302632	32.5	21.5	35.0	2.0	37.0
78-79	25.77257259901465	32.0	20.0	35.0	2.0	37.0
80-81	25.31867738537998	32.0	18.5	35.0	2.0	36.0
82-83	24.839030191565836	32.0	16.5	35.0	2.0	36.0
84-85	24.434270610720375	31.0	9.0	34.0	2.0	35.0
86-87	23.969985484169346	31.0	4.5	34.0	2.0	35.0
88-89	23.42971795706991	31.0	2.0	34.0	2.0	35.0
90-91	22.91275818373932	30.5	2.0	34.0	2.0	35.0
92-93	22.357621293441092	30.0	2.0	34.0	2.0	35.0
94-95	21.70665977571396	30.0	2.0	34.0	2.0	35.0
96-97	21.047301434445533	29.0	2.0	34.0	2.0	35.0
98	20.643644869465344	29.0	2.0	34.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	170473.0
3	29760.0
4	22009.0
5	17888.0
6	16876.0
7	17288.0
8	19906.0
9	22570.0
10	25059.0
11	26963.0
12	28390.0
13	28551.0
14	27249.0
15	28196.0
16	31027.0
17	33118.0
18	34185.0
19	33137.0
20	34385.0
21	36178.0
22	40046.0
23	44824.0
24	50739.0
25	58232.0
26	67219.0
27	77711.0
28	89281.0
29	103846.0
30	120429.0
31	141276.0
32	168547.0
33	204119.0
34	251895.0
35	318599.0
36	401874.0
37	430921.0
38	247816.0
39	23846.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.480452078695734	17.75668704958223	12.921257284070714	24.84160358765133
2	16.10580299990663	19.616941897916444	38.86171215415409	25.41554294802283
3	19.506114541265383	23.70707526193927	28.60841367007841	28.17839652671694
4	13.456518889025732	15.388555514376408	35.81059951224468	35.34432608435318
5	14.582849112339554	36.77392037519642	33.709982770955875	14.933247741508145
6	33.99270295474708	34.35707275873832	17.031670288890567	14.618553997624034
7	29.558440393865844	31.444652450197097	20.59117902371237	18.405728132224688
8	27.18921521807664	33.7957142018035	19.797562590252234	19.21750798986763
9	27.302870139494978	15.542976051716762	19.188276792716437	37.96587701607182
10-11	25.896606739194766	25.155805468520132	27.172757443975996	21.774830348309102
12-13	26.60287340650448	22.480534150680047	27.279400519089315	23.63719192372616
14-15	23.674679753643115	24.078147885633474	24.81661700221149	27.430555358511917
16-17	23.714103547040295	27.047324326364254	25.583786434158455	23.654785692436995
18-19	23.68183974052758	26.18252875481565	27.076828144367436	23.058803360289332
20-21	24.538128588998795	25.45384281528707	26.704188343046916	23.303840252667218
22-23	24.23698955256392	25.26252069280755	26.6614098628545	23.839079891774027
24-25	23.923236325448407	25.60525237693308	26.573201930220986	23.898309367397527
26-27	23.689066503879218	25.650707344600686	26.665478886136327	23.99474726538377
28-29	23.798636779142324	25.912239463568902	26.525149466010323	23.76397429127845
30-31	23.940550374022752	25.70338178225394	26.27397885181409	24.08208899190922
32-33	23.677099821002006	25.92827451751066	26.147144439249587	24.247481222237752
34-35	24.132904170081176	25.73122228126949	26.252343665719476	23.88352988292986
36-37	24.23220209178847	25.454517201284887	26.28219128556431	24.031089421362335
38-39	23.98906499555965	25.622632335555977	26.224131680618523	24.164170988265845
40-41	24.131244637884397	25.545517770157993	26.38541390544435	23.937823686513262
42-43	23.987354601492314	25.522346324548227	26.243874199566587	24.24642487439287
44-45	23.913380314184202	25.52419099874848	26.30090739387297	24.261521293194345
46-47	24.03329578957877	25.605391262269066	26.149678390675973	24.211634557476195
48-49	23.90685785891265	25.628257340586106	26.109161848887872	24.355722951613362
50-51	23.751029850206454	25.789100916919104	26.247375359290636	24.212493873583803
52-53	23.948726657960492	25.471086545883942	26.305591261379835	24.27459553477573
54-55	23.572852616403033	25.55085749733677	26.408547176351764	24.467742709908432
56-57	23.57945509724831	25.592590751486416	26.3428394076464	24.48511474361887
58-59	23.543558691807252	25.19239322402152	26.438831579932287	24.825216504238938
60-61	23.39388450884296	25.225845524851554	26.846646102797315	24.533623863508172
62-63	23.416152579913497	25.20943110359315	26.74810976190683	24.62630655458652
64-65	23.36658490906099	25.23536003385235	26.562241093989424	24.835813963097234
66-67	23.332293997808843	25.26454604218372	26.46522083124801	24.93793912875942
68-69	23.47765512715191	24.989602928726796	26.602402321651773	24.930339622469525
70-71	23.23598723426004	24.849002286836786	26.601664865015696	25.313345613887478
72-73	23.136389427200903	24.91170876318817	26.51107195597011	25.440829853640818
74-75	23.104253632460082	25.049521578428262	26.606715123188394	25.23950966592326
76-77	23.128647267894536	24.700750221915566	26.784870773766244	25.385731736423654
78-79	23.037240777048336	24.843347588176222	26.663074491801186	25.45633714297425
80-81	23.08033602019674	24.724580843045384	26.680058280851526	25.515024855906344
82-83	22.979242475246046	24.59078134463099	26.827600882196585	25.602375297926383
84-85	22.89299564233715	24.564038001268226	26.790524022657287	25.752442333737335
86-87	22.88960366653322	24.538100524821242	26.81735916305265	25.754936645592885
88-89	22.850559388398032	24.31316011767666	26.772863067124756	26.063417426800555
90-91	22.802214187809668	24.2693620082052	26.802337176100583	26.126086627884547
92-93	22.95522011618983	23.997586590117713	26.933830780056887	26.11336251363557
94-95	22.740742775699037	23.87503485590969	27.07464723114902	26.309575137242252
96-97	22.91129296394096	23.591374024797947	27.122406477466782	26.374926533794312
98	22.896990741729173	23.554347043248818	26.94924561446958	26.599416600552427
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	183.0
1	141.5
2	166.5
3	243.5
4	231.5
5	272.0
6	406.0
7	742.5
8	1155.5
9	1287.5
10	1268.5
11	1301.0
12	1417.5
13	1585.5
14	1793.0
15	1987.5
16	2143.0
17	2264.5
18	2354.0
19	2441.0
20	2504.5
21	2612.5
22	2875.5
23	3286.5
24	3851.5
25	4649.5
26	5720.5
27	7310.5
28	9499.0
29	12847.5
30	18277.0
31	25887.5
32	32644.5
33	38858.5
34	46675.5
35	54937.0
36	63408.5
37	71809.5
38	79688.0
39	87533.0
40	95485.0
41	103499.0
42	113455.0
43	124118.0
44	131910.0
45	139938.0
46	148125.5
47	153106.5
48	156613.5
49	159197.5
50	160194.0
51	159731.0
52	159627.5
53	159469.0
54	154037.0
55	145451.0
56	134511.5
57	121796.5
58	111554.5
59	98317.0
60	82269.5
61	67075.5
62	53993.0
63	41273.0
64	29799.5
65	22154.5
66	16452.0
67	12308.5
68	8675.5
69	5917.5
70	4170.0
71	3023.0
72	2477.0
73	2006.5
74	1550.5
75	1228.5
76	823.0
77	449.5
78	258.0
79	134.5
80	70.0
81	48.0
82	35.0
83	28.0
84	22.5
85	15.0
86	8.5
87	9.5
88	7.0
89	7.5
90	8.0
91	4.5
92	2.5
93	1.5
94	2.0
95	1.5
96	1.0
97	2.5
98	1.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.36394558209161887
2	0.022273117793866124
3	0.017449640055067092
4	0.015037901185667576
5	0.020627460682981748
6	0.022982452755454217
7	0.013817845051736053
8	0.007859431374396071
9	0.0
10-11	2.8373398463523723E-5
12-13	0.005078838324970747
14-15	0.00312107383098761
16-17	0.005504439301923603
18-19	0.036289576634846846
20-21	0.07494833204139792
22-23	0.09297962676496725
24-25	0.0606339525165502
26-27	0.06959994643102371
28-29	0.0535689762991328
30-31	0.04864619166571143
32-33	0.018315028708204565
34-35	0.31877513173768907
36-37	0.4877387195879729
38-39	0.4453488622834684
40-41	0.4544850965887231
42-43	0.40791016301084887
44-45	0.3828706388667892
46-47	0.38058658029047554
48-49	0.37248597502913944
50-51	0.36871231303349083
52-53	0.42676428628986035
54-55	0.42726082076297206
56-57	0.3988874222994483
58-59	0.3854384314277381
60-61	0.3990434759909977
62-63	0.38101218126742836
64-65	0.42760130154453435
66-67	0.4476187341605503
68-69	0.392233860359752
70-71	0.3510214990914838
72-73	0.33778530870824997
74-75	0.32098825681784393
76-77	0.3196972671877536
78-79	0.3188035051361526
80-81	0.3342953806972365
82-83	0.3292874758684246
84-85	0.3305642787992832
86-87	0.31598035198903196
88-89	0.3575473807380942
90-91	0.33760088161823704
92-93	0.3278829926444802
94-95	0.3334583654425626
96-97	0.33362860583334375
98	0.328251846824506
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
98	3524428.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.92003409535373
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.79373170004943	29.676756466033964
2	11.091563845248471	9.299174691272173
3	5.30342361666449	6.669590964980389
4	3.224583598453602	5.406986175619738
5	2.119776845137981	4.443055881136275
6	1.4523027007560685	3.6528347239481214
7	1.0701211304148759	3.1401659991205055
8	0.8236128383085412	2.7620702610612082
9	0.6444984756923069	2.431565826788251
>10	3.2839632571698765	23.84233185196252
>50	0.1406758109882454	4.013448317471676
>100	0.04846282939484003	3.4008226640102333
>500	0.0024625137944107125	0.6812231459767741
>1k	8.208379268011652E-4	0.579973030618145
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	2.8373398463523727E-5
3	0.0	0.0	0.0	0.0	2.8373398463523727E-5
4	0.0	0.0	0.0	0.0	2.8373398463523727E-5
5	0.0	0.0	0.0	0.0	8.512019539057118E-5
6	0.0	0.0	0.0	0.0	8.512019539057118E-5
7	0.0	0.0	0.0	0.0	8.512019539057118E-5
8	0.0	0.0	0.0	0.0	8.512019539057118E-5
9	0.0	0.0	0.0	0.0	8.512019539057118E-5
10-11	0.0	0.0	0.0	0.0	8.512019539057118E-5
12-13	0.0	0.0	0.0	1.4186699231761863E-5	2.1280048847642795E-4
14-15	0.0	0.0	0.0	5.674679692704745E-5	2.8373398463523727E-4
16-17	0.0	0.0	0.0	1.134935938540949E-4	3.404807815622847E-4
18-19	0.0	0.0	0.0	1.7024039078114236E-4	3.6885418002580845E-4
20-21	0.0	0.0	0.0	2.1280048847642795E-4	3.830408792575703E-4
22-23	0.0	0.0	0.0	4.965344731116652E-4	4.256009769528559E-4
24-25	0.0	0.0	0.0	0.0012909896300903295	4.256009769528559E-4
26-27	0.0	0.0	0.0	0.002496859064790088	4.681610746481415E-4
28-29	0.0	0.0	0.0	0.009434154989121639	4.8234777387990335E-4
30-31	0.0	0.0	0.0	0.03413319835161904	4.965344731116652E-4
32-33	0.0	0.0	0.0	0.08590046384831808	5.107211723434271E-4
34-35	0.0	0.0	0.0	0.15829519002799886	6.24214766197522E-4
36-37	0.0	1.4186699231761863E-5	0.0	0.2738742286691628	6.809615631245694E-4
38-39	0.0	2.8373398463523727E-5	0.0	0.46092585803994296	6.809615631245694E-4
40-41	0.0	2.8373398463523727E-5	0.0	0.6797415069906378	6.809615631245694E-4
42-43	0.0	2.8373398463523727E-5	0.0	0.9059200528426172	6.809615631245694E-4
44-45	0.0	2.8373398463523727E-5	0.0	1.1797233480156213	7.093349615880932E-4
46-47	0.0	2.8373398463523727E-5	0.0	1.507634714058565	7.377083600516169E-4
48-49	0.0	2.8373398463523727E-5	0.0	1.85786175799307	7.377083600516169E-4
50-51	0.0	2.8373398463523727E-5	0.0	2.238476711682009	7.660817585151406E-4
52-53	0.0	2.8373398463523727E-5	0.0	2.6150626427891277	8.228285554421881E-4
54-55	0.0	2.8373398463523727E-5	0.0	3.038720041947232	8.228285554421881E-4
56-57	2.8373398463523727E-5	5.674679692704745E-5	0.0	3.52939540827618	8.512019539057118E-4
58-59	2.8373398463523727E-5	5.674679692704745E-5	0.0	4.055792883270704	8.512019539057118E-4
60-61	2.8373398463523727E-5	5.674679692704745E-5	0.0	4.6144083522205595	8.512019539057118E-4
62-63	2.8373398463523727E-5	5.674679692704745E-5	0.0	5.17750681812765	8.795753523692355E-4
64-65	2.8373398463523727E-5	5.674679692704745E-5	0.0	5.775731551332585	8.795753523692355E-4
66-67	2.8373398463523727E-5	5.674679692704745E-5	0.0	6.4732206190621575	8.937620516009973E-4
68-69	2.8373398463523727E-5	5.674679692704745E-5	0.0	7.229924969385103	9.079487508327592E-4
70-71	2.8373398463523727E-5	5.674679692704745E-5	0.0	8.01845008608489	9.36322149296283E-4
72-73	2.8373398463523727E-5	5.674679692704745E-5	0.0	8.823077106412729	0.0010072556454550922
74-75	2.8373398463523727E-5	5.674679692704745E-5	0.0	9.647693185958119	0.0010781891416139016
76-77	2.8373398463523727E-5	5.674679692704745E-5	0.0	10.542703667091512	0.0010781891416139016
78-79	2.8373398463523727E-5	5.674679692704745E-5	0.0	11.517542704802029	0.0010923758408456635
80-81	2.8373398463523727E-5	5.674679692704745E-5	0.0	12.526018406391051	0.0011207492393091871
82-83	2.8373398463523727E-5	5.674679692704745E-5	0.0	13.548567313617983	0.0011633093370044727
84-85	4.256009769528559E-5	5.674679692704745E-5	0.0	14.568122827307013	0.0011633093370044727
86	5.674679692704745E-5	5.674679692704745E-5	0.0	15.352193320448027	0.0011633093370044727
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	7680	0.0	40.691483	1
GGTATCA	4380	0.0	35.832344	1
ATCAACG	9660	0.0	31.766409	3
TCAACGC	9655	0.0	31.735353	4
TATCAAC	9805	0.0	31.344753	2
CAACGCA	9955	0.0	30.825066	5
AACGCAG	10470	0.0	29.522438	6
GTACATG	12725	0.0	26.692762	1
ACGCAGA	11925	0.0	25.416775	7
TACATGG	13300	0.0	25.315239	2
CGCAGAG	12260	0.0	24.534567	8
ACATGGG	13975	0.0	23.336563	3
GTATAGG	690	0.0	22.012016	1
CATGGGG	9675	0.0	20.860313	4
GCAGAGT	13615	0.0	20.77879	9
TATACGG	145	7.779552E-4	18.980268	5
AGAGTAC	11210	0.0	18.774261	10-11
GTATAGT	575	0.0	18.41005	1
TAAGGTG	1555	0.0	18.288599	5
GTAAGGT	1145	0.0	18.027113	4
>>END_MODULE