##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920870_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2470505
Sequences flagged as poor quality	0
Sequence length	101
%GC	48
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.08097048983912	31.0	30.0	34.0	26.0	34.0
2	30.09659482575425	31.0	30.0	34.0	26.0	34.0
3	30.23254597744186	31.0	30.0	34.0	26.0	34.0
4	33.87415649836774	35.0	35.0	37.0	30.0	37.0
5	33.37296585111141	35.0	33.0	37.0	27.0	37.0
6	33.31631872835716	35.0	33.0	37.0	27.0	37.0
7	33.1270298987454	35.0	33.0	37.0	26.0	37.0
8	33.05301264316405	35.0	33.0	37.0	26.0	37.0
9	34.3597697636718	37.0	34.0	39.0	27.0	39.0
10-11	34.156396769081624	37.0	33.5	39.0	26.0	39.0
12-13	33.97536799156448	37.0	33.0	39.0	25.0	39.0
14-15	34.83589630460169	38.0	33.5	40.0	25.0	41.0
16-17	34.70698379481118	38.0	33.0	40.0	25.0	41.0
18-19	34.615032958848495	38.0	33.5	40.0	25.0	41.0
20-21	34.50203278277114	38.0	33.0	40.0	24.0	41.0
22-23	34.347842647555865	38.0	33.0	40.0	23.5	41.0
24-25	34.15911605117172	38.0	33.0	40.0	22.0	41.0
26-27	33.967343923610755	38.0	33.0	40.0	21.0	41.0
28-29	33.906553518410206	38.0	32.5	40.0	20.5	41.0
30-31	33.87608950396781	38.0	33.0	40.0	20.0	41.0
32-33	33.70916047528744	38.0	32.5	40.0	19.0	41.0
34-35	33.52603617479018	38.0	32.0	40.0	18.0	41.0
36-37	33.324026666612696	38.0	32.0	40.0	17.0	41.0
38-39	33.126905835041825	38.0	32.0	40.0	16.0	41.0
40-41	32.8805982987284	37.5	31.0	40.0	15.0	41.0
42-43	32.891791759174744	38.0	31.5	40.0	14.5	41.0
44-45	32.73237273350995	37.5	31.0	40.0	11.0	41.0
46-47	32.46316036599805	37.0	31.0	40.0	9.0	41.0
48-49	32.19069542461966	37.0	31.0	40.0	8.0	41.0
50-51	31.918109050578728	37.0	30.0	40.0	7.0	41.0
52-53	31.645556272907765	36.0	30.0	40.0	3.5	41.0
54-55	31.354527920404937	36.0	30.0	39.0	2.0	41.0
56-57	31.018974663074957	35.5	29.0	39.0	2.0	40.5
58-59	30.67630707082155	35.0	29.0	39.0	2.0	40.0
60-61	30.33835470885507	35.0	28.0	39.0	2.0	40.0
62-63	30.005042086536964	35.0	28.0	38.5	2.0	40.0
64-65	29.592093721728958	34.0	28.0	38.0	2.0	40.0
66-67	29.150151284858765	34.0	27.0	37.5	2.0	40.0
68-69	28.70555109178083	34.0	26.0	37.0	2.0	39.0
70-71	28.278116012718048	34.0	26.0	36.0	2.0	39.0
72-73	27.84285075318609	33.0	26.0	36.0	2.0	39.0
74-75	27.405047551006778	33.0	25.0	35.5	2.0	38.0
76-77	25.98299295083394	30.5	23.0	34.0	2.0	36.0
78-79	26.82628389742178	32.5	25.0	35.0	2.0	37.0
80-81	26.86506503731019	33.0	25.0	35.0	2.0	36.5
82-83	26.534072183622378	33.0	24.5	35.0	2.0	36.0
84-85	26.217428218117348	32.5	24.0	35.0	2.0	36.0
86-87	25.680487390229935	32.0	21.5	35.0	2.0	35.0
88-89	25.322721063102485	32.0	20.0	34.5	2.0	35.0
90-91	24.962426912716225	32.0	18.5	34.0	2.0	35.0
92-93	24.652518412227458	32.0	17.5	34.0	2.0	35.0
94-95	24.27252201472978	31.5	9.5	34.0	2.0	35.0
96-97	23.737679138475738	31.0	2.0	34.0	2.0	35.0
98-99	23.132267491869072	31.0	2.0	34.0	2.0	35.0
100-101	20.976623605295273	28.0	2.0	33.0	2.0	34.5
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	62469.0
3	28406.0
4	15134.0
5	10704.0
6	9320.0
7	9669.0
8	10738.0
9	12307.0
10	13916.0
11	15688.0
12	17058.0
13	18746.0
14	20340.0
15	21615.0
16	23246.0
17	24106.0
18	22855.0
19	21766.0
20	21756.0
21	22261.0
22	24534.0
23	27510.0
24	32339.0
25	37968.0
26	44202.0
27	52118.0
28	61523.0
29	72027.0
30	85379.0
31	100311.0
32	120363.0
33	146740.0
34	182939.0
35	233633.0
36	305605.0
37	344163.0
38	185616.0
39	11435.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.09225199095826	19.64144502327347	13.780943088481356	24.485359897286912
2	16.297194298331718	20.8664625248684	37.61510298501724	25.221240191782652
3	19.255292275455794	24.49336168186919	29.36138067949044	26.889965363184583
4	13.876396511499397	16.605314688485382	35.461038558837785	34.05725024117744
5	14.469384260591731	36.990916660695845	33.92203605404423	14.617663024668195
6	33.63310176040002	35.300467400487456	16.65558439243002	14.410846446682509
7	29.09412163852015	31.6871575590254	20.707846800465823	18.510874001988626
8	26.796791222793114	34.40162886699883	19.91564680107089	18.885933109137166
9	26.580709440986183	15.548464739742872	19.473125902650178	38.39769991662076
10-11	25.751533154989524	25.49031884072066	27.31505035750822	21.443097646781595
12-13	26.28018683525848	22.808481703164706	27.392319800886277	23.519011660690538
14-15	23.437589911877243	24.399054339800642	24.78593375029481	27.37742199802731
16-17	23.434972599907205	27.553077237296204	25.631516496255287	23.380433666541308
18-19	23.54377215708284	26.49457961014674	27.153901884695163	22.807746348075252
20-21	24.261582146816014	25.79969326861267	26.849200524379068	23.089524060192236
22-23	24.02915492524108	25.68609356086447	26.698725687349402	23.586025826545047
24-25	23.753003079624744	25.833491283576578	26.795550015147025	23.617955621651657
26-27	23.45840551398769	25.966079919122564	26.839902937356037	23.73561162953371
28-29	23.57189656024173	26.050562575773405	26.83079074837601	23.546750115608855
30-31	23.75680922076137	25.909200133041583	26.565217400105958	23.768773246091094
32-33	23.60179437417779	25.94434832464746	26.54832391863204	23.90553338254271
34-35	23.883599856440068	25.988437069150667	26.5152485872241	23.612714487185173
36-37	23.980962734208067	25.695855183980683	26.443458571577512	23.879723510233745
38-39	23.811115475096265	25.728951982216536	26.554271554150084	23.905660988537118
40-41	23.86255906421194	25.710668412233733	26.66685021168071	23.759922311873616
42-43	23.89563061596931	25.559451345403673	26.53259589958335	24.01232213904367
44-45	23.718139090266984	25.62369514180277	26.626648147372123	24.03151762055812
46-47	23.787335623647724	25.634562111500074	26.45396125928285	24.124141005569356
48-49	23.630844226620308	25.766584942135346	26.301805394952538	24.300765436291805
50-51	23.557907474269115	25.779163037478327	26.42675935257777	24.236170135674797
52-53	23.70756029158881	25.629349624827146	26.523198538817518	24.139891544766527
54-55	23.50746789792675	25.59626087940421	26.563123366406817	24.33314785626222
56-57	23.38293766248749	25.728869921965526	26.394880145078748	24.493312270468238
58-59	23.236456575875234	25.622990223540576	26.469160759448577	24.671392441135612
60-61	23.202107898627826	25.629324989837844	26.539182201406668	24.629384910127662
62-63	23.083563605803757	25.616756441219596	26.58331383472684	24.716366118249805
64-65	23.021717134610125	25.595279659948332	26.394848786427566	24.988154419013973
66-67	22.889114707059637	25.65612967767055	26.328826215910382	25.125929399359435
68-69	23.14319519819051	25.423694136840876	26.293476188961762	25.139634476006854
70-71	22.87736512686244	25.215118269248094	26.391444297996923	25.51607230589254
72-73	22.772651703989	25.19525053194925	26.3537852777667	25.678312486295056
74-75	22.731728086698148	25.30310300914368	26.25182479607961	25.713344108078566
76-77	22.727202647282688	25.049376885612784	26.526626191415158	25.69679427568937
78-79	22.615421728818866	25.131601947479943	26.34570827460221	25.907268049098985
80-81	22.57011931852455	25.04140502187958	26.406485376568096	25.981990283027766
82-83	22.426145092633636	24.891841398420787	26.472331355772678	26.209682153172903
84-85	22.291272087573635	24.802166468200593	26.421350051038473	26.485211393187303
86-87	22.314179040730476	24.781998494071548	26.466621528040562	26.437200937157414
88-89	22.176709620824553	24.7046205924204	26.280988773709158	26.837681013045888
90-91	21.898770317740123	24.645126309323764	26.432375186712882	27.023728186223234
92-93	21.970676599954384	24.460836093238104	26.456294461512403	27.11219284529511
94-95	21.723682761836606	24.30260290341279	26.594634547568237	27.37907978718237
96-97	21.845862394196867	24.070146818923327	26.603046482539195	27.48094430434061
98-99	21.64166311294598	24.154236402742775	26.512463186860675	27.691637297450573
100-101	21.735937821623548	23.86575487582561	26.634765996195313	27.763541306355528
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	127.0
1	93.5
2	100.5
3	139.5
4	127.0
5	138.5
6	221.5
7	426.5
8	661.0
9	718.5
10	567.5
11	448.5
12	461.5
13	482.0
14	539.0
15	605.5
16	666.0
17	750.0
18	801.0
19	879.0
20	1007.5
21	1209.0
22	1445.0
23	1700.5
24	2031.0
25	2474.0
26	3223.0
27	4294.5
28	5649.0
29	7675.0
30	10634.5
31	14973.0
32	20364.0
33	24683.0
34	28508.0
35	34077.0
36	40963.0
37	47152.5
38	53114.0
39	59092.0
40	64968.0
41	71293.5
42	76976.5
43	83197.5
44	88983.5
45	94268.0
46	100138.0
47	104657.0
48	107910.5
49	109724.5
50	110209.5
51	110542.5
52	110049.5
53	108797.0
54	105475.5
55	99568.5
56	91972.0
57	82119.0
58	73337.0
59	64497.0
60	53644.5
61	43671.5
62	35106.0
63	27100.0
64	20163.0
65	15103.5
66	11547.5
67	8841.5
68	6458.0
69	4640.0
70	3355.0
71	2431.5
72	1880.0
73	1512.0
74	1121.5
75	811.0
76	543.5
77	322.5
78	183.5
79	91.0
80	53.0
81	32.0
82	20.0
83	13.0
84	3.5
85	2.5
86	3.0
87	1.0
88	1.0
89	1.5
90	1.0
91	1.0
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.5
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.18648009212691333
2	0.0
3	0.012183743809464057
4	0.013438547989176302
5	0.006395453561114023
6	0.10524164087909152
7	0.14061902323613998
8	0.12236364629903602
9	0.14021424769429733
10-11	0.11331691293885257
12-13	0.11210258631332459
14-15	0.11378240481197165
16-17	0.0658367418807086
18-19	0.07729188971485587
20-21	0.03713815596406402
22-23	0.08245277787334979
24-25	0.055575681894997175
26-27	0.06411644582787729
28-29	0.039303705112922256
30-31	0.024306771287651713
32-33	0.031815357588833054
34-35	0.018478003485117415
36-37	0.024792501937862903
38-39	0.032179655576491446
40-41	0.02548062035899543
42-43	0.012831384676412313
44-45	0.026249693888496482
46-47	0.033576131195848624
48-49	0.00868243537252505
50-51	0.014996933825270543
52-53	0.007164527090615077
54-55	0.012082549924003393
56-57	0.004958500387572581
58-59	0.0032786818889255437
60-61	0.022545997680636146
62-63	0.00983604566677663
64-65	0.0070633332051544115
66-67	0.03912155611909306
68-69	0.08846369466971328
70-71	0.021311432278016032
72-73	0.042481193116387136
74-75	0.08413259637199681
76-77	0.23432456117271572
78-79	0.08020627361612301
80-81	0.015948156348600792
82-83	0.011738490713437131
84-85	0.012527803020030318
86-87	0.25276208710364884
88-89	0.6079323862934906
90-91	0.2628814756497153
92-93	0.3486938905203592
94-95	0.001335759288080777
96-97	0.0046549187311905865
98-99	0.003622741099491804
100-101	0.005201365712678177
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2470505.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.45592902623456
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.40208809165405	37.97918794286987
2	12.75671328208476	13.383304930661241
3	5.6001822332655475	8.81288285286472
4	2.975923695804167	6.244193686983721
5	1.783769959887953	4.678465520750586
6	1.149672336110903	3.618427827987529
7	0.760735201772762	2.7933550226365162
8	0.5112230115242083	2.145334240727341
9	0.3809345890636934	1.7984049990806632
>10	1.5973707552555858	13.671969404987227
>50	0.05951226661903055	2.124788196154892
>100	0.02009446031966621	1.859999155060978
>500	0.0011609456332377951	0.412282721381883
>1k	6.1917100438783E-4	0.47740349785284797
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	4.047755418426597E-5	0.0
5	0.0	0.0	0.0	4.047755418426597E-5	0.0
6	0.0	0.0	0.0	4.047755418426597E-5	0.0
7	0.0	0.0	0.0	8.095510836853194E-5	0.0
8	0.0	0.0	0.0	8.095510836853194E-5	0.0
9	0.0	0.0	0.0	8.095510836853194E-5	0.0
10-11	0.0	0.0	0.0	1.2143266255279792E-4	0.0
12-13	0.0	0.0	0.0	1.2143266255279792E-4	6.071633127639895E-5
14-15	0.0	0.0	0.0	1.2143266255279792E-4	1.0119388546066493E-4
16-17	0.0	0.0	0.0	1.2143266255279792E-4	1.2143266255279792E-4
18-19	0.0	0.0	0.0	1.2143266255279792E-4	1.2143266255279792E-4
20-21	0.0	0.0	0.0	2.428653251055958E-4	1.2143266255279792E-4
22-23	0.0	4.047755418426597E-5	0.0	3.6429798765839373E-4	1.2143266255279792E-4
24-25	0.0	4.047755418426597E-5	0.0	0.001092893962975181	1.2143266255279792E-4
26-27	0.0	4.047755418426597E-5	0.0	0.0028131900158064847	1.6191021673706387E-4
28-29	0.0	4.047755418426597E-5	0.0	0.010159866100250759	1.6191021673706387E-4
30-31	0.0	4.047755418426597E-5	0.0	0.03679409675349776	1.6191021673706387E-4
32-33	0.0	8.095510836853194E-5	0.0	0.09210667454629722	1.6191021673706387E-4
34-35	2.0238777092132984E-5	8.095510836853194E-5	0.0	0.17565234638262217	1.6191021673706387E-4
36-37	4.047755418426597E-5	8.095510836853194E-5	0.0	0.31074618347260985	1.6191021673706387E-4
38-39	4.047755418426597E-5	8.095510836853194E-5	0.0	0.5193067814070402	2.0238777092132984E-4
40-41	4.047755418426597E-5	8.095510836853194E-5	0.0	0.7515669873163584	2.0238777092132984E-4
42-43	4.047755418426597E-5	8.095510836853194E-5	0.0	0.9964764289082597	2.0238777092132984E-4
44-45	4.047755418426597E-5	8.095510836853194E-5	0.0	1.2902018008463858	2.0238777092132984E-4
46-47	4.047755418426597E-5	8.095510836853194E-5	0.0	1.6556736375761232	2.0238777092132984E-4
48-49	4.047755418426597E-5	8.095510836853194E-5	0.0	2.0366281387813423	2.0238777092132984E-4
50-51	4.047755418426597E-5	8.095510836853194E-5	0.0	2.4401083988901053	2.0238777092132984E-4
52-53	4.047755418426597E-5	8.095510836853194E-5	0.0	2.8414231098500102	2.4286532510559583E-4
54-55	4.047755418426597E-5	8.095510836853194E-5	0.0	3.284166597517512	2.4286532510559583E-4
56-57	4.047755418426597E-5	8.095510836853194E-5	0.0	3.8168309718053597	2.4286532510559583E-4
58-59	4.047755418426597E-5	8.095510836853194E-5	0.0	4.387787112351523	2.4286532510559583E-4
60-61	4.047755418426597E-5	8.095510836853194E-5	0.0	4.992622965749917	2.631041021977288E-4
62-63	4.047755418426597E-5	8.095510836853194E-5	4.047755418426597E-5	5.6021946929878705	3.2382043347412774E-4
64-65	4.047755418426597E-5	8.095510836853194E-5	4.047755418426597E-5	6.2556238501844765	3.2382043347412774E-4
66-67	4.047755418426597E-5	8.095510836853194E-5	4.047755418426597E-5	7.006543196633887	3.2382043347412774E-4
68-69	4.047755418426597E-5	8.095510836853194E-5	4.047755418426597E-5	7.8178550539262215	3.2382043347412774E-4
70-71	4.047755418426597E-5	8.095510836853194E-5	4.047755418426597E-5	8.66213587910164	3.2382043347412774E-4
72-73	4.047755418426597E-5	8.095510836853194E-5	4.047755418426597E-5	9.518802835857446	3.4405921056626074E-4
74-75	4.047755418426597E-5	8.095510836853194E-5	4.047755418426597E-5	10.416392599893545	3.6429798765839373E-4
76-77	4.047755418426597E-5	8.095510836853194E-5	4.047755418426597E-5	11.391517118969603	3.6429798765839373E-4
78-79	4.047755418426597E-5	8.095510836853194E-5	4.047755418426597E-5	12.458991177917067	3.6429798765839373E-4
80-81	4.047755418426597E-5	8.095510836853194E-5	4.047755418426597E-5	13.553666153276355	3.6429798765839373E-4
82-83	6.071633127639895E-5	8.095510836853194E-5	4.047755418426597E-5	14.66463334419481	4.047755418426597E-4
84-85	8.095510836853194E-5	8.095510836853194E-5	4.047755418426597E-5	15.792843973195763	4.047755418426597E-4
86-87	8.095510836853194E-5	8.095510836853194E-5	4.047755418426597E-5	16.9532544965503	4.047755418426597E-4
88-89	8.095510836853194E-5	8.095510836853194E-5	4.047755418426597E-5	18.169827626335504	4.047755418426597E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2780	0.0	47.554787	1
GTATCAA	5045	0.0	42.323284	1
ATCAACG	6545	0.0	32.199417	3
TCAACGC	6565	0.0	32.029026	4
TATCAAC	6665	0.0	31.689613	2
CAACGCA	6685	0.0	31.166912	5
AACGCAG	6770	0.0	30.91581	6
GTACATG	8110	0.0	26.093563	1
ACGCAGA	8140	0.0	25.071178	7
TACATGG	8365	0.0	24.22808	2
CGCAGAG	8445	0.0	23.88471	8
ACATGGG	8570	0.0	22.984865	3
TAAGGCG	750	0.0	20.882544	5
GCAGAGT	9220	0.0	20.589745	9
CATGGGG	5605	0.0	19.307852	4
GTGGTAT	1695	0.0	19.078024	1
AGAGTAC	7920	0.0	18.334713	10-11
CAGAGTA	8420	0.0	17.725008	10-11
TAATACT	1435	0.0	17.19991	4
TAAGGTG	1060	0.0	17.014078	5
>>END_MODULE