FastQCFastQC Report
Mon 15 Aug 2016
SRR2920869_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920869_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2055597
Sequences flagged as poor quality0
Sequence length101
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26150.043.095061
GTATCAA43100.036.4072231
TCAACGC54400.028.533024
ATCAACG55850.027.8772263
CAACGCA56100.027.4118735
TATCAAC57050.027.3720552
AACGCAG58550.026.3458986
GTACATG75450.025.5238761
TACATGG78800.024.0333212
ACATGGG77300.023.7645933
TCGCGTA1652.4499532E-623.0101959
ACGCAGA67750.022.207317
CGCAGAG70100.021.4612738
CATGGGG50350.019.3265044
GCAGAGT79500.018.3267529
AGAGTAC63300.018.1048910-11
GAGTACA54500.017.5368021
GTATATA7100.016.7429711
CGTAGAC2304.2984662E-516.5105323
CAGAGTA71200.016.36266510-11
Produced by FastQC (version 0.11.5)