##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920869_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2055597
Sequences flagged as poor quality	0
Sequence length	98
%GC	48
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.62567030405279	31.0	30.0	33.0	22.0	34.0
2	28.661899195221633	31.0	28.0	34.0	18.0	34.0
3	28.73023360123604	31.0	28.0	34.0	22.0	34.0
4	32.214470540675045	35.0	32.0	37.0	23.0	37.0
5	32.180740193724745	35.0	32.0	37.0	22.0	37.0
6	32.3046224527473	35.0	32.0	37.0	24.0	37.0
7	32.13314866678634	35.0	32.0	37.0	22.0	37.0
8	32.08005654804906	35.0	32.0	37.0	22.0	37.0
9	33.33911608160549	37.0	33.0	39.0	22.0	39.0
10-11	33.14355780826689	37.0	32.0	39.0	19.5	39.0
12-13	32.97754423654053	37.0	32.0	39.0	18.0	39.0
14-15	33.73941219995943	38.0	32.0	40.0	17.5	41.0
16-17	33.62685803686229	38.0	32.0	40.0	17.0	41.0
18-19	33.518035879600916	38.0	32.0	40.0	17.5	41.0
20-21	33.42753078546038	38.0	32.0	40.0	18.0	41.0
22-23	33.204348420434556	38.0	32.0	40.0	16.0	41.0
24-25	33.01187830104831	38.0	32.0	40.0	15.5	41.0
26-27	33.047798279526575	38.0	32.0	40.0	13.0	41.0
28-29	32.887701966873855	38.0	32.0	40.0	10.0	41.0
30-31	32.63588144952537	38.0	31.0	40.0	9.5	41.0
32-33	32.392426628371226	37.0	31.0	40.0	9.0	41.0
34-35	32.06808143814182	37.0	30.0	40.0	8.0	41.0
36-37	31.76455185525178	37.0	30.0	40.0	4.5	41.0
38-39	31.564782639787857	37.0	30.0	40.0	2.0	41.0
40-41	31.3615976283289	37.0	30.0	40.0	2.0	41.0
42-43	31.124150307672174	36.5	30.0	40.0	2.0	41.0
44-45	30.839385103208457	36.0	29.0	39.0	2.0	41.0
46-47	30.57698493430376	36.0	28.5	39.0	2.0	40.0
48-49	30.269825262442005	35.5	28.0	39.0	2.0	40.0
50-51	29.12276093027962	34.0	25.5	38.0	2.0	39.5
52-53	29.57361802921487	34.5	27.5	38.5	2.0	39.5
54-55	30.21753169517177	36.0	28.0	39.0	2.0	40.0
56-57	30.173057267548067	35.5	28.0	39.0	2.0	41.0
58-59	29.922373889434553	35.0	28.0	39.0	2.0	40.5
60-61	29.595890390966712	35.0	28.0	39.0	2.0	40.0
62-63	29.199755107640264	35.0	26.5	38.5	2.0	40.0
64-65	28.773801236331828	34.0	26.0	38.0	2.0	40.0
66-67	28.37962207572788	34.0	26.0	37.5	2.0	40.0
68-69	27.92551531258316	34.0	25.5	37.0	2.0	39.5
70-71	27.59002348222925	34.0	25.0	36.5	2.0	39.0
72-73	27.120857347038353	33.0	24.0	36.0	2.0	39.0
74-75	26.66828517457459	33.0	22.5	35.5	2.0	38.5
76-77	26.24734079685853	33.0	21.5	35.0	2.0	37.5
78-79	25.783510581110985	32.0	20.0	35.0	2.0	37.0
80-81	25.329170552399134	32.0	18.5	35.0	2.0	36.0
82-83	24.853489035058914	32.0	16.0	35.0	2.0	36.0
84-85	24.441575367156112	31.0	9.0	34.5	2.0	35.5
86-87	23.979319876415467	31.0	4.5	34.0	2.0	35.0
88-89	23.44461803553907	31.0	2.0	34.0	2.0	35.0
90-91	22.93911963288524	31.0	2.0	34.0	2.0	35.0
92-93	22.39851074894544	30.0	2.0	34.0	2.0	35.0
94-95	21.754108173927087	30.0	2.0	34.0	2.0	35.0
96-97	21.101837568356054	29.0	2.0	34.0	2.0	35.0
98	20.702059790902595	29.0	2.0	34.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	102486.0
3	17622.0
4	13068.0
5	10579.0
6	9883.0
7	10035.0
8	11534.0
9	13258.0
10	14644.0
11	15771.0
12	16643.0
13	16447.0
14	15626.0
15	16449.0
16	17822.0
17	19087.0
18	19613.0
19	19315.0
20	20180.0
21	21206.0
22	23384.0
23	26027.0
24	29659.0
25	33492.0
26	38852.0
27	44541.0
28	51567.0
29	60066.0
30	69626.0
31	81439.0
32	96441.0
33	116589.0
34	144328.0
35	183311.0
36	232207.0
37	256194.0
38	151205.0
39	15400.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.24250769408804	17.87889752006957	13.028655747726686	24.849939038115703
2	15.969360844085195	19.566230753210963	39.15002715132925	25.314381251374595
3	19.47265808409776	23.648936309968953	28.806846463035413	28.071559142897872
4	13.378270746454415	15.57463790010524	36.22088811236105	34.8262032410793
5	14.54924802902298	36.7170283753544	33.95413290878402	14.779590686838597
6	33.664794291786855	34.78228289599172	17.079387137835596	14.473535674385834
7	29.36253464221212	31.41133929127483	20.701251790496357	18.52487427601669
8	26.94139236909808	34.21971634186877	19.869788930015687	18.969102359017473
9	27.116550569007448	15.645722386245941	19.283497689479017	37.95422935526759
10-11	25.76620740802361	25.198464386597458	27.491692796564305	21.543635408814623
12-13	26.367727403198266	22.567417014921016	27.40217660995675	23.66267897192397
14-15	23.648553815452416	24.135435662684483	24.849242007959507	27.366768513903594
16-17	23.577038995662825	27.21365030637538	25.59104055733805	23.618270140623746
18-19	23.640823119766992	26.351098490655104	27.022188805590634	22.98588958398727
20-21	24.522237409187557	25.674193748623132	26.68004688350083	23.123521958688478
22-23	24.259596960526284	25.402613402024205	26.5244723406431	23.81331729680641
24-25	23.845429850068406	25.727868350513933	26.545663773634224	23.881038025783436
26-27	23.732185015148715	25.88077849819474	26.608092118602748	23.778944368053796
28-29	23.766302005365393	25.905984701115404	26.565451272315133	23.762262021204076
30-31	24.039082113518024	25.92280727713081	26.26709258395772	23.771018025393445
32-33	23.8047705297115	25.904786464725838	26.304694503879748	23.985748501682906
34-35	24.082659870912888	25.837871685489937	26.34137925396543	23.738089189631744
36-37	24.12083432643358	25.599129738616018	26.307586063595085	23.972449871355323
38-39	23.928183538558713	25.69117113463021	26.397582987082956	23.98306233972812
40-41	24.060956972267718	25.658704686332385	26.428975333060617	23.851363008339284
42-43	24.025735805958547	25.467012259728232	26.337874534282246	24.16937740003097
44-45	23.88071619122037	25.69231614482544	26.357082247223936	24.069885416730255
46-47	24.025431913007193	25.527631859433935	26.300805772042136	24.14613045551674
48-49	23.782216417759216	25.66848784542028	26.213742757951696	24.335552978868808
50-51	23.564529226526272	25.871531902904266	26.265981482647977	24.297957387921485
52-53	23.841179646409003	25.664003048787936	26.31458214749	24.18023515731306
54-55	23.66283967072656	25.561736815772605	26.47697157554058	24.298451937960255
56-57	23.682862151546992	25.615066485159225	26.369426253810506	24.332645109483273
58-59	23.548866576918464	25.389624486070268	26.48948356364988	24.572025373361388
60-61	23.541486550513117	25.348143155097713	26.65169565620894	24.458674638180227
62-63	23.354462833729713	25.37442190956639	26.671542079123306	24.59957317758059
64-65	23.400953683166996	25.30201717743691	26.542186396468654	24.754842742927444
66-67	23.376717307774538	25.395690924307118	26.45581884044691	24.771772927471428
68-69	23.523157240267082	25.083481525148976	26.499651519273403	24.89370971531054
70-71	23.2537049870709	24.92949594697812	26.659210027503477	25.157589038447504
72-73	23.143915726168625	24.94010426836412	26.639371070410434	25.27660893505682
74-75	23.108230255040684	25.036897343391274	26.585341212575553	25.269531188992488
76-77	23.057834561153445	24.876296788145574	26.803448487071346	25.262420163629635
78-79	23.078319647628692	24.86470064300443	26.648153344965163	25.408826364401715
80-81	22.999355670103093	24.825249921899406	26.632668502030615	25.542725905966883
82-83	22.92363441830134	24.67692085415117	26.79926627500825	25.60017845253924
84-85	22.9085433488346	24.634443070983266	26.786942817781025	25.670070762401114
86-87	22.817934270032104	24.59740815600942	26.909823770518805	25.67483380343967
88-89	22.890884780740098	24.349956533977103	26.871254396952573	25.887904288330233
90-91	22.792562947855842	24.25650346666875	26.95102779705264	25.999905788422762
92-93	22.87913013683252	24.145955987508678	26.98337353608144	25.99154033957737
94-95	22.855792128347268	23.993796828562704	27.090476828533415	26.059934214556606
96-97	22.858683260532594	23.836361741526098	27.050048361623396	26.25490663631791
98	22.918118790037116	23.700557029643818	26.944722666401134	26.43660151391793
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	99.0
1	72.5
2	85.0
3	126.0
4	121.5
5	128.5
6	213.0
7	425.0
8	631.0
9	732.0
10	726.0
11	715.5
12	811.0
13	918.5
14	1003.0
15	1127.0
16	1216.5
17	1197.0
18	1236.0
19	1321.0
20	1330.5
21	1369.0
22	1508.5
23	1674.5
24	1932.5
25	2378.0
26	3106.0
27	4211.5
28	5626.5
29	7678.0
30	10642.5
31	14550.5
32	18574.5
33	22510.0
34	27218.5
35	33188.0
36	39807.5
37	45634.5
38	50458.0
39	54612.5
40	59114.0
41	62971.5
42	67026.0
43	73156.5
44	78310.0
45	81981.5
46	86081.0
47	89306.5
48	91725.5
49	92463.5
50	91790.0
51	91623.0
52	90366.0
53	89106.5
54	86971.0
55	83410.0
56	77797.0
57	70459.0
58	63383.0
59	55529.5
60	46743.0
61	38339.0
62	30848.5
63	23223.5
64	16940.5
65	12735.5
66	9572.5
67	7221.0
68	5147.5
69	3542.0
70	2605.0
71	1886.5
72	1428.5
73	1130.5
74	875.5
75	634.5
76	444.5
77	261.0
78	127.0
79	70.0
80	48.5
81	33.0
82	17.0
83	10.5
84	9.0
85	8.5
86	8.0
87	6.5
88	6.0
89	3.5
90	4.0
91	4.0
92	2.0
93	4.5
94	5.0
95	2.0
96	0.0
97	0.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.36952768465803365
2	0.021842802845110205
3	0.016832093061042608
4	0.014302414335105566
5	0.019361771786979648
6	0.02203739351633613
7	0.014642948009750939
8	0.008026865188069452
9	0.0
10-11	7.297150170972229E-5
12-13	0.005205300455293523
14-15	0.003283717576937503
16-17	0.005448538794325931
18-19	0.03561009283434447
20-21	0.07635251462227276
22-23	0.09496024755825193
24-25	0.06292575830768385
26-27	0.07114721416697924
28-29	0.055142131458646805
30-31	0.051128698864612084
32-33	0.01821855159352733
34-35	0.324504268103135
36-37	0.49788455616543514
38-39	0.4509395567321805
40-41	0.4620555488259615
42-43	0.41292140434141517
44-45	0.3872111119056897
46-47	0.3859462725427211
48-49	0.3768978063307156
50-51	0.3736140887537781
52-53	0.4320885854571689
54-55	0.4334020724879439
56-57	0.4036783474581837
58-59	0.38998402897065915
60-61	0.40411617646844206
62-63	0.3846571093458494
64-65	0.4329885673115888
66-67	0.45410165513960177
68-69	0.39589472060914666
70-71	0.3556387754992832
72-73	0.3423336383542105
74-75	0.3244312966014253
76-77	0.3230448380689406
78-79	0.3221448562145206
80-81	0.33844182492969194
82-83	0.3343554208339475
84-85	0.3327257239624304
86-87	0.3207097500142294
88-89	0.36220621065315817
90-91	0.34133636116417765
92-93	0.3315338561011716
94-95	0.33895262544166
96-97	0.3390012731094665
98	0.3343554208339475
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
98	2055597.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.92072749139625
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.05459175098433	38.66102142049046
2	12.023923030410447	12.726295081397494
3	5.368812066816625	8.523643210215674
4	2.9839517818498673	6.3165159637897235
5	1.8089979657187278	4.786674418814548
6	1.1446928915430226	3.6346788344812015
7	0.8130986781340502	3.0120841498402604
8	0.5645405956465394	2.390071921603282
9	0.4175444458710059	1.9887080251937
>10	1.7622247124514754	15.02311665982607
>50	0.04251381333886335	1.4971127310500862
>100	0.014737807051188814	1.2310390315929705
>500	1.852300919084783E-4	0.06749028972969508
>1k	1.8523009190844715E-4	0.14154826197482598
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	4.8647667806481524E-5
6	0.0	0.0	0.0	0.0	4.8647667806481524E-5
7	0.0	0.0	0.0	0.0	4.8647667806481524E-5
8	0.0	0.0	0.0	0.0	4.8647667806481524E-5
9	0.0	0.0	0.0	0.0	4.8647667806481524E-5
10-11	0.0	0.0	0.0	0.0	4.8647667806481524E-5
12-13	0.0	0.0	0.0	4.8647667806481524E-5	4.8647667806481524E-5
14-15	0.0	0.0	0.0	4.8647667806481524E-5	4.8647667806481524E-5
16-17	0.0	0.0	0.0	4.8647667806481524E-5	4.8647667806481524E-5
18-19	0.0	0.0	0.0	4.8647667806481524E-5	4.8647667806481524E-5
20-21	0.0	0.0	0.0	1.4594300341944457E-4	9.729533561296305E-5
22-23	0.0	0.0	0.0	6.810673492907413E-4	9.729533561296305E-5
24-25	0.0	0.0	0.0	0.0018729352105495388	9.729533561296305E-5
26-27	0.0	0.0	0.0	0.003453984414260188	9.729533561296305E-5
28-29	0.0	0.0	0.0	0.0096079143917801	9.729533561296305E-5
30-31	0.0	0.0	0.0	0.03363986228818197	9.729533561296305E-5
32-33	0.0	0.0	0.0	0.0818740249183084	9.729533561296305E-5
34-35	0.0	0.0	0.0	0.15343474426164272	9.729533561296305E-5
36-37	0.0	0.0	0.0	0.2684135071222618	9.729533561296305E-5
38-39	0.0	0.0	0.0	0.44690180030424254	9.729533561296305E-5
40-41	0.0	0.0	0.0	0.6493004222131089	9.729533561296305E-5
42-43	0.0	0.0	0.0	0.8582178316080438	9.729533561296305E-5
44-45	0.0	0.0	0.0	1.1101397793439083	9.729533561296305E-5
46-47	0.0	0.0	0.0	1.414893094317612	9.729533561296305E-5
48-49	0.0	0.0	0.0	1.7417567743093612	9.729533561296305E-5
50-51	0.0	0.0	0.0	2.085720109535089	9.729533561296305E-5
52-53	0.0	0.0	0.0	2.4343536208702385	9.729533561296305E-5
54-55	0.0	0.0	0.0	2.8302483414793853	9.729533561296305E-5
56-57	0.0	0.0	0.0	3.2958308462213166	9.729533561296305E-5
58-59	0.0	0.0	0.0	3.788534425765362	9.729533561296305E-5
60-61	0.0	0.0	0.0	4.306923973911228	9.729533561296305E-5
62-63	0.0	0.0	0.0	4.827964819952549	9.729533561296305E-5
64-65	0.0	0.0	0.0	5.388872429761281	9.729533561296305E-5
66-67	0.0	0.0	0.0	6.029100061928482	9.729533561296305E-5
68-69	0.0	0.0	0.0	6.736680390173754	9.729533561296305E-5
70-71	0.0	0.0	0.0	7.492227318876219	9.729533561296305E-5
72-73	0.0	0.0	0.0	8.239747382390615	9.729533561296305E-5
74-75	0.0	0.0	0.0	9.004294129637278	9.729533561296305E-5
76-77	0.0	0.0	0.0	9.832009873530659	9.729533561296305E-5
78-79	0.0	0.0	0.0	10.742183414356024	9.729533561296305E-5
80-81	0.0	4.8647667806481524E-5	0.0	11.68507251178125	9.729533561296305E-5
82-83	0.0	4.8647667806481524E-5	0.0	12.646374751471226	9.729533561296305E-5
84-85	0.0	4.8647667806481524E-5	0.0	13.608212115507076	9.729533561296305E-5
86	0.0	4.8647667806481524E-5	4.8647667806481524E-5	14.338462256950171	9.729533561296305E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2675	0.0	40.777565	1
GTATCAA	4540	0.0	36.29309	1
TCAACGC	5585	0.0	28.91187	4
ATCAACG	5600	0.0	28.752512	3
TATCAAC	5705	0.0	28.464548	2
CAACGCA	5740	0.0	27.891396	5
AACGCAG	6000	0.0	26.678871	6
GTACATG	7390	0.0	23.791164	1
ACGCAGA	6885	0.0	23.044662	7
TACATGG	7585	0.0	22.8004	2
CGCAGAG	7020	0.0	22.533428	8
ACATGGG	7550	0.0	21.630161	3
GTATAGG	515	0.0	21.448689	1
GTATTAG	540	0.0	19.603374	1
GACCGCG	285	3.0468073E-9	19.307878	7
CATGGGG	5000	0.0	19.083147	4
GCAGAGT	7820	0.0	18.996012	9
GAGTACA	5450	0.0	18.325676	1
AGAGTAC	6070	0.0	17.601065	10-11
GTATATA	665	0.0	17.30275	1
>>END_MODULE