##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920869_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2055597
Sequences flagged as poor quality	0
Sequence length	101
%GC	48
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.29912623923853	31.0	30.0	34.0	27.0	34.0
2	30.314848192520227	31.0	30.0	34.0	26.0	34.0
3	30.447159146466937	31.0	30.0	34.0	27.0	34.0
4	34.09863363295432	35.0	35.0	37.0	30.0	37.0
5	33.638908793892966	35.0	35.0	37.0	28.0	37.0
6	33.58570138018298	35.0	35.0	37.0	28.0	37.0
7	33.40885154045272	35.0	35.0	37.0	28.0	37.0
8	33.343136324873015	35.0	33.0	37.0	27.0	37.0
9	34.68204030264687	38.0	35.0	39.0	27.0	39.0
10-11	34.48216284612208	37.5	34.0	39.0	27.0	39.0
12-13	34.31228008213672	37.0	34.0	39.0	27.0	39.0
14-15	35.204363987688254	38.0	34.0	40.0	27.0	41.0
16-17	35.08102171777834	38.0	34.0	40.0	26.0	41.0
18-19	34.99060394620152	38.0	34.0	40.0	25.5	41.0
20-21	34.881361473090294	38.0	34.0	40.0	25.0	41.0
22-23	34.73325486464516	38.0	34.0	40.0	25.0	41.0
24-25	34.55461406102461	38.0	33.0	40.0	24.0	41.0
26-27	34.37508008622313	38.0	33.0	40.0	24.0	41.0
28-29	34.30881734114226	38.0	33.0	40.0	23.5	41.0
30-31	34.29671769320542	38.0	33.0	40.0	23.5	41.0
32-33	34.14854468069373	38.0	33.0	40.0	23.0	41.0
34-35	33.97636112525947	38.0	33.0	40.0	22.0	41.0
36-37	33.78478612296087	38.0	33.0	40.0	20.5	41.0
38-39	33.600399056819015	38.0	32.5	40.0	19.0	41.0
40-41	33.36863329728541	38.0	32.0	40.0	18.0	41.0
42-43	33.3936192259475	38.0	32.0	40.0	18.0	41.0
44-45	33.2426419186251	38.0	32.0	40.0	15.5	41.0
46-47	32.984434448970305	38.0	32.0	40.0	15.0	41.0
48-49	32.7123614210373	37.0	31.0	40.0	12.5	41.0
50-51	32.44782197094081	37.0	31.0	40.0	9.0	41.0
52-53	32.18477040976417	37.0	31.0	40.0	8.0	41.0
54-55	31.889547416152098	36.0	30.5	40.0	7.5	41.0
56-57	31.560120247305285	36.0	30.0	39.0	4.5	41.0
58-59	31.216969328131924	35.5	29.5	39.0	2.0	40.0
60-61	30.877343418967826	35.0	29.0	39.0	2.0	40.0
62-63	30.549152873836654	35.0	29.0	39.0	2.0	40.0
64-65	30.127342324395297	35.0	28.0	38.0	2.0	40.0
66-67	29.67958651428271	34.0	28.0	38.0	2.0	40.0
68-69	29.229362564743965	34.0	28.0	37.0	2.0	39.5
70-71	28.7970010658704	34.0	26.5	36.5	2.0	39.0
72-73	28.35659226978829	34.0	26.0	36.0	2.0	39.0
74-75	27.91571353723517	33.0	26.0	35.5	2.0	38.5
76-77	26.47039229965796	31.5	24.5	34.0	2.0	36.0
78-79	27.313589190877394	33.0	26.0	35.0	2.0	37.0
80-81	27.34233655721428	33.0	26.0	35.0	2.0	36.5
82-83	27.012674663370298	33.0	25.5	35.0	2.0	36.0
84-85	26.700262502815484	33.0	25.0	35.0	2.0	36.0
86-87	26.162397833816648	32.0	24.0	35.0	2.0	35.0
88-89	25.803875467808133	32.0	22.5	35.0	2.0	35.0
90-91	25.438863989390917	32.0	20.5	34.5	2.0	35.0
92-93	25.12913936924407	32.0	19.5	34.0	2.0	35.0
94-95	24.760488802036583	32.0	17.5	34.0	2.0	35.0
96-97	24.226738023065806	31.5	6.0	34.0	2.0	35.0
98-99	23.62715746325763	31.0	2.0	34.0	2.0	35.0
100-101	21.42084635266543	28.0	2.0	33.0	2.0	34.5
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	44722.0
3	20630.0
4	11351.0
5	8041.0
6	6924.0
7	6950.0
8	7887.0
9	9290.0
10	10513.0
11	11977.0
12	13302.0
13	14415.0
14	15800.0
15	16879.0
16	18214.0
17	19115.0
18	18455.0
19	17260.0
20	17174.0
21	17739.0
22	19594.0
23	22217.0
24	25566.0
25	30431.0
26	35757.0
27	42687.0
28	49917.0
29	58854.0
30	69323.0
31	82340.0
32	98500.0
33	120606.0
34	151817.0
35	196124.0
36	261906.0
37	303725.0
38	167951.0
39	11643.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.29035984266935	19.261744966442954	13.747032991670444	24.700862199217248
2	16.035828034386117	20.688831517072657	38.07925386153025	25.196086587010974
3	19.21341311818216	24.53607906348325	29.251649710196897	26.998858108137696
4	13.801065909482352	16.603822055198812	35.73920345792946	33.85590857738937
5	14.510771206725437	36.95944499581606	34.026942611944655	14.502841185513846
6	33.481408470183524	35.477403526337476	16.780003720546084	14.26118428293292
7	29.165499512109093	31.56880674502476	20.720772947801276	18.544920795064872
8	26.87079385845495	34.54855318715756	19.827149104841023	18.753503849546465
9	26.844971262827965	15.582523278619526	19.316553176190677	38.25595228236184
10-11	25.72760578436828	25.395164108377156	27.50640739203012	21.37082271522445
12-13	26.33298916854983	22.616301722122653	27.494667069274527	23.556042040052986
14-15	23.51098598573613	24.308461377551804	24.833512279070604	27.34704035764146
16-17	23.577865043887	27.446838339776065	25.52979112115207	23.445505495184868
18-19	23.698801005466972	26.472890805176895	26.965473222358057	22.862834966998076
20-21	24.47868774115338	25.772293053687672	26.620826892235083	23.12819231292386
22-23	24.209997412242018	25.581143983779164	26.520380845916907	23.688477758061914
24-25	23.821924479433495	25.89576963430807	26.556661598733072	23.725644287525363
26-27	23.76561051412805	25.991127237996857	26.63637034082028	23.606891907054813
28-29	23.731488993284476	25.974928837044743	26.627476955077185	23.666105214593603
30-31	24.04083882900273	25.946363758896755	26.39166990656916	23.62112750553135
32-33	23.70991543600156	26.066563582369913	26.29214057259231	23.93138040903622
34-35	23.965369633617904	26.011822261731943	26.45238969331554	23.570418411334618
36-37	24.13912822606427	25.74058548087998	26.317346577801437	23.80293971525432
38-39	23.943073902221226	25.726454965947994	26.43168065325349	23.898790478577286
40-41	23.992667932471797	25.69666319884073	26.502932729691565	23.807736138995914
42-43	24.042186837850608	25.536591880731734	26.42138767781182	23.99983360360584
44-45	23.860761818888278	25.71155170740051	26.418876061795505	24.00881041191571
46-47	24.003225456384072	25.569674091672457	26.297056357404703	24.130044094538768
48-49	23.738184850531184	25.641039363397706	26.334087764399545	24.28668802167157
50-51	23.587922512691105	25.834333400558613	26.351124677263932	24.226619409486354
52-53	23.815878549725035	25.6845857019249	26.340862634784408	24.15867311356566
54-55	23.693457263684785	25.574598559465358	26.342400411025285	24.38954376582457
56-57	23.665144722535068	25.730934548293956	26.139037745349636	24.464882983821337
58-59	23.440759515840274	25.5751585973379	26.405556744765313	24.578525142056513
60-61	23.47804825236329	25.659975694997765	26.357035373631625	24.504940679007316
62-63	23.29598253946476	25.74584820894259	26.27623726633252	24.68193198526013
64-65	23.30299367462267	25.631193745604676	26.24456293158118	24.82124964819148
66-67	23.22415222087737	25.725477198940403	26.075300608200212	24.975069971982006
68-69	23.38400098160656	25.478289745327633	26.130673452216797	25.007035820849012
70-71	23.209775261818255	25.198854388518193	26.257075527930606	25.334294821732943
72-73	23.12089763659579	25.270522067986594	26.181781193171023	25.4267991022466
74-75	23.08747349824612	25.348786086387502	26.022561759524137	25.541178655842238
76-77	22.968634682298244	25.130533390581505	26.397795311452732	25.50303661566752
78-79	22.973944393121133	25.254598101970693	26.169848914725	25.60160859018317
80-81	22.90341360122437	25.204262129892346	26.09380743637008	25.7985168325132
82-83	22.71127248807634	25.15083680919271	26.245872391645868	25.892018311085074
84-85	22.598061765915794	25.068401607629355	26.211870680274572	26.121665946180272
86-87	22.526677425915548	25.0668795105566	26.33082300450318	26.075620059024672
88-89	22.557731291086718	24.893417460395938	26.229462453917034	26.319388794600307
90-91	22.442077444049314	24.855341469737986	26.270769550455555	26.43181153575715
92-93	22.431406282877408	24.77268056059354	26.26433546564053	26.531577690888525
94-95	22.20075482639382	24.685634947352852	26.40194554119243	26.7116646850609
96-97	22.165404608599832	24.584007336396652	26.449826198428124	26.800761856575388
98-99	22.09612860932291	24.55272430069654	26.357151631393887	26.993995458586667
100-101	22.21590851042403	24.42460219966908	26.375863479820637	26.983625810086252
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	135.0
1	88.5
2	77.0
3	110.5
4	90.5
5	91.0
6	147.5
7	291.5
8	456.5
9	480.0
10	408.5
11	352.0
12	361.0
13	404.0
14	440.5
15	495.5
16	556.5
17	593.5
18	646.0
19	729.5
20	816.5
21	944.0
22	1107.0
23	1318.0
24	1579.5
25	1998.0
26	2633.0
27	3443.0
28	4695.5
29	6565.0
30	8995.0
31	12183.5
32	15954.5
33	19577.0
34	23283.0
35	28012.0
36	34617.0
37	40748.0
38	45716.5
39	50445.5
40	54949.5
41	59553.5
42	63079.5
43	67585.0
44	73307.0
45	77808.0
46	81615.5
47	85607.5
48	89049.0
49	91543.5
50	92102.0
51	90991.0
52	88932.0
53	87705.0
54	86565.5
55	82805.0
56	77093.0
57	69859.5
58	62769.0
59	55757.0
60	47250.5
61	38550.5
62	30814.5
63	23468.5
64	17233.0
65	12790.5
66	9639.5
67	7284.5
68	5351.5
69	3685.0
70	2582.5
71	1919.5
72	1395.5
73	1066.5
74	828.0
75	613.0
76	448.5
77	271.5
78	110.0
79	43.0
80	19.5
81	10.0
82	7.5
83	5.0
84	2.0
85	1.0
86	1.0
87	1.5
88	2.0
89	2.0
90	0.5
91	1.0
92	1.0
93	0.0
94	0.5
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.18812053140766405
2	0.0
3	0.01148084960232964
4	0.012794336633104642
5	0.005691777133358338
6	0.10386277076683806
7	0.1402998739538927
8	0.12152187418059085
9	0.13888909158750476
10-11	0.11210855046003666
12-13	0.1127166463076177
14-15	0.115173353531845
16-17	0.06538246553191117
18-19	0.07910110785333896
20-21	0.036899256031216236
22-23	0.08248212076588941
24-25	0.057233981174325516
26-27	0.06482301735213664
28-29	0.03921002025202411
30-31	0.02403194789640187
32-33	0.031353421901277344
34-35	0.01977527696333474
36-37	0.024323833903240763
38-39	0.03171827940982595
40-41	0.025053548920337988
42-43	0.012842984300911122
44-45	0.025710292435725486
46-47	0.03376148145769818
48-49	0.008999818544199081
50-51	0.015494282196364364
52-53	0.006737701991197691
54-55	0.012113269283813899
56-57	0.005448538794325931
58-59	0.0037458704210990776
60-61	0.02259684169611067
62-63	0.010653839249619454
64-65	0.0071268833336495425
66-67	0.04115592696428337
68-69	0.08858740307560285
70-71	0.021891450512916685
72-73	0.04439099687341439
74-75	0.08384425546447091
76-77	0.23723035205830714
78-79	0.07902813635162924
80-81	0.01622399721346159
82-83	0.01118896359549075
84-85	0.013256489477266216
86-87	0.25627591400454464
88-89	0.6233712152722543
90-91	0.26807297344761644
92-93	0.3535712496175077
94-95	0.001216191695162038
96-97	0.0044755854381963
98-99	0.003381012912550466
100-101	0.005156652787487042
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2055597.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.046892303521
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.35665962479315	35.21132167077507
2	13.10630215293289	13.118593846904755
3	5.980573722551432	8.979273870173975
4	3.254869267702556	6.515843668110002
5	1.969022714435383	4.927173386626708
6	1.2678544199939155	3.8071304168387137
7	0.9038003735249742	3.1662679970380503
8	0.6237895915179638	2.4974984405405505
9	0.48079386315311057	2.1656014820475784
>10	1.9875732738673908	16.196980759824264
>50	0.050682700113472444	1.6994355477240974
>100	0.01749247221907215	1.3677463302662891
>500	1.95274398219115E-4	0.07622843886299398
>1k	3.9054879643823E-4	0.2709041442669478
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	9.729533561296305E-5	0.0
22-23	0.0	0.0	0.0	5.837720136777783E-4	0.0
24-25	0.0	0.0	0.0	0.0017513160410333349	0.0
26-27	0.0	0.0	0.0	0.0033080414108407436	0.0
28-29	0.0	0.0	0.0	0.009145761547618526	0.0
30-31	0.0	0.0	0.0	0.033129061776213914	0.0
32-33	0.0	0.0	4.8647667806481524E-5	0.08172808191488895	0.0
34-35	0.0	0.0	4.8647667806481524E-5	0.1536050110989654	0.0
36-37	0.0	0.0	4.8647667806481524E-5	0.26787838277639053	0.0
38-39	0.0	0.0	4.8647667806481524E-5	0.4475828676535333	0.0
40-41	0.0	0.0	4.8647667806481524E-5	0.6512706527592713	0.0
42-43	0.0	0.0	4.8647667806481524E-5	0.8635204273989503	0.0
44-45	0.0	0.0	4.8647667806481524E-5	1.1188720357151718	0.0
46-47	0.0	0.0	4.8647667806481524E-5	1.4259361149096832	2.4323833903240762E-5
48-49	0.0	0.0	4.8647667806481524E-5	1.753043033240465	4.8647667806481524E-5
50-51	0.0	0.0	4.8647667806481524E-5	2.0950847855878365	4.8647667806481524E-5
52-53	0.0	0.0	4.8647667806481524E-5	2.4413345612004687	4.8647667806481524E-5
54-55	0.0	0.0	4.8647667806481524E-5	2.832315867361161	4.8647667806481524E-5
56-57	0.0	0.0	4.8647667806481524E-5	3.292936309986831	4.8647667806481524E-5
58-59	0.0	0.0	4.8647667806481524E-5	3.7822832004522287	4.8647667806481524E-5
60-61	0.0	0.0	4.8647667806481524E-5	4.299067375560482	4.8647667806481524E-5
62-63	0.0	0.0	4.8647667806481524E-5	4.817043418529995	4.8647667806481524E-5
64-65	0.0	0.0	4.8647667806481524E-5	5.371480888520464	4.8647667806481524E-5
66-67	0.0	0.0	4.8647667806481524E-5	6.0032681503232395	4.8647667806481524E-5
68-69	0.0	0.0	4.8647667806481524E-5	6.706153978625188	4.8647667806481524E-5
70-71	0.0	0.0	4.8647667806481524E-5	7.456179397031617	4.8647667806481524E-5
72-73	0.0	0.0	4.8647667806481524E-5	8.20121842948788	4.8647667806481524E-5
74-75	0.0	0.0	4.8647667806481524E-5	8.96445168970377	4.8647667806481524E-5
76-77	2.4323833903240762E-5	0.0	4.8647667806481524E-5	9.791826899922505	4.8647667806481524E-5
78-79	4.8647667806481524E-5	0.0	4.8647667806481524E-5	10.701903145412256	4.8647667806481524E-5
80-81	4.8647667806481524E-5	0.0	4.8647667806481524E-5	11.64231121177935	4.8647667806481524E-5
82-83	4.8647667806481524E-5	0.0	4.8647667806481524E-5	12.59835950334623	4.8647667806481524E-5
84-85	4.8647667806481524E-5	0.0	4.8647667806481524E-5	13.560975230066983	4.8647667806481524E-5
86-87	4.8647667806481524E-5	0.0	4.8647667806481524E-5	14.5518309279494	4.8647667806481524E-5
88-89	4.8647667806481524E-5	0.0	4.8647667806481524E-5	15.614393288178569	9.729533561296305E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2615	0.0	43.09506	1
GTATCAA	4310	0.0	36.407223	1
TCAACGC	5440	0.0	28.53302	4
ATCAACG	5585	0.0	27.877226	3
CAACGCA	5610	0.0	27.411873	5
TATCAAC	5705	0.0	27.372055	2
AACGCAG	5855	0.0	26.345898	6
GTACATG	7545	0.0	25.523876	1
TACATGG	7880	0.0	24.033321	2
ACATGGG	7730	0.0	23.764593	3
TCGCGTA	165	2.4499532E-6	23.010195	9
ACGCAGA	6775	0.0	22.20731	7
CGCAGAG	7010	0.0	21.461273	8
CATGGGG	5035	0.0	19.326504	4
GCAGAGT	7950	0.0	18.326752	9
AGAGTAC	6330	0.0	18.10489	10-11
GAGTACA	5450	0.0	17.536802	1
GTATATA	710	0.0	16.742971	1
CGTAGAC	230	4.2984662E-5	16.510532	3
CAGAGTA	7120	0.0	16.362665	10-11
>>END_MODULE