FastQCFastQC Report
Mon 15 Aug 2016
SRR2920869_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920869_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2055597
Sequences flagged as poor quality0
Sequence length98
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26750.040.7775651
GTATCAA45400.036.293091
TCAACGC55850.028.911874
ATCAACG56000.028.7525123
TATCAAC57050.028.4645482
CAACGCA57400.027.8913965
AACGCAG60000.026.6788716
GTACATG73900.023.7911641
ACGCAGA68850.023.0446627
TACATGG75850.022.80042
CGCAGAG70200.022.5334288
ACATGGG75500.021.6301613
GTATAGG5150.021.4486891
GTATTAG5400.019.6033741
GACCGCG2853.0468073E-919.3078787
CATGGGG50000.019.0831474
GCAGAGT78200.018.9960129
GAGTACA54500.018.3256761
AGAGTAC60700.017.60106510-11
GTATATA6650.017.302751
Produced by FastQC (version 0.11.5)