FastQCFastQC Report
Mon 15 Aug 2016
SRR2920870_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920870_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2470505
Sequences flagged as poor quality0
Sequence length98
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA50950.042.183371
GGTATCA28050.042.0025561
TCAACGC62600.034.22314
ATCAACG63100.033.9519163
CAACGCA64300.033.3182875
TATCAAC65850.032.6043552
AACGCAG67000.032.3818446
GTACATG83350.027.2213671
ACGCAGA79700.026.1822367
TACATGG86000.025.9252742
CGCAGAG81700.025.0906968
ACATGGG88650.024.5288033
CATGGGG56400.021.961474
GCAGAGT90800.021.5150439
TAAGGCG7200.020.3889225
GAGTACA62050.018.9874551
AGAGTAC77550.018.2727510-11
CAGAGTA84900.017.52807610-11
AGTACAT63100.017.3761712
GAGTACT38100.017.33790612-13
Produced by FastQC (version 0.11.5)