FastQCFastQC Report
Mon 15 Aug 2016
SRR2920868_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920868_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3505137
Sequences flagged as poor quality0
Sequence length98
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA28650.043.2134061
GTATCAA51350.041.677661
ATCAACG64700.032.3234523
TCAACGC65400.031.7671034
CAACGCA65500.031.5785665
TATCAAC68650.030.598542
AACGCAG69500.030.0207656
GTACATG102750.025.5319421
ACGCAGA82000.025.330427
TACATGG103150.024.944192
CGCAGAG86300.023.906868
ACATGGG106550.023.3724123
GCAGAGT96800.020.5087669
CATGGGG73350.019.8831024
GAGTACA78150.018.6690031
GTATAGG8750.018.4098741
AGAGTAC86650.017.93811210-11
AGTACAT76700.017.2813742
GTGTAGC20500.015.9402561
CAGAGTA95200.015.89361310-11
Produced by FastQC (version 0.11.5)