FastQCFastQC Report
Mon 15 Aug 2016
SRR2920870_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920870_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2470505
Sequences flagged as poor quality0
Sequence length101
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA27800.047.5547871
GTATCAA50450.042.3232841
ATCAACG65450.032.1994173
TCAACGC65650.032.0290264
TATCAAC66650.031.6896132
CAACGCA66850.031.1669125
AACGCAG67700.030.915816
GTACATG81100.026.0935631
ACGCAGA81400.025.0711787
TACATGG83650.024.228082
CGCAGAG84450.023.884718
ACATGGG85700.022.9848653
TAAGGCG7500.020.8825445
GCAGAGT92200.020.5897459
CATGGGG56050.019.3078524
GTGGTAT16950.019.0780241
AGAGTAC79200.018.33471310-11
CAGAGTA84200.017.72500810-11
TAATACT14350.017.199914
TAAGGTG10600.017.0140785
Produced by FastQC (version 0.11.5)