FastQCFastQC Report
Mon 15 Aug 2016
SRR2920871_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920871_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3524428
Sequences flagged as poor quality0
Sequence length101
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA77150.040.806871
GGTATCA45650.039.170421
ATCAACG100150.030.9054893
TATCAAC104500.030.0715642
TCAACGC102800.030.062624
CAACGCA104450.029.4917935
AACGCAG108000.028.522796
GTACATG132150.025.9105431
ACGCAGA124400.024.5336237
TACATGG138100.024.198772
CGCAGAG126450.023.8717758
ACATGGG144900.022.4747433
TAAGGCG11650.022.0005115
TTACGTA1605.015634E-520.769074
GCAGAGT139300.020.5793789
CATGGGG98500.019.4226194
AGAGTAC116050.019.17976410-11
GTATTAA19900.018.879271
GTATAGG6600.018.013881
GAGTACA102150.017.4583851
Produced by FastQC (version 0.11.5)