##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920870_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2470505
Sequences flagged as poor quality	0
Sequence length	98
%GC	48
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.719093059920947	31.0	30.0	33.0	23.0	34.0
2	28.755181632905014	31.0	28.0	34.0	22.0	34.0
3	28.82722317906663	31.0	28.0	34.0	22.0	34.0
4	32.32441262009184	35.0	33.0	37.0	24.0	37.0
5	32.296457606845564	35.0	32.0	37.0	24.0	37.0
6	32.42525920813761	35.0	33.0	37.0	25.0	37.0
7	32.27143721627765	35.0	32.0	37.0	23.0	37.0
8	32.21464558865495	35.0	32.0	37.0	23.0	37.0
9	33.48537120953004	37.0	33.0	39.0	23.0	39.0
10-11	33.29882453182648	37.0	33.0	39.0	22.0	39.0
12-13	33.13628549628517	37.0	32.0	39.0	19.5	39.0
14-15	33.91136144229621	38.0	33.0	40.0	18.5	41.0
16-17	33.8104998775554	38.0	32.5	40.0	18.0	41.0
18-19	33.70954744070544	38.0	32.0	40.0	18.0	41.0
20-21	33.62448102715841	38.0	32.0	40.0	18.0	41.0
22-23	33.40393057289906	38.0	32.0	40.0	17.5	41.0
24-25	33.21443126000555	38.0	32.0	40.0	16.5	41.0
26-27	33.242877670759626	38.0	32.0	40.0	16.0	41.0
28-29	33.08222974654979	38.0	32.0	40.0	15.0	41.0
30-31	32.831144037352686	38.0	31.5	40.0	10.5	41.0
32-33	32.58236858456065	37.5	31.0	40.0	9.0	41.0
34-35	32.25537207979745	37.0	30.5	40.0	8.5	41.0
36-37	31.952089754928647	37.0	30.0	40.0	8.0	41.0
38-39	31.744690255635994	37.0	30.0	40.0	6.0	41.0
40-41	31.543423105802255	37.0	30.0	40.0	2.0	41.0
42-43	31.304794971068667	36.5	30.0	40.0	2.0	41.0
44-45	31.01513475989727	36.0	29.5	39.0	2.0	41.0
46-47	30.74739820401092	36.0	29.0	39.0	2.0	40.0
48-49	30.444075401587934	36.0	28.0	39.0	2.0	40.0
50-51	29.289349950718577	34.0	26.5	38.0	2.0	39.5
52-53	29.735597175476272	34.5	27.5	38.5	2.0	39.5
54-55	30.400815015553498	36.0	28.0	39.0	2.0	40.0
56-57	30.35503813997543	35.5	28.0	39.0	2.0	41.0
58-59	30.11060795262507	35.0	28.0	39.0	2.0	40.5
60-61	29.789920886620347	35.0	28.0	39.0	2.0	40.0
62-63	29.399238617205796	35.0	27.0	38.5	2.0	40.0
64-65	28.976141315237168	34.0	26.0	38.0	2.0	40.0
66-67	28.585185417556332	34.0	26.0	38.0	2.0	40.0
68-69	28.13919502287994	34.0	26.0	37.0	2.0	39.5
70-71	27.7969730884981	34.0	25.5	36.5	2.0	39.0
72-73	27.332746746110615	33.0	24.5	36.0	2.0	39.0
74-75	26.892376457444936	33.0	24.0	35.5	2.0	38.5
76-77	26.46047731131894	33.0	22.5	35.0	2.0	37.5
78-79	25.989949423296046	32.0	20.5	35.0	2.0	37.0
80-81	25.533821829949748	32.0	19.5	35.0	2.0	36.0
82-83	25.04558602391009	32.0	18.0	35.0	2.0	36.0
84-85	24.634815958680512	31.5	13.0	34.5	2.0	35.5
86-87	24.167021924667225	31.0	7.0	34.0	2.0	35.0
88-89	23.61662231001354	31.0	2.0	34.0	2.0	35.0
90-91	23.09825744129237	31.0	2.0	34.0	2.0	35.0
92-93	22.549668994800655	30.0	2.0	34.0	2.0	35.0
94-95	21.90426107212898	30.0	2.0	34.0	2.0	35.0
96-97	21.246146638035544	29.0	2.0	34.0	2.0	35.0
98	20.84408774724196	29.0	2.0	34.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	116693.0
3	19821.0
4	14662.0
5	11794.0
6	11522.0
7	11740.0
8	13455.0
9	15506.0
10	17025.0
11	18421.0
12	19191.0
13	19443.0
14	18351.0
15	19548.0
16	21388.0
17	22826.0
18	23347.0
19	22832.0
20	23721.0
21	25443.0
22	27784.0
23	31106.0
24	35564.0
25	40421.0
26	46244.0
27	53541.0
28	62646.0
29	71986.0
30	84204.0
31	98604.0
32	117213.0
33	142020.0
34	177152.0
35	224309.0
36	282025.0
37	308336.0
38	182838.0
39	17782.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.13285493985145	18.20145825513127	13.061328289402057	24.604358515615225
2	16.16453286454736	19.776058068136454	38.82185134291473	25.237557724401462
3	19.47271640804905	23.59752167562193	29.02569820279378	27.904063713535237
4	13.474028537132888	15.516969857678692	36.02395030190902	34.985051303279405
5	14.487786155699794	36.69003523909466	33.95102964229728	14.871148962908261
6	33.76338757393455	34.720462719252964	16.964793610807796	14.551356096004689
7	29.256936552963214	31.527901871109275	20.79522650344778	18.419935072479728
8	26.75782775953647	33.965960929919255	20.070688371637374	19.205522938906903
9	26.916197295694605	15.608144893452957	19.52038146047063	37.95527635038181
10-11	25.756844401619183	25.259557309043558	27.358845126572323	21.624753162764936
12-13	26.315377220057684	22.7154581794262	27.344532712644842	23.624631887871274
14-15	23.48480769939148	24.256819362143176	24.90862922998394	27.349743708481398
16-17	23.43987567743492	27.36397867830767	25.71184302778394	23.484302616473467
18-19	23.4556843082855	26.475710033635703	27.250709998204126	22.817895659874672
20-21	24.21583019997586	25.804667264576896	26.92248798710847	23.05701454833877
22-23	23.99538597019721	25.528777012666904	26.808453139215626	23.66738387792026
24-25	23.781514834423646	25.756575756403617	26.79943368328833	23.662475725884406
26-27	23.47322744142949	25.90236813723753	26.777520671200065	23.846883750132914
28-29	23.583519467659205	26.078441696249033	26.718534523783006	23.619504312308763
30-31	23.72662941804307	25.924734237557068	26.558627704171712	23.790008640228148
32-33	23.496356957811802	25.95486894805006	26.543439791324147	24.005334302813992
34-35	23.912395076948815	25.883682489715554	26.45944078533943	23.744481647996196
36-37	24.037387138749423	25.636877709798778	26.453046018225386	23.87268913322641
38-39	23.789127744248038	25.721993992294394	26.504935133368136	23.983943130089433
40-41	23.901485139758304	25.654957266677602	26.61577338093295	23.82778421263114
42-43	23.915941041190003	25.569816418735737	26.42687038438083	24.08737215569343
44-45	23.756750057901176	25.636892596369904	26.54277169871886	24.063585647010065
46-47	23.804985594917415	25.661850615809406	26.42099978851011	24.11216400076307
48-49	23.69647498949753	25.77492782345776	26.214182924163644	24.314414262881066
50-51	23.593450977500456	25.846167971916856	26.386936244353077	24.17344480622961
52-53	23.71737934774346	25.605339549094445	26.57624056751612	24.10104053564598
54-55	23.503191911439497	25.618361702266046	26.61693160236231	24.26151478393215
56-57	23.307051350955394	25.662818618152794	26.643338469438078	24.386791561453734
58-59	23.32283572389113	25.368802941362308	26.676727468284195	24.631633866462362
60-61	23.222788683684893	25.311548160174258	26.89087839004834	24.574784766092506
62-63	23.21714878702651	25.251895258380053	26.928901298665416	24.602054655928022
64-65	23.12038839608563	25.294750368196596	26.713958705882973	24.870902529834797
66-67	23.109004357799844	25.356637817961225	26.65676085119809	24.87759697304084
68-69	23.2504896196967	25.09549082558648	26.677004606356736	24.977014948360083
70-71	23.009591137743232	24.922010060702167	26.81763271288004	25.25076608867456
72-73	22.903280243543296	24.892741078476348	26.77544560149577	25.428533076484594
74-75	22.923512423475064	25.05191675776295	26.77653987905446	25.248030939707526
76-77	22.88987130103149	24.767952844217156	26.93143878576133	25.41073706899002
78-79	22.743188654850698	24.836558577405857	26.84428925517815	25.5759635125653
80-81	22.848384374393344	24.705068420714902	26.95647017828293	25.49007702660882
82-83	22.728670119425157	24.540515493350128	27.056151794666867	25.674662592557844
84-85	22.60501910047359	24.525280181905792	27.056313755813242	25.813386961807378
86-87	22.720989932245484	24.44386558893968	27.054562567963636	25.780581910851197
88-89	22.646557733461375	24.244686175199824	26.99978307603063	26.108973015308166
90-91	22.51568173644529	24.249592688104176	27.096805899001268	26.13791967644926
92-93	22.7076025149223	23.992881804987125	27.140778469328165	26.15873721076241
94-95	22.440648601054956	23.89691847081815	27.283753620622154	26.378679307504736
96-97	22.691131014097927	23.604838123411785	27.27449023810075	26.429540624389535
98	22.62645813264804	23.481925734122374	27.25922231842482	26.632393814804768
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	142.0
1	117.0
2	127.0
3	201.0
4	215.5
5	217.5
6	307.0
7	572.5
8	871.5
9	985.5
10	932.5
11	900.0
12	1029.5
13	1137.0
14	1200.5
15	1369.5
16	1511.0
17	1543.5
18	1533.0
19	1542.5
20	1639.5
21	1739.0
22	1919.5
23	2208.0
24	2595.5
25	3185.0
26	4009.5
27	5243.0
28	7036.5
29	9701.5
30	13688.0
31	18864.0
32	23745.0
33	28228.0
34	33655.5
35	40045.5
36	46536.5
37	53152.5
38	59557.0
39	65708.5
40	71887.5
41	77282.5
42	83346.0
43	89769.0
44	95106.0
45	100582.5
46	105620.0
47	108468.5
48	109999.5
49	110809.0
50	110865.0
51	110692.0
52	109377.0
53	107896.0
54	104515.5
55	99517.5
56	91740.5
57	81300.0
58	73117.0
59	63975.5
60	53074.5
61	42872.0
62	34695.0
63	27120.5
64	20117.5
65	15024.0
66	11268.5
67	8702.5
68	6431.5
69	4685.5
70	3431.0
71	2504.5
72	1950.0
73	1498.5
74	1135.0
75	809.0
76	505.5
77	309.5
78	181.0
79	114.0
80	74.0
81	41.0
82	29.0
83	20.0
84	15.0
85	10.5
86	4.5
87	3.0
88	4.5
89	5.0
90	4.5
91	4.5
92	3.0
93	3.5
94	4.0
95	2.5
96	0.5
97	0.5
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3615859915280479
2	0.023436503872689998
3	0.01817442182873542
4	0.01578624613186373
5	0.02108880573000257
6	0.023638891643611326
7	0.014814784831441346
8	0.00914792724564411
9	0.0
10-11	2.0238777092132984E-5
12-13	0.0052620820439545765
14-15	0.003076294118004214
16-17	0.006354976006929758
18-19	0.0387167805772504
20-21	0.07719069582939521
22-23	0.09635681773564514
24-25	0.06348904373802117
26-27	0.07255601587529675
28-29	0.057073351399815016
30-31	0.0502528835197662
32-33	0.01837680959965675
34-35	0.3208453332415842
36-37	0.4884021687873532
38-39	0.4451316633643729
40-41	0.4550284253624259
42-43	0.4064553603413067
44-45	0.3810961726448641
46-47	0.38026638278408664
48-49	0.37154346985737735
50-51	0.36832550429972816
52-53	0.4259048251268466
54-55	0.4265119884396105
56-57	0.397509820866584
58-59	0.3832617217937223
60-61	0.3970848065476491
62-63	0.3789913398272823
64-65	0.4267143762105318
66-67	0.4455364389062156
68-69	0.391276277522207
70-71	0.3500903661397164
72-73	0.3382102039866343
74-75	0.32013697604335956
76-77	0.31811309833414625
78-79	0.3177083227923036
80-81	0.33349456892416734
82-83	0.32892060530134526
84-85	0.32914323184935873
86-87	0.3153606246496162
88-89	0.356728685025936
90-91	0.33699587736110637
92-93	0.32718007047142184
94-95	0.3327659729488505
96-97	0.3330493158281404
98	0.3282324868802128
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
98	2470505.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.26981670353985
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.82008894407576	40.062026960340006
2	12.260436424126949	13.307432748855463
3	5.261947787100053	8.56694825728551
4	2.84250522315189	6.170489497572309
5	1.690366815053327	4.586794860734525
6	1.0240689933395644	3.334562193619004
7	0.7068886409194355	2.6853901880758513
8	0.49371359315007546	2.1434996963440516
9	0.3579621391632942	1.74838857112791
>10	1.4689091084429948	12.98646033490376
>50	0.052825955152043955	1.9207441445423512
>100	0.018937062793879703	1.8031596587609597
>500	8.245804909603528E-4	0.3011663700445693
>1k	5.247330397010568E-4	0.382936517793476
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	4.047755418426597E-5	0.0
5	0.0	0.0	0.0	4.047755418426597E-5	0.0
6	0.0	0.0	0.0	4.047755418426597E-5	0.0
7	0.0	0.0	0.0	8.095510836853194E-5	0.0
8	0.0	0.0	0.0	8.095510836853194E-5	0.0
9	0.0	0.0	0.0	8.095510836853194E-5	0.0
10-11	0.0	0.0	0.0	1.2143266255279792E-4	0.0
12-13	0.0	0.0	0.0	1.2143266255279792E-4	0.0
14-15	0.0	0.0	0.0	1.2143266255279792E-4	0.0
16-17	0.0	0.0	0.0	1.2143266255279792E-4	0.0
18-19	0.0	0.0	0.0	1.2143266255279792E-4	0.0
20-21	0.0	0.0	0.0	2.2262654801346283E-4	0.0
22-23	0.0	0.0	0.0	3.2382043347412774E-4	0.0
24-25	0.0	0.0	0.0	0.001113132740067314	0.0
26-27	0.0	0.0	0.0	0.0027524736845300863	0.0
28-29	0.0	0.0	0.0	0.010220582431527158	0.0
30-31	0.0	0.0	0.0	0.036733380422221365	4.047755418426597E-5
32-33	0.0	0.0	0.0	0.09206619699211295	4.047755418426597E-5
34-35	0.0	0.0	0.0	0.1754297198346087	8.095510836853194E-5
36-37	0.0	0.0	0.0	0.3110902426831761	8.095510836853194E-5
38-39	0.0	0.0	0.0	0.518659140540092	8.095510836853194E-5
40-41	0.0	0.0	0.0	0.7523562996229516	8.095510836853194E-5
42-43	0.0	0.0	0.0	0.9968812044501023	8.095510836853194E-5
44-45	0.0	4.047755418426597E-5	0.0	1.290404188617307	8.095510836853194E-5
46-47	0.0	4.047755418426597E-5	0.0	1.6560176967866895	8.095510836853194E-5
48-49	0.0	4.047755418426597E-5	0.0	2.040028253332821	1.0119388546066493E-4
50-51	0.0	4.047755418426597E-5	0.0	2.4501063547736193	1.2143266255279792E-4
52-53	0.0	4.047755418426597E-5	0.0	2.8595368153474694	1.2143266255279792E-4
54-55	0.0	4.047755418426597E-5	0.0	3.3084936075822555	1.2143266255279792E-4
56-57	0.0	4.047755418426597E-5	0.0	3.8477153456479547	1.2143266255279792E-4
58-59	0.0	4.047755418426597E-5	0.0	4.42551219285126	1.416714396449309E-4
60-61	0.0	4.047755418426597E-5	0.0	5.035286307860134	1.6191021673706387E-4
62-63	0.0	4.047755418426597E-5	0.0	5.6515368315384915	1.6191021673706387E-4
64-65	0.0	4.047755418426597E-5	0.0	6.316299703906692	1.6191021673706387E-4
66-67	0.0	4.047755418426597E-5	0.0	7.080293300357619	1.6191021673706387E-4
68-69	0.0	4.047755418426597E-5	0.0	7.902493619725522	1.6191021673706387E-4
70-71	0.0	4.047755418426597E-5	0.0	8.762439258370252	1.6191021673706387E-4
72-73	0.0	4.047755418426597E-5	0.0	9.631512585483534	1.8214899382919687E-4
74-75	0.0	4.047755418426597E-5	0.0	10.533210821269336	2.0238777092132984E-4
76-77	0.0	4.047755418426597E-5	0.0	11.514386734695943	2.0238777092132984E-4
78-79	0.0	4.047755418426597E-5	0.0	12.587466934897925	2.0238777092132984E-4
80-81	0.0	4.047755418426597E-5	0.0	13.692989894778599	2.0238777092132984E-4
82-83	0.0	4.047755418426597E-5	0.0	14.814602682447516	2.0238777092132984E-4
84-85	0.0	4.047755418426597E-5	0.0	15.950180226310005	2.2262654801346283E-4
86	0.0	4.047755418426597E-5	0.0	16.818545196225063	2.4286532510559583E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	5095	0.0	42.18337	1
GGTATCA	2805	0.0	42.002556	1
TCAACGC	6260	0.0	34.2231	4
ATCAACG	6310	0.0	33.951916	3
CAACGCA	6430	0.0	33.318287	5
TATCAAC	6585	0.0	32.604355	2
AACGCAG	6700	0.0	32.381844	6
GTACATG	8335	0.0	27.221367	1
ACGCAGA	7970	0.0	26.182236	7
TACATGG	8600	0.0	25.925274	2
CGCAGAG	8170	0.0	25.090696	8
ACATGGG	8865	0.0	24.528803	3
CATGGGG	5640	0.0	21.96147	4
GCAGAGT	9080	0.0	21.515043	9
TAAGGCG	720	0.0	20.388922	5
GAGTACA	6205	0.0	18.987455	1
AGAGTAC	7755	0.0	18.27275	10-11
CAGAGTA	8490	0.0	17.528076	10-11
AGTACAT	6310	0.0	17.376171	2
GAGTACT	3810	0.0	17.337906	12-13
>>END_MODULE