##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920871_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3524428
Sequences flagged as poor quality	0
Sequence length	101
%GC	48
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.26685550109124	31.0	30.0	34.0	27.0	34.0
2	30.26720364269039	31.0	30.0	34.0	26.0	34.0
3	30.403332398902744	31.0	30.0	34.0	27.0	34.0
4	34.06394087210747	35.0	35.0	37.0	30.0	37.0
5	33.59022882578393	35.0	35.0	37.0	28.0	37.0
6	33.540872447954676	35.0	35.0	37.0	28.0	37.0
7	33.350003745288596	35.0	33.0	37.0	27.0	37.0
8	33.28062539509958	35.0	33.0	37.0	27.0	37.0
9	34.60825870183758	38.0	34.0	39.0	27.0	39.0
10-11	34.418460385628535	37.5	34.0	39.0	27.0	39.0
12-13	34.244573020075876	37.0	34.0	39.0	27.0	39.0
14-15	35.13323027169231	38.0	34.0	40.0	26.0	41.0
16-17	35.007265718011546	38.0	34.0	40.0	25.5	41.0
18-19	34.92590230244454	38.0	34.0	40.0	25.0	41.0
20-21	34.812383739999795	38.0	34.0	40.0	25.0	41.0
22-23	34.66993580234863	38.0	34.0	40.0	24.5	41.0
24-25	34.482300532171465	38.0	33.0	40.0	24.0	41.0
26-27	34.29743039154155	38.0	33.0	40.0	23.5	41.0
28-29	34.237831358733956	38.0	33.0	40.0	23.5	41.0
30-31	34.2100347914612	38.0	33.0	40.0	23.0	41.0
32-33	34.0575011037252	38.0	33.0	40.0	22.5	41.0
34-35	33.87759190994964	38.0	33.0	40.0	21.5	41.0
36-37	33.68427089445436	38.0	33.0	40.0	19.5	41.0
38-39	33.49582911042586	38.0	32.0	40.0	18.5	41.0
40-41	33.25418280640149	38.0	32.0	40.0	17.0	41.0
42-43	33.2666642643856	38.0	32.0	40.0	16.5	41.0
44-45	33.11121818916431	38.0	32.0	40.0	15.0	41.0
46-47	32.84226192165083	37.5	31.5	40.0	13.5	41.0
48-49	32.563892779197076	37.0	31.0	40.0	9.5	41.0
50-51	32.294815073538174	37.0	31.0	40.0	8.5	41.0
52-53	32.03090416373948	37.0	31.0	40.0	8.0	41.0
54-55	31.737243178183807	36.0	30.0	39.5	6.5	41.0
56-57	31.397950674549175	36.0	30.0	39.0	2.0	41.0
58-59	31.054238588502873	35.0	29.0	39.0	2.0	40.0
60-61	30.71500666207396	35.0	29.0	39.0	2.0	40.0
62-63	30.382527746346355	35.0	28.5	38.5	2.0	40.0
64-65	29.968244492439624	34.5	28.0	38.0	2.0	40.0
66-67	29.530061331938118	34.0	28.0	37.5	2.0	40.0
68-69	29.08226639897311	34.0	27.0	37.0	2.0	39.5
70-71	28.654197078220918	34.0	26.0	36.0	2.0	39.0
72-73	28.22491266667953	33.5	26.0	36.0	2.0	39.0
74-75	27.799489874669025	33.0	26.0	35.5	2.0	38.0
76-77	26.364620868975052	31.5	24.0	34.0	2.0	36.0
78-79	27.2142580299555	33.0	26.0	35.0	2.0	37.0
80-81	27.254861923693717	33.0	26.0	35.0	2.0	36.5
82-83	26.93233894975298	33.0	25.0	35.0	2.0	36.0
84-85	26.6320302188043	33.0	25.0	35.0	2.0	36.0
86-87	26.107054534806785	32.0	24.0	35.0	2.0	35.0
88-89	25.75017931987829	32.0	22.5	35.0	2.0	35.0
90-91	25.399751534149654	32.0	20.0	34.5	2.0	35.0
92-93	25.10057816474049	32.0	19.5	34.0	2.0	35.0
94-95	24.725193563324318	32.0	17.5	34.0	2.0	35.0
96-97	24.20419043884568	31.5	6.0	34.0	2.0	35.0
98-99	23.61920686136871	31.0	2.0	34.0	2.0	35.0
100-101	21.449115714663485	28.0	2.0	33.0	2.0	34.5
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	79410.0
3	36693.0
4	19830.0
5	13975.0
6	12297.0
7	12403.0
8	14019.0
9	16027.0
10	18624.0
11	21184.0
12	22834.0
13	25444.0
14	27696.0
15	29650.0
16	31327.0
17	32809.0
18	31032.0
19	29406.0
20	29509.0
21	30398.0
22	33562.0
23	37745.0
24	43972.0
25	51814.0
26	62561.0
27	73178.0
28	86461.0
29	101234.0
30	118944.0
31	142029.0
32	169883.0
33	207136.0
34	262274.0
35	339665.0
36	449239.0
37	512719.0
38	279360.0
39	18085.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.63167155641958	19.120407311064184	13.589808240766299	24.65811289174994
2	16.197720594661032	20.671808304780235	37.77597386015547	25.35449724040327
3	19.31007925938685	24.575432626399724	29.0458935998563	27.068594514357123
4	13.873912834967717	16.371047449757505	35.4724331355728	34.28260657970198
5	14.540569851548488	37.11753170452937	33.74880575089645	14.5930926930257
6	33.917617221529014	34.906805905592215	16.800102707927454	14.375474164951319
7	29.337645469969193	31.654282344363438	20.69788646771623	18.310185717951143
8	27.11495575346941	34.09525432877374	19.845087427735404	18.94470249002145
9	27.052144923442984	15.515046159817448	19.170978714287624	38.261830202451954
10-11	25.84818727470536	25.28853337272291	27.283212939475455	21.580066413096276
12-13	26.562630267419706	22.54629974839525	27.389041451936247	23.502028532248797
14-15	23.584350928085616	24.264787969017508	24.779444202515062	27.371416900381814
16-17	23.665166573895714	27.257666592705437	25.590827444471937	23.48633938892691
18-19	23.710626209666426	26.26184394053666	27.072533416325157	22.954996433471752
20-21	24.496819692404458	25.53176965430662	26.702009882873796	23.269400770415128
22-23	24.149644796150728	25.345314594722307	26.724741367241624	23.780299241885345
24-25	23.838569116640688	25.756743639975056	26.625424088014704	23.779263155369552
26-27	23.680502035052793	25.735713000985623	26.73870989676886	23.845075067192727
28-29	23.726594537595982	25.892609667812206	26.664036394265533	23.716759400326275
30-31	23.870901826897082	25.706951489209533	26.401979794797786	24.02016688909559
32-33	23.664958867939898	25.9331291997537	26.22884238039035	24.173069551916054
34-35	24.072260172264386	25.835174077105915	26.353462818962335	23.739102931667365
36-37	24.213383736258745	25.519489273059836	26.30229551836826	23.964831472313154
38-39	24.00252148434368	25.595861745798675	26.335206707647885	24.066410062209766
40-41	24.102957355486453	25.538418574633752	26.500889092535296	23.857734977344496
42-43	23.995367699494118	25.558967760902462	26.320967573045138	24.124696966558275
44-45	23.823469603362447	25.524502367173156	26.425266989006545	24.226761040457852
46-47	24.02080506301378	25.543847389802167	26.25003654268455	24.185311004499503
48-49	23.842839207226902	25.609001193350778	26.210755877263214	24.33740372215911
50-51	23.72634639246348	25.787254631399943	26.28254288740553	24.203856088731047
52-53	23.95918754174685	25.461691075864206	26.300543836959005	24.278577545429936
54-55	23.531716967976987	25.590558107739593	26.32502241063202	24.552702513651404
56-57	23.564079093050612	25.672397619467834	26.111568575931248	24.651954711550303
58-59	23.472404134045494	25.355716432444947	26.351049968561096	24.82082946494846
60-61	23.375137321432472	25.485822014395797	26.54979883172842	24.589241832443303
62-63	23.315144137318498	25.471727292590668	26.444735774261012	24.768392795829822
64-65	23.202843561954918	25.43656005390203	26.279637639655164	25.08095874448789
66-67	23.19505620988202	25.511872396783918	26.15391624179758	25.139155151536485
68-69	23.375957844545372	25.219293419032006	26.238632418436335	25.166116317986287
70-71	23.073539380026613	25.069873613790794	26.24614270833348	25.61044429784911
72-73	22.950053252005752	25.143391528275792	26.115368369898196	25.791186849820257
74-75	22.8513858467931	25.248599925995784	26.14443146672891	25.755582760482206
76-77	22.78553409572456	24.882953701939346	26.4859518846004	25.845560317735693
78-79	22.629990694515158	25.054031160453878	26.366609978080856	25.94936816695011
80-81	22.590987221534363	24.97391324582789	26.34515280154855	26.0899467310892
82-83	22.479995539193588	24.865491221476343	26.435924928487342	26.218588310842726
84-85	22.24799172185172	24.790800960222068	26.38814418881624	26.57306312910997
86-87	22.14835815584125	24.777951474252582	26.468246851026407	26.605443518879763
88-89	22.05640399043007	24.632087526672557	26.371166072008016	26.940342410889357
90-91	21.868760579809102	24.6448455881307	26.351384799214784	27.13500903284542
92-93	21.90990600045787	24.367613040343606	26.4800685206889	27.24241243850963
94-95	21.572325768100896	24.22035810163488	26.694871070614905	27.51244505964932
96-97	21.594054158043154	24.024097985459715	26.79161519040212	27.59023266609501
98-99	21.33269226076539	24.10988048129814	26.640395743611073	27.91703151432539
100-101	21.368530250992553	23.916804379260483	26.766660575532708	27.948004794214253
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	188.0
1	128.5
2	121.5
3	186.5
4	172.0
5	172.0
6	278.0
7	572.0
8	870.5
9	870.5
10	727.5
11	653.0
12	675.0
13	773.0
14	842.0
15	876.5
16	971.0
17	1105.5
18	1223.0
19	1347.0
20	1543.5
21	1836.0
22	2104.0
23	2454.0
24	2957.5
25	3719.0
26	4774.5
27	6196.0
28	8025.0
29	10490.0
30	14510.0
31	20976.0
32	29069.0
33	35066.0
34	39918.5
35	47348.5
36	56550.0
37	63976.0
38	72219.5
39	80899.5
40	87559.0
41	95105.5
42	104097.0
43	114550.5
44	124687.5
45	132796.0
46	140542.5
47	147673.5
48	152192.5
49	156054.0
50	158711.0
51	158905.5
52	158471.5
53	157585.0
54	153139.0
55	144378.0
56	133440.5
57	121164.5
58	110960.0
59	98334.5
60	81822.5
61	67199.0
62	54367.5
63	41446.5
64	30053.5
65	22474.5
66	16519.5
67	12215.0
68	8872.0
69	5945.0
70	4110.0
71	3026.0
72	2395.0
73	1973.5
74	1483.5
75	1134.0
76	827.5
77	439.0
78	230.0
79	131.0
80	65.0
81	27.0
82	14.0
83	6.5
84	3.5
85	3.5
86	3.0
87	0.5
88	1.0
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.18519317177141936
2	0.0
3	0.012172187940851679
4	0.013307123879392627
5	0.00700822942049036
6	0.10682584521516682
7	0.14354102282696654
8	0.12421873847330689
9	0.14257632727920672
10-11	0.11457178299570882
12-13	0.11421711551491476
14-15	0.1168132814743272
16-17	0.06561348394689862
18-19	0.07933202210401234
20-21	0.03582141556019871
22-23	0.083105684099661
24-25	0.05721495800169559
26-27	0.06479065539145643
28-29	0.038176407632671175
30-31	0.023564107423956454
32-33	0.03068583043830091
34-35	0.02032953999911475
36-37	0.02335130693548003
38-39	0.031395165399889
40-41	0.0252948847302314
42-43	0.013534111067100818
44-45	0.026146086684137114
46-47	0.03265778163151581
48-49	0.009008554012168784
50-51	0.014768353900264099
52-53	0.005958413677339983
54-55	0.012228934737778727
56-57	0.004936971332653128
58-59	0.0037452885971851315
60-61	0.021592156230741556
62-63	0.010157676649941494
64-65	0.007277776705893836
66-67	0.03921203667658979
68-69	0.09009972682091959
70-71	0.021123995156093412
72-73	0.044149008009242915
74-75	0.08531880917981584
76-77	0.23544246045031988
78-79	0.08083581222257909
80-81	0.01727939966428595
82-83	0.011391919483104775
84-85	0.012810589406280963
86-87	0.2563962152156321
88-89	0.6241722060998267
90-91	0.2689230706372779
92-93	0.3545539872001925
94-95	0.0010640024423821397
96-97	0.004256009769528559
98-99	0.0035750482064039893
100-101	0.00510721172343427
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3524428.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.235173638171
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.23196576647418	26.77093024521354
2	11.818563997974273	9.27406821228714
3	5.817452003660339	6.847462184860177
4	3.527749892501823	5.536475183373924
5	2.3058825195155266	4.523585052120746
6	1.5692313213605509	3.694143802322264
7	1.1920289288161698	3.2738623402677782
8	0.921329004188284	2.89188027657684
9	0.7008646960788638	2.474869324276635
>10	3.687899353371936	25.07336557955528
>50	0.16510181003078656	4.393112002256649
>100	0.058001802593198926	3.789945077013406
>500	0.0028375413726513484	0.7405252776577927
>1k	0.0010913620614809007	0.7157754422178161
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.8373398463523727E-5
2	0.0	0.0	0.0	0.0	2.8373398463523727E-5
3	0.0	0.0	0.0	0.0	2.8373398463523727E-5
4	0.0	0.0	0.0	0.0	2.8373398463523727E-5
5	0.0	0.0	0.0	0.0	2.8373398463523727E-5
6	0.0	0.0	0.0	0.0	8.512019539057118E-5
7	0.0	0.0	0.0	0.0	8.512019539057118E-5
8	0.0	0.0	0.0	0.0	8.512019539057118E-5
9	0.0	0.0	0.0	0.0	8.512019539057118E-5
10-11	0.0	0.0	0.0	0.0	8.512019539057118E-5
12-13	0.0	0.0	0.0	1.4186699231761863E-5	2.1280048847642795E-4
14-15	0.0	0.0	0.0	5.674679692704745E-5	2.695472854034754E-4
16-17	0.0	0.0	0.0	1.134935938540949E-4	3.12107383098761E-4
18-19	0.0	0.0	0.0	1.7024039078114236E-4	3.404807815622847E-4
20-21	0.0	0.0	0.0	1.9861378924466609E-4	3.9722757848933217E-4
22-23	0.0	0.0	0.0	4.8234777387990335E-4	4.539743754163796E-4
24-25	0.0	0.0	0.0	0.0013051763293220912	4.539743754163796E-4
26-27	0.0	0.0	0.0	0.002482672365558326	4.965344731116652E-4
28-29	0.0	0.0	0.0	0.009391594891426353	5.107211723434271E-4
30-31	0.0	0.0	0.0	0.03461554612549894	5.107211723434271E-4
32-33	0.0	0.0	0.0	0.08713470668148136	5.107211723434271E-4
34-35	0.0	0.0	0.0	0.15962873975578448	5.674679692704745E-4
36-37	0.0	0.0	0.0	0.27595967345623174	5.674679692704745E-4
38-39	0.0	0.0	0.0	0.4637490111870635	5.674679692704745E-4
40-41	0.0	0.0	0.0	0.6836002891816771	5.674679692704745E-4
42-43	0.0	0.0	0.0	0.9119352133168843	5.816546685022364E-4
44-45	0.0	0.0	0.0	1.1889305158170347	6.525881646610457E-4
46-47	0.0	0.0	0.0	1.5192394340301463	7.23521660819855E-4
48-49	0.0	0.0	0.0	1.8684024755222692	7.518950592833788E-4
50-51	0.0	0.0	0.0	2.2459672888763795	7.944551569786643E-4
52-53	0.0	0.0	0.0	2.6191909722655704	8.795753523692355E-4
54-55	0.0	0.0	0.0	3.038407934564134	9.36322149296283E-4
56-57	0.0	0.0	0.0	3.524798917725089	9.788822469915686E-4
58-59	0.0	0.0	0.0	4.045323099237663	9.930689462233303E-4
60-61	0.0	0.0	0.0	4.600051412598016	0.0010498157431503778
62-63	0.0	0.0	0.0	5.156865170745437	0.0011065625400774252
64-65	0.0	0.0	0.0	5.750606906993134	0.0011065625400774252
66-67	0.0	0.0	0.0	6.439782568972895	0.0011491226377727108
68-69	0.0	0.0	0.0	7.188556554425285	0.0011633093370044727
70-71	0.0	0.0	0.0	7.967987996917514	0.0011633093370044727
72-73	0.0	0.0	0.0	8.766443803079536	0.0012058694346997584
74-75	0.0	0.0	0.0	9.589016997935552	0.001248429532395044
76-77	0.0	0.0	0.0	10.483857238678162	0.001248429532395044
78-79	0.0	0.0	0.0	11.454610507009932	0.001248429532395044
80-81	0.0	0.0	0.0	12.456546140253113	0.0012626162316268057
82-83	0.0	0.0	0.0	13.474739730815894	0.0012768029308585676
84-85	0.0	0.0	0.0	14.495543674037318	0.0012768029308585676
86-87	0.0	0.0	0.0	15.548551424514844	0.0013051763293220912
88-89	0.0	0.0	0.0	16.67015186577794	0.001333549727785615
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	7715	0.0	40.80687	1
GGTATCA	4565	0.0	39.17042	1
ATCAACG	10015	0.0	30.905489	3
TATCAAC	10450	0.0	30.071564	2
TCAACGC	10280	0.0	30.06262	4
CAACGCA	10445	0.0	29.491793	5
AACGCAG	10800	0.0	28.52279	6
GTACATG	13215	0.0	25.910543	1
ACGCAGA	12440	0.0	24.533623	7
TACATGG	13810	0.0	24.19877	2
CGCAGAG	12645	0.0	23.871775	8
ACATGGG	14490	0.0	22.474743	3
TAAGGCG	1165	0.0	22.000511	5
TTACGTA	160	5.015634E-5	20.76907	4
GCAGAGT	13930	0.0	20.579378	9
CATGGGG	9850	0.0	19.422619	4
AGAGTAC	11605	0.0	19.179764	10-11
GTATTAA	1990	0.0	18.87927	1
GTATAGG	660	0.0	18.01388	1
GAGTACA	10215	0.0	17.458385	1
>>END_MODULE